Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 285,882 | T→G | 14.3% | I163S (ATC→AGC) | yagG → | CP4‑6 prophage; putative sugar transporter |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 285,882 | 0 | T | G | 14.3% | 240.2 / 37.5 | 91 | I163S (ATC→AGC) | yagG | CP4‑6 prophage; putative sugar transporter |
Reads supporting (aligned to +/- strand): ref base T (41/37); new base G (6/7); total (47/44) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.68e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-02 |
GGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTT > NC_000913/285612‑286152 | ggCACGGCCAGTTACGCCCGTGCCTGCTGGGGGGGGCCATCCCGTTCGGCATGGGCTGCTTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGGGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCTGCATcgcg < 4:384169/275‑1 (MQ=255) gCCGGTTCGGCCCGTTCCTGCTGTGGGGGGCGATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACCCGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACGTTCTCCTGACCCTGGTCTACACCGTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCg < 4:337375/275‑1 (MQ=255) gTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACCTTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCCCGCCCTGCAGTCCTGGCCCTTCTTCCTGGCGGCGGCGGCGTCGCCCGCTATCAGCGGCATcggcctcccccgg > 4:369400/1‑263 (MQ=255) ttCCTGCTGTGGGGGGCCATCCCGGTCGGAATCGTCTGCGGGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCAGCTCCGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGcatcat < 4:392691/275‑1 (MQ=255) gtgtgGGGGGCGCTACCGTTCGGCATCTTCTGCGTGCTGACCTTCTACCCGCCGGACTTCTCCGCACAGGGCAAGTTCATCTACGCCTGCGTGACCTACATTCTCCGGACCCTGGTCTACACCTGCGTTATCGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaa < 4:498793/274‑1 (MQ=255) gggggCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCCGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAACgggg > 2:224222/1‑275 (MQ=255) ggCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTCCGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCCCGCCCTGCAGTCCTGGAGCTTCTTCCTGGCGGCGGCGGGCTCGCGCGCTATCCGCGGCATCGCGCTGCCGCTG‑‑‑GTGCGCATCATAGGCCAAGGGGa > 1:91473/1‑273 (MQ=255) gcccgtCCCGTTCGGCCTCGTCTGCGGGCTGCCCTTCTACACGCCGTACTTCTCCGCACAGGGCAATATCATCTACGNGTGCGTGACCTACATTCTCCTGCCCCTGGTCTACACCTTCGTTAACGGGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACg < 3:129533/270‑1 (MQ=255) gTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGcc > 2:124888/1‑273 (MQ=255) tGACCGTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAACCTGGTCTACACCTTCGTTCACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg < 1:109409/274‑1 (MQ=255) cGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATAATCGTCAAAGGGGCCGAGCAGCTGGGCTACTTCGGCGCCTTGTGCGTGCTGGGGCTggc > 1:19527/1‑272 (MQ=255) ctcatcCGCACAGGGCAGGTTCATCAACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTACGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATTACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAgcggcg < 2:511364/271‑1 (MQ=255) cTTCTCCGGCCGGGGCAAGTTCATCGACGCCGGCGGGACCTACATCCTCCTGACCCTGGTCTACACCTGCGTTAACGGGCCGTACTGCGCCATGCCGGGCGTCAGCACCGCCGAGCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCGCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAgcggcg < 4:492460/275‑1 (MQ=255) ctcCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCCCGCCCGCCATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCCTCCTCGGCAAAGGGGACGAGACGTTGGGCTCCTTCGGCGCCATGTGCGTGCTGGGGCTGCggggcggg > 4:255953/1‑272 (MQ=255) cGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACAGTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgc < 2:487975/275‑1 (MQ=255) acaGGGCAAGATCATCTACGGCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctgc < 2:100682/275‑1 (MQ=255) ggCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGccct > 2:159371/1‑274 (MQ=255) aGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg < 2:178936/238‑1 (MQ=255) tcTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGCGCATCATCGGCCAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCCTGTGCGTGCTGGGGCTGAGCGGCGTGGTTCTGCTCTACCTcgtagtc > 1:12028/1‑269 (MQ=255) aCGCCTGTGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGGGCTGCTCTACGTCTGcttctt > 2:44596/1‑274 (MQ=255) gCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCa < 2:369401/274‑1 (MQ=255) cGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCCCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGccc > 2:68772/1‑274 (MQ=255) tACCTTCTCCTGCCCCTGGTCTACACCTTCGTTGACGTGCCGTACTGCGCCAGGCCGAGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTCAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcg < 4:205676/275‑1 (MQ=255) atttcccTGACCCTTGTTCACCCCTTCGTTACCGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTa < 4:44595/270‑1 (MQ=255) gACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCg < 8:897245/148‑1 (MQ=255) cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcg < 6:227359/138‑1 (MQ=255) cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcg > 5:227359/1‑138 (MQ=255) tCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTTTTCCTGGCGGCGGCGGGCTCGCTCGCTATCTGCGGCATCGCGCTGCCTCTG‑‑‑GTGAGCTTCATCGGCACAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTgacg > 2:375651/1‑272 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCCCCGCCGACCCCAAAGAGCGTCCCGCCCTGCAGTCCTGGCCCTTCTTCCTTGCGCCCGCGGGCTCGCCCGCGATCACCCGCCTCGCTCCGCCCCCG‑‑‑TCGGCCATCCTCGGCAACNCTCCCGCCCAGGTGTGCCACTTCTGTCCCATGTGTGTGCTtggtgcccnncgc > 4:544624/1‑196 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCgcgc < 8:1075341/126‑1 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCgcgc > 7:1075341/1‑126 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTTCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCCCGACCAAAGACCGCTACACCTTTGCGGTGCACCCGGGCtc > 2:275773/1‑274 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCCCTACGTCTGCTTCTTCACGACCAAAGAGCGCCAAACCTTTGGGGGGCAGCCGGGCtgg > 4:411009/1‑273 (MQ=255) tGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGCGCGGCGTGGTTCTGCTCCACGTCTGCTTCTTCACGTCAAAAGAGCGCCACCCCTTTGAGGTGCCGCCGGGCTCGCCGGTGGCGAAAGACCTGAGgct > 4:430070/1‑274 (MQ=255) tGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAgct > 2:344465/1‑274 (MQ=255) ccGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCCCGCTCGCGATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCCTCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCCTGTTCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAACGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGCTGGTGGCGTAAGACCTTAAgctgc > 4:444136/1‑274 (MQ=255) catcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg < 5:354337/135‑1 (MQ=255) catcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg > 6:354337/1‑135 (MQ=255) atcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCa > 2:417037/1‑274 (MQ=255) acagccgccgACCCGAAAGGGCGTAACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCGCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCa < 2:521051/270‑1 (MQ=255) aCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgt > 7:926430/1‑148 (MQ=255) aaaGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGCGCATCATCGGCaaa > 6:170249/1‑102 (MQ=255) aaaGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaaa < 5:170249/102‑1 (MQ=255) aaaGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTTCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTTAGGTGCAGCCGGGCTAGTCGGTGGCGAAAGACCTTACACTGTCCAGGGGCACCAACCAGTGgct > 4:521050/1‑271 (MQ=255) cGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTTCAGCCGGGCTCGTCGGTGGCGCAAGCACTTAAGCCGCTTCTGGGCCACAGCCCGTTgcgccacctgtt > 4:67131/1‑267 (MQ=255) cGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATGAtgtg > 2:327593/1‑275 (MQ=255) cACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑Gt > 7:188702/1‑77 (MQ=255) cACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑Gt < 8:188702/77‑1 (MQ=255) tGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGTGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACCGCCAGTGGCGCATCATGTTCGCGTTCaa > 1:116263/1‑274 (MQ=255) gCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTg > 5:1029415/1‑70 (MQ=255) gCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTg < 6:1029415/70‑1 (MQ=255) cAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctgc < 8:926430/148‑1 (MQ=255) aGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaa < 6:124626/82‑1 (MQ=255) aGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaa > 5:124626/1‑82 (MQ=255) ggCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTg > 7:646381/1‑92 (MQ=255) ggCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTg < 8:646381/92‑1 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGc > 8:344661/1‑90 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGc < 7:344661/90‑1 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTg < 2:255954/275‑1 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTg < 2:67132/275‑1 (MQ=255) ttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGCCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCCCGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGGCGCGCAAGCCCTTAAGCTGCTGCTGGGCAACAGCCAGTGCCGCACACTGTGCCCGTTCACGATGATGTGGACCtc > 4:151436/1‑271 (MQ=255) ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGcatca > 8:235788/1‑56 (MQ=255) ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGcatca < 7:235788/56‑1 (MQ=255) ggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg > 8:682003/1‑93 (MQ=255) ggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg < 7:682003/93‑1 (MQ=255) tcgctATCATCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCg < 2:328748/275‑1 (MQ=255) cgctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTg < 6:332689/94‑1 (MQ=255) cgctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTg > 5:332689/1‑94 (MQ=255) gctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCt < 8:575220/148‑1 (MQ=255) gctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCt > 7:575220/1‑148 (MQ=255) ctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTCCAGCCTGGCTCGTCGGTGGCCAAAGCCCTTAAGCTGCTGCTGGGCAACACCCAGTGCCGCATCATGTGCTCGTCCAACATGATTGCGACCTTCTCCACCGTGGTTCGCGGCGGGGCGCCg > 4:290070/1‑275 (MQ=255) tATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACg < 2:127502/274‑1 (MQ=255) accAGCGGCAGCGCGATGCCGCTG‑‑‑ATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATgtgcg > 8:1025917/3‑79 (MQ=37) accAGCGGCAGCGCGATGCCGCTG‑‑‑ATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATgtgcg < 7:1025917/77‑1 (MQ=38) ccAGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTc > 7:193549/2‑113 (MQ=255) ccAGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTc < 8:193549/112‑1 (MQ=255) aGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggtg > 5:744373/1‑99 (MQ=255) aGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggtg < 6:744373/99‑1 (MQ=255) aGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAgg > 7:171459/1‑148 (MQ=255) gCGGCAGCGCGATGCCGCTGATAGCGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCt < 7:633498/88‑1 (MQ=25) gCGGCAGCGCGATGCCGCTGATAGCGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCt > 8:633498/1‑88 (MQ=25) gCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGt < 8:171459/148‑1 (MQ=255) cGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCt < 8:154627/134‑1 (MQ=255) cGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCt > 7:154627/1‑134 (MQ=255) cGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaa > 7:412329/1‑126 (MQ=255) cGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaa < 8:412329/126‑1 (MQ=255) ggCATCGCGCTGCCGCTG‑‑‑GGGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACtt < 2:474003/275‑1 (MQ=255) cATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCg < 8:273177/59‑1 (MQ=255) cATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCg > 7:273177/1‑59 (MQ=255) aTCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACAcc > 5:789384/1‑135 (MQ=255) aTCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACCACCAAAGAGCGCTAcccc < 6:789384/135‑1 (MQ=255) | GGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTT > NC_000913/285612‑286152 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |