Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,081,688 | A→T | 10.5% | I287N (ATT→AAT) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,081,688 | 0 | A | T | 10.5% | 292.8 / 23.7 | 95 | I287N (ATT→AAT) | dacD | D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b |
Reads supporting (aligned to +/- strand): ref base A (44/41); new base T (5/5); total (49/46) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-03 |
Only 100 of 103 total aligned reads displayed.
ATGCTGCCTTCCCCGACAGATTCCAGGGTA‑ACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCT > NC_000913/2081421‑2081937 | ctgctgcCCTTCCCGAATGATTGCACGGTNTACCTGGGTCCAGTTCGCCCCCTGTNTATCACGGTCGCAAGGTTCAATTTCCCCTCCCCGCTTATGTGCGCGAATTTGCGCGGTGAGTTCTTTACCATCAAGGGTANTTTTGGCTTTGCTATGGGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTCTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGTGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa < 3:100120/274‑1 (MQ=255) aTGCTGCCTTCCCCGACAGATTCCAGGGTA‑ACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGCTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc > 4:363196/1‑275 (MQ=255) gcCTTCCCCGACAGATTCCAGGGTA‑ACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCg < 2:73749/272‑1 (MQ=255) ccccGACAGATTCCAGGGTA‑ACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAg < 2:213778/275‑1 (MQ=255) tCCAGGGTA‑ACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCCAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTACAAAATTTGCACCGA‑TGTAAGGTTTGGTTcccc > 4:305661/1‑275 (MQ=255) aCCAGCGGCCAGTGCGCCACCTGTTTATTACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGCCGCGGTGAGCTCTTTACCATCAAGGGTCTATTTGGCTTTGATATGTGGAATTTCGGCCTTCGGTAGCACCATCCAGAACTCTTGCTCCGTTCCCAGGTCGATCTTTCATTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTCCAAAATTTCCACCATCAGCAAA‑TTTTTTTGCCCCCAACGCa > 4:183276/1‑274 (MQ=255) tCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTGTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTACTTTTCTTGCCTCTTCCTCCCGCCCTTTTGc > 4:105031/1‑275 (MQ=255) tCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt > 2:501590/1‑207 (MQ=255) tAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACt < 2:435856/275‑1 (MQ=255) aaaGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa < 4:501589/207‑1 (MQ=255) ttCACTTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg < 1:159466/275‑1 (MQ=255) ttCAATTTCCCCTCCCCGCTGATGGGCGCTAATGGGGGCGGGTAGTTCTTTACCCTCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg < 4:77960/275‑1 (MQ=255) cAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGcccc > 4:486590/1‑224 (MQ=255) cAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGc > 2:504088/1‑274 (MQ=255) cAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTGCCTCACGACCTTTTGCACTGTCAGcc < 2:105032/275‑1 (MQ=255) cAATTTCCCCTACCCGCGGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGcccc < 2:486591/224‑1 (MQ=255) cgcgGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCAt > 4:376360/1‑275 (MQ=255) gcgGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCTGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATc < 2:296087/275‑1 (MQ=255) tGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACa > 2:203860/1‑275 (MQ=255) aGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt > 2:560303/1‑156 (MQ=255) cTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa < 4:560302/156‑1 (MQ=255) atatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGc > 6:825755/1‑139 (MQ=255) atatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGc < 5:825755/139‑1 (MQ=255) tatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑GGTAAAGTTTTGTTGCCCCCAACCCCGTAATTTTCTTGCCTCTTCCTCCCCACCTTTTGCACTGTCAGCCCCCATTACCACTTCAATGAGACGACGCTGCCCCTCTACAGCCGCAGCAATGCTCTTAAAccc > 1:88427/1‑275 (MQ=255) gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGCCGACGCTTCCCATCTACAGCCGAAGCAATGAAATTAAACCCGGCAcc > 4:218705/1‑275 (MQ=255) gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCAcc > 2:151520/1‑275 (MQ=255) tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGATGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAg > 4:289858/1‑275 (MQ=255) tgtGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCTATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAGTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCGTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATGTACAGACGAAGCAATGAGATTAAACCCGGCACCAg < 2:183277/273‑1 (MQ=255) tGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGaa > 2:437626/1‑275 (MQ=255) attgtgAATTTCGGCTTTCGGTGGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATAACAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTAt < 2:385072/270‑1 (MQ=255) ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTttgt < 7:318666/138‑1 (MQ=255) ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTttgt > 8:318666/1‑138 (MQ=255) ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAAc > 7:1111682/1‑148 (MQ=255) tCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTa < 8:1111682/148‑1 (MQ=255) tttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGc < 2:218706/275‑1 (MQ=255) tAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGc < 6:1091668/103‑1 (MQ=255) tAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGc > 5:1091668/1‑103 (MQ=255) aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGttt > 5:555926/1‑114 (MQ=255) aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGttt < 6:555926/114‑1 (MQ=255) gaTCAAGAACTTTTGTTCCATTCGCAGGTCGATATTTTCGTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTCTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCGTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCa < 2:240051/274‑1 (MQ=255) aTCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTttgttg > 5:13648/1‑116 (MQ=255) aTCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTttgttg < 6:13648/116‑1 (MQ=255) tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGaa > 4:367157/1‑242 (MQ=255) tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAAAAGTATGACCCGTTTTCAGGCCGTCAACATTCAt < 2:363197/274‑1 (MQ=255) gAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTAt < 2:367158/242‑1 (MQ=255) aaCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTaa > 6:928515/1‑100 (MQ=255) aaCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTaa < 5:928515/100‑1 (MQ=255) ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt < 6:1117397/148‑1 (MQ=255) ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt > 5:1117397/1‑148 (MQ=255) ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAttt > 1:32238/1‑83 (MQ=255) ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGTCCTTTTGCACTGGCCGCCCCCATTACCACTGCAATGACACGACTCTGCCCATCTACACCCGAAGCAATGAGATTAAACCCGGCACCAAAATCAAGACCCGTTTTCAGCCCGTCAACATTCAGGGTTTTAAccc > 4:240050/1‑275 (MQ=255) ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATcc > 2:29795/1‑274 (MQ=255) gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTTAGGCCGTCAACATTCATGGTTTTATCCcac > 1:72596/1‑275 (MQ=255) tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc < 3:32238/83‑1 (MQ=255) tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgt > 6:883381/1‑86 (MQ=255) tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgt < 5:883381/86‑1 (MQ=255) ttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAACGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCTTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCAACAGCCGAAGCAATCAGATTAAACCCGGCACCAAAAGTATGACCCGTTTCAAGGCCGTCAACCTTCATGGTTTTACCCCAAaaaaac > 4:251397/1‑275 (MQ=255) cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCAcaacaa < 2:305662/272‑1 (MQ=255) gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGaa > 4:426861/1‑214 (MQ=255) ttttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTAt < 2:426862/214‑1 (MQ=255) tCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGc < 7:527015/90‑1 (MQ=255) tCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGc > 8:527015/1‑90 (MQ=255) ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAAtt > 4:565523/1‑94 (MQ=255) tCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGATGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGAt < 2:289859/275‑1 (MQ=255) cGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt < 2:565524/94‑1 (MQ=255) aTACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa < 5:1023858/50‑1 (MQ=255) aTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga > 4:561482/1‑169 (MQ=255) gttccgttccGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGAtt < 2:561483/169‑1 (MQ=255) ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTttgttg > 5:669146/1‑57 (MQ=255) ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTttgttg < 6:669146/57‑1 (MQ=255) ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCCTTACCACTGCAATGAGGCGACGCTGCCCATCTACAGCCGAAGCACTGAGATTAAACCCGGCACCAGACGTATAACCCGTTTTCGGGCCGTCAACATTCATTGTTTTATCTCCCAACAACCCGTTACCNNNTTGTCGGGTTGTACCGTTCCAgtttaac > 3:154620/1‑268 (MQ=255) ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACCNTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGAACCGCTTTCAGGCCGTCAACATTNATGGTTTTATCCCCCAACAACCAGTTAAGGNNTTGCTGGGTGATACCGTTCCAGGTgaaaa > 3:75330/1‑272 (MQ=255) tccgttccgACCTTTTTCTCACGTAGCCAAATTTGCACCGT‑AGTAACGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCCCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGATGACGCCGCCCATCTACAGCAGAAGCAATGAGATTAGACCCGGTACCAGACGTATGACCCGTTTCCAGGCCGGCAACATTCCTTGGTTTCGCCCCAACCAACCCATCACGGTTTTGTTGGGTGTAACCGTTCCggggtatttt > 4:477935/1‑268 (MQ=255) gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCacaaca < 2:143571/229‑1 (MQ=255) aCCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGTAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct < 4:203859/271‑1 (MQ=255) tttttCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt < 8:730346/65‑1 (MQ=255) tttttCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt > 7:730346/1‑65 (MQ=255) tttttCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg > 6:614330/1‑67 (MQ=255) tttttCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg < 5:614330/67‑1 (MQ=255) ttttCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGtttt > 6:629184/1‑39 (MQ=255) ttttCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGtttt < 5:629184/39‑1 (MQ=255) cACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc > 7:747091/1‑75 (MQ=255) cACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc < 8:747091/75‑1 (MQ=255) cACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCa > 7:765353/1‑107 (MQ=255) cACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCATGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCa < 8:765353/107‑1 (MQ=255) aCGGTGCAAATTTTGCAGCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt > 5:326637/1‑78 (MQ=255) aCGGTGCAAATTTTGCACCGT‑GGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTg < 6:898332/120‑1 (MQ=255) aCGGTGCAAATTTTGCACCGT‑GGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTg > 5:898332/1‑120 (MQ=255) aCGGTGCAAATTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACg > 7:771724/1‑72 (MQ=255) aCGGTGCAAATTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt < 6:326637/78‑1 (MQ=255) ggTGCAAATTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATc < 7:1128060/124‑1 (MQ=255) ggTGCAAATTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATc > 8:1128060/1‑124 (MQ=255) aaaaTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc < 5:109888/86‑1 (MQ=255) aaaaTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc > 6:109888/1‑86 (MQ=255) aaaTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc > 7:124117/1‑92 (MQ=255) aaaTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc < 8:124117/92‑1 (MQ=255) aaaTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTa < 7:10705/120‑1 (MQ=255) aaaTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTa > 8:10705/1‑120 (MQ=255) aTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACt < 6:43116/93‑1 (MQ=255) aTTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACt > 5:43116/1‑93 (MQ=255) | ATGCTGCCTTCCCCGACAGATTCCAGGGTA‑ACCAGCGGCCAGTGCGCCACCTGTTTATCACGGTCGTAAAGTTCAATTTCCCCTACCCGCTGATGGGCGCTAATTGGCGCGGTGAGTTCTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGT‑AGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCT > NC_000913/2081421‑2081937 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |