Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,255,992 A→T 6.3% K213I (AAA→ATA) ‡ yeiL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,255,9920AT 6.3% 224.5 / 5.6 63K213I (AAA→ATA) ‡yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (35/24);  new base T (2/2);  total (37/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

TTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAA  >  NC_000913/2255742‑2256264
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                  
ttAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGCTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGCGATGGACCCGGAGAATAAATTCTCCGGGTGGATGCAGTaaaa                                                                                                                                                                                                                                                            >  2:291499/1‑275 (MQ=255)
                               aTCGCCTGGAAGCATTTATATGTCTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAATATTTAGGCGATTCTTATCGACATCTTTTATATGTTCTCTCGCAGTTCATTCACTACGGTTTATTAATTAAAAGCAAGAAAGGGTAACTCATTAAAAACAGAAAGCAGTTGCCAGGACTGGCGCTGGATATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTACAATAGCGCGATTGCCGGATGCa                                                                                                                                                                                                                            <  4:12978/275‑1 (MQ=255)
                                tCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGCTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCaa                                                                                                                                                                                                                           >  2:131525/1‑275 (MQ=255)
                                      ggCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTCAATAGCGCGATTGCCGGATGCAACGCTTaa                                                                                                                                                                                                                    >  4:286572/1‑275 (MQ=255)
                                                  atTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                                                                                                                                                                                                        <  2:286573/275‑1 (MQ=255)
                                                      ctctCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGt                                                                                                                                                                                                     >  1:44135/1‑275 (MQ=255)
                                                           gCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTAc                                                                                                                                                                                                 >  2:108721/1‑274 (MQ=255)
                                                                           tATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc                                                                                                                                                                                 <  4:108720/274‑1 (MQ=255)
                                                                             gcACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGAATCTCATTAAAAACAGAAAGCAGTTNTCAGGACTGGGGCTGGAGATTGCACCGGAGAATAAAt                                                                                                                                                                                                                                                                                  >  3:110649/2‑176 (MQ=255)
                                                                                 gAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTGCTCGCGCAGTTCATTCACGACGGTTTATTTATTACAAGGAAGAAAGGGAATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGCGAATAAATtctc                                                                                                                                                                                                                                                                              <  1:110649/176‑1 (MQ=255)
                                                                                                 cGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTAAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTAAAAGTCGACCATTACGCCCGACCATTAAAAAACCCGGCaa                                                                                                                                                          >  4:94903/1‑275 (MQ=255)
                                                                                                                        tATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGc                                                                                                                                                                                                                                                                   >  5:803534/1‑148 (MQ=255)
                                                                                                                                tCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAt                                                                                                                                                                                                                                                                                  >  5:765212/1‑125 (MQ=255)
                                                                                                                                tCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAt                                                                                                                                                                                                                                                                                  <  6:765212/125‑1 (MQ=255)
                                                                                                                                           ttCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt                                                                                                                                                                                                            >  4:563406/1‑183 (MQ=255)
                                                                                                                                            tctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGcc                                                                                                                                                                                >  2:141219/1‑210 (MQ=255)
                                                                                                                                            tctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAACGCGTTGCTGCTTCCAg                                                                                                                  >  2:523314/1‑272 (MQ=255)
                                                                                                                                            tctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAg                                                                                                                  >  1:90274/1‑272 (MQ=255)
                                                                                                                                             ctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCACTAGCGCGATTGCCGGATGCAACGCTTACTACGTTTTATCCGGTCATCAAATCGAGCATTACGCCAGACCAATAAAGAACCAGGCACTAGTCGCACGAATCCAGTTGGAAAGCGTttctgccaccaacacc                                                                                                              >  4:42406/1‑265 (MQ=255)
                                                                                                                                               cgcgCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                                                                                                                                                                                                        <  2:563407/183‑1 (MQ=255)
                                                                                                                                                gcgcAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGAc                                                                                                                                                                            <  4:141218/210‑1 (MQ=255)
                                                                                                                                                         attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCgg                                                                                                                                                                                                                                 <  6:803534/148‑1 (MQ=255)
                                                                                                                                                          ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                                                                                                                                                                                                    >  5:93070/1‑97 (MQ=255)
                                                                                                                                                          ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                                                                                                                                                                                                    <  6:93070/97‑1 (MQ=255)
                                                                                                                                                          ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtctc                                                                                                                                                                                                                                                                              >  7:144747/1‑103 (MQ=255)
                                                                                                                                                          ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtctc                                                                                                                                                                                                                                                                              <  8:144747/103‑1 (MQ=255)
                                                                                                                                                           tcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                                                                                                                                                                                               >  6:590002/1‑148 (MQ=255)
                                                                                                                                                                acgGTTTATTAATTAAAGGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGa                                                                                           >  4:320921/1‑275 (MQ=255)
                                                                                                                                                                acgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCggg                                                                                                                                                                                                                                                                          >  6:184438/1‑101 (MQ=255)
                                                                                                                                                                acgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCggg                                                                                                                                                                                                                                                                          <  5:184438/101‑1 (MQ=255)
                                                                                                                                                                acgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCTTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGAGCGa                                                                                           >  2:233911/1‑275 (MQ=255)
                                                                                                                                                                acgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGgaga                                                                                           >  4:50475/1‑275 (MQ=255)
                                                                                                                                                                   gTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt                                                                                                                                                                                                                       >  5:256574/1‑148 (MQ=255)
                                                                                                                                                                        ttaattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgg                                                                                                                                                                                                                                                                           <  8:1065987/92‑1 (MQ=255)
                                                                                                                                                                        ttaattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgg                                                                                                                                                                                                                                                                           >  7:1065987/1‑92 (MQ=255)
                                                                                                                                                                           attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                                                                                                                                               <  5:590002/148‑1 (MQ=255)
                                                                                                                                                                              aaaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAtt                                                                                                                                                                                     >  2:293377/1‑171 (MQ=255)
                                                                                                                                                                                  gCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc                                                                                                                                                                                 <  4:293376/171‑1 (MQ=255)
                                                                                                                                                                                   cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                                                                                                                                                                                                    >  6:510013/1‑72 (MQ=255)
                                                                                                                                                                                   cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                                                                                                                                                                                                    <  5:510013/72‑1 (MQ=255)
                                                                                                                                                                                   cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatg                                                                                                                                                                                                                                                                       <  7:502515/85‑1 (MQ=255)
                                                                                                                                                                                   cAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatg                                                                                                                                                                                                                                                                       >  8:502515/1‑85 (MQ=255)
                                                                                                                                                                                     aGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                                                                                                                                                       <  6:131020/98‑1 (MQ=255)
                                                                                                                                                                                     aGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                                                                                                                                                       >  5:131020/1‑98 (MQ=255)
                                                                                                                                                                                             tATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCTTTTTGGCGAAATAGc                                                              >  2:415557/1‑274 (MQ=255)
                                                                                                                                                                                              aTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCg                                                            >  1:89404/1‑275 (MQ=255)
                                                                                                                                                                                               tctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg                                                                                                                                                                                           <  6:256574/148‑1 (MQ=255)
                                                                                                                                                                                                ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatg                                                                                                                                                                                                                                                                       <  5:819242/72‑1 (MQ=255)
                                                                                                                                                                                                ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgatg                                                                                                                                                                                                                                                                       >  6:819242/1‑72 (MQ=255)
                                                                                                                                                                                                ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCCCACTCATCAGGTTGGAAAGCGTTGCTTCCGCCAGCGCCCGAAAACCAAGCTGGGGGATTTCCGGCGGCGCTTTTGGCGaaaaaccagaa                                                          >  4:448002/1‑267 (MQ=255)
                                                                                                                                                                                                                  cAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                                                                                                                                               <  8:65936/109‑1 (MQ=255)
                                                                                                                                                                                                                  cAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                                                                                                                                               >  7:65936/1‑109 (MQ=255)
                                                                                                                                                                                                                    gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatt                                                                                                                                                                                                                                                        >  5:874177/1‑66 (MQ=255)
                                                                                                                                                                                                                    gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatt                                                                                                                                                                                                                                                        <  6:874177/66‑1 (MQ=255)
                                                                                                                                                                                                                      tggcAGGACTGGCGCTGAGGATGGACCCGGAGAATAAATTCTGCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTGTTAACCGGTTTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCAGCAATAGTCGCACTCATCAGGTTGGAGAGCGTTGCTGCTGCCAGCGCCCGTAAACAAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGGCAACGACAACACCGa                                    <  1:72053/272‑1 (MQ=255)
                                                                                                                                                                                                                      tGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGa                                    >  2:221344/1‑275 (MQ=255)
                                                                                                                                                                                                                           ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                                                                                                                                                                                                <  7:210811/83‑1 (MQ=255)
                                                                                                                                                                                                                           ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                                                                                                                                                                                                >  8:210811/1‑83 (MQ=255)
                                                                                                                                                                                                                             aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                                                                                                                                                                                                >  7:47287/1‑81 (MQ=255)
                                                                                                                                                                                                                             aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                                                                                                                                                                                                <  8:47287/81‑1 (MQ=255)
                                                                                                                                                                                                                                      ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGt                                                                                                                                                      >  5:66624/1‑146 (MQ=255)
                                                                                                                                                                                                                                      ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGt                                                                                                                                                      <  6:66624/146‑1 (MQ=255)
                                                                                                                                                                                                                                      ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAANTGGGCGATTTCCGGCGCGCGGTTTGGCGAAATAGCCGAAAAGGCACAAACGACAACAACGAAAGAACAAAAGGTAg                     >  3:128406/1‑274 (MQ=255)
                                                                                                                                                                                                                                                cgggAAAATAAATTCTCCGGAATACTGANTAAAAATTTATTTTCAATAGCGCGTNTGCCGAATGCAACGCTTAATACGTTTTACCCGGTCTACAAATCGAGCATTACTCCAGACCAATAAAGACCCCGTCAATAGNCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACcaca           <  3:89404/272‑1 (MQ=37)
                                                                                                                                                                                                                                                ccGGGGAAGAAAATCTCCGGGAGAAGTCAGTAAAAATTTTTTTCAATAGCGCGGTTGCGGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCATGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGGTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACcaca           <  4:291498/274‑1 (MQ=37)
                                                                                                                                                                                                                                                ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg                                                                                                                                                                                           >  6:368421/1‑99 (MQ=255)
                                                                                                                                                                                                                                                ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg                                                                                                                                                                                           <  5:368421/99‑1 (MQ=255)
                                                                                                                                                                                                                                                       agaaaTTCTCCGGGATGATGCATTAAAAATTATTTGCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTAATCCGGTCGACAAATCGAGCATTAAGCCAGACCAATAAAGAACCCGGGAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCa    <  4:221343/272‑1 (MQ=255)
                                                                                                                                                                                                                                                        aaaaTTTTCAGGGAGGAGGAAGAAAACANTNTTTTCAATAGAGCGATTGCCGGATGCAACGCTTAATATGTTTGATNCGGTCTACAAATCGAGAAGTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGANAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCaaa  <  3:44135/274‑1 (MQ=37)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                  
TTAACTCAGAATCAATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACGCAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCGTTGCTGCTGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCGCGTTTTGGCGAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAA  >  NC_000913/2255742‑2256264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: