Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,318,693 | G→T | 5.2% | T395K (ACG→AAG) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,318,693 | 0 | G | T | 5.2% | 584.1 / 14.1 | 166 | T395K (ACG→AAG) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
Reads supporting (aligned to +/- strand): ref base G (76/81); new base T (5/4); total (81/85) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01 |
Only 100 of 170 total aligned reads displayed.
ATACAGCGGCGTTCGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACC > NC_000913/2318419‑2318956 | tACAGCGGCGTTCGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCg < 2:251930/274‑1 (MQ=255) cgccgcgTGCGCAAGTCATGTATGACGGTGGCAAGGACCCTCGATTGTGACTGCCTCGCCTGTTCCGCTGCGTGTGCCCTCCCCTGCAACGACTCATCCATCTTCACGCGCGAAGAAACCACCACCAGCACACAATTGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAg < 4:123191/271‑1 (MQ=255) gcggcgTTAGCAGCTCATTCCTGACGGTGGCAAGCAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCATCACGCGCGAAGCAACATCCACCCGTACACAAATTGCCACGTGTTAATTACGATAGCGCGAATAGACTAAGCTCATTCGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGGCCACAGATAATTTGCGTCGGTCGTTGGCGGTCCTCATGCGTAAGc < 4:384750/275‑1 (MQ=255) cggcgTTCGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGTTTGTGCCATCTCCTGCAACGACTCTTCCTTCTTCACGCACCAAGAAACCTCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGTCGATGCTGATTTGCAGATCGCTATTGTTGCTGGTCAGGACATTAACAAAATTGAACTGCTGCCCACAGACAATTGGCGGAAGTAGTTGGAGGTCCCCATGCTTAAGCa > 4:191361/1‑275 (MQ=255) ggcgTTCGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCat < 2:263769/275‑1 (MQ=255) gcgTTCGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCCCACGCGTAAGCat > 1:126538/1‑274 (MQ=255) cgTTGGCAGCTCATGCCTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCatat < 4:346249/275‑1 (MQ=255) ccttCGCAGCTCATGACTGACGGTGTCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCatat < 2:126398/273‑1 (MQ=255) cGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTgag > 2:524121/1‑275 (MQ=255) cagcaaagaactgacGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑t < 2:125376/266‑1 (MQ=255) acGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGACTGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATTCGTCAGCCTATTGAGA‑TCGGTATgcgcacg > 1:5765/1‑272 (MQ=255) tGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGATGCTTGTGCCATCGCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCg < 2:278962/275‑1 (MQ=255) atgcggttttTACCGGCTCGCCTGTGCCGCTGCTGTGGCCCTCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACCAATGGCCACGTTTTCATTACGATAGAGCGAATGGACGAAGCTGATTTGAAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTaaa < 4:332456/268‑1 (MQ=255) tGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCTACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACCCAAAATGCAAATACCCACTgc > 4:15350/1‑271 (MQ=255) tGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTgaga < 2:109498/201‑1 (MQ=255) gCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGGTTTGCAAATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGcgcg < 2:372430/275‑1 (MQ=255) aCTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTTACGCCATCAGCGGAACGCAAAATGCAAATACCCAGTGGCCCGGAggac > 4:529995/1‑273 (MQ=255) tctTCCATCTTCACGCCCGAAGAAACCTCCACCCGCCCCCAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTTATTTGCAGATTGGTATTGTGGCTGGTAAGGACATCAACAAAATTGACCTGCTGCCCACAAATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGAc < 4:139025/275‑1 (MQ=255) tCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCtt > 4:475924/1‑274 (MQ=255) aTCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAATCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGtt < 2:33309/275‑1 (MQ=255) aTCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATTATTTGCATCATACGTTGGCGGTCCTCATGCGTAAGCATATTGATA‑TAGGTATTCCCCAGTTCGTTACTTAAACTATTCCCGCCATCAGCGGTACGCACATTGCCGAAACCCACCGGCCAGGGAGCGGCAATTTTGCGATTAACCTGCCCAttct > 4:428202/1‑272 (MQ=255) ccaccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCAAATTGAGA‑TAGGTATGCCCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCAACTGGCCGGGACGCAACAATCTGGCGATTGAAATGCCCATTttc > 4:529627/1‑274 (MQ=255) accaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCg > 6:554112/1‑148 (MQ=255) aGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGt > 7:6698/1‑146 (MQ=255) cacacaAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGTCGCGGATGCGACAATCTTGCGATTGAACTGCTTATGTTCTTCCAGTc > 2:514866/1‑275 (MQ=255) ccACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑Ta > 6:674946/1‑148 (MQ=255) aCGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGc > 7:7613/1‑135 (MQ=255) aCGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTgag > 5:95145/1‑143 (MQ=255) ttttCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAg > 8:47403/1‑131 (MQ=255) ttACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCTTACGCat < 6:517479/128‑1 (MQ=255) tACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTa < 5:388291/122‑1 (MQ=255) tACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTa > 6:388291/1‑122 (MQ=255) tACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑Ta < 8:708485/136‑1 (MQ=255) tACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAg > 8:495448/1‑148 (MQ=255) cgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTa > 5:265001/1‑130 (MQ=255) cgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTa < 6:265001/130‑1 (MQ=255) gAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACg < 2:475925/275‑1 (MQ=255) aaTGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑t > 5:546466/1‑123 (MQ=255) aTGGACGAAGCTGATGTTCGGATTGGTATGGTTGCTGGTCAGTACATCAACAAAATGGCCCTGCTGCCCCCAGATAATTTGCGGCAGTCGTTTGCGGGCATCATGCGTAAGCATATTGAGA‑TAGTGATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTc < 4:207857/275‑1 (MQ=255) cGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCTTACGCatg > 7:1036694/1‑109 (MQ=255) aaGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGa < 7:495448/148‑1 (MQ=255) gATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCa < 8:937962/101‑1 (MQ=255) ttGCAGATTGGGATTGTTGGTGGTCACGCCCTCAACAAAGTTTACCTGCTGCCCAATAATCACTTTCGTCAGGAGTTGGCAGTCCTCATGCGTAAGCATATT‑AGAGTAGGGATGCTCCAGTTCGTTACTTAAGAGATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCACGGAGGCGACAAGCTTGCGATTGAACTGATCATGTTCTTCCAGTCGCAGGGCGTCGCATTCCGCAGGAATGAAAATACGTCGCGCGT‑ACATCCGTg < 4:125252/275‑1 (MQ=255) cAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCTCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAAATGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCga > 4:219214/1‑275 (MQ=255) gATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTaaa < 8:535211/128‑1 (MQ=255) gATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTaaa > 7:535211/1‑128 (MQ=255) ttGGGATTGTTGCTGGTCAGGACATCAACAAAAGTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGt < 2:529996/275‑1 (MQ=255) tGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGT‑TAGGTATGCGCCAGTTCGtt > 6:83740/1‑118 (MQ=255) tATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTaaa < 7:1065556/122‑1 (MQ=255) tATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTaaa > 8:1065556/1‑122 (MQ=255) ttgttgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTa > 8:826463/1‑118 (MQ=255) ttgttgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCaaaa < 5:674946/148‑1 (MQ=255) ttgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCTTACGCatga < 5:945490/85‑3 (MQ=255) ttgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCTTACGCatga > 6:945490/1‑83 (MQ=255) tgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCTTACGCatga < 7:427202/84‑3 (MQ=255) tgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGcc > 6:986806/1‑147 (MQ=255) tgCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCa < 5:986806/148‑1 (MQ=255) gCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTTCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTAATGTTCTTACAGTCGCAGGGCGTCGCTTTCCCCCGGAATGAAAATCCGTCGCCCGT‑ACATCCGTTCGGGAGAAAACAATgaaa > 4:161740/1‑272 (MQ=255) gCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAg > 2:201677/1‑275 (MQ=255) cTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTa < 7:241396/135‑1 (MQ=255) cTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTa > 8:241396/1‑135 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATgcgca < 8:1023351/97‑2 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGc < 5:227397/143‑1 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGc > 6:227397/1‑143 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATa > 6:131473/1‑148 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATa > 8:774222/1‑148 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATAGAACTGCTCATGTTCTTCCAGTCGCAGGGCGGCGCTTTCCGCCGGAATGAAAATACGTCCCACGG‑ACATCCGAGCGGGAGTAAACCATGAAGccc > 4:116492/1‑273 (MQ=255) ggTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGAAGCCATCAGCGGTACGCAAAATGCAGATCCCCACTGGCGCGGAGGCGAACATCTTTCGTTTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCCTTCCGCCGGAATGGAAATAAGGCCCGCGTAACACCGGCGCGAGAG‑AAAAAATGCAGccc > 4:458823/1‑273 (MQ=255) tCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGc > 7:537493/1‑141 (MQ=255) cAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACt < 5:799324/105‑1 (MQ=255) cAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGc < 5:204291/140‑1 (MQ=255) cAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGc > 6:204291/1‑140 (MQ=255) ggACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑Ta > 8:544460/1‑82 (MQ=255) aTCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTAATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGtt > 5:234444/1‑96 (MQ=255) caacaaAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATa < 5:488982/137‑1 (MQ=255) tacaaAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGcaca < 2:15350/273‑1 (MQ=255) acaaAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTg < 8:792802/69‑1 (MQ=255) acaaAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTg > 7:792802/1‑69 (MQ=255) acaaAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCg > 6:714198/1‑90 (MQ=255) caaAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATg < 6:972613/79‑1 (MQ=255) aaaaTTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAg < 7:1023107/148‑1 (MQ=255) aaTTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGc > 2:397597/1‑275 (MQ=255) aTTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGcc > 7:819796/1‑79 (MQ=255) aTTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGc > 7:9747/1‑125 (MQ=255) aCCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAAt < 2:219215/275‑1 (MQ=255) aCCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAAt > 2:422412/1‑275 (MQ=255) cacaGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGt < 7:257071/72‑1 (MQ=255) cacaGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGt > 8:257071/1‑72 (MQ=255) acaGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTg > 6:886470/1‑142 (MQ=255) acaGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTg < 5:886470/142‑1 (MQ=255) caGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTaa > 8:1003310/1‑40 (MQ=255) caGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCg > 8:350196/1‑132 (MQ=255) caGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCg < 7:350196/132‑1 (MQ=255) caGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCCGCCCCGGCAAGCCCATCCAGCAAAATTGCGTTAAGGAt > 4:284213/1‑275 (MQ=255) aGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTa < 7:93392/98‑1 (MQ=255) gCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGc < 6:262035/132‑1 (MQ=255) cAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCa > 5:708484/1‑104 (MQ=255) gTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAg < 5:1026817/113‑1 (MQ=255) tgggtcccctggagTAGGCATATTGGGA‑TAGGTATGCGGCAGTTCGTTACTTAAAATATTGAGGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCa < 4:343934/262‑1 (MQ=255) ccTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGc > 8:602454/1‑148 (MQ=255) | ATACAGCGGCGTTCGCAGCTCATGACTGACGGTGGCAAGGAACATCGATTTTGACTGGCTCGCCTGTTCCGCTGCTTGTGCCATCTCCTGCAACGACTCTTCCATCTTCACGCGCGAAGAAACATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGA‑TAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGT‑ACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACC > NC_000913/2318419‑2318956 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |