Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,457,128 | T→A | 5.2% | M678L (ATG→TTG) | fadJ ← | enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,457,128 | 0 | T | A | 5.2% | 458.3 / 8.5 | 128 | M678L (ATG→TTG) | fadJ | enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase |
Reads supporting (aligned to +/- strand): ref base T (59/62); new base A (4/3); total (63/65) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01 |
Only 100 of 135 total aligned reads displayed.
ACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTC > NC_000913/2456861‑2457396 | aCCAGAGAAAGAAAACGGTGGCCGGCATTTTGCCTTATCCCTTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCa < 4:428845/275‑1 (MQ=255) aCGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACCTTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAATTCGTTTCATTATTGCAACCACTTCGACcgc > 4:392321/1‑275 (MQ=255) ccGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCACTATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgaga > 2:117304/1‑275 (MQ=255) ggCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgagaga < 2:518023/275‑1 (MQ=255) tgtgCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa < 2:102914/275‑1 (MQ=255) tgCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGACCCACACTCCGTGGCAAGCAGTTGCATTATTGCAACCACTACGCCCGCGCCGAGAGAATCGat > 4:470218/1‑274 (MQ=255) tCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGt > 2:545199/1‑275 (MQ=255) tCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGt < 2:299504/275‑1 (MQ=255) aaTGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCAATCTCGACCAAACGCTCGCAAGGGGTAATACGGGAACCATACTGCCTGCCAAGTCGTTGCCTTATTCCAACCACTTCGCCCGCCCACAAGGAATCGAAATAGCGAAACGGTCCAcag > 4:20751/1‑273 (MQ=255) agagacaggTCAATATGAGTTTACATTTTTACAAACAATCCGCCATTCAGCGCGGATTCATATAGCTTTGACCGTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCACAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAcc < 4:223079/267‑1 (MQ=255) gTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACAAAACGCTCGCAAGTGGTAAAACGGGAACCATACGGCGTGGCAAGTCGTTGCATAATTGCAACCACTTCGCACGCGCCGAGAAAATAGACTTACAGGAACGCACCACCAACAAAGGGGGAAAAACCAAAGGCaaaaaacacc > 4:514398/1‑268 (MQ=255) tttACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCaaa > 2:223080/1‑252 (MQ=255) tACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCAGGCAAATCCATTTCACGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCACAATAGCGAGCCCATAAccccaccact > 4:268046/1‑268 (MQ=255) cATTTTGCCCAACACACCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCGTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTAGTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca < 2:494105/274‑1 (MQ=255) ttttGCCAAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCacgcacgc < 2:353559/275‑1 (MQ=255) tgtGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGGCAGTTGCAGTTGTGTTCCAAAAACTTTCCCCACGCGCGCCCAACTCGACCAAACGCTCGCAAGGGGTAAAACGCGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGGGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCt < 2:471879/273‑1 (MQ=255) acacTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa < 1:3234/229‑1 (MQ=255) cacTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGGCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGAGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCgcgc > 2:139185/1‑178 (MQ=255) ccATTCAGCGCGGATTCATATAGCTTTGACCTTCGTATGGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCtca < 2:268047/275‑1 (MQ=255) cATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCtcat < 2:459715/275‑1 (MQ=255) cAGCGCGGATTCATATAGCTTTGACCTTCTTAATGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCGCCACGCGCGCCCATCTCGAACAAACGCTCGCAAGGGGTAAAACCGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCAGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAac < 2:514399/275‑1 (MQ=255) cgGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAACACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCCCCGAGAGAATCCATATACCGGAACGGTCAACCCAGAAATGGCGGAAAACCAATGCCAAATCCCGCGCCACTACCCCCGTTCCGCCACCTACCGAAAACCTGCCAATCAacctca > 4:377058/1‑270 (MQ=255) aTTCATATAGCTTTGACCTTCTGATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTa < 2:392322/275‑1 (MQ=255) ttCTNNTAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAANTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa < 4:545198/207‑1 (MQ=255) gCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCattat < 7:1040896/129‑1 (MQ=255) ttGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCAATAATGCaa < 6:631165/131‑1 (MQ=255) ttGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCAATAATGCaa > 5:631165/1‑131 (MQ=255) ttcttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACtt > 6:769027/1‑131 (MQ=255) ttcttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACtt < 5:769027/131‑1 (MQ=255) cttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATAACCCCGTCACCCACGCTACGGATAACCCGCTCCACAACACCACATACTACTTCATTCCGCATaaa > 4:263855/1‑274 (MQ=255) tGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCa < 5:21249/121‑1 (MQ=255) tGCAGGTCAGTTGCAGTTGTGTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCa > 6:21249/1‑121 (MQ=255) ggTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTc < 7:694296/121‑1 (MQ=255) ggTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTc > 8:694296/1‑121 (MQ=255) gttgcagttgTTTTCCAAAGACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGc < 6:480774/107‑1 (MQ=255) gttgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGc > 5:480774/1‑107 (MQ=255) tgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCattat < 7:637315/102‑1 (MQ=255) tgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCattat > 8:637315/1‑102 (MQ=255) tgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCg < 6:1042190/133‑1 (MQ=255) tgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGt > 8:1019655/1‑148 (MQ=255) tgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa > 5:460593/1‑128 (MQ=255) tgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa < 6:460593/128‑1 (MQ=255) tgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCg > 8:879829/1‑148 (MQ=255) ttttCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCgag > 6:840297/1‑148 (MQ=255) aaaaaCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATgg > 6:676236/1‑148 (MQ=255) aaaaaCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATgg < 7:879829/148‑1 (MQ=255) aaaCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGaaa < 7:317306/143‑1 (MQ=255) aaaCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGaaa > 8:317306/1‑143 (MQ=255) cTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGaa < 7:1019655/148‑1 (MQ=255) tttCCCCACGCGCGCACATCTCGACCAAACGCGCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAtt < 1:100304/275‑1 (MQ=255) gttCCCCACGCGAGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACAGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAt < 1:73038/273‑1 (MQ=255) cccACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCg < 7:681626/109‑1 (MQ=255) cccACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCg > 8:681626/1‑109 (MQ=255) cgcgcgCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGc < 5:676236/148‑1 (MQ=255) cgcgcgCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACCCAACGTACTGCTTCATTCAGCATCAACATCCCACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTg > 2:427509/1‑275 (MQ=255) cccATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATa > 8:401974/1‑148 (MQ=255) cATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCgag < 8:721417/120‑1 (MQ=255) cATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCgag > 7:721417/1‑120 (MQ=255) cATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCCTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGACAGAATCGATATAGCGGAACGTTCCACCGAGAAATGGCGTAAAACCAATGCCAAATAcg > 6:216447/1‑147 (MQ=255) ctcGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGa < 6:155333/104‑1 (MQ=255) ctcGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGa > 5:155333/1‑104 (MQ=255) cGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGcc > 6:1071141/1‑148 (MQ=255) aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCAc > 8:178612/1‑109 (MQ=255) aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCAc < 7:178612/109‑1 (MQ=255) cAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAata < 7:401974/148‑1 (MQ=255) aaaCGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCaa < 7:313235/133‑1 (MQ=255) aCGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCgag > 6:370905/1‑108 (MQ=255) aCGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATcc < 5:1071141/148‑1 (MQ=255) aaGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa < 5:217423/116‑1 (MQ=255) tAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCt > 5:832010/1‑148 (MQ=255) aaaCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTAc < 6:832010/148‑1 (MQ=255) aaCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGc < 6:680728/111‑1 (MQ=255) aaCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGc > 5:680728/1‑111 (MQ=255) aCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACgg > 6:536179/1‑148 (MQ=255) cGGGAACCATCCTGCGTGGCAAGTCGTTTCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGAc < 2:88088/275‑1 (MQ=255) cGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGa < 5:506759/148‑1 (MQ=255) cGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGa > 6:506759/1‑148 (MQ=255) gggAACCATACTGCGTGGCAAGTCGGTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGAGATAGAGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGAc < 4:171013/274‑1 (MQ=255) ggAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATa < 5:536179/148‑1 (MQ=255) gAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCgag > 3:79909/1‑86 (MQ=255) gAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTCAATGGCGGGACCgcact > 1:102041/1‑270 (MQ=255) gAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAAAACAACGCACTGCTTCATTCAGCATCAACATCCCACANCGTTCAGCAACCGTCGGTGCGGATATTCGCCCCTGCCTCTGTCGTCCAATCAGCGCGTAACTGGCggggaagtcct > 3:127758/1‑266 (MQ=255) ccATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca > 7:83545/1‑133 (MQ=255) ccATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca < 8:83545/133‑1 (MQ=255) ccATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCt > 5:398444/1‑148 (MQ=255) cATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTg < 5:216447/148‑1 (MQ=255) cATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcattc < 1:41304/176‑1 (MQ=255) aTACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGACTCGATATAGCGGAACGGTCCACCGAGAAATGGCGGaaaa < 8:663060/94‑1 (MQ=255) ttcagggTGGCAAGTGGTTGCAATATTGCGAACGCTGCGCCCGCGCCAAGAGATTCGATATGGCGGAACGGACCACGGAGAAAGGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGttttt < 4:269915/268‑1 (MQ=255) tGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCtttt > 2:349565/1‑274 (MQ=255) gCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGaa > 7:444495/1‑59 (MQ=255) gCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAAcaca < 2:335050/149‑1 (MQ=255) tggTTGCAATAATGCAACGACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATg < 8:265186/69‑1 (MQ=38) tggTTGCAATAATGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGt > 8:135299/3‑148 (MQ=255) tCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCt > 6:595322/1‑134 (MQ=255) tCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGAATAACCTGCt < 5:595322/134‑1 (MQ=255) cGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAAc > 8:41214/1‑145 (MQ=255) cGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTa < 5:840297/148‑1 (MQ=255) gTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTc < 1:10441/275‑1 (MQ=255) ttGCAATAATGCAACGACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACt > 8:656344/1‑148 (MQ=255) | ACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTC > NC_000913/2456861‑2457396 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |