Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,519,225 | T→A | 5.8% | intergenic (+20/+32) | yfeD → / ← gltX | DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,519,225 | 0 | T | A | 5.8% | 366.1 / 10.9 | 105 | intergenic (+20/+32) | yfeD/gltX | DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase |
Reads supporting (aligned to +/- strand): ref base T (52/45); new base A (3/3); total (56/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-02 |
Only 100 of 108 total aligned reads displayed.
GTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAG > NC_000913/2518963‑2519490 | gTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAACACGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCCAGATATTGACGAATCAGCATAAATAAACACCGGCAGGAAATTATCTCCTTCCg > 1:12567/1‑274 (MQ=255) attttGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTGGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa < 2:513371/273‑1 (MQ=255) cGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCt > 1:48007/1‑275 (MQ=255) aaCACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAt > 4:126395/1‑275 (MQ=255) gccacccAAAACACCCCAAAGTTTTTGGANAGTAAAGCGAAAATTGCTGAAAACGNTTCTGGAGCACGAGCCCAGGCCTATAGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGNGCAATTTTTGTAACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt < 3:88772/270‑1 (MQ=255) tCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAg > 2:304612/1‑274 (MQ=255) acCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCTTATCTACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGG‑AAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCATGATATTATCTCCTGTCGTTTATCTTTTTACACGCTAATTACTGCTTATTTTCGCGTTCACCAATAATATcc > 1:93496/1‑274 (MQ=255) tGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATTTTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgag > 2:143099/1‑272 (MQ=255) ttGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtt < 2:126396/275‑1 (MQ=255) cTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACg > 2:112421/1‑275 (MQ=255) gaaaggagaATTGCTGAATAAGGCCCTGGGGCACGCGCCCCGGCGTATCGACAAATCATCAAAGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGGGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCt < 4:304611/270‑1 (MQ=255) aTTGCTGAAAACGCTCCTGGGGAACGCGCCCCGGCGTATCGAAAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTg < 4:112420/274‑1 (MQ=255) tGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc > 4:355274/1‑168 (MQ=255) tGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGc > 2:64172/1‑274 (MQ=255) gCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCt < 2:355275/168‑1 (MQ=255) cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTACCTCGCCC‑GTCTCAATATTGACGAATCAGCATAAATAAAAAACGGCAGGAAATCATCTCCTGCCGTTTATCTTTTTAAACGCTAATTACTACTGATTTTCGCGTTCAGAAATAAAATCTAGCGCTTTGTTGATACGTTCGATCCGGCGGGTCTTACGAATTGCGTAAAAgg > 4:211163/1‑275 (MQ=255) cgcgCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCACGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGaa > 2:90615/1‑272 (MQ=255) gggcCCCGGGGTATCGAAAAATAATAAACGCGATAGATAATATGACAGACATTTAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGt < 4:301023/273‑1 (MQ=255) cgcCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTa > 2:368394/1‑275 (MQ=255) cgcCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTa < 2:9725/275‑1 (MQ=255) cGGCATATCGAAAAATCATCAACGCGATAGATAATATGACAGACAGTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACTCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATc < 1:98263/275‑1 (MQ=255) gggaataaaCAAATCATAAACGCGATAGNTNATATGACAGAAATTGAGAAGGAAAAAGTCGCGCAATTTTTGTCACNTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATNTTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCa < 3:48007/267‑1 (MQ=255) tATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtattat < 7:860089/148‑1 (MQ=255) cGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg > 2:265738/1‑253 (MQ=255) aCAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa > 4:390896/1‑164 (MQ=255) aCAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGAATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGga < 2:10409/275‑1 (MQ=255) aaaTCATAAACGCGGTAGATAATATGACAGACATTGAGAAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga < 2:5148/275‑1 (MQ=255) aaaCCATAAACGCGATAGATAATATGACAGACATTGGTCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGaa < 4:265737/253‑1 (MQ=255) aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtct > 5:26794/1‑142 (MQ=255) aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtct < 6:26794/142‑1 (MQ=255) aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGAc > 2:157416/1‑275 (MQ=255) atcatcAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACg < 2:390897/164‑1 (MQ=255) tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg > 7:524884/1‑125 (MQ=255) tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg > 7:261857/1‑125 (MQ=255) tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg < 8:261857/125‑1 (MQ=255) tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg < 8:524884/125‑1 (MQ=255) acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAACACGGCAGGATATTATCTCCTGCCGTTCATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCCGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTCCGCGAACGGTAACATCCAGTGCTAGAGCCTGCTCCGCCCCGGTTCCGGCGACACGCAg > 2:16507/1‑275 (MQ=255) acaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtt > 4:542597/1‑128 (MQ=255) aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTTCGGGTTTTACCAATTGCGTGAACGGTAAAATCCAGTGCTGGAGACTGCCCCGCACAGGTTACGGCCACACGccac > 2:476078/1‑271 (MQ=255) ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgctg < 7:113881/146‑1 (MQ=255) ttGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgctg > 8:113881/1‑146 (MQ=255) tGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa < 2:542598/128‑1 (MQ=255) gAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCAGACCa > 1:58308/1‑274 (MQ=255) aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTTCGTGAACGGTAACATCCAGTGCTGGAAACTGCCCCGCACCGGTTACGGCGACACGCAGCGCCATACCAACttt > 2:345767/1‑274 (MQ=255) cAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAATGGCAGGAtatta > 8:743654/1‑87 (MQ=255) cAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtatta < 7:743654/87‑1 (MQ=255) cAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc > 6:1017156/1‑138 (MQ=255) cAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc < 5:1017156/138‑1 (MQ=255) cACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt > 8:729758/1‑121 (MQ=255) cACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt < 7:729758/121‑1 (MQ=255) gTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGAGTGAACGGTAACa > 1:137842/1‑195 (MQ=255) aaGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt > 7:1112791/1‑133 (MQ=255) aaGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt < 8:1112791/133‑1 (MQ=255) aaGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt < 6:640008/133‑1 (MQ=255) aaGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt > 5:640008/1‑133 (MQ=255) atgAATTCGCAATTTCCGCGCCC‑GTCTCAATATTGACGAATCAGCATAAATAAAAANCGGCCGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCa < 3:137842/193‑1 (MQ=255) aGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTcc > 7:402432/1‑75 (MQ=255) aGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTcc < 8:402432/75‑1 (MQ=255) gAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCa > 5:365358/1‑133 (MQ=255) gAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCa < 6:365358/133‑1 (MQ=255) aaTTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATccgcc < 4:368393/275‑1 (MQ=255) tttgCAATTTCCCCGCCCTTTCACG‑TATTNNAGAATCAAAATAAATAAAAGACGGCAGGATNTTATCTCCTTCCTTTTATCTTTTTCCACGCTAAGTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTNGTTGCTACGCTCGATCCTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGt < 3:58308/272‑1 (MQ=255) ttCGAAATTTCCTTGCCC‑GTCTAGATATTGACGAATAAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGt < 4:157415/275‑1 (MQ=255) tCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGCCACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCCAATCCGCCCTc > 2:401044/1‑275 (MQ=255) cGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATCCCCACTTCCAGCTCATCCTCCGTCg > 2:259745/1‑275 (MQ=255) tttCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg < 6:21400/143‑1 (MQ=255) tttCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg > 5:21400/1‑143 (MQ=255) tCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt < 6:638430/97‑1 (MQ=255) tCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttt > 5:638430/1‑97 (MQ=255) cGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATcc < 6:391142/116‑1 (MQ=255) cGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATcc > 5:391142/1‑116 (MQ=255) tctcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATATGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGCAGACTACCCCGCACCGGTTACGGCGACACGCAGCGCCATCCCAACTTTACCCATACACACTTCCCGCCCATCCGCCGCCCCCTGAATCGCGtgatg > 2:286098/1‑274 (MQ=255) tctcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCCTCCGCCGTCGCCTGAATAGCGttgtg > 2:8123/1‑270 (MQ=255) tctcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCCGCATACCAACTTTACCCATACCCACTTCCACCCCATCCGCCCTCCCCTGAATAGCGtgcatc > 4:359966/1‑271 (MQ=255) tcgtgATATTGACGAATCAGCAAAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGtgatg < 4:64171/270‑1 (MQ=255) gATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGa < 2:359967/259‑1 (MQ=255) gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGCGCTGGAGACTGCCCCGCACCGGTTACGGCGACCCGCAGCGGCATACCAACTTtaccca > 1:41823/1‑221 (MQ=255) caaaTCAGCATAAATAAAAAAAGTCTAGATATTATCTCCGGCCGTTTAACTTTTTACGCCCTAATTACTGCTGATTTTCGCGTGAAGGAATAAAATCCAGCGCTATGATAATACGCTCGATACTGCGGGTCTAACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACGTCCAGCTCATCCGCAGTCGCCTGAATAGCGTGATGAACGTTTTCAGCg < 4:90614/273‑1 (MQ=255) aagaataaataaAAAACTGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCATCGCTTTGTTGATACGCTCGATACTGCTGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACGTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCca < 2:445118/271‑1 (MQ=255) cATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc > 8:97721/1‑77 (MQ=255) cATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc < 7:97721/77‑1 (MQ=255) ataaataaAAAACGGCAGGATATTATCCCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTTTTGATACGCCCGATACTGCGGTTCTTACCACTTGCGTGAACGGTAACATCAAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGAGGCATTCCAACTTTACCCATCCCCCCTTCCAGCTCATCCGCCGTCGCCTGAACAGCGTAATGAACGTTTTCGGCGGTCcaggc > 4:306149/1‑272 (MQ=255) atggaaagATGTTATCTCCTGTCGTTTATTTTTTTAACCGTTTATTATTACTGTTTTTCGCGTTCAGCAATAAAATCNAACGCTTTGTTGATGCGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGAAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGc < 3:130565/268‑1 (MQ=255) ggcggATGTGATCTCCTGCCGTTTATCATTTTACGGGACAATTACGGCTGATTGTCGTGTTCAGCAATAAAAGCCAGCGCTTTGTTGATACGCTGGATACTGCGGGTCTTACTAATTGCGTGAGCGGTAACATCCAGTGCTGGAGACTTCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAg < 4:143098/272‑1 (MQ=255) gCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg > 7:294411/1‑120 (MQ=255) gCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg < 8:294411/120‑1 (MQ=255) cAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCt > 8:810489/1‑93 (MQ=255) cAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCa < 6:673096/113‑1 (MQ=255) ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa < 6:349907/68‑1 (MQ=255) ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa > 5:349907/1‑68 (MQ=255) ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcc > 6:96860/1‑148 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgt < 7:639641/80‑1 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgt > 8:639641/1‑80 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc > 6:4599/1‑107 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc < 5:4599/107‑1 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg < 8:235813/113‑1 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg > 7:235813/1‑113 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACgg > 6:938391/1‑122 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACgg < 5:938391/122‑1 (MQ=255) atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcccc > 7:490341/1‑148 (MQ=255) | GTCGATACGCAAGTTTGCGAGTTTATTCAGAACACGCCAGCCTTCCATAACACACCAATGTTGATGGAAGCTGAAGAGAGAATTGCTGAATACGCTCCTGGAGCACGCGCCCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCC‑GTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACCGGTTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAG > NC_000913/2518963‑2519490 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |