Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,321 C→T 17.6% A22V (GCT→GTT)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3210CT17.6% 142.2 / 22.3 57A22V (GCT→GTT) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (27/20);  new base T (5/5);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TCCTCTAGAACCGGAATGAATAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGAA  >  NC_000913/2560048‑2560595
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                   
tCCTCTAGAACCGGAATGAATAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgct                                                                                                                                                                                                                                                                                    >  2:499263/1‑275 (MQ=255)
             gaatgaatAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                                                                                                                                                                                                       >  2:57826/1‑275 (MQ=255)
                                                         tttGTATAATTATTTGTTTTTTGTTGTTTAACTATCACCTGATAAGAAACCTTTATTCAATTTGCCGAATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                                                                                                                                                                                                           <  4:352110/275‑1 (MQ=255)
                                                                                           tCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCCTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTTCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgatg                                                                                                                                                                                         <  2:169821/275‑1 (MQ=255)
                                                                                                           cTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                                                                                                                                         <  4:499262/275‑1 (MQ=255)
                                                                                                                     tttGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCACCGGAATGCTGCAACATTCCTGGGCCGGTATGAGATACAAGCTAAATAAGAATTTCTCATGTCTTATATGCTATGAGTAGAACACAAAACAAACAATGATGTTCTTCAATTTTTAAACAAAATTAAc                                                                                                                                                               >  2:353457/1‑275 (MQ=255)
                                                                                                                                              ttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                        >  8:908998/1‑145 (MQ=255)
                                                                                                                                              ttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                        <  7:908998/145‑1 (MQ=255)
                                                                                                                                                ttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                                                                                                                                                                                                   >  8:770544/1‑148 (MQ=255)
                                                                                                                                                                  attccaCTTAATTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaa                                                                                                                                                                                                                                                 >  6:916528/1‑148 (MQ=255)
                                                                                                                                                                        aTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                                                                                                                                                                                                       >  6:1084996/1‑136 (MQ=255)
                                                                                                                                                                        aTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                                                                                                                                                                                                       <  5:1084996/136‑1 (MQ=255)
                                                                                                                                                                         ttAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGATAGCAGTAGAACAAGAAAAAAACAAAGATGNTTATAAATTTTTAAACAAAATTAAAGAGTTCAGGAAAAAAGAACACAAAAACGAAAAAAATAAAAACCAAAAacaca                                                                                                           >  3:117656/1‑275 (MQ=255)
                                                                                                                                                                                               tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                                                                                                                                                                                                       >  7:1047667/1‑97 (MQ=38)
                                                                                                                                                                                               tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                                                                                                                                                                                                       <  8:1047667/97‑1 (MQ=38)
                                                                                                                                                                                                    aGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGatc                                                                                >  2:256336/1‑275 (MQ=255)
                                                                                                                                                                                                         ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGATACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACAAAAGCCAAATAACCAAGAGGaaaagagtcaacac                                                                           >  4:265197/1‑264 (MQ=255)
                                                                                                                                                                                                           aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGt                                                                                                                                                                                                                                >  2:553788/1‑124 (MQ=255)
                                                                                                                                                                                                             aTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                                                                                                                                                      <  5:916528/148‑1 (MQ=255)
                                                                                                                                                                                                               ccATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                                                                                                                                                            <  4:553787/124‑1 (MQ=255)
                                                                                                                                                                                                                cATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAAAACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCaa                                                                   >  2:147001/1‑275 (MQ=255)
                                                                                                                                                                                                                  tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                                                                                                                                                                                                        >  4:144369/1‑125 (MQ=255)
                                                                                                                                                                                                                  tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCaaa                                                                  >  4:214406/1‑274 (MQ=255)
                                                                                                                                                                                                                   gTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAATTACGGAATAAAANACCCAAAAACGAAGAGACTAAACAAGAAGAACAAACACAAAAATATCACAAAGAAAAAGAACAACACA‑AAGAaaaaa                                                                >  3:129162/1‑274 (MQ=255)
                                                                                                                                                                                                                    tttGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca                                                                                                                                                                                               <  7:770544/148‑1 (MQ=255)
                                                                                                                                                                                                                      tGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                                                                                                                                                                                                    <  2:144370/125‑1 (MQ=255)
                                                                                                                                                                                                                                       agTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACAACCGCCAGAAAATCACCAGGAAAACGA‑CAATACGCAGGCAAAACAAGAGGAGCAAACCTGGct                                             >  4:239275/1‑273 (MQ=255)
                                                                                                                                                                                                                                          tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                                                                                                                                         >  8:831115/1‑148 (MQ=255)
                                                                                                                                                                                                                                           cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGCTATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAAACACGAGGAAACAGATCATAACG‑AGGCAAAAAAGCAGGAGAAAGCCTGGCgctgtc                                        >  4:332172/1‑270 (MQ=255)
                                                                                                                                                                                                                                           cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaaaaa                                                                                                                                     >  3:107313/1‑183 (MQ=255)
                                                                                                                                                                                                                                           cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACg                                                                                                         <  1:49080/211‑1 (MQ=255)
                                                                                                                                                                                                                                           cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCa                                        >  2:431074/1‑275 (MQ=255)
                                                                                                                                                                                                                                           cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGAAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCCCCCAAAAACGAAGAGACTAAACACGAAGAACAAACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGAAAAACAACCGGACCACGCCGGGCGTGGCa                                        >  4:355429/1‑275 (MQ=255)
                                                                                                                                                                                                                                             aaaaTAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                        <  5:450481/50‑1 (MQ=17)
                                                                                                                                                                                                                                             aaaaTAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                        >  6:450481/1‑50 (MQ=17)
                                                                                                                                                                                                                                              aaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaa                                                                                                                                                                     <  7:831115/148‑1 (MQ=255)
                                                                                                                                                                                                                                               aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGaa                                                                                                                                 <  1:107313/183‑1 (MQ=255)
                                                                                                                                                                                                                                               aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAg                                                        <  2:332173/255‑1 (MQ=255)
                                                                                                                                                                                                                                                  aGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTgcg                                 <  2:214407/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                                                                                                                                              >  7:565628/1‑64 (MQ=255)
                                                                                                                                                                                                                                                         acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                                                                                                                                              <  8:565628/64‑1 (MQ=255)
                                                                                                                                                                                                                                                          cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGCTCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGACCTACCTgg                         >  2:3248/1‑275 (MQ=255)
                                                                                                                                                                                                                                                              aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                                                                                                                                                                                                  <  7:166711/103‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                                                                                                                                                                                                  >  8:166711/1‑103 (MQ=255)
                                                                                                                                                                                                                                                              aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGa                        >  2:331372/1‑272 (MQ=255)
                                                                                                                                                                                                                                                              aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGa                        <  4:331371/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                                                                                                                                                           <  6:597764/100‑1 (MQ=39)
                                                                                                                                                                                                                                                                        cccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                                                                                                                                                           >  5:597764/1‑100 (MQ=39)
                                                                                                                                                                                                                                                                        cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAttt                                                                                                                                                                              >  6:56568/1‑113 (MQ=255)
                                                                                                                                                                                                                                                                        cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAttt                                                                                                                                                                              <  5:56568/113‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                                                                                                                                                                     >  6:508685/1‑73 (MQ=37)
                                                                                                                                                                                                                                                                         ccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                                                                                                                                                                     <  5:508685/73‑1 (MQ=37)
                                                                                                                                                                                                                                                                         ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                                                                                                                                                      <  6:129535/88‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                                                                                                                                                      >  5:129535/1‑88 (MQ=255)
                                                                                                                                                                                                                                                                         ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTa                                                                                                                                                                 >  6:442276/1‑125 (MQ=255)
                                                                                                                                                                                                                                                                         ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTa                                                                                                                                                                 <  5:442276/125‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCGGTAGaa                                                                                                                                                                                                            >  5:957397/1‑81 (MQ=255)
                                                                                                                                                                                                                                                                          cGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCGGTAGaa                                                                                                                                                                                                            <  6:957397/81‑1 (MQ=255)
                                                                                                                                                                                                                                                                               tgctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTtgatg    <  2:265198/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                               tactgaAACATGCCGGGGGATGGAATAGAAACAAGCTAAAGGAGAACTTCGAATGTCGGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTAGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTtgatg    <  1:117656/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGa                                                                                                                                                             >  1:136913/1‑122 (MQ=255)
                                                                                                                                                                                                                                                                                 ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGaa  >  2:168455/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                   
TCCTCTAGAACCGGAATGAATAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACG‑AGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGAA  >  NC_000913/2560048‑2560595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑