Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,330 G→T 16.1% C25F (TGC→TTC)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3300GT16.1% 213.6 / 23.8 67C25F (TGC→TTC) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (32/24);  new base T (6/5);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

GAATGAATAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGAAGTATTGCC  >  NC_000913/2560061‑2560603
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                  
gaatgaatAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                                                                                                                                                                                                               >  2:57826/1‑275 (MQ=255)
                                            tttGTATAATTATTTGTTTTTTGTTGTTTAACTATCACCTGATAAGAAACCTTTATTCAATTTGCCGAATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                                                                                                                                                                                                                   <  4:352110/275‑1 (MQ=255)
                                                                              tCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCCTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTTCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgatg                                                                                                                                                                                                 <  2:169821/275‑1 (MQ=255)
                                                                                              cTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                                                                                                                                                 <  4:499262/275‑1 (MQ=255)
                                                                                                        tttGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCACCGGAATGCTGCAACATTCCTGGGCCGGTATGAGATACAAGCTAAATAAGAATTTCTCATGTCTTATATGCTATGAGTAGAACACAAAACAAACAATGATGTTCTTCAATTTTTAAACAAAATTAAc                                                                                                                                                                       >  2:353457/1‑275 (MQ=255)
                                                                                                                                 ttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                                <  7:908998/145‑1 (MQ=255)
                                                                                                                                 ttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                                >  8:908998/1‑145 (MQ=255)
                                                                                                                                   ttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                                                                                                                                                                                                           >  8:770544/1‑148 (MQ=255)
                                                                                                                                                     attccaCTTAATTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaa                                                                                                                                                                                                                                                         >  6:916528/1‑148 (MQ=255)
                                                                                                                                                           aTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                                                                                                                                                                                                               >  6:1084996/1‑136 (MQ=255)
                                                                                                                                                           aTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                                                                                                                                                                                                               <  5:1084996/136‑1 (MQ=255)
                                                                                                                                                            ttAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGATAGCAGTAGAACAAGAAAAAAACAAAGATGNTTATAAATTTTTAAACAAAATTAAAGAGTTCAGGAAAAAAGAACACAAAAACGAAAAAAATAAAAACCAAAAacaca                                                                                                                   >  3:117656/1‑275 (MQ=255)
                                                                                                                                                                                  tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                                                                                                                                                                                                               <  8:1047667/97‑1 (MQ=38)
                                                                                                                                                                                  tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                                                                                                                                                                                                               >  7:1047667/1‑97 (MQ=38)
                                                                                                                                                                                       aGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGatc                                                                                        >  2:256336/1‑275 (MQ=255)
                                                                                                                                                                                            ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGATACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACAAAAGCCAAATAACCAAGAGGaaaagagtcaacac                                                                                   >  4:265197/1‑264 (MQ=255)
                                                                                                                                                                                              aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGt                                                                                                                                                                                                                                        >  2:553788/1‑124 (MQ=255)
                                                                                                                                                                                                aTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                                                                                                                                                              <  5:916528/148‑1 (MQ=255)
                                                                                                                                                                                                  ccATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                                                                                                                                                                    <  4:553787/124‑1 (MQ=255)
                                                                                                                                                                                                   cATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAAAACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCaa                                                                           >  2:147001/1‑275 (MQ=255)
                                                                                                                                                                                                     tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                                                                                                                                                                                                                >  4:144369/1‑125 (MQ=255)
                                                                                                                                                                                                     tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCaaa                                                                          >  4:214406/1‑274 (MQ=255)
                                                                                                                                                                                                      gTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAATTACGGAATAAAANACCCAAAAACGAAGAGACTAAACAAGAAGAACAAACACAAAAATATCACAAAGAAAAAGAACAACACAAAGAaaaaa                                                                        >  3:129162/1‑274 (MQ=255)
                                                                                                                                                                                                       tttGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca                                                                                                                                                                                                       <  7:770544/148‑1 (MQ=255)
                                                                                                                                                                                                         tGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                                                                                                                                                                                                            <  2:144370/125‑1 (MQ=255)
                                                                                                                                                                                                                          agTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACAACCGCCAGAAAATCACCAGGAAAACGACAATACGCAGGCAAAACAAGAGGAGCAAACCTGGct                                                     >  4:239275/1‑273 (MQ=255)
                                                                                                                                                                                                                             tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                                                                                                                                                 >  8:831115/1‑148 (MQ=255)
                                                                                                                                                                                                                              cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGCTATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAAACACGAGGAAACAGATCATAACGAGGCAAAAAAGCAGGAGAAAGCCTGGCgctgtc                                                >  4:332172/1‑270 (MQ=255)
                                                                                                                                                                                                                              cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaaaaa                                                                                                                                             >  3:107313/1‑183 (MQ=255)
                                                                                                                                                                                                                              cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACg                                                                                                                 <  1:49080/211‑1 (MQ=255)
                                                                                                                                                                                                                              cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCa                                                >  2:431074/1‑275 (MQ=255)
                                                                                                                                                                                                                              cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGAAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCCCCCAAAAACGAAGAGACTAAACACGAAGAACAAACGCCAGATAATCACGAGGAAACAGATCATCACGAGGAAAAACAACCGGACCACGCCGGGCGTGGCa                                                >  4:355429/1‑275 (MQ=255)
                                                                                                                                                                                                                                aaaaTAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                                <  5:450481/50‑1 (MQ=17)
                                                                                                                                                                                                                                aaaaTAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                                                                                                                                                                >  6:450481/1‑50 (MQ=17)
                                                                                                                                                                                                                                 aaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaa                                                                                                                                                                             <  7:831115/148‑1 (MQ=255)
                                                                                                                                                                                                                                  aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGaa                                                                                                                                         <  1:107313/183‑1 (MQ=255)
                                                                                                                                                                                                                                  aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAg                                                                <  2:332173/255‑1 (MQ=255)
                                                                                                                                                                                                                                     aGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTgcg                                         <  2:214407/275‑1 (MQ=255)
                                                                                                                                                                                                                                            acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                                                                                                                                                      <  8:565628/64‑1 (MQ=255)
                                                                                                                                                                                                                                            acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                                                                                                                                                      >  7:565628/1‑64 (MQ=255)
                                                                                                                                                                                                                                             cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGCTCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGACCTACCTgg                                 >  2:3248/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                                                                                                                                                                                                          >  8:166711/1‑103 (MQ=255)
                                                                                                                                                                                                                                                 aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                                                                                                                                                                                                          <  7:166711/103‑1 (MQ=255)
                                                                                                                                                                                                                                                 aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGa                                >  2:331372/1‑272 (MQ=255)
                                                                                                                                                                                                                                                 aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGa                                <  4:331371/272‑1 (MQ=255)
                                                                                                                                                                                                                                                           cccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                                                                                                                                                                   <  6:597764/100‑1 (MQ=39)
                                                                                                                                                                                                                                                           cccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                                                                                                                                                                   >  5:597764/1‑100 (MQ=39)
                                                                                                                                                                                                                                                           cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAttt                                                                                                                                                                                      >  6:56568/1‑113 (MQ=255)
                                                                                                                                                                                                                                                           cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAttt                                                                                                                                                                                      <  5:56568/113‑1 (MQ=255)
                                                                                                                                                                                                                                                            ccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                                                                                                                                                                             >  6:508685/1‑73 (MQ=37)
                                                                                                                                                                                                                                                            ccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                                                                                                                                                                             <  5:508685/73‑1 (MQ=37)
                                                                                                                                                                                                                                                            ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                                                                                                                                                              <  6:129535/88‑1 (MQ=255)
                                                                                                                                                                                                                                                            ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                                                                                                                                                              >  5:129535/1‑88 (MQ=255)
                                                                                                                                                                                                                                                            ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTa                                                                                                                                                                         >  6:442276/1‑125 (MQ=255)
                                                                                                                                                                                                                                                            ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTa                                                                                                                                                                         <  5:442276/125‑1 (MQ=255)
                                                                                                                                                                                                                                                             cGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCGGTAGaa                                                                                                                                                                                                                    >  5:957397/1‑81 (MQ=255)
                                                                                                                                                                                                                                                             cGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCGGTAGaa                                                                                                                                                                                                                    <  6:957397/81‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tgctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTtgatg            <  2:265198/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tactgaAACATGCCGGGGGATGGAATAGAAACAAGCTAAAGGAGAACTTCGAATGTCGGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTAGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTtgatg            <  1:117656/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGa                                                                                                                                                                     >  1:136913/1‑122 (MQ=255)
                                                                                                                                                                                                                                                                    ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGaa          >  2:168455/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                      gcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAg                                                                                                                                                                                                                >  7:122356/1‑76 (MQ=255)
                                                                                                                                                                                                                                                                      gcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAg                                                                                                                                                                                                                <  8:122356/76‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTa                                                                                                                                                                 <  3:136913/122‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aCATGCCGGGGGCGGTATGAGAAACAAGCTATAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAGAACGAAgaga                                                                                                                                     >  8:975876/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                            tGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTgg                                                  >  2:449810/1‑227 (MQ=255)
                                                                                                                                                                                                                                                                            tGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGAAGTATTGcc  >  2:196145/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                  
GAATGAATAATTAAGCGGCTTTTTTGCAAAGCGCAATTTTGCATTTTGTATAAGTATTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACCTGCGCTACCTGGACACATTAAACCGACTTGATGAAGTATTGCC  >  NC_000913/2560061‑2560603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: