Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,592,989 | A→G | 5.8% | intergenic (+27/+83) | ypfN → / ← ypfH | putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,592,989 | 0 | A | G | 5.8% | 339.3 / 8.4 | 104 | intergenic (+27/+83) | ypfN/ypfH | putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate |
Reads supporting (aligned to +/- strand): ref base A (49/48); new base G (3/3); total (53/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-03 |
Only 100 of 104 total aligned reads displayed.
CAGCTCGTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTG > NC_000913/2592720‑2593260 | cAGCTCGTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGAGGACGACTGGACGAAAAAGGATCAAACGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCg > 2:403963/1‑275 (MQ=255) aGCTCGTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGt > 1:143910/1‑275 (MQ=255) cccgtcgCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCCAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACTTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCCCGCCAATAAGCCGGACTTTTCCTCGTATCGTGATTTCAACGATAAGTGGGACGATGACGACGACCGGCCGGAAAAGGACCAACCGCAAACAGAAGCTGATGCCTAAACCGTAGGCCGGATAAACCGtt > 4:183135/3‑274 (MQ=255) cTCGTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGCTTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCGCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACCACTGGCCGCAAAAGGACCCACCGAAGAAGTAAGCTGATTTCGCCACCGTAGACACGATACGGCGtt > 4:377402/1‑274 (MQ=255) cTCGTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCACGCATCGTGATTTCAACGATACGTGGGACGATGACGACGACTGGCCGAAAACGGATCAACCGAAGCAGTAAGCTGAGTCCTCCACCGTCGGCCGTATAAGGCggt > 4:157444/1‑272 (MQ=255) aaTAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt > 2:306310/1‑274 (MQ=255) tGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTTATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAc > 2:515710/1‑274 (MQ=255) gctggctAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGACAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgc > 4:348348/1‑275 (MQ=255) ctggctAAAGATTGGTGGATTCTGATGATTGGCTGTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTAAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGAGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTGAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgcg < 2:64445/275‑1 (MQ=255) ggtggATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGt > 4:243144/1‑263 (MQ=255) gtggATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAg > 2:12843/1‑275 (MQ=255) gaggATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCTACCATAAGAAATCTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTCATTTCAACGATAAGTAGGACGATGACGACGCCTGGCCGCAAAAGGATCAACCGAAGAAGTAAGCTGAGTCCTCAACCGAAGGCCGGATCAGGCGTTAACGCCGCCTCCGACAATGGTTACCGATCCCCGAGTGGACCCTCCCGCGCCTTaacggccaa > 4:461605/3‑266 (MQ=255) gATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTa < 2:243145/263‑1 (MQ=255) ttttGGTGTTTTTGTTTTTGGTAGGCGTCNNTCTGAACGTGATTAAAACTCTCACACGCGTNAACCATAAGCAGTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGANGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt < 3:62244/272‑1 (MQ=255) ttCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATGTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGAGTTCAACGATAAGTGGGACGATGACGACGACTAGCGGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTTCTCAACCGAAGGCCGGATAAAGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTAATGCGAGCCCCACGCCTCGTACCAGGCCGGGGAAt > 4:420627/1‑275 (MQ=255) ttCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGGGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt < 1:114620/275‑1 (MQ=255) tttggtttttGTCTTTTTGGGTGGCGGCCTGCGTAACCTTATGAAAGATCCCAAGCCGGTCCACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGAGGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc < 4:515709/267‑1 (MQ=255) aTTGTCTTTTTGGTATGCGTCCGGCTGAACTGGATTACAGAGCTCAAGCGCGTCGACCATAAGAAATTACTCGCCAACAAGCCGGAGCTTCACCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGGCTGACCGAAAAAGGATCAACCGAAGAAGGAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt < 2:183136/275‑1 (MQ=255) gtttttGGTAGGCGTCCTGCTGAACGGGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAca < 2:490561/273‑1 (MQ=255) ttGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCAtt < 2:308617/275‑1 (MQ=255) aGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGGAATCTGTCTTTTACATCATTTCaa > 2:385499/1‑274 (MQ=255) ctcAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATAAACCGAAGAAGTAAGCTGAGTGCTCAAACGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAACCTGTCTTTTACCTAATTTCAATCACATCATCATCCCCAGGCTTACCGCCa > 4:150797/1‑275 (MQ=255) tcAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCTGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGGCGACCGGCCGACAAAGGATCAAGCGAAGAAGTATGCTGAGTGCTCAACCGTAGGCCGGTTAAGGCGTTAACGCCGCATCCGGCATTGGTGAAGCATTCATGATGCGAGGCCCACGCGTCTTTCCCGGCCAGGTAATATGACTTTTACCTCATTCCAACCTCATCATCATCGCAAGCCTTCCCCCCa > 4:121553/1‑274 (MQ=255) aGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg < 5:654751/148‑1 (MQ=255) cgcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg < 5:1027049/147‑1 (MQ=255) cgcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg > 6:1027049/1‑147 (MQ=255) tccATAAGAAATTTCTCGCCAAAAAGCCAGAGCGTCCCCCGGATCGTAATTTTAAAGGTAAGTAGGACGAAGACTACGACTGGCCGAAAAAGGATCAAACGACAAAGTAGGATAGGTGATCGACCGTAGGCCGGATAAGGAGTTAACGCCGCATCCGGAAATGGTGAACAATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCGGTCTATTACGTCTTTGCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgt < 4:328636/274‑1 (MQ=255) gacagaaaaaaTTTTTCGCCAACAAGCCGGGGCTTCGCCCGTTTCGTGATTTAAACGGTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCGACCGAAGAAGTAAGCTGAGAGCTCAACCATAGGCGGGATAAGGCGGTAACGCGGCATCAGGTAATGGCGAACGGTGACAGATGGGACGCGCAGGCGAGTTATCAGGCCAGGTAAACTGTCTTGTACATCAGTTCTGTCTAGTCTTCATCGACAAGATTACCGGCAGTTAGTGCTtcgg < 4:385498/267‑2 (MQ=255) aacATAAGAAATTTTTCGCCAACAAGCCCGAGCTTCCCCCGCATCGTGTATTCAACGATAAGGGAGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAATCTGAGTTCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGTCTCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgt < 2:3446/273‑1 (MQ=255) aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGt > 6:909416/1‑137 (MQ=255) aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGGGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGt < 5:909416/137‑1 (MQ=255) gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc > 6:94012/1‑148 (MQ=255) cGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg < 5:94012/148‑1 (MQ=255) ccAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCAACGCTCAGGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGaa < 4:306309/275‑1 (MQ=255) acaacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGaa < 2:348349/274‑1 (MQ=255) caaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctt > 5:843752/1‑128 (MQ=255) caaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctt < 6:843752/130‑3 (MQ=255) ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGc > 8:466228/1‑148 (MQ=255) ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGc < 7:466228/148‑1 (MQ=255) cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCTAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAAc < 1:46129/275‑1 (MQ=255) aGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg < 7:125938/110‑1 (MQ=255) aGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg > 8:125938/1‑110 (MQ=255) cTTCCCCCGCAACGGGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAg < 2:453561/275‑1 (MQ=255) ttCCCCCGCATCTGGAGTTCAACGAAAAGTGGGACGAGGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAGGCTGAGTGCTCAACCGTAGTCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGATCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCTCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAg < 4:435629/274‑1 (MQ=255) cccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGGCATCTGTCTTTTCCATCATTTCAATCACATCATCATCTACAGGCTTACCGCCACTTAGCGCTTCGTCGAACTAATGTTTCGGACTGGTATAACGCAATTg > 1:146623/1‑274 (MQ=255) ccccGCGTCGTGACTTCAACGAGAAGGGGGACGATGACGACGAAGGGCCTAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGAt < 2:157445/275‑1 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCg < 8:144015/105‑1 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCg > 7:144015/1‑105 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg > 8:501030/1‑111 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg < 7:501030/111‑1 (MQ=255) ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgccttatc > 8:619800/1‑112 (MQ=255) ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgccttatc < 7:619800/117‑6 (MQ=255) cGTGATTTAAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGc < 2:469928/274‑1 (MQ=255) tGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCCCTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTCTAACGCAAGTGATCCAGGGCaaa > 1:14909/1‑275 (MQ=255) tGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGTTCCAGGGCaaa > 2:480221/1‑275 (MQ=255) cAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc > 6:331378/1‑148 (MQ=255) ctAAGTGGCACGATGACGACGACTGGCCGAACCCGTCTCAACCGAAGAAGTAAGCCGAGTGCTCAATCGTAGGCAGGATAAGGCGTTCGCGCGGCAACCGGCAATGGTGAAAGAAACCCAATGAAACGCTCCCGCGTCCTATCCGGcc < 8:520941/147‑1 (MQ=255) aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATg > 6:975942/1‑116 (MQ=255) aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATg < 5:975942/116‑1 (MQ=255) gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg < 5:505065/76‑1 (MQ=255) gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg > 6:505065/1‑76 (MQ=255) gACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg < 7:1066427/126‑1 (MQ=255) gACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg > 8:1066427/1‑126 (MQ=255) gACGAGGACAACGACTGGCCGAAAAAGGAACAACCGAAGAAGTAAGTGGAGTGCTCAACCGTAAGCCGGATAAGGCGTTAACGCGGCATCCAGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCg < 2:121554/275‑1 (MQ=255) cGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTCAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg < 5:331378/148‑1 (MQ=255) tGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACg > 2:268038/1‑115 (MQ=255) gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCa < 4:268037/115‑1 (MQ=255) cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGTGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCTAGGGCAAATTGCATACTGCGATTGTCGATCGAATGGcc > 1:4882/1‑275 (MQ=255) ggCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGa < 8:360037/86‑1 (MQ=255) ggCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGa > 7:360037/1‑86 (MQ=255) gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATTCAGGGCAAATTGCATACTCCGATTGTCGATCGCATGGCCTAAAtc > 2:484180/1‑275 (MQ=255) cGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg > 8:468507/1‑80 (MQ=255) cGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg < 7:468507/80‑1 (MQ=255) cGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAAtcct < 1:11146/275‑1 (MQ=255) aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctta > 7:898689/1‑64 (MQ=37) aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc < 8:1005702/69‑1 (MQ=255) aaaaGGATCAACCGAAGATGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa > 8:717657/1‑74 (MQ=255) aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa < 7:717657/74‑1 (MQ=255) aagattCACCCAAAGAAATAAGNNCAGGTNTCACCCATTGTCCGGATAATGCTTTACCCCCGCATCCGGCAATTGTGAACTATACCTGTTCCGACGCTCCCGCATCTTATCAGGCCGAGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCTCCAGACTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGGCTAAATCCTCCACg < 3:4882/270‑1 (MQ=255) tCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATc < 2:464655/275‑1 (MQ=255) agaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgc > 8:70713/1‑47 (MQ=255) agaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgc < 7:70713/47‑1 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt > 8:1093707/1‑93 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt < 7:1093707/93‑1 (MQ=255) aGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTaaa > 2:138508/1‑246 (MQ=255) gAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAAtcct < 4:138507/246‑1 (MQ=255) tGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTc > 2:540076/1‑173 (MQ=255) gtcccAACCGGAGGCCGGGTAAGGCGGTAACGCCGAATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCa < 4:46979/271‑1 (MQ=255) cAACCGTAGGCCGGATAAGGNGTTANCGCCGCATCCGGCAATGGTGAACNATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGaa < 4:540075/173‑1 (MQ=255) aCCGTATGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg > 6:612003/1‑81 (MQ=255) aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg < 5:612003/81‑1 (MQ=255) aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcat > 5:1039169/1‑120 (MQ=255) aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcat < 6:1039169/120‑1 (MQ=255) tAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCtt > 2:558281/1‑129 (MQ=255) ggCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATCACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATCTCCAGCGTCACGTCACCACCGGCCCTGATTAAccc > 4:90972/1‑272 (MQ=255) ccGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCg < 4:558280/129‑1 (MQ=255) gATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCACTCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTACACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCt > 2:223801/1‑274 (MQ=255) gATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTg > 2:184245/1‑275 (MQ=255) gATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTg > 2:123203/1‑275 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTCATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCCTGGCCTAAACCCTCCACGACATCCAGCACCACCACCCCACCTGCACTACTTAACCCCTcccg > 4:270694/1‑272 (MQ=255) | CAGCTCGTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGTATAACGCAAGTGATCCAGGGCAAATTGCATACTGCGATTGTCGATCGCATGGCCTAAATCCTCCACGATATCCAGCGTCACGTCACCACCGGCACTGATTAACGCTTCCTG > NC_000913/2592720‑2593260 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |