| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 53,376 | G→A | 7.4% | P14L (CCC→CTC) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 53,376 | 0 | G | A | 7.4% | 364.2 / 15.0 | 117 | P14L (CCC→CTC) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (54/54); new base A (5/4); total (59/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CGCTCGCGCCAGCGTTTCCACCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCAC > NC_000913/53105‑53642 | cGCTCGCGCCAGCGTTTCCCCCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCt < 2:138008/275‑1 (MQ=255) ccAGCGTTTCCACCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGa < 2:276239/275‑1 (MQ=255) aGCGTTTCCACCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt < 4:284195/275‑1 (MQ=255) aGCGTTTCCACCACAAAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACGGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt < 2:223200/275‑1 (MQ=255) cTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAGCCGAGCATCGCTGCCCGGTTGGNAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACCCAAGCTGAGCAAGCGGGAAAACTAAACCCGNGCCAATGCCGGCGGGCCCGCCGGGGGGGA‑CNNAACAACGGTGGGTGGTAACCaagtgtggc > 3:62563/1‑267 (MQ=255) cTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCCATCCCAGCGGGCCCCGCGGGGGGGATCACAAC‑ACGTTTGGTTTTAACCATTAGGTg > 4:440464/1‑274 (MQ=255) ggTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGgcg < 1:106703/275‑1 (MQ=255) cACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTgg < 2:27303/275‑1 (MQ=255) cACGTAGCGCGACAGGAAGTACGGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGCTGGGGGAATAAGGGCCGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGTGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGTGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTATTTGCTCAGGATTTTAACGTAGGCGCTgg < 1:62563/275‑1 (MQ=255) aGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCACCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGATAACTAAGTCCGGGCCAATCCCGGCGGGCCCGCCGGGAGTGAACACAAC‑CCGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTgac > 2:18471/1‑271 (MQ=255) gctgttgcggcgggTTGGGGGAATCAGGGTGCTGGTTGAACGGCAAACCCGGGATCGTGTCCCGGTTGGGACGGGGAGTGGCATCGGCCCAAACAACCAGTCCGACCGGCCCCCAACGCTGTGCAAGCTGGACAACTAAGTCCTGGCCAATCCCGGCCGGCTCGCCGGGAGTGTTCACCAC‑ACGTTGGGTTTTACCCATTCGTTGCTCAGGATGTTAACGTAGGCGCTTGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCAAGAACTTa < 4:380096/261‑1 (MQ=255) tgCAGGGGCGTTGGGGGAATAAGCGCGGCGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTAGGCACGGAGCGTGGCACCGGCCCACACAACCAGTTCGCCCGGCCACTCCCGCTGTGCAAGCTGGCCCACTAAGTCNGGGCCAATCCCGGCCAGCCCGCCGGGAGTCATCCCCAC‑ACGTTGCCTTTTCACCATTACTTCCCAACGATTTTGNCGTCGACCCTGGCTCTTTGCTTTTCCAGCCNNCTTTCTTCTTCCTGCgagcattttgcg > 3:70702/1‑264 (MQ=255) ttGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCa > 2:307671/1‑273 (MQ=255) gggggAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCAAAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCg < 2:377328/275‑1 (MQ=255) ggggAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGc > 7:521784/1‑148 (MQ=255) gAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg > 5:589112/1‑147 (MQ=255) aaTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCggg > 6:705782/1‑148 (MQ=255) aTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGa < 8:1044339/148‑1 (MQ=255) cGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTg < 5:504521/144‑1 (MQ=255) cGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTg > 6:504521/1‑144 (MQ=255) gAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTACCGTAGGCGCTTGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTCCGGTTCATCCGCATGCTGTATCCCCGCTCttt > 4:368639/1‑275 (MQ=255) gAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGt > 5:280837/1‑148 (MQ=255) gAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTAGGCACGATCTTTCTgcgc > 2:113691/1‑275 (MQ=255) aCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTa > 5:991695/1‑148 (MQ=255) aGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCa > 7:28191/1‑148 (MQ=255) tCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCa < 6:376648/144‑1 (MQ=255) tCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCa > 5:376648/1‑144 (MQ=255) gCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGtt > 5:1010231/1‑148 (MQ=255) tGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTg > 8:622158/1‑147 (MQ=255) tGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTg < 7:622158/147‑1 (MQ=255) cccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATc > 7:269118/1‑116 (MQ=255) cccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATc < 8:269118/116‑1 (MQ=255) cccGGTTGCTACGACGGGTGGCCACGGACCAAACAACCAGTTCGACCGCCCACTCACGCTAGACAAGCTGGACAACTTAGTCCGTGCCAAGCCCGGCGGGCTCGCCGGTGGTGATAACAAC‑ACGTTGAGTTTTAACCGTTAAGTGCTCAGGATTTTAACGTAGGCGCTGGCTCGTTGTGCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACGTACGGTTCATCAGCATGCGGTATGCACGATCTTTCGGCGGAGCGTCGGTTTTATCGACATTAc < 4:18471/275‑1 (MQ=255) ggTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAgg < 8:521784/148‑1 (MQ=255) ggTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGAttt < 8:28191/148‑1 (MQ=255) aaGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCACTCCCGGCGAGCCCGCCGGGatt > 8:571379/1‑100 (MQ=255) aaGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGa > 5:236154/1‑142 (MQ=255) aaGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGa < 6:236154/142‑1 (MQ=255) aaGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTaa < 5:705782/148‑1 (MQ=255) agagTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTa > 5:18470/1‑148 (MQ=255) tGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcatca > 3:97935/1‑206 (MQ=255) ggCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATca < 7:210770/98‑1 (MQ=255) ggCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATca > 8:210770/1‑98 (MQ=255) cATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCacaa > 7:1069269/1‑99 (MQ=255) cATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCa > 4:433898/1‑117 (MQ=255) aTCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCAt < 1:97935/206‑1 (MQ=255) cGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTggg < 7:922012/105‑1 (MQ=255) ggCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAg < 2:433899/117‑1 (MQ=255) gCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTa < 6:456810/131‑1 (MQ=255) gCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCCCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTGGCTGCTTCTTCCGAGCACTTACGGTTCATCAGCATGCGGTTGGCACGCTCTTTCTGCACAGCGTCGGTTTTATCCACATTACGGGTCTCCAGCATCTCGGTTAAAAGc > 4:485334/1‑275 (MQ=255) cacaAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTa < 5:940347/107‑1 (MQ=255) cacaAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTa > 6:940347/1‑107 (MQ=255) cacaAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCggg > 7:899886/1‑82 (MQ=255) cacaAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCggg < 8:899886/82‑1 (MQ=255) aacaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTg > 5:736204/1‑82 (MQ=255) aacaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTg < 6:736204/82‑1 (MQ=255) aacaacCAGTGCGACCGTCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTGTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTGCGATTAAATGCCAGc < 1:70702/274‑1 (MQ=255) acaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGcc < 5:410660/74‑1 (MQ=255) acaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGcc > 6:410660/1‑74 (MQ=255) caacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTcc < 6:18470/148‑1 (MQ=255) caacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTcc < 6:991695/148‑1 (MQ=255) aacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTaa > 5:770254/1‑101 (MQ=255) aacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTaa < 6:770254/101‑1 (MQ=255) cAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCAtt < 6:104204/103‑1 (MQ=255) cAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCAtt > 5:104204/1‑103 (MQ=255) aGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTgg < 8:160927/132‑1 (MQ=255) aGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTgg > 7:160927/1‑132 (MQ=255) cTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGctt > 5:1071788/1‑148 (MQ=255) aCGCTGTGCAAGCGGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCa < 1:44211/273‑1 (MQ=255) tgtgCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTAc > 2:531018/1‑216 (MQ=255) gtgCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACgtt < 6:192116/116‑1 (MQ=255) cAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGt < 4:531017/216‑1 (MQ=255) gACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGaa > 8:65555/1‑140 (MQ=255) gACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGaa < 7:65555/140‑1 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGAtt < 5:920219/83‑1 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGAtt > 6:920219/1‑83 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTaa < 5:153421/87‑1 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTaa > 6:153421/1‑87 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTc > 1:67664/1‑275 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGGATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCg > 2:209437/1‑236 (MQ=255) cAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTAAGAAGAAC‑ACGTTGGGTTTGAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGAATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGGGTGAACCGGTGCACTCATTTGACCTTTGTTc < 4:307670/274‑1 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcatc < 6:1071788/148‑1 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcatc < 8:116708/148‑1 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCg < 2:556418/156‑1 (MQ=255) aaGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTg > 7:366695/1‑68 (MQ=255) ggCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAggg > 2:479392/1‑275 (MQ=255) gCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGc > 2:363428/1‑275 (MQ=255) cAATCCCAGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 2:440465/275‑1 (MQ=255) aaTCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCg < 6:798148/118‑1 (MQ=255) tCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAag > 2:352870/1‑224 (MQ=255) tCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTTCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGctcccc > 4:223415/1‑270 (MQ=255) tCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTACATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTNCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGCCATTACGGGTATCCAGCGGNTCGATTAAATCCCAGCCGACAGAAGAGTGAACCGGTGCACTCATTNGNACTTTGTTCAGGCGCGTCAGGGCGGCAc > 3:40491/1‑275 (MQ=255) tCCCGGCGAGCCCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCAtt > 6:389783/1‑50 (MQ=37) tCCCGGCGAGCCCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCAtt < 5:389783/50‑1 (MQ=37) cccGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTcc < 5:842748/90‑1 (MQ=255) cccGGCGAGCCCGCCGGGATTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCAc > 5:146580/1‑148 (MQ=255) ccGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGgcgc > 5:937099/1‑75 (MQ=255) ccGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGgcgc < 6:937099/75‑1 (MQ=255) ggcggGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTg < 4:352869/224‑1 (MQ=255) gcggGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATc > 6:774906/1‑148 (MQ=255) | CGCTCGCGCCAGCGTTTCCACCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAAC‑ACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCAC > NC_000913/53105‑53642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |