| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,653,104 | G→C | 8.8% | W204S (TGG→TCG) ‡ | sseA → | 3‑mercaptopyruvate sulfurtransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,653,104 | 0 | G | C | 8.8% | 438.5 / 27.3 | 130 | W204S (TGG→TCG) ‡ | sseA | 3‑mercaptopyruvate sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base G (60/58); new base C (7/5); total (67/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-03 | |||||||||||
Only 100 of 130 total aligned reads displayed.
GAGAAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACA > NC_000913/2652833‑2653349 | gagaAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGa < 2:443988/274‑1 (MQ=255)gagaAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCCCGGGCATATTCCCGGGACACTTAATGTTCCGTGGAc > 4:400023/1‑275 (MQ=255) aaGGGTGGATTCTGTGCGGGGGAGTTGCAGGCTGGGAGGGCGAGGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAGCGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGaa < 4:145919/275‑1 (MQ=255) gCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGAACGTTg > 2:170756/1‑275 (MQ=255) agGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCTATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGTTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTTCCCCAAGGCGGACTAAAAACGACCGATGAACTGGCTGCGATATTTTTTGGTcgc > 2:9956/1‑274 (MQ=255) gTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTTAATGTTCCGTCGACGGAACTGGTCCGCACAGGCCAACTAAAACCGACCGTGTACCGGGATCGGATTTTTGTTGGTCccggc > 4:25791/1‑275 (MQ=255) gTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCgcggc > 2:315087/1‑275 (MQ=255) gTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTGTAACGCAGAAGGTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGGGCGACGGCGAACTAAAAACGACCGATGAACCGGATGCGAAATTTTTTGGTTgcggc > 1:141597/1‑275 (MQ=255) ggatgtcTGCCGGAAGGAGAGTTTAAAGCGGTGTTTAAGCCTGAAGCCGTGGGTAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAg < 4:352010/269‑1 (MQ=255) cTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa > 2:522959/1‑275 (MQ=255) ttccGGAAGGAAAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATTTATTATTGGCAAGCCATAAAAATACGGCGCAAATTATTGGTGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACg < 1:140433/271‑1 (MQ=255) ccGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGGGTCAGCTACGACaa > 1:111531/1‑275 (MQ=255) tAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCGGCTACGACAAACGAATTATCGTCa > 2:216024/1‑275 (MQ=255) ccgcGGTTATGCCTGGATCCGTGGTGAAAGTAACCGATGTATTCTGGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGGCGGAACTGGTGCGCGAAGGCGAACTAAAAGCGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTg < 4:216023/274‑1 (MQ=255) cgTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCAATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGAAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCgg < 1:104843/274‑1 (MQ=255) gTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGc < 2:155995/274‑1 (MQ=255) tttAAGCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGct < 2:25792/274‑1 (MQ=255) tgatgccTGAAGCCGTGGTGAAATTAACAGATGTATTATTGGGAAGCTATGAAAATACGGCGTAAATTATTGAGGGCCGCCCGGTTGAACGTTTTAACGCAGAAGTGTATGAACCTCGCCCAGGGTTACGTCGCGGACATATTACCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGctc < 4:9956/269‑1 (MQ=255) tGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTg > 2:125318/1‑269 (MQ=255) tGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTaa > 2:418645/1‑275 (MQ=255) gtggtgAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAACGCGAACTAACAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGTCTCTGGTGTAACGGCAg > 2:65502/1‑274 (MQ=255) caaGTAACCGTGGGAGTATTGGCAAGCCATTAAAATACGGGGCAAATTATTGATGCCGCCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAAGGTTCCGGGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGt < 4:55745/274‑1 (MQ=255) aaaGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGt < 2:400024/275‑1 (MQ=255) aTGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGt > 6:353144/1‑148 (MQ=255) tGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTg < 5:324810/148‑1 (MQ=255) attGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGaa > 6:196256/1‑148 (MQ=255) ttGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAc < 7:11279/136‑1 (MQ=255) ttGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAc > 8:11279/1‑136 (MQ=255) ggCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGc < 5:353144/148‑1 (MQ=255) gCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTACAAACGACCGATGCACTGGGTGCGATATTTTTTGGTCGTTGCGTAGGCTACGACAAACCAATTATCGt > 2:522953/1‑221 (MQ=255) agaaaTGAAAATCATGCGCAAATTATGGNGNCCCCCCCGGCTGCACGTTTTAACGCAGAAGTTGATGACCCTCGCCNAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGANCTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGa < 3:44021/271‑1 (MQ=255) gCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACt < 7:997892/148‑1 (MQ=255) gCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGc > 1:44021/1‑270 (MQ=255) tGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaa > 8:195322/1‑148 (MQ=255) gAAAATACGGGGCAAAGTATTGATACCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGcc < 2:505733/275‑1 (MQ=255) gAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGcc < 4:418644/275‑1 (MQ=255) gAAAATAAGGGGCAAATTATTGAGTCCCGCCCGGCTGCACGTTTTAACGAAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGAGGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGcc < 4:522958/275‑1 (MQ=255) aaaTACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACg < 5:196256/148‑1 (MQ=255) aaaTACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACg < 6:1014458/148‑1 (MQ=255) ggCGCAAATTATAGCTGCCGGACCGGCTGACAGTTTTAACGCAGAAGGTTATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGACCTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGa < 2:519007/275‑1 (MQ=255) cgcAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGGGCCAAACGGGaaa > 2:256891/1‑275 (MQ=255) cccgcccgGCTGGATGTTTTAAAGTGGAAGTTGATGAACCGCGCCCAGGTTTTCGTCGCGGACATATTCCCGGAGAACTGAAGGTTCCGTGGACGGAACTGGTGCGCGAGGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCa < 4:170755/275‑1 (MQ=255) cACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAgg > 8:862907/1‑100 (MQ=255) ttttAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAATAACGACCGATGaa < 7:82155/118‑1 (MQ=255) tttAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt > 7:1032/1‑134 (MQ=255) tttAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt < 8:1032/134‑1 (MQ=255) ttAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGgcgcg > 2:183953/1‑275 (MQ=255) cAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGa > 8:750856/1‑73 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGa > 8:399299/1‑89 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGa < 7:399299/89‑1 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACg > 5:652030/1‑99 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACg < 6:652030/99‑1 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACCCGCGACGCTGGA‑TGTGCCAAACGGGAAACTGTACGGCGGCCCATGGAGTGAATGGGGCGCGCGGGCAg > 4:219428/1‑274 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAc > 6:758707/1‑73 (MQ=255) gAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAc < 5:758707/73‑1 (MQ=255) gAAGTGGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaa > 8:542382/1‑96 (MQ=255) aaGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCGCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TTTGCCAAACGTGAAACTGTACTACGGCGCATGGAGTGAATGGGGCGCGCGGGCCGa > 2:270854/1‑274 (MQ=255) aaGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGAACGATGAAATGGCTGCGATATTTTTTGGTCGCGGCGGCAGCTACGACAAACCAATTATCGTACGCTGCGGCTCTGGTGTAACGGCCGCCGTGGTTTTGTTAGCACCCACGCAGCTGGA‑GTTTACAAACGGGAACCTGTAAGCCGGCGCATGGGGTACATGGGGCCCGCggagcaaa > 4:6359/1‑269 (MQ=255) ttGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCa < 2:126056/245‑1 (MQ=255) tgatgaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt > 8:659026/1‑120 (MQ=255) tgatgaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt < 7:659026/120‑1 (MQ=255) aCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGa < 7:323497/102‑1 (MQ=255) aCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATg < 5:84110/104‑1 (MQ=255) aCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATg > 6:84110/1‑104 (MQ=255) cTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGt > 7:753700/1‑148 (MQ=255) tcccATGTTTACGTCGGGGACAAATTCCCGGAGTACGGAATGTTCCGTGGACGGAACTGGTGCGTGAAGGCGAACCAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGGGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGcc < 4:150399/273‑1 (MQ=255) cccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAAc > 2:231601/1‑211 (MQ=255) ggTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg < 8:280405/106‑1 (MQ=255) ggTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg > 7:280405/1‑106 (MQ=255) ggTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCg < 8:282229/148‑1 (MQ=255) cGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACg < 8:897783/118‑1 (MQ=255) cGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACg > 7:897783/1‑118 (MQ=255) cGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGACTGT‑CCAAACGTGAAACTGTACGGAGGCACACGGGGGTACTGGGGCGCGCGGGCAGATTTAACGGTTGAGCCCGGGAAATAAgg > 4:422057/1‑274 (MQ=255) cgGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTa < 8:753700/148‑1 (MQ=255) cgGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACCCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGGCGATTTACCGGTTGAGACAGTGAAATAAGGAtttt > 4:187606/1‑275 (MQ=255) ggACATATTCCCGGGGAACTGAAGTTTCCGGGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACAGAGGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTa < 4:183952/275‑1 (MQ=255) gACATATTCCCGGAGCACTGAATGTTCCGTGGACGGa > 5:665901/1‑37 (MQ=255) gACATATTCCCGGAGCACTGAATGTTCCGTGGACGGa < 6:665901/37‑1 (MQ=255) gACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAAc > 5:769194/1‑148 (MQ=255) ttCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTgg > 7:66059/1‑72 (MQ=255) ttCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt < 7:822385/86‑1 (MQ=255) ttCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt < 7:689105/86‑1 (MQ=255) ttCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt > 8:689105/1‑86 (MQ=255) ttCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt > 8:822385/1‑86 (MQ=255) cccGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGctct > 7:909482/1‑132 (MQ=255) ccGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGt < 6:769194/148‑1 (MQ=255) gAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACaaa < 7:239114/97‑1 (MQ=255) gAATGTTCCGTGGACGGAACTGGTGCGCGAAGACGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACaaa > 8:239114/1‑97 (MQ=255) aaTGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTacgacg > 2:280811/1‑190 (MQ=255) cagTTCCGTCCACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt > 7:1118734/3‑71 (MQ=255) aTGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCgcgg < 5:697350/79‑1 (MQ=255) aTGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCgcgg > 6:697350/1‑79 (MQ=255) aTGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCaa > 8:304871/1‑99 (MQ=255) aTGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCaa < 7:304871/99‑1 (MQ=255) gTTCCGTCCACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCg > 5:743231/1‑133 (MQ=255) gTTCCGTCCACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCg < 6:743231/133‑1 (MQ=255) ttCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGgcgc < 4:280810/190‑1 (MQ=255) tCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTc > 7:266981/1‑148 (MQ=255) tCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTc > 7:396909/1‑148 (MQ=255) tGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGa < 8:396909/148‑1 (MQ=255) | GAGAAAGTGTCGATTCTGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAATCCTGAAGCCGTGGTGAAAGTAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGA‑TGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACA > NC_000913/2652833‑2653349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |