Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,367 G→A 15.6% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,3670GA15.6% 106.7 / 14.4 45intergenic (+28/‑488)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (13/25);  new base A (4/3);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-05

ACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATA  >  NC_000913/2653106‑2653597
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      
aCGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCgg                                                                                                                                                                                                                              >  4:278549/1‑272 (MQ=255)
     aCTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAt                                                                                                                                                                                                                      <  2:278550/275‑1 (MQ=255)
       tggtggCCGAAGGGGAACTAAAAACGACCGATGTACTGGATGCGAGATTTTTTGGTGGCTGCGTCAGCTACGACAAACCAAGTATCGTCAGCGGCGGGTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAGCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTg                                                                                                                                                                                                                    <  4:65501/275‑1 (MQ=255)
             gcgAAGGGGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTGGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGGGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGGCGAATTTGGCGGTTTTTTATTGCTAGt                                                                                                                                                                                                               <  2:128825/274‑1 (MQ=255)
                       aCTAAAATCAACCGATGAACTGGATGCGATATTGTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcgcg                                                                                                                                                                                                     <  2:172063/274‑1 (MQ=255)
                       aCTAAAAACGACCGAGGAACTGGATGCGAGATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTGTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcgcg                                                                                                                                                                                                     <  2:219429/274‑1 (MQ=255)
                                 aCCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCAGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagc                                                                                                                                                                                          >  2:104647/1‑275 (MQ=255)
                                                                          cTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg                                                                                                                                                 <  2:422058/275‑1 (MQ=255)
                                                                           tACGAAAACCCAATTATCGTCAGCTGCGGCTCTGGGGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCAAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg                                                                                                                                                 <  2:491213/274‑1 (MQ=255)
                                                                                   aCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACaa                                                                                                                                         <  2:187607/274‑1 (MQ=255)
                                                                                             gTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGATCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGt                                                                                                                              >  2:481900/1‑275 (MQ=255)
                                                                                               cAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTaa                                                                                                                            <  4:260684/275‑1 (MQ=255)
                                                                                                                     gCAGCCCTGGTTTTTTTAGCACTCGGAACGCTGGATGGGCCAAACGTGAAACTGTATGTCGGCGCATGGAGTGAATGGGGCGCGCGTGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCaaaa                                                                                                       <  4:104646/274‑1 (MQ=255)
                                                                                                                               ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatt                                                                                             <  2:6359/274‑1 (MQ=255)
                                                                                                                               ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGtt                                                                                                                                                                                                                            >  8:832107/1‑147 (MQ=255)
                                                                                                                               ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGtt                                                                                                                                                                                                                            <  7:832107/147‑1 (MQ=255)
                                                                                                                                                            ccAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGaa                                                                                                                                                                                                                                      >  6:806157/1‑108 (MQ=255)
                                                                                                                                                            ccAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGaa                                                                                                                                                                                                                                      <  5:806157/108‑1 (MQ=255)
                                                                                                                                                               aaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                                                                                                                                                  >  7:599724/1‑109 (MQ=255)
                                                                                                                                                               aaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGAAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                                                                                                                                                  <  8:599724/109‑1 (MQ=255)
                                                                                                                                                                           tACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCNCGGCCTTTCCAGCAGGTTGACTTNTGTTNCATGAGCa                                                                                                                                                                  >  4:539024/1‑161 (MQ=255)
                                                                                                                                                                           tACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCaa                                                                                                             >  4:303124/1‑214 (MQ=255)
                                                                                                                                                                             cgacgGCGCAGGGGGGGAATGGGGCGGGCGGGCAGATTTACCGGTTGGGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTc                                              <  4:481899/275‑1 (MQ=255)
                                                                                                                                                                               acgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGc                                                                                                                                                              <  2:539025/161‑1 (MQ=255)
                                                                                                                                                                               acgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCaa                                                                                                         <  2:303125/214‑1 (MQ=255)
                                                                                                                                                                                  tggattGGAGGGAATGGGTGGCGTGGGTAGATTTACCGTTAGGGATAGTGCAATAAGTAGTTTAATGGCAATAAAAAACCGCCGAAGTTGGCGGTTTTTTATTGCTAGTCTGGTTCTGTGCCTTTCCAGCAGGTTGACCTGTGTTACATGAGCAACGCAGGTGATTCAGAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTCGGta                                          <  4:105904/269‑1 (MQ=37)
                                                                                                                                                                                         gAGTGAATGGGGCGCGCGGGCAGATGTACCGGGAGAGCCAGTGAAAGAAGTTTGTTACAGGCAATAAAAACCAGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCATTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCtt                                                                                                                                                      <  1:51899/159‑1 (MQ=255)
                                                                                                                                                                                             gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                                                                                                                                                            <  6:775596/85‑1 (MQ=255)
                                                                                                                                                                                             gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                                                                                                                                                            >  5:775596/1‑85 (MQ=255)
                                                                                                                                                                                                    gcgcggGTGCAGATTTACGTGTTGAGGCAGTGAAATAAGGATTTTACAGGCAATAAAAGACGTGCGAATTTGGCGGTTTTTTATTCCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACGTGTGTTACATGAGCACCGAAGGTGCTTCACAGCAAAACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTCGGTAATAAAATAACCTGGCtta                        <  4:256890/274‑1 (MQ=38)
                                                                                                                                                                                                        gcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCatt                                                                                                                              >  1:125849/1‑166 (MQ=255)
                                                                                                                                                                                                            gCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAAct                                                                                                                          <  3:125849/168‑1 (MQ=255)
                                                                                                                                                                                                                 tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                                                                                                                                                                   >  8:206616/1‑58 (MQ=255)
                                                                                                                                                                                                                 tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                                                                                                                                                                   <  7:206616/58‑1 (MQ=255)
                                                                                                                                                                                                                                                     gCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACa      >  4:337554/1‑243 (MQ=255)
                                                                                                                                                                                                                                                        aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                                                                                                                            <  8:575511/90‑1 (MQ=255)
                                                                                                                                                                                                                                                        aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCCGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                                                                                                                            >  7:575511/1‑90 (MQ=255)
                                                                                                                                                                                                                                                         tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAata  <  2:337555/243‑1 (MQ=255)
                                                                                                                                                                                                                                                              aagcgccaAATTCGGCGGTTTTTTATTGCCAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                                                                                                                                                    <  7:831782/89‑1 (MQ=37)
                                                                                                                                                                                                                                                              aaCCGCCAAATTCGGCGGTTTTTTATTGCCAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                                                                                                                                                    >  8:831782/1‑92 (MQ=37)
                                                                                                                                                                                                                                                               aCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCa                                                                                                                                                             >  6:947307/1‑82 (MQ=255)
                                                                                                                                                                                                                                                               aCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCa                                                                                                                                                             <  5:947307/82‑1 (MQ=255)
                                                                                                                                                                                                                                                               aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt                                                                                                                   >  5:188210/1‑124 (MQ=255)
                                                                                                                                                                                                                                                               aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt                                                                                                                   <  6:188210/124‑1 (MQ=255)
                                                                                                                                                                                                                                                               aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCa                                                                                                              >  6:238520/1‑129 (MQ=255)
                                                                                                                                                                                                                                                               aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCa                                                                                                              <  5:238520/129‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTAc                                                                                                                                                                         >  6:202752/1‑66 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTAc                                                                                                                                                                         <  5:202752/66‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa                                                                                                                                             >  5:751229/1‑94 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa                                                                                                                                             <  6:751229/94‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt                                                                                                                   >  5:175802/1‑120 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt                                                                                                                   <  6:175802/120‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt                                                                                                 >  4:328795/1‑138 (MQ=255)
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      
ACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATTTAGCGTATTATCGACACCGGCCCTTTCCGCCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATA  >  NC_000913/2653106‑2653597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: