| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 328,375 | A→C | 8.2% | I120S (ATC→AGC) | betB ← | betaine aldehyde dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 328,375 | 0 | A | C | 8.2% | 402.2 / 24.6 | 127 | I120S (ATC→AGC) | betB | betaine aldehyde dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (60/55); new base C (7/4); total (67/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
CACGCCCGGCAACACGTTAAATACGCCGTCCGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAACGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGGCGCGATCGACATCCTCGCGCCCGGCGGCCTGCACGGTCGCCAGCACGTTACCGTTGGCCGG > NC_000913/328110‑328649 | cACGCCCGGCAACACGTTAAATACGCCGTCCGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAACGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCgg < 2:127046/275‑1 (MQ=255) accGGCAACCCGGTAAAAACGCCGTCCGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAGCGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCgta < 4:394671/274‑1 (MQ=255) ccGTCCGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAACGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGCCGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCAc > 2:153958/1‑274 (MQ=255) gTCCGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAACGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTCTACCCAAACGCCCTTTCCCGCACAGGGATCTGGCCGCCTTCCAGCCCC‑GGGATCAGCCCCGCCTCATCCTCCCGCCCCTCCGCACCgg > 4:334385/1‑275 (MQ=255) cGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAACGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCg < 2:219535/247‑1 (MQ=255) ggacggCCCGCTGCGCTGTAAATTTCCNCCGGCTTTGCGGCGGTAAGCGGGGTNACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCACCGCAGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTANTTACATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTTCAGCACGTCCGCACCGGTa < 3:86340/269‑1 (MQ=255) cagcagccTTTAACGCGGGAACGGGGGTACATGCGCTCGGTCTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGAGCGGATTTCCACAGGGACACCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GTGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGa < 4:153957/267‑1 (MQ=255) ttgtttggCTGGGTTTGAATTNNNNTTCGNGGGCGGCCGCCAGCGCCGGGGCGTGTGTCCACAGGGCACTCTGGATCGGGTAGTGCCATGCACCAAGCCCGGCCACTACGCCCAGCGGTTCGCGGCGTGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTcc < 3:589/268‑1 (MQ=255) aTTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCCCGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCGGGTGGCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTggc > 4:200399/1‑273 (MQ=255) aTTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGc > 2:183231/1‑274 (MQ=255) cGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGGCATTGCGTTCa > 2:461533/1‑275 (MQ=255) ttGCCGGCCGCCAGCGCCGGTGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACg < 2:533684/275‑1 (MQ=255) ggggCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCgt < 7:717104/148‑1 (MQ=255) ttCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCgg > 5:1016240/1‑136 (MQ=255) ttCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCgg < 6:1016240/136‑1 (MQ=255) acaGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGcc > 8:14161/1‑129 (MQ=255) acaGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGcc < 7:14161/129‑1 (MQ=255) ggCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTCCCAGTTTTGCGAGTTCGTAATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAg > 2:470220/1‑274 (MQ=255) ggCAAACTGGAGCGGGTAGTCCCATGGGCCAACCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAg < 4:183230/274‑1 (MQ=255) aTCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCgtagta > 8:769571/1‑125 (MQ=255) aTCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTCTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCTTTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATCCGCGAGCGCTCCATGGc > 1:159392/1‑275 (MQ=255) ggTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGa < 5:439519/105‑1 (MQ=255) ggTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGa > 6:439519/1‑105 (MQ=255) tAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCGGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGatat > 8:731707/1‑148 (MQ=255) ttCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTcc > 2:348311/1‑129 (MQ=255) cATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGCTGATCCCgg > 8:695611/1‑106 (MQ=255) cATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGCTGATCCCgg < 7:695611/106‑1 (MQ=255) cATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGCTGATCCCgg > 6:393730/1‑106 (MQ=255) aTGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCAc < 4:348310/129‑1 (MQ=255) aTGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGt > 8:1004309/1‑148 (MQ=255) tGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCCGAATATCAACGGCCCGACGCACAAGACGCGAGCGCTCAATGGCGGTCATCGACTcc > 2:189437/1‑274 (MQ=255) tGCGACAATCCCTGCCCCGACGCCCCGCGGGTGGCGGCGGGGTTACCCCAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGGAGTACTCCAGCACGTCCGGACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTGTCCGGTGTCGAGGGTTTCCAGTGTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGcc < 4:288745/274‑1 (MQ=255) gcgcCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGccc > 2:323361/1‑274 (MQ=255) cgcCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGa < 7:826913/148‑1 (MQ=255) ccAATCCCTGCCCCTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACg < 7:96922/117‑1 (MQ=255) aTCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGttt < 7:1004309/148‑1 (MQ=255) tGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGtt > 4:95972/1‑200 (MQ=255) ccaccGCCCGGCGGGTCGTGGCGGGTTAACACAAAGGCCTTTTCACGCAACGGGATCTGGCTGCCTTCCCGCGCC‑GGGATCAGCCCGGCGTAGGACTCCAGCACGTCCGCACCGGTAACGATATCTACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGCCCAGATTTTTTGccc < 4:239951/268‑1 (MQ=255) aCTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGGTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACg < 2:95973/200‑1 (MQ=255) cTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGc < 7:731707/148‑1 (MQ=255) cGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTc < 5:105023/136‑1 (MQ=255) cGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTc > 6:105023/1‑136 (MQ=255) aGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTaa > 5:762797/1‑109 (MQ=255) aGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTaa < 6:762797/109‑1 (MQ=255) gCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTGGTACTCCAGCACGTCCGCACCGGTAACGata < 8:188907/113‑1 (MQ=255) gCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTGGTACTCCAGCACGTCCGCACCGGTAACGata > 7:188907/1‑113 (MQ=255) gTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACgg < 7:20657/117‑1 (MQ=255) gTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACgg > 8:20657/1‑117 (MQ=255) tCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGatat < 8:151739/109‑1 (MQ=255) tCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGatat > 7:151739/1‑109 (MQ=255) cggcggGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat < 7:321041/104‑1 (MQ=255) cggcggGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat > 8:321041/1‑104 (MQ=255) gacgggtaaaacaaaaGGCCGTTTCACGCACCGGGACCTGGCTGCCTTCCAGCGCC‑TGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTACGGATATCGACGGTTGAGTTTTCCGAATATGCTTTTCCGGGGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGgcg < 4:239440/262‑1 (MQ=255) ggTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCCCCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAACGCTCCATGGCGGGACACGCCGCCACGATTTTTTGCCCCTCTTGGGGCCGTTTCCCgggccga > 4:457557/1‑269 (MQ=255) ataCACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat < 8:1075338/96‑1 (MQ=255) ataCACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat > 7:1075338/1‑96 (MQ=255) taCACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGCATATCAACGGCCCGACGCAGAATACGCACGCGCTCCATGGCGGTCATCAACGCCCAACTTTTTTGCCCATTCTGGGCGATTTTCACGGCGCGATCGa > 2:237228/1‑274 (MQ=255) acacAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTAGTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAg > 7:229712/1‑136 (MQ=255) acacAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTAGTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAg < 8:229712/136‑1 (MQ=255) cacaAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGa > 8:166144/1‑98 (MQ=255) cacaAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGa < 7:166144/98‑1 (MQ=255) acaAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTg < 8:901711/148‑1 (MQ=255) caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACt < 5:576271/66‑1 (MQ=255) caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACt > 6:576271/1‑66 (MQ=255) caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAg < 6:545243/70‑1 (MQ=255) caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg < 5:554398/134‑1 (MQ=255) caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTc < 6:1095342/139‑1 (MQ=255) aaGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGCTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTAATCGCCGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGGCTGGATCGCAATCCtg > 1:110755/1‑274 (MQ=255) aGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCACCGGCCCGACGCAGAATACGCGAACGCTCCATGGCGGTCATCGCCGCCCAGATTTTTTGCCCCTGCTGGGCCCTTTTCACGGCCCTACCCACATc > 4:155786/1‑272 (MQ=255) aGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGATGGCTTCATGGCGGTGATCGACGCCCAGGTTTTTTGCCCCCGATGGTCGCTTGTCACGTCGTGATGGGCATCCTc > 2:89078/1‑274 (MQ=255) aGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGGCGCGATCGACATCCt < 4:509344/273‑1 (MQ=255) aGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCGCCATGGCGGTCATCGACGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGGCGCGACCGCCATcccc > 4:465194/1‑272 (MQ=255) ggCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTNACGCAGAATATCAAc > 3:26986/1‑154 (MQ=255) cTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt > 6:604845/1‑91 (MQ=255) cTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt > 8:618219/1‑91 (MQ=255) cTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt < 5:604845/91‑1 (MQ=255) cTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt < 7:618219/91‑1 (MQ=255) gCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGcc < 1:26986/154‑1 (MQ=255) ttCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGCCCAGATTTTGTGCCCCTGCTGGGCGCTTTTCACGGCGCGATCGACATCCTCGCGCCCGGCGGCCTGCACGGTCGCCAGCACg < 2:201762/274‑1 (MQ=255) tCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGt > 7:124038/1‑101 (MQ=255) tCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGt < 8:124038/101‑1 (MQ=255) tCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACgg > 8:830062/1‑148 (MQ=255) ccAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACg < 8:616173/146‑1 (MQ=255) ccAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACg > 7:616173/1‑146 (MQ=255) ccAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGc < 7:830062/148‑1 (MQ=255) acgcC‑GGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg > 8:399940/2‑95 (MQ=255) acgcC‑GGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg < 7:399940/94‑1 (MQ=255) acgcC‑GGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTg > 7:876817/2‑108 (MQ=255) acgcC‑GGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTg < 8:876817/107‑1 (MQ=255) acgcC‑GGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt > 5:162621/2‑120 (MQ=255) cgcC‑GGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGttt > 7:116179/1‑98 (MQ=255) cc‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAat > 6:79495/1‑72 (MQ=255) cc‑GGCATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAat < 5:79495/72‑1 (MQ=255) gggATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGa > 5:794005/1‑88 (MQ=255) gggATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGa < 6:794005/88‑1 (MQ=255) gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCgg > 5:293060/1‑80 (MQ=255) gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCgg < 6:293060/80‑1 (MQ=255) gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt < 5:602856/113‑1 (MQ=255) gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt > 6:602856/1‑113 (MQ=255) aTCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTCTCCGAATATGCTTTTCCGGTGTCCAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACTCAGAATCCGCGAGCGCTCCATGGCGGTCATCGCCGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGGCGCGGTCCACCACCTCCGCCCCGTCCGCCGGCCCGGGCCCCAACACTTTCCCCGTGGcccg > 4:522444/1‑273 (MQ=255) | CACGCCCGGCAACACGTTAAATACGCCGTCCGGCAGGCCCGCTTCGCTGTAAATTTCAGCCAGCTTTAACGCGGTAAGCGGGGTAACTTCGCTCGGTTTGAAAATCATTGCGTTGCCTGCCGCCAGCGCCGGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCC‑GGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGCCCGACGCAGAATACGCGAGCGCTCCATGGCGGTCATCGACGCCCAGATTTTTTGCCCCTGCTGGGCGCTTTTCACGGCGCGATCGACATCCTCGCGCCCGGCGGCCTGCACGGTCGCCAGCACGTTACCGTTGGCCGG > NC_000913/328110‑328649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |