| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,707,320 | A→T | 5.5% | intergenic (‑196/+2) | lepA ← / ← rseC | back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,707,320 | 0 | A | T | 5.5% | 664.6 / 20.8 | 181 | intergenic (‑196/+2) | lepA/rseC | back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component |
| Reads supporting (aligned to +/- strand): ref base A (101/70); new base T (5/5); total (106/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-02 | |||||||||||
Only 100 of 183 total aligned reads displayed.
TCGATTTACCGTGGTCAATGTGAGCTATGATCGAAAAGTTACGTATATTCTTCATAAAGTATGATTATTGTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGCTAAGCAGGCTGCCTT > NC_000913/2707070‑2707590 | tCGATTTACCGTGGTCAATGTGAGCTATGATCGAAAAGTTACGTATATTCTTCATAAAGTATGATTATTGTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGANACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCNTCGCATTTGTTATGCAAAATGCAAAAAAGCNNATGAAAACAATGGCCTGAGTCttttca > 3:76183/1‑269 (MQ=255) tcttcATAAAGTATGATTATTGTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACCTTGAGGCGAGGAAATGTTCATACCTTATGGATTGTGGTATCTGGAAACGTCC‑CGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCAACAGGCCAGGCGGCCATGCCACGCTTAAgatta > 2:361161/1‑272 (MQ=255) aTGATCATTGTGCCTTAATGCCCGGTTAACCACGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGCATTAATAGAAACGCCGCATTCTACACTACAACATTGCGGCGAGGAAAGGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTGGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGTTCGCGTCTTCCGAAGATGATTCAAATCGCACAAGGCCAAGCGGAAATGCCCCGCTTAAGATGATCGGTAGACAta > 4:55965/1‑274 (MQ=255) tgtgCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg > 2:207801/1‑274 (MQ=255) gtgCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATAATCGGTTGCCATTCTGCCAggg > 2:60334/1‑275 (MQ=255) gtgCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCCCGCGTCTTCCGAAGATGTTTCAACTCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATAATCGTTTGCCATTCTGCCCggg > 4:470643/1‑275 (MQ=255) aTGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCCCGCGTCTTCCGAAGATGTTTAAAATCGCACCAGGCCAGGCGGCCAGTCCACGCTTAAAGTTGTCGGTTGCCCTTCTGCCCGGGGAGCaaa > 4:454274/1‑274 (MQ=255) gTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaa < 5:261331/148‑1 (MQ=255) cTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa < 7:741662/148‑1 (MQ=255) ttcgtggCTTAACGTTTGTATTAATCGAAACGCCGCAGTCTACACTACAACATGGAGGGGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTGATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAGGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAAccc < 2:29403/268‑1 (MQ=255) gAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTc > 7:133824/1‑148 (MQ=255) aaCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGCTGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGACCGCTTGCCATTCTGCCCGGCCCGCAAACTTACGCGAGTACCCGCGCGCAATCAGGAACCCGCCAATaccc > 4:201284/1‑273 (MQ=255) aaCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACCAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGAAGCAAACTTACGCGAGTAGCCGCGCCCAATCAGGAACCCGCCAAtcacc > 4:258826/1‑270 (MQ=255) aaCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATacca > 2:331086/1‑274 (MQ=255) tGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCCCGCAATCAGGAACCCGCCAATACCACCGAGGa > 4:401403/1‑275 (MQ=255) tAATAGAAACCCGGGATGCAAAACTAGAACAGTGGGGTGAGAAAATGTTCATACCGAATGGATTGGAGGATCTGAAAACGTCC‑CGCATTGTTTATGCAAAATGAAGCAAAGCCAGTAGAATCACTGACTGGCGTCTTCCGAGGATGATGCAATACGCAAAAGGCCAGGCGGAAGTGCCACGATTAAGATGATCGGTTGACATTCGGCCCGGGCAGCAAACGTCCGAAGGTAGCCGCGCACAATCAGGAACCCGCCAATACCACCGAGGAT‑CGca < 4:361160/274‑1 (MQ=255) aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGcac > 2:394305/1‑274 (MQ=255) aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGcac > 2:444789/1‑274 (MQ=255) gAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa > 6:1106728/1‑119 (MQ=255) gAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa < 5:1106728/119‑1 (MQ=255) aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGc > 6:1047749/1‑122 (MQ=255) aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc > 6:382956/1‑129 (MQ=255) aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc < 5:382956/129‑1 (MQ=255) aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCa > 4:83912/1‑167 (MQ=255) aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCt > 2:184905/1‑199 (MQ=255) aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTCCGCGAGTAGCCGCGCGCCACCCGGAACCCGCCATAACCCCCGAGGAC‑CACACcccaa > 4:154946/1‑273 (MQ=255) ccgcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGc < 2:83913/167‑1 (MQ=255) ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAgg > 8:1036877/1‑146 (MQ=255) ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAgg < 7:1036877/146‑1 (MQ=255) acaacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa > 5:814134/1‑99 (MQ=255) acaacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa < 6:814134/99‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGc > 8:183666/1‑103 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGc < 7:183666/103‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGca < 2:133253/249‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAC‑CCCACCACATAATGCCGCGACCTCAGaacc > 4:491006/1‑273 (MQ=255) aacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcg > 4:529572/1‑209 (MQ=255) acaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAt > 7:400825/1‑148 (MQ=255) caTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCACTCCGGAACCTGCCACTACAACCGAGACT‑CGCACCACATAATGCCGCGACgcccaaaacaca > 4:392709/1‑264 (MQ=255) tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAgg > 7:363405/1‑132 (MQ=255) tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAgg < 8:363405/132‑1 (MQ=255) aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGCA‑CGCACCACATAATGCGGCGACGTCAGAAGCAAAGAGTa > 2:431046/1‑275 (MQ=255) aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTAAGAAGCAAAGAGTa > 1:65737/1‑275 (MQ=255) aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt > 6:49832/1‑110 (MQ=255) aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt < 5:49832/110‑1 (MQ=255) aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTCCGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGCTACGC‑CCCCATANNNCAGCGACGGCAGAAGCaaacaaaaacaga > 3:157582/1‑265 (MQ=255) aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATac > 1:128461/1‑222 (MQ=255) aaTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatg > 5:621039/1‑148 (MQ=255) aTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatga < 8:400825/148‑1 (MQ=255) tGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatgat > 7:243564/1‑148 (MQ=255) tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa > 7:1128781/1‑76 (MQ=255) tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCt < 8:1130352/89‑1 (MQ=255) tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCgg < 6:621039/148‑1 (MQ=255) cATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGt < 8:243564/148‑1 (MQ=255) aCCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACg > 4:143239/1‑241 (MQ=255) tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTCAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCCCATAAAACTGCGACGCCAGAAGCAAAAGATAGCCGAAATACCGAACCGaaaaa > 4:511246/1‑274 (MQ=255) tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAg < 2:143240/241‑1 (MQ=255) aTGGATTGTGGTATCTGGAGACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATCCCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCCGAAGCAAAGAGTAGCTGAAATAGCGAAGCGCTAAg > 2:11692/1‑274 (MQ=255) aTGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc > 5:210704/1‑148 (MQ=255) aTGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGGA‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCCGAAATAGCGAAGCGAAAAgg > 2:28438/1‑275 (MQ=255) tgGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTc > 3:136077/1‑66 (MQ=255) ggTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATccc > 4:128560/1‑275 (MQ=255) tATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt > 5:1023223/1‑81 (MQ=255) aTCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGt < 1:136077/66‑1 (MQ=255) aTCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAgg < 8:768049/95‑1 (MQ=255) aTCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAgg > 7:768049/1‑95 (MQ=255) cTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCa > 6:603433/1‑103 (MQ=255) cTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCa < 5:603433/103‑1 (MQ=255) gAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACaa > 5:710014/1‑88 (MQ=255) gAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACaa < 6:710014/88‑1 (MQ=255) gAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCa > 5:584171/1‑106 (MQ=255) aaaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCGGGCGGCAATGCCACGCTTa < 5:1066508/111‑1 (MQ=255) aaaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCGGGCGGCAATGCCACGCTTa > 6:1066508/1‑111 (MQ=255) aaCTTCTTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTGGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCATGGCCACGCTTAAGATGGTCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAgcggcg < 2:81194/275‑1 (MQ=255) aaCGTCTTCGCATTTGTTATGCAAAATGCAACAAAGCCAGGGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCCCAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAgcggcg < 2:454275/275‑1 (MQ=255) aaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTa < 6:210704/148‑1 (MQ=255) aaCATCCCCGCATTTATTATCCAAAATGAAACAAAGCCAGTGAAATCACTGGCGCGCGTCTTCCGAAGATGTTTCAAACCGAACAAGGCCAGGCGGCAATGCCACACTTAAGAGGATCGGTTGCCATTCTGCCCGTGCAGCAACCTTAAGCGAGTACCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAgcggcg < 1:44844/275‑1 (MQ=255) cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACata < 2:521749/274‑1 (MQ=255) tCGCATTTTTTATGCAAAATGCAACAAAGCCAGTTAAATCACTGGCTCGGGTCTTCCGAAGATTTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTCCGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACAtata < 2:55966/275‑1 (MQ=255) tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCg < 6:577515/112‑1 (MQ=255) cGCATTTTTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAgcggcg < 2:484258/267‑1 (MQ=255) cATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGc > 8:1043522/1‑148 (MQ=255) tttGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGc > 8:575768/1‑77 (MQ=255) gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAAt > 6:950302/1‑148 (MQ=255) cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACtt < 8:882451/126‑1 (MQ=255) cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACtt > 7:882451/1‑126 (MQ=255) aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCa < 7:1043522/148‑1 (MQ=255) aaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatga < 8:642610/92‑1 (MQ=255) gCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTaa < 5:968297/84‑1 (MQ=255) acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcg < 6:469773/120‑1 (MQ=255) acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcg > 5:469773/1‑120 (MQ=255) acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAc > 7:784329/1‑119 (MQ=255) acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAc < 8:784329/119‑1 (MQ=255) caaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGCTAAg > 2:237469/1‑272 (MQ=255) aaaGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCaa > 8:1020909/1‑135 (MQ=255) aaaGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCaa < 7:1020909/135‑1 (MQ=255) aGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAgg > 7:632224/1‑138 (MQ=255) cAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAc < 8:111578/112‑1 (MQ=255) gTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCAGAAATAGCGAAGCGATAAGGAATAAACCCACCAGCGGCGAAATGTAAACCAGTAAAGCGGAGCTAAGCAGACCGCCt > 4:296485/1‑274 (MQ=255) gTGAAATCACTGGCTCGCGTCTTCCGAAGAGGTTTCAAATCGCACAAGGCCAGGCGGCAATGCAACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGCTAAGCAGGCTGCCtt < 2:212329/275‑1 (MQ=255) tGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATaccac > 6:917149/1‑148 (MQ=255) | TCGATTTACCGTGGTCAATGTGAGCTATGATCGAAAAGTTACGTATATTCTTCATAAAGTATGATTATTGTGCCTTAATGCCCGGTTAACCAGGCTTTTAGAAGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGAGGAT‑CGCACCACATAATGCTGCGACGTCAGAAGCAAAGAGTAGCTGAAATAGCGAAGCGATAAGGAATAATCCCACCAGCGGCGACATATAAACCAGTAATGCGGAGCTAAGCAGGCTGCCTT > NC_000913/2707070‑2707590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |