Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,863 A→G 12.9% intergenic (+30/+16) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8630AG12.9% 173.7 / 16.4 63intergenic (+30/+16)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base G (4/4);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-02

ACAGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGT  >  NC_000913/3171590‑3172114
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                           
aaaggggaggggGGTAGACGTCACGCGGNATGCTGATAATTGCATCGTGAGGGGNAACGGCCCCGGTGGGACACCAGAGGCACTGGCGAGAATTGGCGAACGCTTCTATCGCACACCCGGACAAACCGCTACCGGNAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                                                                                                                                                                                             <  3:88719/272‑1 (MQ=255)
     gCAGCGTCGTAGACGTCACCCTGAATGCTGCTACTTTCATCTTGAGGGATAACGGCCCCGGTGTGACACCAGGGGCACTTGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCGTCCGGCAGCGTGCTTGGGCTATCGATTGTCCAGCGCTTCGCCAAATTGCATGGCATGAGTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGGGCCGTTAAAACGACTCTCa                                                                                                                                                                                                                                                       <  4:166728/275‑1 (MQ=255)
                         cgtaaTGGTGAGAAATTTAGCGGGGGGGATAACGGCCCCGGTGTGAAACCCGAGGCAATGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                                                                                                                    <  4:318383/271‑1 (MQ=255)
                                      aTTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAACTTTCATGGCATGAATGTTGAACTTGGGAATTCGGACAAAGGTGGATTTGAGTGGAAGGTANGCTGGTAACTTCTCTGTATATGAAGACGGTTAAAAACACACACAAAGATTTGATTAATAGACAAATATaaaacctac                                                                                                                                                                                                                      >  3:133743/1‑269 (MQ=255)
                                                     aTAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                                                                                                                                                                                             >  2:486720/1‑221 (MQ=255)
                                                     aTAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGa                                                                                                                                                                                                                                                             <  4:486719/221‑1 (MQ=255)
                                                               cGGGGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                                                                                                                              <  2:338689/274‑1 (MQ=255)
                                                                            gagGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                                                                                                                                                                                            >  3:35522/1‑199 (MQ=255)
                                                                             gtgCAGTGGTGCGGATGGGCCAACGCTTCGTTCGCCCACCCGGACAAACCGCTACCGGCAGCGGGTTTGGGCTATCGATTGTCCAGCGAATCGGCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTGATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAACAGACAAATACcggg                                                                                                                                                                               <  4:9560/273‑3 (MQ=255)
                                                                               gCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                                                                                                                              <  2:37686/274‑1 (MQ=255)
                                                                                cACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACtctc                                                                                                                                                                                                                                                        <  1:35522/199‑1 (MQ=255)
                                                                                  cTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                                                                                                                           <  2:351915/274‑1 (MQ=255)
                                                                                                                                      gCAGCGGGCTTGGGCTATCGCTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGATGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGGGCCGTTAAAACGACTCTCATAGATTTTCCTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCCCAACCCAGACAAATACCGAGCGAGAATACATATCCCCAAAACCCGAAACTTTccacca                                                                                                                                         >  4:190686/1‑252 (MQ=255)
                                                                                                                                          cGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                                                                                                                                     <  2:190687/256‑1 (MQ=255)
                                                                                                                                                       tCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                                                                                                                                                                                    >  6:134752/1‑132 (MQ=255)
                                                                                                                                                       tCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                                                                                                                                                                                    <  5:134752/132‑1 (MQ=255)
                                                                                                                                                                  gCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                                                                                                                                         <  5:162181/132‑1 (MQ=255)
                                                                                                                                                                  gCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                                                                                                                                         >  6:162181/1‑132 (MQ=255)
                                                                                                                                                                        cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                                                                                                                                                                                                   >  8:521555/1‑148 (MQ=255)
                                                                                                                                                                          ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                                                                                                                                      <  5:806767/111‑1 (MQ=39)
                                                                                                                                                                          ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaa                                                                                                                                                                                                                 >  6:242301/1‑148 (MQ=255)
                                                                                                                                                                          ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTCCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                                                                                                                                      >  6:806767/1‑111 (MQ=38)
                                                                                                                                                                              aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                                                                                                                                           >  6:29914/1‑134 (MQ=255)
                                                                                                                                                                              aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                                                                                                                                           <  5:29914/134‑1 (MQ=255)
                                                                                                                                                                                tGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcc                                                                                                                                                                                                           <  7:521555/148‑1 (MQ=255)
                                                                                                                                                                                       catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAct                                                                                                                                                                                                                                                           <  8:373996/93‑1 (MQ=255)
                                                                                                                                                                                       catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAct                                                                                                                                                                                                                                                           >  7:373996/1‑93 (MQ=255)
                                                                                                                                                                                       catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                                                                                                                                         >  8:824531/1‑111 (MQ=255)
                                                                                                                                                                                       catgAATGTTGAATTTGGGAAGGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                                                                                                                                         <  7:824531/111‑1 (MQ=255)
                                                                                                                                                                                         tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                                                                                                                                                                                              >  8:196494/1‑104 (MQ=255)
                                                                                                                                                                                         tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                                                                                                                                                                                              <  7:196494/104‑1 (MQ=255)
                                                                                                                                                                                                    tttGGGAATGCGGAACAAGGTGGATTTGTGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                                                                                                                                                                                   <  6:1041921/88‑1 (MQ=255)
                                                                                                                                                                                                    tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                                                                                                                                                                                   >  5:1041921/1‑88 (MQ=255)
                                                                                                                                                                                                          aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                                                                                                                                      >  6:675962/1‑79 (MQ=37)
                                                                                                                                                                                                          aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                                                                                                                                      <  5:675962/79‑1 (MQ=37)
                                                                                                                                                                                                           gtGCGGAACAAGGAGGATTTGGGGCGAAGGTATGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                                                                                                                                        <  4:463215/171‑1 (MQ=255)
                                                                                                                                                                                                           aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                                                                                                                                                                                <  5:242301/148‑1 (MQ=255)
                                                                                                                                                                                                                gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtttt                                                                                                                                                                                                                                               >  5:380204/1‑80 (MQ=255)
                                                                                                                                                                                                                gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtttt                                                                                                                                                                                                                                               <  6:380204/80‑1 (MQ=255)
                                                                                                                                                                                                                             ttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                                                                                                                                            >  7:634766/1‑86 (MQ=255)
                                                                                                                                                                                                                             ttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                                                                                                                                            <  8:634766/86‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                                                                                                                                             >  8:345643/1‑93 (MQ=255)
                                                                                                                                                                                                                                     aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                                                                                                                                             <  7:345643/93‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                                                                                                                                                                                     >  8:1014326/1‑101 (MQ=255)
                                                                                                                                                                                                                                     aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                                                                                                                                                                                     <  7:1014326/101‑1 (MQ=255)
                                                                                                                                                                                                                                          aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                                                                                                                                             >  6:991333/1‑88 (MQ=255)
                                                                                                                                                                                                                                          aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                                                                                                                                             <  5:991333/88‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCAttg                                                                          >  2:513255/1‑211 (MQ=255)
                                                                                                                                                                                                                                                      tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCAttgcttg                                                                      <  4:513254/211‑1 (MQ=255)
                                                                                                                                                                                                                                                           gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                                                                                                                           >  1:138636/1‑153 (MQ=255)
                                                                                                                                                                                                                                                            tCTAAGAGCGCCATTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGt  <  1:133743/273‑1 (MQ=255)
                                                                                                                                                                                                                                                             cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                                                                                                                                        <  6:628479/122‑1 (MQ=255)
                                                                                                                                                                                                                                                             cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                                                                                                                                        >  5:628479/1‑122 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGa                                                                                                                                                     <  8:298073/123‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGa                                                                                                                                                     >  7:298073/1‑123 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcc                                                                                                                                                                                                                      >  7:48196/1‑58 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcc                                                                                                                                                                                                                      <  8:48196/58‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                                                                                                                                                                                            >  7:953982/1‑68 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                                                                                                                                                                                            <  8:953982/68‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAg                                                                                                                                                                                           <  7:798748/85‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAg                                                                                                                                                                                           >  8:798748/1‑85 (MQ=255)
                                                                                                                                                                                                                                                               aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga                                                                                                                       <  3:138636/153‑1 (MQ=255)
                                                                                                                                                                                                                                                                tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt                                                                                                                                                                        <  7:951531/103‑1 (MQ=255)
                                                                                                                                                                                                                                                                tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt                                                                                                                                                                        >  8:951531/1‑103 (MQ=255)
                                                                                                                                                                                                                                                                 gagaGCCGTTACAACGTCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                                                                                                                               <  7:828043/95‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                                                                                                                               >  8:828043/1‑95 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                           
ACAGGGCAGCGTGGTAGACGTCACGCTGAATGCTGATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATTGGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGT  >  NC_000913/3171590‑3172114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: