| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,180,506 | A→C | 8.5% | E29A (GAG→GCG) ‡ | ygiC → | ATP‑Grasp family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,180,506 | 0 | A | C | 8.5% | 394.8 / 31.6 | 133 | E29A (GAG→GCG) ‡ | ygiC | ATP‑Grasp family ATPase |
| Reads supporting (aligned to +/- strand): ref base A (62/58); new base C (6/5); total (70/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-04 | |||||||||||
Only 100 of 135 total aligned reads displayed.
ACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTG > NC_000913/3180233‑3180762 | acgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAg < 1:40453/275‑1 (MQ=255) agcccagccAGGCCGAACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGc < 2:308494/275‑1 (MQ=255) ccGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACTCCCTGTACGGCGAGGCGTACTGGAGTGAAGATGGTTACTAAAAg > 2:111731/1‑273 (MQ=255) tACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAACTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAACGGGTCAGTATTAACGAGCGCCCGGACTGGGGGAAGAAAGCCAACGAATACAGTTTAAATTTTCAACCAATGTACGGCGAGCCGTACGGGGGTGAAGGTCCTAACACCAAGTTACCCCCCGACCA‑gg > 4:485443/1‑275 (MQ=255) tACCGAAAAGGGCAAAGGCACCAAAACCGGCGACAACCACTACCGTGACCCGGGGCGGTTTTGGGGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGccc < 4:408777/272‑1 (MQ=255) aCGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGACAGTATTACCCAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATAAGGGTTCAATTTTCACCACATGTACGGGCGGCCGTACGGGTGTGAAGATGATTAATAAAAGTTGCCCCTCCCCCA‑GGGTTaaaa > 4:477442/1‑274 (MQ=255) ggCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCGCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGCGTCAGTATTACCGAGCGCCCGGACTGGCGGGAGAAAGCCCACGAAAACGGTTTCAATTTTCACACCATGTACGGGAGGCAGGACTGGTGTGGAGATGATGAANNNAAGTTTACACTCGCCCAGGGTTGaaatc > 3:148136/1‑272 (MQ=255) cAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGc > 2:318562/1‑237 (MQ=255) ggCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTtac < 4:318561/237‑1 (MQ=255) caccacTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCa > 2:280446/1‑275 (MQ=255) tACCGTTACCCGTGGCGGGTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAt < 2:99879/275‑1 (MQ=255) ggCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgt > 2:262418/1‑274 (MQ=255) gatGCCAAACAAAGAACTATGCAACGTAGTGCAACCGGTACTTCTTCTCGTTCAATGGGTGGCCGATACAGTTGGAAAGAGACAGTATTACCGAGCGACAGGATTGGTGCAAGAAAGCCTACGAATACGGTTTAAATTTTAACAACATGTACGGCGATCCGTGATGGTGTGAAGATGCTTACTACAAGTTGACCAACGCACA‑GGTTGAAAAGGAGGAAGGAGGCACCGCCGAATTGCACCAGATGGTCCTGAAAGGAGTGAGAAAAGAGAACGca > 4:97362/3‑274 (MQ=255) aGCACTATGCGGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATGCCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGCGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGcc > 8:100913/1‑148 (MQ=255) aCTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAg < 7:157095/143‑1 (MQ=255) aCTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAg > 8:157095/1‑143 (MQ=255) aCTACGCAGCGTGGTGCAACCGGTACCTCTTCTCGTTCAGTGGGGGGCTGATACCGATGGAAAGAGTCATGATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGACTACGGTTTCAATTTTAACACCATGTACGGCGAGCCGTa < 7:966290/148‑1 (MQ=255) aTGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTg < 7:339114/148‑1 (MQ=255) tGCAGCGTAGTTCAACCGGTACCTCTTCTCGATCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTAAATTTTCACACAATGTACGGCGAGCCGTACTGGTGTGAAAATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAAATGCACCAGATGTACCTGAAAGTGGGTGAAAAAGGGACCGACCACAATCAGCTCATGAACa > 4:415965/1‑275 (MQ=255) tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGCTTCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATTTGCCTGAAAGTGGTGGAAAACGTGAGCGCCCGCGATGACCTGATGACCa > 4:192732/1‑275 (MQ=255) cAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGc < 5:642355/138‑1 (MQ=255) cAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGc > 6:642355/1‑138 (MQ=255) aGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCTACGAATACGGTTTCAATTTTCACACTATGTACGGCGAGCCGTACTGGTGTGAAGATTCTTACTACAAGTTGACCCTCCCCCA‑GGTTGGAAAGCTGGACGAATTCACCGCCGAACGGCACCAGAGGTGCCTGAAAGTAGGTGAAAAAGTGATCCCCAGCGTGTCGCTTATGACCaact > 4:197335/1‑273 (MQ=255) cGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAATAAGTGATCGCCAGCGATGAGCTGATGACCAAATTc > 2:253823/1‑275 (MQ=255) tAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg < 4:262417/275‑1 (MQ=255) tAGTGCAAACGGTACCCTTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGGAGTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTTACACCATGTACGGCGAGCCGTACTGGTGGGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg < 4:111730/275‑1 (MQ=255) aGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATCCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGGGGAAAAAGTGACCGCCAGCGATGAGCGGATGACCAAATTCCg > 4:254748/1‑274 (MQ=255) aGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGa > 7:149346/1‑130 (MQ=255) tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg > 2:67347/1‑272 (MQ=255) tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg > 2:480020/1‑272 (MQ=255) tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCAt > 4:175056/1‑275 (MQ=255) ctctTTTCGTTCCATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAacacac < 1:148136/275‑1 (MQ=255) cttctCTTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa > 6:606801/1‑120 (MQ=255) cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa < 5:606801/120‑1 (MQ=255) cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACAcc > 2:363661/1‑274 (MQ=255) ttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGAATTCCAAAACACACCTg > 2:8435/1‑275 (MQ=255) tctcGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGc < 5:701755/133‑1 (MQ=255) tctcGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGc > 6:701755/1‑133 (MQ=255) cGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatgg > 8:328768/1‑115 (MQ=39) cGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatgg < 7:328768/119‑5 (MQ=39) tCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatgg < 7:531472/116‑5 (MQ=39) tCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatgg > 8:531472/1‑112 (MQ=39) tggTGGCTGTTACCGATGGAAAGGGTCAGTATTACCGAGCGCCCGGACTGGCGTGGGAAAGGCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGGGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg < 4:67346/274‑1 (MQ=255) gggTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTgcg < 4:363660/275‑1 (MQ=255) cTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTc < 2:175057/275‑1 (MQ=255) tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa < 5:361632/100‑1 (MQ=255) tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa > 6:361632/1‑100 (MQ=255) aTACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGc < 5:176327/148‑1 (MQ=255) aTACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCt > 4:559497/1‑185 (MQ=255) gaCCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTCCTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCt < 7:95315/147‑1 (MQ=255) aCCGATGGAAAGAGTCAGTATTAGCGAGCGCCCGGGCTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTTACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACTCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGAACGCCAGCGATGAGCTGATGACCACATTGCGCATTCCAAAACACACCTGGAGGTTTGTGCGCCAGTCATgga > 1:51511/1‑274 (MQ=255) cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaaa < 2:559498/185‑1 (MQ=255) cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcat < 7:854492/96‑3 (MQ=38) cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcat > 8:854492/1‑94 (MQ=38) gATGGAAAGAGTTAGTATTACCAAGCGACCGGACCTGCGTGTGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGa < 2:415966/275‑1 (MQ=255) agagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatg < 8:949115/89‑4 (MQ=37) agTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGc > 2:497060/1‑199 (MQ=255) tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACAAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCa > 1:107704/1‑275 (MQ=255) cAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGgaaga < 8:159888/138‑1 (MQ=255) cAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGCAGAAGTAACCGCCGAACTGCACCAGATGTGCCTGAAAGGGGTGGAAAAAGTGATCGCAAGCGATGAGCTGATGACCCAATTCCGCATTCCAAAAACCACTTGGAGTTTTGTGCGCCAGTCTTGGCTGCCCCACCAGcaa > 4:301527/1‑272 (MQ=255) aGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATg < 4:497059/199‑1 (MQ=255) cGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc > 7:454670/1‑148 (MQ=255) cgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTCCTGGTGTGAAGATGCTTACTACACGTTGCCCCTCACCCA‑GGTTGAACAGCTGGAAGAAGTCACCGACAACCTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGCTCGACAGCGATGAGCTGATGACCCAAGTACGCATTACAAAACACACCTGGGGTTTTTTTCGACAGTCATGGCTGACGCACCAGCCATCGCTTTAtcagct > 4:307511/1‑270 (MQ=255) cgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAACAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTcgcg > 2:512360/1‑275 (MQ=255) cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa < 4:102491/64‑1 (MQ=255) gACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGc < 6:1108631/112‑1 (MQ=255) gCGTGAGAAAGCCCACGAATACGGTTTCAAGTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCG‑‑GAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg < 2:477443/275‑1 (MQ=255) cGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGc < 7:859560/106‑1 (MQ=255) tGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtg > 6:204526/1‑148 (MQ=255) gagaAAGCCCACTAATACGGTTTCAATTTTCACACCATGTACGGCGAGTCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGGCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATTAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg < 1:108227/273‑1 (MQ=255) gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCt > 6:956875/1‑104 (MQ=255) gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTCACCCTCGCCCA‑GGTTGAAATGCt < 5:956875/104‑1 (MQ=255) aaaGCCCNCGAATCAGGTTTCAATTTGCAAACCATGTACGGCGAGCCGTACTGGTGGGAAGATGCTTACTAAAAGTTGACCCTCGCCCA‑GGTGGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGa < 4:512359/275‑1 (MQ=255) aaaGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTgg < 6:799797/54‑1 (MQ=255) cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTg < 8:198604/117‑1 (MQ=255) cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTg > 7:198604/1‑117 (MQ=255) aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTg > 5:643083/1‑95 (MQ=255) cGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTg < 6:666805/94‑1 (MQ=255) cGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTg < 5:204526/148‑1 (MQ=255) gAATACGGTTTTAATTTTCCCACCATGTACGGGGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTAACCCTGGCCCA‑GGTGTAAAAGCTGGAAGAAGTAACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTgg < 4:260107/275‑1 (MQ=255) gTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTg > 6:518204/1‑140 (MQ=255) tttCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATg > 7:179068/1‑47 (MQ=255) tCAATTTTCACACCATGTACAGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGACGAAGTCACCGCCGAACTGCCCCAGATTTGCCTGAAAGTGGTGGAAAACGTAATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTATTCGCCAGTCATTGCTGACGCCCCATCCATCGCTTTATTCCCTTCTTGACCTGGCGTTGGATGGCACGGGTGACCCTaaa > 4:347202/1‑275 (MQ=255) aTTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgg > 5:718406/1‑123 (MQ=255) ttCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGACCCTAAACTTCTg > 1:108059/1‑275 (MQ=255) cacCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCa > 8:430839/1‑83 (MQ=255) cacCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt < 7:272343/117‑1 (MQ=255) cacCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt > 8:272343/1‑117 (MQ=255) ccATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGa > 8:561116/1‑88 (MQ=255) ccATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 8:15924/1‑148 (MQ=255) gTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCaaa < 7:15924/148‑1 (MQ=255) tACGGCTCGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaa > 6:866531/1‑104 (MQ=255) tACGGCTCGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaa < 5:866531/104‑1 (MQ=255) tACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatg < 5:687171/141‑1 (MQ=255) tACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatg > 6:687171/1‑141 (MQ=255) cGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtg < 8:941863/91‑1 (MQ=255) cGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtg > 7:941863/1‑91 (MQ=255) cGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt > 8:246108/1‑105 (MQ=255) cGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt > 8:241601/1‑105 (MQ=255) cGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt < 7:241601/105‑1 (MQ=255) | ACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCA‑GGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTG > NC_000913/3180233‑3180762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |