Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,231,473 T→G 5.8% intergenic (+234/‑192) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,231,4730TG 5.8% 245.1 / 6.9 76intergenic (+234/‑192)ygjK/fadHalpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base T (33/37);  new base G (2/3);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

GTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTC  >  NC_000913/3231203‑3231738
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                         
gTATATTTTGAATAACGATTTTTTCCGTAAGCAATAATTTGCAAATGCTGGCGAATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATc                                                                                                                                                                                                                                                                       <  4:67682/275‑1 (MQ=255)
      gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAACCGCCCTACATGTGTTTTCCCGTAGGTCGGATAAGACGCGACCAGCGTCGCATCCGGCATCTGCCCCGCGCCACTGGCGGATGCGGCGTGGCCGCCTGATCCGcccg                                                                                                                                                                                                                                                                 >  4:230142/1‑274 (MQ=255)
        tgtATAACGCTTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTTTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCTCGCCACTGGCCGATGCGGCGTGGACGCCTTATCCGCCCtcc                                                                                                                                                                                                                                                               >  2:67683/1‑273 (MQ=255)
         gtATAACGAATTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGGGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTAc                                                                                                                                                                                                                                                               <  2:332535/274‑1 (MQ=255)
           ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCTTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAATGCCTTATCCGCCCTACATGTGCGTTCCCGTAGGTCGGATAATACGCGACACGCGTCGCATCCGGCATCTGCACCGCCCCACCGGCGACTGCGGCGTGTACTACTTACCCGTCCTACac                                                                                                                                                                                                                                                             >  4:263965/1‑273 (MQ=21)
           ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGCTGCGGCGTGGACGCCTTATCCGCCCTAcct                                                                                                                                                                                                                                                             >  2:253889/1‑272 (MQ=255)
           ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAt                                                                                                                                                                                                                                                             >  2:33313/1‑274 (MQ=255)
           ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGACGCGGACGCTGTATCCGCACTACAt                                                                                                                                                                                                                                                             >  1:42988/1‑274 (MQ=255)
               cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTACCCGCCCTACCTGTTCa                                                                                                                                                                                                                                                        >  2:57702/1‑275 (MQ=255)
                    ttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGCCGGATAAGACGCGACAAGCGTAGCATCCGGCATCTGCACCGTGCCACTGCCGGCACCGGCGTGGACCCCTTGTCCGCCCTTACTGGGCAATTcc                                                                                                                                                                                                                                                   >  4:52677/1‑275 (MQ=21)
                                                      aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATcc                                                                                                                                                                                                                 <  2:290209/275‑1 (MQ=255)
                                                      aTGCGGCGCAACCGCCATAACCTCCCTACATGGGAAATCCTCGTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATcc                                                                                                                                                                                                                 <  1:55042/275‑1 (MQ=255)
                                                       tGCGGCGTCAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATcc                                                                                                                                                                                                                 <  2:329799/274‑1 (MQ=255)
                                                       tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCCGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATcc                                                                                                                                                                                                                 <  2:449135/274‑1 (MQ=255)
                                                            cgTAAACGCCTTTACCGCCCTACATGGGCAATCCTCCGGGCCTGATAAACGGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGAGGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGtt                                                                                                                                                                                                           <  4:430427/275‑1 (MQ=255)
                                                                  cGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCAc                                                                                                                                                                                                      <  4:33312/274‑1 (MQ=255)
                                                                      ttATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCTCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGGAACCTGGGTAAAACGCGACAAGCGGCCCCACCCGCGTTCTCACCgggg                                                                                                                                                                                                 >  4:329968/1‑274 (MQ=25)
                                                                             cccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCCACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTCCACCGCGCCCCTGCCCGATGCGGCCTGGACGCCTTATCCCCCCTCCATTGCCAATTCCGGAACCTGGATAAACCGCCACAAGCCTCCCCTCCGCCTTTaccacctgccttctcc                                                                                                                                                                                           >  4:172668/1‑264 (MQ=21)
                                                                                        cAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGCCGGATGCGGCGTGGACGCCCTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCCCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATcc                                                                                                                                                                                >  2:215775/1‑274 (MQ=255)
                                                                                                ctGTCCGGATAAAACGCGGCAATCTGTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACaaa                                                                                                                                                                       <  2:417203/275‑1 (MQ=255)
                                                                                                       gATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCa                                                                                                                                                                <  2:329969/275‑1 (MQ=255)
                                                                                                       gATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCa                                                                                                                                                                <  2:66803/275‑1 (MQ=255)
                                                                                                          aaaaGGGGGCAAGCTTTCCAGCCGGCAACGGCACCGCGCCACGGGCGGATGCGGCGTGAAGGCCGTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGAGGCGACAAGCGTCGCAGCCGGCAGCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCAc                                                                                                                                                               <  4:253888/273‑1 (MQ=25)
                                                                                                                 ggCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGCTGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACACGCGTCGCATCCGGCATCTTCACCGCGCCACTGGCGGATGCGGCGTGGACCACTTAACCGCCCTACATCTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATTCACTCACAAACCCCGAACTCgccattt                                                                                                                                                      >  2:110647/1‑275 (MQ=37)
                                                                                                                                     catgtacccggcCAGTGGCGGTTGGGGCGTGAACGGCTTATCCGCCCTACATGTGGGTTGCCGGAGGTCGGATAAGACGCGACAAGCGTCGCCTCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                                                  <  4:57701/265‑1 (MQ=37)
                                                                                                                                           ccGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAAt                                                                                                                             <  2:52678/274‑1 (MQ=255)
                                                                                                                                                     ggCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCAAAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATc                                                                                                                  >  1:119017/1‑275 (MQ=255)
                                                                                                                                                       cGGATGGGGCGTGAAGCCCTTAACCGCCCTACAGGTGGGTTCACGGGGGTCGGATAAGACGCGACAAGCGGGGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCATACATGTGCAATGCCGTAACCTGGATAAAACGCGAAAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATcc                                                                                                                 <  4:215774/274‑1 (MQ=255)
                                                                                                                                                         gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGt                                                                                                                                                                                                                                                 <  8:651658/144‑1 (MQ=255)
                                                                                                                                                         gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGt                                                                                                                                                                                                                                                 >  7:651658/1‑144 (MQ=255)
                                                                                                                                                               gcgTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACAt                                                                                                         <  2:290252/274‑1 (MQ=255)
                                                                                                                                                                cgTGAACGCCTTATCCGCCATACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCCCATa                                                                                                        >  2:11038/1‑274 (MQ=255)
                                                                                                                                                                     ccGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAat                                                                                                   <  2:101606/273‑1 (MQ=255)
                                                                                                                                                                                 cccTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCTCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATca                                                                                                                                                                           >  2:554123/1‑190 (MQ=255)
                                                                                                                                                                                     aCATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACaaa                                                                                                                                                                       <  4:554122/190‑1 (MQ=255)
                                                                                                                                                                                              ttCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATg                                                                         >  2:17326/1‑275 (MQ=255)
                                                                                                                                                                                              ttCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCCACGGCTAAATTAGAACTCATCCGACCACATAACCATTCTTTTACCTACTGGACAATTTTATg                                                                         >  2:300569/1‑275 (MQ=255)
                                                                                                                                                                                                cccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCt                                                                                                                                                                                                                                                                           <  4:31650/79‑1 (MQ=255)
                                                                                                                                                                                                cccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATcc                                                                                                                                                                                                                 >  4:551573/1‑137 (MQ=255)
                                                                                                                                                                                                cccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGa                                                                                                                                      >  4:431061/1‑212 (MQ=255)
                                                                                                                                                                                                cccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCAt                                                                                                                   >  4:182121/1‑231 (MQ=255)
                                                                                                                                                                                                    tAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCg                                                                                                                                                                                                             <  2:551574/137‑1 (MQ=255)
                                                                                                                                                                                                    tAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGc                                                                                                                                  <  2:431062/212‑1 (MQ=255)
                                                                                                                                                                                                    tAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGa                                                                                                               <  2:182122/231‑1 (MQ=255)
                                                                                                                                                                                                                 aCGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCg                                                      >  1:35573/1‑275 (MQ=255)
                                                                                                                                                                                                                    cgACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaa                                                                                                                                                                                                                                     <  5:427192/97‑1 (MQ=255)
                                                                                                                                                                                                                    cgACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaa                                                                                                                                                                                                                                     >  6:427192/1‑97 (MQ=255)
                                                                                                                                                                                                                     gACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACcaca                                                                                                          >  4:23884/1‑219 (MQ=255)
                                                                                                                                                                                                                      aCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                                                                                                                                                       >  8:695299/1‑141 (MQ=255)
                                                                                                                                                                                                                      aCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                                                                                                                                                       <  7:695299/141‑1 (MQ=255)
                                                                                                                                                                                                                         aGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCggg                                                                                                                                                                                                  <  6:24839/127‑1 (MQ=255)
                                                                                                                                                                                                                         aGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAAc                                                                                                      <  2:23884/219‑1 (MQ=255)
                                                                                                                                                                                                                         aGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGGTATCACCggg                                                                                                                                                                                                  >  5:24839/1‑127 (MQ=255)
                                                                                                                                                                                                                           cGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGCCGGCTAAATTAGAACTCATCCGATCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTgg                                             >  2:139164/1‑274 (MQ=255)
                                                                                                                                                                                                                                  tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcg                                                                                                                                                                                                                                  <  8:1032522/86‑1 (MQ=255)
                                                                                                                                                                                                                                  tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcg                                                                                                                                                                                                                                  >  7:1032522/1‑86 (MQ=255)
                                                                                                                                                                                                                                  tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCg                                                                                                                                                                                                    <  8:164304/116‑1 (MQ=255)
                                                                                                                                                                                                                                  tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCg                                                                                                                                                                                                    <  8:358391/116‑1 (MQ=255)
                                                                                                                                                                                                                                  tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCg                                                                                                                                                                                                    >  7:358391/1‑116 (MQ=255)
                                                                                                                                                                                                                                  tCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCg                                                                                                                                                                                                    >  7:164304/1‑116 (MQ=255)
                                                                                                                                                                                                                                       cATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTAc                                <  2:230143/275‑1 (MQ=255)
                                                                                                                                                                                                                                          cTGCCCCCCGGCACCTGCGGATGCGGCGTCGACCCCTTACTCGCCCTACATGTGCAATTCCGTTACCTTGATACACNGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCa                              <  3:119017/274‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCAACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTaaaaaa                    >  2:102232/1‑274 (MQ=255)
                                                                                                                                                                                                                                                      aCTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACa                                                                                                                                                                                                                             >  8:944938/1‑71 (MQ=255)
                                                                                                                                                                                                                                                      aCTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACa                                                                                                                                                                                                                             <  7:944938/71‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAAACTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACcgcg               <  2:263966/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             gATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCAt                                                                                                                                                                             <  6:804655/112‑1 (MQ=255)
                                                                                                                                                                                                                                                             gATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCAt                                                                                                                                                                             >  5:804655/1‑112 (MQ=255)
                                                                                                                                                                                                                                                              ataaggcgTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGtt                                                                                                                                                                                                           <  6:1103661/77‑1 (MQ=18)
                                                                                                                                                                                                                                                              ataaggcgTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTc                                                                                                                                                             <  6:269000/123‑1 (MQ=37)
                                                                                                                                                                                                                                                               tggggcgTGTACTCCTTATCCGCCCTACNTNTGCAATTCCTTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGNTTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGNGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGAt        <  3:35573/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aggcgTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATc                                                                                                                                                                            >  6:300250/2‑109 (MQ=25)
                                                                                                                                                                                                                                                                 aggcgTCCACGCCGCATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATc                                                                                                                                                                            <  5:300250/108‑1 (MQ=37)
                                                                                                                                                                                                                                                                     gTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTc  <  2:92729/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                                            <  7:1114382/131‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTtctc                                                                                                                                            >  8:1114382/1‑131 (MQ=255)
                                                                                                                                                                                                                                                                            ccTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAg                                                                                                                          >  8:1062008/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                              ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                                                                                                                                                       <  6:865193/85‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCtttt                                                                                                                                                                                       >  5:865193/1‑85 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                         
GTATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAGAACTCATCCGACCACATAACAATTATTTTACATACTGGACAATTTTATGAGCTACCCGTCGCTGTTCGCCCCGCTGGATTTAGGTTTTACCACGTTAAAAAACCGCGTGTTGATGGGCTC  >  NC_000913/3231203‑3231738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: