Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,322,700 | A→G | 5.2% | intergenic (‑195/+33) | secG ← / ← glmM | preprotein translocase membrane subunit/phosphoglucosamine mutase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,322,700 | 0 | A | G | 5.2% | 386.5 / 8.0 | 115 | intergenic (‑195/+33) | secG/glmM | preprotein translocase membrane subunit/phosphoglucosamine mutase |
Reads supporting (aligned to +/- strand): ref base A (60/49); new base G (2/4); total (62/53) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.90e-01 |
Only 100 of 117 total aligned reads displayed.
AGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAC > NC_000913/3322441‑3322951 | agcaTGATCAGACAAACAAGGGCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACAGAATCAACCTGTTCCTTGAGGAGTTGCCGCGTACCAATGCTTCAACAAATACAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAGCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTa < 2:44913/275‑1 (MQ=255) agcaTGATCAGAACAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTa < 2:190970/275‑1 (MQ=255) caTGATCAAACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGc < 2:1482/275‑1 (MQ=255) caaGGCCAATTGCCACAATAAGGCAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAAAAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGACAGTTATCTAACGc > 1:56123/1‑275 (MQ=255) aaggaaaaTTGCAACAATAAGGAAACCTCNAAAAAGGGATTCATACATAATCACCNTGTTCCTGGCGGGGTTGCCGCGTACCAATGATTCAACCAATAAAGCGGTAGTTTTTAAGCTTTCCCACTGAAGCGGGTGTNAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCt < 3:93938/269‑1 (MQ=255) ggCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt > 2:73380/1‑275 (MQ=255) cacaATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCttt < 1:113884/275‑1 (MQ=255) ggaaaaaCTA‑TAAAAGCACTTCATACAAAACCAACCTGGTCCTTGCGGGGTTGCCGCGTACCAATGCTTCAACCAATAAGGCGGGAGTTTTTAATCTTTCCCCCGGAAGCGGGTGTGAATACCAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTAGCGCATTGTTTCACCTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCAt < 4:318735/272‑1 (MQ=255) actactAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCCAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTCACGCTTTAACCGGCTTTTACTGCAtcgtcg > 4:360122/1‑275 (MQ=255) actactAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCCTTTACTGCATCGGCg > 2:273929/1‑275 (MQ=255) aaaaGAGCTTCATACATAATCAACCTGTGCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGTAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCgg < 1:62450/275‑1 (MQ=255) gCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc > 1:36455/1‑274 (MQ=255) gCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc > 2:317692/1‑274 (MQ=255) gCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc > 2:357078/1‑274 (MQ=255) tCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAg > 4:21057/1‑219 (MQ=255) acataATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAg < 2:21057/219‑1 (MQ=255) aTCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTNTCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTCAGAAAAAAGGCGACAAACANCGCCTTTTTAGCCAGTTATCTAACGCt > 3:35590/1‑226 (MQ=255) aTCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCAcc > 1:148185/1‑275 (MQ=255) tCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCtttt > 4:542276/1‑206 (MQ=255) aCCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTaa < 1:35590/226‑1 (MQ=255) ccTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGc < 2:542277/206‑1 (MQ=255) ccTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAACCGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcg > 2:264175/1‑274 (MQ=255) acgTTCGTAGCGGTGTTGCCGCGTGCCAATGCTTCAACCAATAAAGGTGGAGTTGTTAAACTCTCCCACTGAAGCGTGTGGTAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCTCATTGTATCAACTGCGGAAAAAAACAGCAAACCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCt < 4:28488/273‑1 (MQ=255) tGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCtt > 2:12398/1‑274 (MQ=255) gCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCGTCGCTTCGTCTTc > 2:440427/1‑274 (MQ=255) aGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCtt > 2:342828/1‑275 (MQ=255) tGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGAAGTGTTCGCTGAGAAAAAAGGCGACAAACGCCGCCTTTTTAGCCAGTTATCTAACGCttt > 4:513155/1‑193 (MQ=255) tCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGGGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAAc < 2:513156/193‑1 (MQ=255) cGGGAGGTTTTAAACTTTCCCAATGAAGCGGGTGTGAATACAAACCAAACGGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATAAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGc < 4:9507/272‑1 (MQ=255) gTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCtt > 5:178386/1‑148 (MQ=255) tttttAATCTTGCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTAc < 2:399103/275‑1 (MQ=255) gcccACTGAAGCGGGTGTGAATACTAACCAAAGCAAATGACCTTCGCAAGGGAAATTTTATCGAATGGTATAAACGGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAacac < 1:111298/273‑1 (MQ=255) cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGc < 7:354666/139‑1 (MQ=255) cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGc > 8:354666/1‑139 (MQ=255) cTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa < 6:178386/148‑1 (MQ=255) aaGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAAAACGCGTCCAc > 1:130035/1‑274 (MQ=255) gtgtGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAGAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCAACCATCACGAGAATTAACGGTTGGGTGCCGGATTTATGCCGCAACACGAGTGCCAGGTTGc > 2:89607/1‑274 (MQ=255) tgtgAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCa < 5:745343/129‑1 (MQ=255) aataaacacaaaaGCGAATGAACTTTAGANNNCCAATTTTTGTGGATTACATCAACATGGAAAAAAAACAGGTAGACAGGTGTATTCGCTAAGAAAAAAAGCGACAATGGCCGCCTTTTTAGCCAGTTATCTAACGCTCTAAACGGCTTTTACTGCATCGGCGCTGCTGTGGGCAAATTCATTCACGTGCGCTTCGTCTTCGCCTTCCACCATCTCGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAgcg < 3:148185/265‑1 (MQ=255) aaaaaaaaCAAAAACGAAGGCCCGTCGANANGACAATTTTAGTGCATTGTATAAACTGCGGAAAAAATCAGCAAACNCGAGGGGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGNTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAgcg < 3:36455/269‑1 (MQ=255) aCTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttt > 8:731895/1‑115 (MQ=255) aCTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttt < 7:731895/115‑1 (MQ=255) tAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa > 8:333474/1‑129 (MQ=255) tAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa < 7:333474/129‑1 (MQ=255) tAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGACTTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCt > 4:428661/1‑275 (MQ=255) aaCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCCGCGCAGCt > 2:88017/1‑274 (MQ=255) aCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACATGCGCTTCGTCTTCGCCTTCCACCATCCAGCGAATTACAGGTTCGGTGCCGGATTTACGACCCAACACGCGTCCACGGTTGCCCAGCCCAGCTTc > 4:193432/1‑275 (MQ=255) cAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCtt < 8:391541/108‑1 (MQ=255) cAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCtt > 7:391541/1‑108 (MQ=255) cAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa > 7:978197/1‑124 (MQ=255) aaaGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTa < 7:830288/142‑1 (MQ=255) aaaGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTa > 8:830288/1‑142 (MQ=255) aaGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATc > 6:1048129/1‑148 (MQ=255) aTGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTg > 1:102673/1‑273 (MQ=255) tGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATg < 5:1048129/148‑1 (MQ=255) ttCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtg < 6:760693/148‑1 (MQ=255) ttCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtg > 5:760693/1‑148 (MQ=255) gaaaTTTTAGTGCAGTGTATCAACTCCGGAAAAAAACAGAAAAACCGATGTGTTCGCTGGGAAAAAAGGCGACAATCGCCGCCTTGTCAGCCAGTTATCTAACGCGTTAAACGGCTTGTACTGCATCGGCTATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCAGCACGCGAATTAACGGTTCGGTGCCTGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACt < 4:332833/273‑1 (MQ=255) gCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACCCGTCCACGGTTTCCCAGCGCAGCTTCAACCCCTTCGGTCCCGGCTTTaa > 1:127606/1‑273 (MQ=255) cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCttttt < 7:755673/87‑1 (MQ=255) cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCttttt > 8:755673/1‑87 (MQ=255) cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCg < 8:730239/135‑1 (MQ=255) cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCg > 7:730239/1‑135 (MQ=255) aTTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTAt > 6:131048/1‑95 (MQ=255) aTTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTAt < 5:131048/95‑1 (MQ=255) ttttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACt < 4:317691/275‑1 (MQ=255) ttATCGAATTGAATCAACTGCGAAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGACTTTTTAGACAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCa < 4:357077/275‑1 (MQ=255) cGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCtt > 3:21819/1‑97 (MQ=255) ttGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTaaa < 1:21819/97‑1 (MQ=255) tGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGCCTCATGCCCAAg > 4:481949/1‑274 (MQ=255) cAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTc < 7:455201/128‑1 (MQ=255) cAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTc > 8:455201/1‑128 (MQ=255) aCTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa < 5:1091624/79‑1 (MQ=255) aCTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa > 6:1091624/1‑79 (MQ=255) cTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATcgcc > 4:308247/1‑275 (MQ=255) tgatgAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTGTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCATCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATcgccg < 4:243611/271‑1 (MQ=255) gCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCCCAAGGTGATcccccc > 4:69516/1‑273 (MQ=255) ggAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACCCGCCCACGGTTGCCCACCGCAGCTTCAACCTCTCCGGTCACGGCTTTAATTGACTCATCCTCAAGTGGATccccg > 2:516185/1‑272 (MQ=255) ggAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCCATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCGTCGCCTTCCACCCTCACGCTAATTAACGGTTCGGTACCGGATTTCCGCAGCAACCCGCCTCCAAGGTTGCCCAGTGAATCTTCCACCCCTGCCGTCACGGCTTTAAACGACTCATGACCAAGTGGacgcccacc > 4:62437/1‑266 (MQ=255) gAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGCTTTACGCAGCAACACGCNCCCACGGTTGCCCAGCGCAGCTTAAACTCCTCCGGCCACGGCCGTAACTGACTCATGCTCAAGCGGTACGCCGCtcc > 3:115989/1‑273 (MQ=255) aaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCgg < 5:203696/106‑1 (MQ=255) aaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCgg > 6:203696/1‑106 (MQ=255) aCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTc < 7:589327/113‑1 (MQ=255) aCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTc > 8:589327/1‑113 (MQ=255) aCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGgt < 4:264174/275‑1 (MQ=255) aaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTc < 5:422683/134‑1 (MQ=255) aaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTc > 6:422683/1‑134 (MQ=255) aTGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt > 5:379674/1‑72 (MQ=255) aTGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt < 6:379674/72‑1 (MQ=255) tgtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCtt > 8:910741/1‑57 (MQ=255) tCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCa > 6:679394/1‑96 (MQ=255) tCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCa < 5:679394/96‑1 (MQ=255) cGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAc < 1:115989/271‑1 (MQ=255) gagaAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTaaa < 6:87098/53‑1 (MQ=255) aaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTa < 6:518609/148‑1 (MQ=255) aaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTTACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAt < 5:964371/144‑1 (MQ=255) aCAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtg < 6:428048/68‑1 (MQ=255) aCAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtg > 5:428048/1‑68 (MQ=255) cAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCtt < 6:376033/96‑1 (MQ=255) aaTCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc > 7:656950/1‑69 (MQ=255) | AGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAC > NC_000913/3322441‑3322951 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |