Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,354,537 C→G 15.2% intergenic (‑487/‑188) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,354,5370CG15.2% 313.5 / 46.5 105intergenic (‑487/‑188)yhcC/gltBputative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base C (48/41);  new base G (9/7);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

Only 100 of 107 total aligned reads displayed.

TATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCC  >  NC_000913/3354289‑3354778
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
tataAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAAtt                                                                                                                                                                                                                         >  2:510865/1‑275 (MQ=255)
 ataAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAAtt                                                                                                                                                                                                                         >  2:218374/1‑274 (MQ=255)
                                                   ccTTTATGAAAGTTAGGAATTGACTGTTTCTCTAACCACTGCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggggg                                                                                                                                                                       <  1:132667/274‑1 (MQ=255)
                                                                             tttCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCata                                                                                                                                                                                                                                       >  4:555169/1‑184 (MQ=255)
                                                                                 tctAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTa                                                                                                                                                                                                                                   <  2:555170/184‑1 (MQ=255)
                                                                                         cTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                                                                                                <  4:218373/275‑1 (MQ=255)
                                                                                               ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGct                                                                                                                          <  4:510864/275‑1 (MQ=255)
                                                                                               ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGct                                                                                                                          <  2:182205/275‑1 (MQ=255)
                                                                                                                    ccATTTTAATTTCATTCATTTAATAAAGAATTTTGCGCTAAAGCACCTTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                                                                                                      <  4:47039/274‑1 (MQ=255)
                                                                                                                                           taaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                                                                                                                                                                              <  8:728998/115‑1 (MQ=255)
                                                                                                                                           taaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                                                                                                                                                                              >  7:728998/1‑115 (MQ=255)
                                                                                                                                                    ttGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                                                                                                                                                                              <  8:913279/106‑1 (MQ=255)
                                                                                                                                                    ttGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                                                                                                                                                                              >  7:913279/1‑106 (MQ=255)
                                                                                                                                                         cTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatc                                                                                                                                                                                                                                          >  5:1026853/1‑105 (MQ=255)
                                                                                                                                                           aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                                                                                                                                                                                 <  5:322890/112‑1 (MQ=255)
                                                                                                                                                           aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                                                                                                                                                                                 >  6:322890/1‑112 (MQ=255)
                                                                                                                                                           aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTg                                                                                                                                                                                                                        >  7:801867/1‑121 (MQ=255)
                                                                                                                                                           aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTg                                                                                                                                                                                                                        <  8:801867/121‑1 (MQ=255)
                                                                                                                                                                   tttCTGTACCAATAAGCTTGCCATTTGACCTGTATCGGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcat                                                                                                                                                                                                                                           <  7:923600/94‑1 (MQ=255)
                                                                                                                                                                         tACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGCCAACAAGGGGGCGAATGCGAGGCCCGCGTCTGCCACCACAACCCCGTCGCCACGCCCTTTCGTTGCCCGTGCGCACCGGTTCGGAAGTGGGGTTCCCGCCGGGCCTGGGGGATGTTCACGATCtgttgt                                                 >  4:73291/1‑274 (MQ=255)
                                                                                                                                                                          aCCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTg                                                                                                                                                                                                                        >  8:444438/1‑106 (MQ=255)
                                                                                                                                                                          aCCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGacaac                                                                                                                                                                              >  7:1048006/1‑148 (MQ=255)
                                                                                                                                                                            cAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatc                                                                                                                                                                                                                                          >  5:173353/1‑86 (MQ=255)
                                                                                                                                                                            cAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatc                                                                                                                                                                                                                                          <  6:173353/86‑1 (MQ=255)
                                                                                                                                                                              aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCTGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAGGACACGCAAACCACGTCGCCACGCCCTTTCTGTCCCCGTGCGCAGCGGTTCGGACGGGGGGTTCCCGCAGGGCCTGGGGGGGGTTCACGATGTGTTGTACGATa                                           >  4:11708/1‑275 (MQ=255)
                                                                                                                                                                              aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATc                                                                                                                                                                                                                   >  5:220663/1‑107 (MQ=255)
                                                                                                                                                                              aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATc                                                                                                                                                                                                                   <  6:220663/107‑1 (MQ=255)
                                                                                                                                                                              aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGCTATGTTGTACGATa                                           >  1:4293/1‑275 (MQ=255)
                                                                                                                                                                               tAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                                                                                                                                                                                >  7:1073295/1‑93 (MQ=255)
                                                                                                                                                                               tAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                                                                                                                                                                                <  8:1073295/93‑1 (MQ=255)
                                                                                                                                                                               tAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggg                                                                                                                                                                         >  8:683711/1‑148 (MQ=255)
                                                                                                                                                                               tAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                                                                                                                           <  6:864646/146‑1 (MQ=255)
                                                                                                                                                                               tAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                                                                                                                           >  5:864646/1‑146 (MQ=255)
                                                                                                                                                                                     tGCCATTTGACCTGTCTCAGCTTTCCCGATGCGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGCAACGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTTCGAAGCGGTTCGGAAGTGCGGTTCACGCATCGCCTGGGGGAGGTTCCAGCTCTGTTGGACGATAAATCCCt                                    >  4:162762/1‑275 (MQ=255)
                                                                                                                                                                                      gCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAACTCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGCTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGCCACGCCAACCCCGTCGCCCCGCCCTTTCTGTGCCCGTGCGCAGAGGTACGGCAGTGGGGTTCCCGCACCCGCGGGCGGNNNGTCACGGTATGATGTACGATaaacgcctc                                   >  3:145931/1‑269 (MQ=255)
                                                                                                                                                                                      gCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGt                                                                                                                                                                                                      <  6:193165/112‑1 (MQ=255)
                                                                                                                                                                                      gCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGGGCTGGGGGGAGGTTCACGATATGTTGTACGATAAATc                                       >  1:88425/1‑271 (MQ=255)
                                                                                                                                                                                      gCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCGCTTTCTGTGCCCGTGCGCAACGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATCAGTTGTACGATAAATc                                       >  2:213717/1‑271 (MQ=255)
                                                                                                                                                                                       ccATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGa                                                                                                                                                                                             >  8:631199/1‑120 (MQ=255)
                                                                                                                                                                                       ccATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGa                                                                                                                                                                                             <  7:631199/120‑1 (MQ=255)
                                                                                                                                                                                          tttGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCg                                                                                                                                                                                         <  5:553406/121‑1 (MQ=255)
                                                                                                                                                                                          tttGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCg                                                                                                                                                                                         >  6:553406/1‑121 (MQ=255)
                                                                                                                                                                                               ccTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa                                                                                                                                                                                  >  7:422167/1‑123 (MQ=255)
                                                                                                                                                                                               ccTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa                                                                                                                                                                                  <  8:422167/123‑1 (MQ=255)
                                                                                                                                                                                                 tGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc                                                                                                                                                       >  5:478161/1‑148 (MQ=255)
                                                                                                                                                                                                  gTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGCTGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATACCCTCTTCACCGTATTAACCGACGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCTCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTATGTGCCCGTGCGCCGCGGTTCGGAAGTGGGGTCACCGCAGCGCCTGGTGGACGTTCCCGATCTGTTGTACGATAAATCCATTGAAGGGGTTAAc                       >  4:236139/1‑275 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcatc                                                                                                                                                                                                                                          <  8:239689/60‑1 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcatc                                                                                                                                                                                                                                          >  7:239689/1‑60 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTg                                                                                                                                                                                                                        <  5:691435/78‑1 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                                                                                                                           <  8:197005/123‑1 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                                                                                                                           >  7:197005/1‑123 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa                                                                                                                                                              >  5:120254/1‑136 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa                                                                                                                                                              <  6:120254/136‑1 (MQ=255)
                                                                                                                                                                                                      cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                                                                  >  5:646769/1‑148 (MQ=255)
                                                                                                                                                                                                           ttCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACg                                                                                                                                             <  6:478161/148‑1 (MQ=255)
                                                                                                                                                                                                             cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtc                                                                                                                                                                                                                                             >  1:110797/1‑50 (MQ=255)
                                                                                                                                                                                                               cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                                                                                                               >  6:412349/1‑126 (MQ=255)
                                                                                                                                                                                                               cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                                                                                                               <  5:412349/126‑1 (MQ=255)
                                                                                                                                                                                                               cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa                                                                                                                                         <  6:646769/148‑1 (MQ=255)
                                                                                                                                                                                                                 aTAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                                                                                                                                                                                <  1:136350/43‑1 (MQ=255)
                                                                                                                                                                                                                 aTAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcatca                                                                                                                                                                                                                                         <  3:110797/50‑1 (MQ=255)
                                                                                                                                                                                                                 aTAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGAcaacaa                                                                                                                                                                            <  7:818741/111‑1 (MQ=255)
                                                                                                                                                                                                                 aTAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGAcaacaa                                                                                                                                                                            >  8:818741/1‑111 (MQ=255)
                                                                                                                                                                                                                    aGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCg                                                                                                                                    <  8:1048006/148‑1 (MQ=255)
                                                                                                                                                                                                                        ggAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCCCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTGCCCGCAGAGCGTGGGGGAGGTTAACGATATGTCGTACGATAAATCCCTTGAGAGGGATAACGGTGGTTTCGGCCTGATCGcc  >  1:117400/1‑274 (MQ=255)
                                                                                                                                                                                                                            aTCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                                                                                                                                                                                <  5:20304/48‑1 (MQ=255)
                                                                                                                                                                                                                            aTCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                                                                                                                                                                                >  6:20304/1‑48 (MQ=255)
                                                                                                                                                                                                                                 cTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                                                                                                               <  6:1010071/108‑1 (MQ=255)
                                                                                                                                                                                                                                 cTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                                                                                                               >  5:1010071/1‑108 (MQ=255)
                                                                                                                                                                                                                                  tGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggg                                                                                                                                                                         >  6:1053777/1‑97 (MQ=255)
                                                                                                                                                                                                                                  tGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggg                                                                                                                                                                         <  5:1053777/97‑1 (MQ=255)
                                                                                                                                                                                                                                    gAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggg                                                                                                                                                                         <  8:774003/95‑1 (MQ=255)
                                                                                                                                                                                                                                    gAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggg                                                                                                                                                                         >  7:774003/1‑95 (MQ=255)
                                                                                                                                                                                                                                     aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                                                                                                                   >  5:313894/1‑84 (MQ=255)
                                                                                                                                                                                                                                     aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                                                                                                                   <  6:313894/84‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa                                                                                                                                                              >  8:445216/1‑105 (MQ=255)
                                                                                                                                                                                                                                     aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa                                                                                                                                                              <  7:445216/105‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                                                                                                                        <  7:379380/111‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                                                                                                                        >  8:379380/1‑111 (MQ=255)
                                                                                                                                                                                                                                      aGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                                                                                                               >  5:184432/1‑103 (MQ=255)
                                                                                                                                                                                                                                      aGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                                                                                                               <  6:184432/103‑1 (MQ=255)
                                                                                                                                                                                                                                            tatgATGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATg                                                                                                                                                                 >  8:1020486/4‑95 (MQ=255)
                                                                                                                                                                                                                                            tatgATGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATg                                                                                                                                                                 <  7:1020486/92‑1 (MQ=255)
                                                                                                                                                                                                                                               gATGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                                                                                                                   >  6:939406/1‑74 (MQ=255)
                                                                                                                                                                                                                                               gATGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                                                                                                                   <  5:939406/74‑1 (MQ=255)
                                                                                                                                                                                                                                               gATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgc                                                                                                                                                           <  5:926715/98‑1 (MQ=255)
                                                                                                                                                                                                                                               gATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgc                                                                                                                                                           >  6:926715/1‑98 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTaa                                                                                                                                                                                                 >  7:641783/1‑59 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTaa                                                                                                                                                                                                 <  8:641783/59‑1 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc                                                                                                        >  5:11844/1‑148 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc                                                                                                        >  6:592851/1‑148 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAg                                                                                                                                                             <  7:217820/95‑1 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                                                                                                   >  6:868815/1‑137 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg                                                                                                                   <  5:868815/137‑1 (MQ=255)
                                                                                                                                                                                                                                                aTGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc                                                                                                        >  8:27782/1‑148 (MQ=255)
                                                                                                                                                                                                                                                 tGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggggg                                                                                                                                                                       <  5:1091363/84‑1 (MQ=255)
                                                                                                                                                                                                                                                 tGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggggg                                                                                                                                                                       >  6:1091363/1‑84 (MQ=255)
                                                                                                                                                                                                                                                  gAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg                                                                                                      <  7:27782/148‑1 (MQ=255)
                                                                                                                                                                                                                                                     cagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                                                                                                      >  8:159944/1‑113 (MQ=255)
                                                                                                                                                                                                                                                     cagcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc                                                                                                                                      <  7:159944/113‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
TATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCC  >  NC_000913/3354289‑3354778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: