Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,621 C→G 6.2% noncoding (35/76 nt) thrV ← tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,6210CG 6.2% 675.2 / 26.9 193noncoding (35/76 nt)thrVtRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (92/88);  new base G (7/5);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

Only 100 of 194 total aligned reads displayed.

CGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCA  >  NC_000913/3423348‑3423893
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                
cGGGAGGGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTAc                                                                                                                                                                                                                                                                                  <  2:538484/274‑1 (MQ=255)
  ggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCa                                                                                                                                                                                                                                                                                <  2:345908/274‑1 (MQ=255)
                 acaaacaaCAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGACTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCa                                                                                                                                                                                                                                                                >  4:451729/1‑275 (MQ=255)
                       aacaGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                                                                                                                                                                                                                                          <  4:142274/275‑1 (MQ=255)
                                 aCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGTCGCTACGGCGTTTCACTTCTGAGTTAGGCATGGGGTCAGGTCAGACCAGCGCGCCACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTCATCTTAAAAGTCGTGCTGATACACAGTGTCGCACTGGGGAACTACCCCTTACCAAGGGTGGCCCCTCCCAACCACGCCatattcac                                                                                                                                                                                                                                                >  4:33032/1‑271 (MQ=18)
                                 aCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                                                                                                                                                                                                                                              >  4:125905/1‑261 (MQ=255)
                                 aCAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCCCACCCTTACCAAGGGTCCGCCCTACCAACTGAGCCATATCAGc                                                                                                                                                                                                                                                >  4:507444/1‑275 (MQ=255)
                                     aaGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                                                                                                                                                                                                                                          <  2:125906/261‑1 (MQ=255)
                                                   cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTTCCGCCAGACAACTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTTATGcc                                                                                                                                                                                                                               >  2:271001/1‑274 (MQ=255)
                                                       tGAGCCTTTTGTTTTATTTGATGTCGGGCAGTCCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg                                                                                                                                                                                                                            <  2:507445/273‑1 (MQ=255)
                                                       tGAGACTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTgg                                                                                                                                                                                                                            <  2:451730/273‑1 (MQ=255)
                                                                 gTTTTATTTGTGGTCTGGGAGTTCCCTACTCTCGCGTCAGGAGCCCCCACACTACCCTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATTGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCTAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACCGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAct                                                                                                                                                                                                                <  4:152893/275‑1 (MQ=37)
                                                                      aTTTGATTGCTGGCAGTTCCCTACTCTCGCATGTGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCGCCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                                                                                                           <  2:63379/275‑1 (MQ=255)
                                                                             gTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACATTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGga                                                                                                                                                                                                    <  2:33033/275‑1 (MQ=255)
                                                                                                            aCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGAt                                                                                                                                                                                                                                  >  2:379061/1‑214 (MQ=255)
                                                                                                             ccccACACGACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTTCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCGCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                                                                                                    <  1:145478/275‑1 (MQ=255)
                                                                                                                  cacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                                                                                                                                                            >  3:136186/1‑166 (MQ=255)
                                                                                                                                         ttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcgc                                                                                                                                                                                                                                                                       >  6:38875/1‑148 (MQ=255)
                                                                                                                                             cTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                                                                                                                                     >  2:163369/1‑274 (MQ=255)
                                                                                                                                               tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                                                                                                                                                            >  6:49557/1‑137 (MQ=255)
                                                                                                                                               tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                                                                                                                                                  <  7:624198/147‑1 (MQ=255)
                                                                                                                                                 tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgc                                                                                                                                                                                                                                                                         <  7:182937/138‑1 (MQ=255)
                                                                                                                                                 tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgc                                                                                                                                                                                                                                                                         >  8:182937/1‑138 (MQ=255)
                                                                                                                                                    gTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                                                                                                                                                            <  8:46636/148‑1 (MQ=255)
                                                                                                                                                      tCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                                                                                                                                                                                                                                          >  7:532479/1‑148 (MQ=255)
                                                                                                                                                             ggggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATc                                                                                                                                                                                                                                                   <  5:38875/148‑1 (MQ=255)
                                                                                                                                                              gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTTCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCa                                                                                                                                                                                                                                                  <  8:532479/148‑1 (MQ=255)
                                                                                                                                                                    ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGctc                                                                                                                                                                                                                                                                     <  6:93661/123‑1 (MQ=255)
                                                                                                                                                                    ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGc                                                                                                                                                                                                                                            >  7:352975/1‑148 (MQ=255)
                                                                                                                                                                      tGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                                                                                                                                                            <  7:583191/130‑1 (MQ=255)
                                                                                                                                                                      tGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                                                                                                                                                            >  8:583191/1‑130 (MQ=255)
                                                                                                                                                                        ggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAct                                                                                                                                                                                                                <  1:10670/172‑1 (MQ=255)
                                                                                                                                                                         gACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAt                                                                                                                                                                                                                                       <  8:352975/148‑1 (MQ=255)
                                                                                                                                                                          accaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAtt                                                                                                                                                                                                                                      >  5:929084/1‑148 (MQ=255)
                                                                                                                                                                           ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                                                                                                                                                                                                                                                    >  5:1038937/1‑133 (MQ=255)
                                                                                                                                                                           ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                                                                                                                                                                                                                                                    <  6:1038937/133‑1 (MQ=255)
                                                                                                                                                                               cgcgCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCata                                                                                                                                                                                                                                                     >  2:1497/1‑128 (MQ=255)
                                                                                                                                                                               cgcgCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATCTCAGCACGCTAAATTTGATCCCTGGCAGTTCCCTACTCTCGCATGGGCAGACCCCACCCTACCCACGGCCCTCCGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCACGCTCCGGCCGCCAGGAAAATTCTGt                                                                                                    >  4:65156/1‑273 (MQ=39)
                                                                                                                                                                                   cTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTg                                                                                                                                                                                                                             <  7:756274/148‑1 (MQ=255)
                                                                                                                                                                                            ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTga                                                                                                                                                                                                                                                                         >  8:318362/1‑94 (MQ=255)
                                                                                                                                                                                                    aTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                                                                                                                                                                                                                                   >  8:618162/1‑125 (MQ=255)
                                                                                                                                                                                                      tCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                                                                                                                                                    <  7:412437/122‑1 (MQ=255)
                                                                                                                                                                                                      tCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                                                                                                                                                    >  8:412437/1‑122 (MQ=255)
                                                                                                                                                                                                         tttCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTTCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTATCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTGCGGTATGGGGTACGGTGGGACCACAGCGCAAAGGCCGCCAGGAAAATGCTGTTTTATCGAACCGCATCTGCGTTCTGAtt                                                                        >  1:111971/1‑275 (MQ=25)
                                                                                                                                                                                                              aaTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGct                                                                                                                                                                                                                                                                      >  8:56427/1‑80 (MQ=255)
                                                                                                                                                                                                              aaTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGct                                                                                                                                                                                                                                                                      <  7:56427/80‑1 (MQ=255)
                                                                                                                                                                                                                tCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAg                                                                                                                                                                                                                                                                              <  6:620318/70‑1 (MQ=255)
                                                                                                                                                                                                                tCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAg                                                                                                                                                                                                                                                                              >  5:620318/1‑70 (MQ=255)
                                                                                                                                                                                                                     cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCata                                                                                                                                                                                                                                                     >  8:908616/1‑90 (MQ=255)
                                                                                                                                                                                                                     cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaaa                                                                                                                                                                                                                                        <  8:850069/103‑1 (MQ=255)
                                                                                                                                                                                                                     cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg                                                                                                                                                                                                                         <  5:140422/118‑1 (MQ=255)
                                                                                                                                                                                                                     cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCt                                                                                                                                                                                                                   <  7:862378/124‑1 (MQ=255)
                                                                                                                                                                                                                           ttAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGt                                                                                                                                                                                                                                                                           <  7:924514/62‑1 (MQ=255)
                                                                                                                                                                                                                                 tAAAAAGTGGTGCTGATACCCAGCGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                                                                                                                                                                >  8:1073196/1‑131 (MQ=255)
                                                                                                                                                                                                                                 tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTcc                                                                                                                                                                                                                     >  7:846420/1‑110 (MQ=255)
                                                                                                                                                                                                                                 tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTActct                                                                                                                                                                                                              <  8:596278/117‑1 (MQ=255)
                                                                                                                                                                                                                                 tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                                                                                                                                                  <  5:491244/129‑1 (MQ=255)
                                                                                                                                                                                                                                 tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                                                                                                                                                                <  7:1073196/131‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                                                                                                                                                            <  8:52082/54‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                                                                                                                                                            >  7:52082/1‑54 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGc                                                                                                                                                                                                                                                         >  6:412892/1‑73 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGc                                                                                                                                                                                                                                                         <  5:412892/73‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                                                                                                           >  5:889084/1‑119 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt                                                                                                                                                                                         >  6:1129041/1‑137 (MQ=255)
                                                                                                                                                                                                                                  aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcgc                                                                                                                                                                              >  6:209100/1‑148 (MQ=255)
                                                                                                                                                                                                                                   aaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                                                                                                                                                            >  2:539669/1‑53 (MQ=255)
                                                                                                                                                                                                                                   aaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAAtcttc                                              >  2:298057/1‑275 (MQ=255)
                                                                                                                                                                                                                                           tgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCaa                                                                                                                                                                                                                                                               >  7:499092/1‑58 (MQ=255)
                                                                                                                                                                                                                                             cTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAACACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCg                                     >  2:94400/1‑274 (MQ=255)
                                                                                                                                                                                                                                               gATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGc                                                                                                                                                                                                                                >  5:853856/1‑85 (MQ=255)
                                                                                                                                                                                                                                                aTACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                                                                                                                                                                >  7:455249/1‑116 (MQ=255)
                                                                                                                                                                                                                                                aTACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                                                                                                                                                                <  8:455249/116‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                                                                                                                                                              >  6:926115/1‑146 (MQ=255)
                                                                                                                                                                                                                                                     cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaa                                                                                                                                                                                                                                         >  5:349055/1‑70 (MQ=255)
                                                                                                                                                                                                                                                     cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaa                                                                                                                                                                                                                                         <  6:349055/70‑1 (MQ=255)
                                                                                                                                                                                                                                                     cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                                                                                                                                                              >  8:1131807/1‑113 (MQ=255)
                                                                                                                                                                                                                                                     cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                                                                                                                                                              <  7:1131807/113‑1 (MQ=255)
                                                                                                                                                                                                                                                     cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                                                                                                           <  8:745206/116‑1 (MQ=255)
                                                                                                                                                                                                                                                      agagTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                                                                                                                                      <  7:30349/136‑1 (MQ=255)
                                                                                                                                                                                                                                                      agagTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                                                                                                                                      >  8:30349/1‑136 (MQ=255)
                                                                                                                                                                                                                                                             aaCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                                                                                                                         >  6:858748/1‑142 (MQ=255)
                                                                                                                                                                                                                                                               cTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATg                                                                                                                                                 <  5:1120355/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac                                                                                                                                                                                            >  6:258402/1‑99 (MQ=255)
                                                                                                                                                                                                                                                                     aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac                                                                                                                                                                                            <  5:258402/99‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                                                                                                                                                              >  5:720471/1‑129 (MQ=255)
                                                                                                                                                                                                                                                                        tCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCa                                                                                                                                                                                     >  7:727501/1‑103 (MQ=255)
                                                                                                                                                                                                                                                                        tCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                                                                                                                        >  6:716758/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                        tCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                                                                                                                        >  7:1024140/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                        gcACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGCCAGGTGGGACCACCGCGCTACGGCAGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCTTCGGCGTTGTAAGGtt          >  4:291003/2‑274 (MQ=255)
                                                                                                                                                                                                                                                                         cACCCTTGGTAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc                                                                                                                                                                                                                    >  6:627424/1‑71 (MQ=25)
                                                                                                                                                                                                                                                                         cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                                                                                                          <  6:812190/145‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                                                                                                                                                                 >  8:37871/1‑104 (MQ=255)
                                                                                                                                                                                                                                                                           cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                                                                                                                                                                 <  7:37871/104‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                                                                                                                                     <  8:1024140/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGa                                                                                                                                    >  6:1075702/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                             cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATg                                                                                                                                                                                                                                 <  8:657529/54‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                                                                                                           <  8:773184/92‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                                                                                                                                                            >  5:93662/1‑123 (MQ=255)
                                                                                                                                                                                                                                                                             cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                                                                                                                                                        >  7:611280/1‑127 (MQ=255)
                                                                                                                                                                                                                                                                                aCCAAGGATACGAGCTACCAACTGAGGCATATCAGAACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCa  <  2:7983/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                
CGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCA  >  NC_000913/3423348‑3423893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: