| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,458,418 | T→A | 6.3% | I27N (ATT→AAT) | gspE → | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,458,418 | 0 | T | A | 6.3% | 395.7 / 13.3 | 112 | I27N (ATT→AAT) | gspE | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base T (49/56); new base A (3/4); total (52/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.49e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATT > NC_000913/3458146‑3458683 | tccgACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACTCCCCTGCGCCATCGTCACGGTTAGGCTTTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGattatt > 2:187784/1‑274 (MQ=255) cgacgaTTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGACA‑ACACGTTCAACCGTCACGCCCCTGCGCCATCGTCACTGTGAGTCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCCCAACGCATTGGTTATCTCTTTTCAGAGGGCGAGCttatttat > 4:378968/1‑275 (MQ=255) tgatgTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGCCGAGATTATTTATCTCCCCGATACGCCATTcc > 4:472294/1‑274 (MQ=255) aCTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGt > 2:36345/1‑266 (MQ=255) tGTCAAAAGGGAAATAAACCCGTTACCGTCAGGGGCAAAAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATAtt < 4:66789/275‑1 (MQ=255) tCAAAAGAGAAAAAAACCCGTTACCGGCAGGAGCAACACCAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACt < 4:36344/266‑1 (MQ=255) gaAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAgg > 2:199463/1‑275 (MQ=255) cacCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGcc > 2:160300/1‑275 (MQ=255) gTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCTAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTg > 4:333511/1‑274 (MQ=255) cGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGAc < 2:455023/275‑1 (MQ=255) cAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTCTTCAGACGGNGAGATTATTTATCTCGCCGATACCCCCTTCGACCGGGTCCTCGAATTTCAACCGCAGGTTGGCCCGTGCCaccaca > 3:131804/1‑269 (MQ=255) gAGCAACAACAGCGGATCGACGGGAAATCTAAAGCGCTGGTTGGCGTGGAAGATTGTCGGGTGCTGGATGAAA‑AAACATTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCAATCACCGTACCCCGCCAGTTGGGCGCTGGGACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGAc < 2:378969/275‑1 (MQ=255) gAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGAc < 2:251151/275‑1 (MQ=255) acaccaGCGGATCGACGGGAAATAAAAAGCGCTGGGTGGGTCGGAAGATTTGCCGGTTCTGGATGAAA‑ACCGGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGAACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCt < 4:435073/275‑1 (MQ=255) aGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCCGGTTGGCCCGTGCCAGACCATTACCACCTTGTca > 2:350229/1‑275 (MQ=255) ccaaaGCGTCGGTTTGCCCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTAAACAGTCCCGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCGATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCt < 4:199462/273‑1 (MQ=255) gcTTGTTGTCTTGGAATATAGGCCGGTGCTGGGTTAAAGACA‑GTGCAACAGTCATGCCCCTGCGCCGTCGTTACGGTGAGGCGTTGCTATGAGAATTCCATCACCGTACCCCGCCAGTGGTGCGCTGGCACTACGAATTGCTTATCTATATTCAGAGGGCGAGATTATTTATCTGGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTgg < 4:287037/275‑1 (MQ=255) gTTGGCTCTGACTTTTTTCTGGTTCTGGANCAAA‑CCACTTTGTAGAGTCCCGCCCNTCCGGCAGCGTCACGGTGAGGCTTTCATATTAGAATTCCCTCACCGTACCCCTCCAGTTGGGCGCTGGCACAACGAAGTGNTTATCTCTATTCAGTGGGCGTGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTAGTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc < 3:86026/275‑1 (MQ=255) cTCGGAAGATTTGCCGGGGCTGGGTGAAA‑ACACGTTAAACAGTCCCGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTAGTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAt < 4:288827/275‑1 (MQ=255) cGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCAAAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTc < 2:162018/275‑1 (MQ=255) aGATTTGCCGGTGCTGGAGGAAA‑AAACGTTCAACAGGCACGCCCGTGCGCCATCGTCACGGTGAGGCATTAATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTAATCAGAGGGCGGGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAt < 2:348399/274‑1 (MQ=255) tggcGGTGCTGAATGAAA‑ACACGTTCACCAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGaa < 4:508159/271‑1 (MQ=255) tGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGt > 2:420093/1‑275 (MQ=255) tGGATGAAA‑ACACGTTAAACCGGCCCGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAg < 1:121945/275‑1 (MQ=255) aa‑aCACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCCGCTTTTCACAGGCTGAATTTGACGCTCGGCTGGAAGCGGTATTCCATCAGAATTCCGGTTATTCACAAc > 4:315004/1‑275 (MQ=255) aa‑aCACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAAc > 2:260200/1‑275 (MQ=255) aa‑aCACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAAc > 2:179993/1‑275 (MQ=255) a‑aCACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAAc < 2:104995/274‑1 (MQ=255) ttCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGAttattta < 6:768664/128‑1 (MQ=255) ttCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGAttattta > 5:768664/1‑128 (MQ=255) cccacgtccccccccTGCGCAATCGGCCCGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCATTTGGGCGCTGGACAAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTgcg < 4:433593/263‑1 (MQ=255) cAACAGTCACGCCCCTGCGCCATCGTAACGGTGAGGCATTAATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTgcg < 2:388180/274‑1 (MQ=255) aCAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGa > 8:387257/1‑145 (MQ=255) aTCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGAttattt < 8:418089/104‑1 (MQ=255) aTCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGAttattt > 7:418089/1‑104 (MQ=255) aTCGTCACGGTGAGGCATTCAAATGAGAATTCACTCACCGGACCCCGCCAGTTAGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTAGCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGt < 2:139600/273‑1 (MQ=255) cGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGattatt > 6:195076/1‑101 (MQ=255) cGTCACGATGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGattatt < 5:195076/101‑1 (MQ=255) tatGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGAcc > 5:431221/1‑148 (MQ=255) aaTTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAg > 7:237384/1‑100 (MQ=255) aaTTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAg < 8:237384/100‑1 (MQ=255) aaTTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGat < 5:495560/112‑1 (MQ=255) aaTTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGAcc > 7:1082531/1‑148 (MQ=255) aaTTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTAGAGCGGTTACTCGat > 6:495560/1‑112 (MQ=255) aTTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTgg > 3:137029/1‑181 (MQ=255) ttCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGt > 7:443854/1‑119 (MQ=255) tcactcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATAAATAATCTCGc < 7:252642/81‑1 (MQ=255) actcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt < 8:1082531/148‑1 (MQ=255) actcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc < 1:137029/181‑1 (MQ=255) actcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCCCCCGCTTTCGGAAGAGATGccc > 4:443679/1‑272 (MQ=255) actcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCCCGGCTGGAATCGGTATTCCATTAGAATACCGGTGATTAGCAACAGATTGCGCAGGATCTCTCACAATCAGTCGATCTTCTCCCGCTTTCGGAAgagaacgcc > 4:355588/1‑267 (MQ=255) ctcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCa > 8:225311/1‑88 (MQ=255) tcacCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGt > 5:743507/1‑148 (MQ=255) ccGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTc > 7:323153/1‑112 (MQ=255) ccGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTc < 8:323153/112‑1 (MQ=255) cGCCAGTTGGTCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGt > 7:668429/1‑116 (MQ=255) cGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGt < 8:668429/116‑1 (MQ=255) gCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt > 6:771728/1‑148 (MQ=255) gCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCACCAGAATACCGGTGAGTCGCAACAGATTGCCCAGGACATCGAACAATCCGGCGATCTTCTCGCCCTTTCGGAATCGATGCCCGCAAAagaagaccacc > 4:260050/1‑269 (MQ=255) ggtGGGCGCTGGAACAACGAATTGGTTATTTCTATTCAGAGGGCGGGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCtgaat < 4:160299/273‑1 (MQ=255) ttGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCtgaat > 2:318911/1‑274 (MQ=255) cgcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGattat < 5:457649/46‑1 (MQ=255) cgcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGattat > 6:457649/1‑46 (MQ=255) cacaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCAt < 5:313327/109‑1 (MQ=255) cacaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCAt > 6:313327/1‑109 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGGGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCgg < 2:445949/272‑1 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCg < 8:513865/47‑1 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGtt < 5:263658/88‑1 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGtt > 6:263658/1‑88 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa < 6:829722/126‑1 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa > 5:829722/1‑126 (MQ=255) aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCg < 6:1058794/148‑1 (MQ=255) ggTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTa < 2:282200/274‑1 (MQ=255) ggTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTa < 2:315005/274‑1 (MQ=255) ggTTATCATTATTCAGAGGGCGAGATTATTTATCTCGCCGATAGGCCATTCGGGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTa < 2:472295/274‑1 (MQ=255) aTCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa > 4:208630/1‑153 (MQ=255) ctctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGa > 7:479175/1‑148 (MQ=255) ctctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACCGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCAGCAAATGAAGATCTCCTGAATGAAGATTCTACGGCCCCGGTTATCCGCtt > 4:417408/1‑275 (MQ=255) tctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGAc < 7:764761/88‑1 (MQ=255) ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTggt < 6:617774/112‑3 (MQ=255) ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTggt > 5:617774/1‑110 (MQ=255) ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCgg < 2:208631/153‑1 (MQ=255) aTTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc < 8:479175/148‑1 (MQ=255) tCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATg > 7:1133525/1‑87 (MQ=255) tCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATg < 8:1133525/87‑1 (MQ=255) agaaGGAGAGATTATTTATCTAGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATg < 1:143790/275‑1 (MQ=255) gagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAg < 7:148780/89‑1 (MQ=255) gagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGACCAATGc > 1:145044/1‑275 (MQ=255) agGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGc > 2:137525/1‑274 (MQ=255) agGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCCCGTCTGGAAGCGGTCTTCCATCAGAATACCGGCGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAAGGAAAATCTCCTGAACGAAGTTTAAGCGCGACCGGCTTACATCTTTATCAATGc > 2:494919/1‑274 (MQ=255) ggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGAt > 2:357911/1‑275 (MQ=255) cGAGATAAATAATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCt < 8:291291/109‑1 (MQ=255) cGAGATAAATAATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCt > 7:291291/1‑109 (MQ=255) cGAGATAAATAATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGc < 8:181937/113‑1 (MQ=255) cGAGATAAATAATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGc > 7:181937/1‑113 (MQ=255) agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGa < 6:389773/78‑1 (MQ=255) agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGa > 5:247688/1‑78 (MQ=255) ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGa < 5:520303/112‑1 (MQ=255) ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGa > 6:520303/1‑112 (MQ=255) tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg > 5:895643/1‑104 (MQ=255) | TCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAA‑ACACGTTCAACAGTCACGCCCCTGCGCCATCGTCACGGTGAGGCATTCATATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATT > NC_000913/3458146‑3458683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |