| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,620,666 | T→G | 15.9% | L492W (TTG→TGG) | rhsB → | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,620,666 | 0 | T | G | 15.9% | 60.1 / 3.5 | 25 | L492W (TTG→TGG) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Reads supporting (aligned to +/- strand): ref base T (10/11); new base G (2/2); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TAACGTCAGCCACCGGGCCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACC > NC_000913/3620612‑3620908 | gcccgtcaggcccggtggCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGcccc > 5:680945‑M2/19‑82 (MQ=255)gcccgtcaggcccggtggCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGcccc < 6:680945‑M2/64‑1 (MQ=255) cccgtcaggcccggtggCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGCCGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTg > 5:307187‑M2/18‑125 (MQ=255) cccgtcaggcccggtggCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTg < 6:307187‑M2/108‑1 (MQ=255) cgtcaggcccggtggCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTgg > 8:1007620‑M2/16‑54 (MQ=255) cgtcaggcccggtggCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTgg < 7:1007620‑M2/39‑1 (MQ=255) cgtcaggcccggtggCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTa > 8:808779‑M2/16‑106 (MQ=255) cgtcaggcccggtggCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTa < 7:808779‑M2/91‑1 (MQ=255) cgtcaggcccggtggCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTg > 8:276045‑M2/16‑123 (MQ=255) cgtcaggcccgggggCTGACGGGCGGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTg < 7:276045‑M2/108‑1 (MQ=255) TGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCGGGAAACTGCCCCTGATGGCGa > 8:173027‑M2/1‑72 (MQ=255) TGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCGGGAAACTGCCCCTGACGGCGa < 7:173027‑M2/72‑1 (MQ=255) TGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCCCACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCAGGAAAACCATGCCGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCAACTGTTCCGGTTATGTCACCAGTTATGACCCTGCCCGCTTCTGGCAGCTGACGACTGTCCACCGCgcggaagagcaggacaagt > 1:122828‑M2/1‑266 (MQ=255) GACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGtt < 7:806904‑M2/88‑1 (MQ=255) GACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGtt > 8:806904‑M2/1‑88 (MQ=255) GACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATaa > 7:262918‑M2/1‑95 (MQ=255) GACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATaa < 8:262918‑M2/95‑1 (MQ=255) GACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGaaaa > 6:243401‑M2/1‑148 (MQ=255) ACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAAc > 8:270654‑M2/1‑148 (MQ=255) CGGGCTGGAATTGCGCCGGGAATATGATGAATTGGTCCGTCTGATGCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAAcc < 8:236934‑M2/148‑1 (MQ=255) CGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAAcc > 7:552730‑M2/1‑148 (MQ=255) GGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGTAACGGAAGATGCCACCGGCAGCCGGAAAACCa < 6:227526‑M2/148‑1 (MQ=255) GGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCa < 7:803460‑M2/148‑1 (MQ=255) GGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCa < 5:243401‑M2/148‑1 (MQ=255) GGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTAcc < 2:277833‑M2/274‑1 (MQ=255) | TAACGTCAGCCACCGGGCCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACC > NC_000913/3620612‑3620908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |