Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,630,661 A→T 9.1% intergenic (‑59/+307) yhiI ← / ← yhiJ putative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,630,6610AT 9.1% 501.6 / 34.1 155intergenic (‑59/+307)yhiI/yhiJputative membrane fusion protein (MFP) of efflux pump/DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base A (72/67);  new base T (7/7);  total (80/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

Only 100 of 155 total aligned reads displayed.

CGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGT  >  NC_000913/3630392‑3630926
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                         
cgcgAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGCTAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCAAAATCGACCCGCCATAATCCCTGTACGCAAACTACGCGTGTTACCACTGCCCCAGCACGACCtt                                                                                                                                                                                                                                                                       >  4:104815/1‑274 (MQ=255)
cgcgAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGtt                                                                                                                                                                                                                                                                       >  2:359776/1‑274 (MQ=255)
               cttcttTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCCAACGACGCGTGTTACAACTGCCGCCGAACGAAGTTCAGCGGAATCTTccca                                                                                                                                                                                                                                                       >  4:476384/1‑273 (MQ=255)
                ttcttTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCCCGATAGCCGCCACCGCCAGTAACCCGCCCACCCACCACGCCAGATGGCCCTTCCCTCTACCCATAATCCACCAGCCATAACCCCTTTCACCAAACGCCCCTTCTTACCACTACCGCCCACCGACGTCCAACgctatattccac                                                                                                                                                                                                                                                       >  4:366073/1‑263 (MQ=255)
                               ggggctgttgcGCCCGGCAATTTTTCTGGCAATAACCACTTCCGTCGCTTCAATGCCCCCATTACTGACAGCAAAGCCTTCCGGCACACCTTCCGGGCGCACACGCCACCACCCCCCGCTAACCCCCACCCCCAGTCACCCGACCACCCACCACGCCACATGGCGCTTACCCCTCCTCATAAGCCACCCCCCATAATCCCCGTAAACCCACCCCACGTGTTACAACCCGCCCCCCAACACCTTCCGCGGATTCCTCCCCCCGGGATAAAACGGa                                                                                                                                                                                                                                        >  4:416905/11‑274 (MQ=255)
                                      aTACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCa                                                                                                                                                                                                                                 >  2:280859/1‑274 (MQ=255)
                                           cccGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCGTAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGAAAATAc                                                                                                                                                                                                                            >  2:382533/1‑274 (MQ=255)
                                           cccGGCAATTTTGCTGGCAATATCCACCTCCGTCGCTACAATGCTCCCATTACTGATAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCACCACCGCCAGTAACCCGATAACTCACCACGCCCGATGGCGCTTACTCTTATCCATAACCGACCCTCCATAATACCTGTAAGCCAACGACGCGGGTTACCACGGCCGCCGAACGACGATCACCGGATTCTTCAATCAGGGACAAGAAGGAAACGGCAAATACg                                                                                                                                                                                                                           >  4:222194/1‑275 (MQ=255)
                                               gCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCa                                                                                                                                                                                                                       >  2:359167/1‑275 (MQ=255)
                                                                     cTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCa                                                                                                                                                                                                 >  2:495966/1‑275 (MQ=255)
                                                                           tCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCACCAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATGTGGCGCCAACgggg                                                                                                                                                                                           >  2:525852/1‑275 (MQ=255)
                                                                                tCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGc                                                                                                                                                                                      >  4:206500/1‑275 (MQ=255)
                                                                                        cccATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCAACCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCTCAGAGCCTTGAGCGACAACGGGGCAGGCTTAg                                                                                                                                                                                  >  1:89427/1‑271 (MQ=255)
                                                                                        cccATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTCCAGCGGATTCTTCCATCCGGGATAAGATGGAAACGGCAAATACGCCCAGCCCGCTGAGCCTTACGCGCCAACGGGGAAGCCTTACTGaa                                                                                                                                                                              >  2:232131/1‑275 (MQ=255)
                                                                                              aCTAACAGAAAAGCCCCCGGGCACCACCCCCGGGCGCAACAGCAACCCGGCCACGATAGCCGCCACCGCCAGTAACCCGACACCCCACCACGCCAGAGGGCGCGTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACggg                                                                                                                                                                        <  4:280858/275‑1 (MQ=255)
                                                                                                     gCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTaa                                                                                                                                                                  >  2:142629/1‑274 (MQ=255)
                                                                                                     gCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAACGCGCAGACCATTGAGCCCCAACGGGGAAGGCTTAGTACAAACGGGTAGTAAt                                                                                                                                                                 >  4:24507/1‑275 (MQ=255)
                                                                                                                                      accccccaccccacgAGGCCCCCCACCGCCCGAAACCCCACAACCCACCCCGCCCGGTGTCCATTCCTCTTTTCCATAACCGCCCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGa                                                                                                                                <  4:359775/270‑1 (MQ=255)
                                                                                                                                       gCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGa                                                                                                                                                                                                                                                                >  6:140170/1‑146 (MQ=255)
                                                                                                                                       gCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGa                                                                                                                                                                                                                                                                <  5:140170/146‑1 (MQ=255)
                                                                                                                                        ccaccaCGCCACGATACCCGCCCCCCCCATTAACCCAACAACCCACCCCGCCAGATGGCGCTTACTCTTATCCATAACCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGaaa                                                                                                                              <  2:24507/275‑1 (MQ=255)
                                                                                                                                          accacGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGc                                                                                                                                                                                                                                                                   >  8:1114798/1‑140 (MQ=255)
                                                                                                                                          accacGCCACGATAGCCGCCACCCCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGc                                                                                                                                                                                                                                                                   <  7:1114798/140‑1 (MQ=255)
                                                                                                                                               cccgcgATAGCCCCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTACCCAAAATCGCCCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCAt                                                                                                                       <  2:143005/271‑1 (MQ=255)
                                                                                                                                                    gacacccgccccccccaGTAACCCCACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAACCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCa                                                                                                                  <  1:70421/270‑1 (MQ=255)
                                                                                                                                                            ccaccgcaaGTAACCCGACCACCCAACACGCCAGATTGCGCTTACTCTAACACATAACCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGtgtg                                                                                                          <  2:258633/275‑1 (MQ=255)
                                                                                                                                                               ccgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                                                                                                                                                            >  6:292904/1‑142 (MQ=255)
                                                                                                                                                               ccgccaGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                                                                                                                                                            <  5:292904/142‑1 (MQ=255)
                                                                                                                                                                            gACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                                                                                                                                                            <  6:914964/129‑1 (MQ=255)
                                                                                                                                                                             aCAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaaga                                                                                                                                                                                                                                            >  6:43995/1‑128 (MQ=255)
                                                                                                                                                                                     ccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGt                                                                                                                                                                                                                                                                        <  7:1045334/92‑1 (MQ=255)
                                                                                                                                                                                     ccacGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGt                                                                                                                                                                                                                                                                        >  8:1045334/1‑92 (MQ=255)
                                                                                                                                                                                        cGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACg                                                                                                                                                                                                                           >  6:730068/1‑134 (MQ=255)
                                                                                                                                                                                        cGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACg                                                                                                                                                                                                                           <  5:730068/134‑1 (MQ=255)
                                                                                                                                                                                            cgATTGCGTTTACTCTCACCAAGAACCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGTAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatcca                                                                          <  1:153135/274‑1 (MQ=255)
                                                                                                                                                                                              aTGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAAc                                                                                                                                                                                                                                     >  5:243218/1‑118 (MQ=255)
                                                                                                                                                                                              aTGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAAc                                                                                                                                                                                                                                     <  6:243218/118‑1 (MQ=255)
                                                                                                                                                                                                    cTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCa                                                                                                                                                                                                 >  8:742378/1‑148 (MQ=255)
                                                                                                                                                                                                          ctTACCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                                                                                                                                                    <  6:541147/91‑1 (MQ=255)
                                                                                                                                                                                                            tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAgg                                                                                                                                                                                                                                         >  8:341904/1‑100 (MQ=255)
                                                                                                                                                                                                            tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAgg                                                                                                                                                                                                                                         <  7:341904/100‑1 (MQ=255)
                                                                                                                                                                                                            tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCGAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                                                                                                                                                         <  7:742378/148‑1 (MQ=255)
                                                                                                                                                                                                            tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAg                                                                                                                                                                                                                      >  5:528762/1‑119 (MQ=255)
                                                                                                                                                                                                            tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTg                                                                                                                                                                                                        >  8:585850/1‑133 (MQ=255)
                                                                                                                                                                                                            tATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGaa                                                                                                                                                                                         >  8:1072944/1‑148 (MQ=255)
                                                                                                                                                                                                                 aTAATCGACCCGCCATAATCCCTGTACGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                                                                                                                                                    >  6:771925/1‑84 (MQ=255)
                                                                                                                                                                                                                 aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGaa                                                                                                                                                                                                                                       >  8:13404/1‑97 (MQ=255)
                                                                                                                                                                                                                 aTAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATa                                                                                                                                                                                                                             <  6:1065970/107‑1 (MQ=255)
                                                                                                                                                                                                                 aTAACCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCg                                                                                                                                                                                                                                                    <  5:771925/84‑1 (MQ=255)
                                                                                                                                                                                                                  tAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACggg                                                                                                                                                                                            >  5:597609/1‑139 (MQ=255)
                                                                                                                                                                                                                     tCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaagaa                                                                                                                                                                                                                                           >  7:167985/1‑89 (MQ=255)
                                                                                                                                                                                                                     tCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAg                                                                                                              >  4:116011/1‑214 (MQ=255)
                                                                                                                                                                                                                      cGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                                                                                                                                             >  5:1015631/1‑86 (MQ=255)
                                                                                                                                                                                                                      cGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                                                                                                                                             <  6:1015631/86‑1 (MQ=255)
                                                                                                                                                                                                                        aCCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGtt                                              <  2:222195/275‑1 (MQ=255)
                                                                                                                                                                                                                         cccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                                                                                                                                            >  6:342301/1‑148 (MQ=255)
                                                                                                                                                                                                                          ccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                                                                                                                                            >  6:363895/1‑147 (MQ=255)
                                                                                                                                                                                                                          ccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGaaaa                                                                                                                                                                            <  5:363895/147‑1 (MQ=255)
                                                                                                                                                                                                                          ccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCa                                            <  2:206501/275‑1 (MQ=255)
                                                                                                                                                                                                                          ccGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCa                                            >  2:278005/1‑275 (MQ=255)
                                                                                                                                                                                                                           cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGa                                                                                                                                                                                          <  6:469882/132‑1 (MQ=255)
                                                                                                                                                                                                                           cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGa                                                                                                                                                                                          >  5:469882/1‑132 (MQ=255)
                                                                                                                                                                                                                           cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                                                                                                                                                    <  7:615963/138‑1 (MQ=255)
                                                                                                                                                                                                                           cGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                                                                                                                                                    >  8:615963/1‑138 (MQ=255)
                                                                                                                                                                                                                            gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                                                                                                                                             <  8:368093/80‑1 (MQ=255)
                                                                                                                                                                                                                            gCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATaag                                                                                                                                                                                                                                             >  7:368093/1‑80 (MQ=255)
                                                                                                                                                                                                                             ccATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCggg                                                                                                                                                                                                                                                  >  5:1116363/1‑74 (MQ=255)
                                                                                                                                                                                                                               aTAATCCCCTTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGc                                       <  2:104816/275‑1 (MQ=255)
                                                                                                                                                                                                                                     ccTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACg                                                                                                                                                                          <  7:1085101/138‑1 (MQ=255)
                                                                                                                                                                                                                                     ccTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGATCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGc                                                                                                                                                                                                            >  8:702424/1‑104 (MQ=255)
                                                                                                                                                                                                                                       tGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAg                                                                                                              >  4:5683/1‑196 (MQ=255)
                                                                                                                                                                                                                                        gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCa                                                                                                                                                                                                                       <  8:751556/90‑1 (MQ=255)
                                                                                                                                                                                                                                        gTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAAc                                                                                                                                                                                               >  4:26965/1‑114 (MQ=255)
                                                                                                                                                                                                                                           aGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGtgtg                                                                                                          <  2:5683/196‑1 (MQ=255)
                                                                                                                                                                                                                                            gcaaaacgcgcgGGTGAAAACGGCCGCCGAACGCCGGTTAGCGGATTCCTCCATCCGGGATAAAAAGGAAACGGCAAATACGAGCAGCGCGCAGGGCATTGAGCGCCAACGGGGAAGGCGTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCAt                          <  4:398029/267‑1 (MQ=255)
                                                                                                                                                                                                                                            gCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcat                                                                                                                                                         >  5:431386/1‑148 (MQ=255)
                                                                                                                                                                                                                                               aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                                                                                                                                                        >  6:113909/1‑114 (MQ=255)
                                                                                                                                                                                                                                               aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAg                                                                                                                                                                                        <  5:113909/114‑1 (MQ=255)
                                                                                                                                                                                                                                               aaCGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatcat                                                                                                                                                      >  6:672510/1‑148 (MQ=255)
                                                                                                                                                                                                                                                           tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgcgc                                                                                                                                                                                                                 <  8:20140/77‑1 (MQ=255)
                                                                                                                                                                                                                                                           tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAgcgcgc                                                                                                                                                                                                                 >  7:20140/1‑77 (MQ=255)
                                                                                                                                                                                                                                                           tACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                                                                                                                                                            <  5:133160/130‑1 (MQ=255)
                                                                                                                                                                                                                                                            aCAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGc                                                                                                                                         >  7:490062/1‑148 (MQ=255)
                                                                                                                                                                                                                                                             cAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGcc                                                                                                                                        <  8:490062/148‑1 (MQ=255)
                                                                                                                                                                                                                                                             cAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGcc                                                                                                                                        >  5:766139/1‑148 (MQ=255)
                                                                                                                                                                                                                                                               aCTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcatca                                                                                                                                                       <  5:244798/131‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGAAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa      <  2:416906/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGtcat                                                                                                                                                         <  6:265863/125‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCag                                                                                                                                                                                                               >  7:778419/1‑71 (MQ=38)
                                                                                                                                                                                                                                                                    ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                                                                                                                                                   <  6:551073/99‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTa                                                                                                                                                                                   >  5:551073/1‑99 (MQ=255)
                                                                                                                                                                                                                                                                    ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAg                                                                                                                                                                                  <  6:129050/100‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                                                                                                                                                       >  6:164718/1‑111 (MQ=255)
                                                                                                                                                                                                                                                                    ccgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgt                                                                                                                                                                       <  5:164718/111‑1 (MQ=255)
                                                                                                                                                                                                                                                                     cgctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAgcg                                                                                                                                                                                                    >  5:311755/1‑81 (MQ=255)
                                                                                                                                                                                                                                                                      gctgAACGTCGTTCGGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGgtagt                                                                                                                                                                    <  7:608569/109‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gccgAACGACGTTCAGCGGATTCTTCCATCCGGGAGAAAAAGGAAACGCGAAAGACGACCAGCGCGCAGAGCATTGAGCACCAACGGGGAAGGCTTAGTGGAAACGAGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGt  <  4:232130/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ccgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAAtgttgt                                                                                                                                                            <  7:723362/119‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                                                                                                                                                    <  7:817387/94‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cgAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCtt                                                                                                                                                                                    >  8:817387/1‑94 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                         
CGCGAACAAACTTGCCTTCTTTCACCAGAATGGTGTCGATACGCCCGGCAATTTTGCTGGCAATATCCACTTCCGTCGCTTCAATGCGCCCATTACTGACAGCAAAGCCTTCCGGCACACCTGCCGGGCGCAACAGCCACCACGCCACGATAGCCGCCACCGCCAGTAACCCGACAACCCACCACGCCAGATGGCGCTTACTCTTATCCATAATCGACCCGCCATAATCCCTGTAAGCAAACGACGCGTGTTACAACTGCCGCCGAACGACGTTCAGCGGATTCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGT  >  NC_000913/3630392‑3630926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: