Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 443,022 G→A 14.4% intergenic (+25/+29) thiI → / ← yajL tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,0220GA14.4% 162.8 / 16.6 62intergenic (+25/+29)thiI/yajLtRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base G (28/25);  new base A (5/4);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-02

CGTCAATGGCTTCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAATCGGGAAGATA  >  NC_000913/442748‑443291
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                              
cGTCAATGGCTTCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTgg                                                                                                                                                                                                                                                                                >  2:535883/1‑275 (MQ=255)
                   aaCGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGCAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGcc                                                                                                                                                                                                                                                              >  2:494766/1‑274 (MQ=255)
                   aaCGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCTCATGGTCGAATGCCGCGGTTGcc                                                                                                                                                                                                                                                              >  4:320446/1‑274 (MQ=255)
                                       ccGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                                                                                                                                         <  2:304406/275‑1 (MQ=255)
                                         gTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCCTTAACAATGTGAAGGTATATCGCCCGTAATTTGGGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTACTGAATCCGAAACTACTCGtacta                                                                                                                                                                                                                                        >  2:406236/1‑274 (MQ=255)
                                                      aaatgaaatgcggCCACTGTATGTCGACGGGGTTTATGTGTTTTCTCCGCCGTTCTATAAACTGGGCACCAAATTTGNGGCGTCCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGGGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                                                                                                                          <  3:124046/263‑1 (MQ=255)
                                                           atgATAAGCCAAAGAAAGTCGAAGTGATTGATGTGGTGTCTCTGCCGTTCTATAAACTGGGCACCAAAGTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGGGTGAGAGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                                                                                                                                     <  4:93421/273‑1 (MQ=255)
                                                           aaGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                                                                                                                                     <  2:320447/275‑1 (MQ=255)
                                                                                        gATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGGGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc                                                                                                                                                                                        <  4:271436/275‑1 (MQ=255)
                                                                                                 tctcTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATcc                                                                                                                                                                                                                                                        >  2:194074/1‑202 (MQ=255)
                                                                                                  cactGCCGTTCTATAAACTGAGACCCAAATGTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGATACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTGTTTCAc                                                                                                                                                                                <  2:99359/271‑1 (MQ=255)
                                                                                                     tGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGaca                                                                                                                                                                                                                                                    <  4:194073/202‑1 (MQ=255)
                                                                                                                    tGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCTCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCCTCACCCGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCCATa                                                                                                                                                            >  4:104584/1‑275 (MQ=255)
                                                                                                                      aGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATACTTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATaa                                                                                                                                                           >  4:122862/1‑274 (MQ=255)
                                                                                                                      aGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATaa                                                                                                                                                           >  4:40485/1‑274 (MQ=255)
                                                                                                                       gCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTCCTCGTAATAATTATAAATCCCTGCCGACATCACCAGTTTGGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGGCGaaaaat                                                                                                                                                         >  4:459229/1‑273 (MQ=255)
                                                                                                                                ttGGGGATCTAGACCCGACAAAAACCCGGGGGCTGTGGGGTGGGGGCGGGGTGATGGGCCGGCTGCAGGCGGTGTATCTGGGCGAGCAGGGCTTTAACAATGGGAAGGTATATCGCCCGTAATTTGTGGTTTGTAAGTGGCATCTGGTCAGATGCGACGTTGGCCGCATCCGACACTACTCGTAATAATGATAAATCCCTGCCGCCATCACCAGTTGGGATGCCACTTCATGGGATTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                                                                                                                                <  4:174045/275‑1 (MQ=255)
                                                                                                                                ttGGCGAACTCGACCAGGACAAAACCGGGCGGGTGTGGTGGGGGGGGGGGGTGATGGGCCGGCGGCAGGGGCTCTATCTGCGGGGGCAGGGCTTTAACAATTGGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                                                                                                                                <  4:494765/275‑1 (MQ=255)
                                                                                                                                     gATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTTCCGCCATCACCAGTTGTCATGCCACTTCATGGGCTTTTTCACGCCGAACCACCAGGTCAATAATTTTCAGCCCaacatc                                                                                                                                           >  2:18976/1‑271 (MQ=255)
                                                                                                                                            aCCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg                                                                                                                                                                                                                                                                            <  5:799349/139‑1 (MQ=255)
                                                                                                                                            aCCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg                                                                                                                                                                                                                                                                            >  6:799349/1‑139 (MQ=255)
                                                                                                                                                      aaCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGcc                                                                                                                          >  2:106340/1‑275 (MQ=255)
                                                                                                                                                       aCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg                                                                                                                                                                                                                                                                            <  5:1094940/128‑1 (MQ=255)
                                                                                                                                                       aCCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAg                                                                                                                                                                                                                                                                            >  6:1094940/1‑128 (MQ=255)
                                                                                                                                                           ggCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTc                                                                                                                                                                >  2:155765/1‑232 (MQ=255)
                                                                                                                                                           ggCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg                                                                                                                     <  2:40486/275‑1 (MQ=255)
                                                                                                                                                               gctgTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATa                                                                                                                                                            <  4:155764/232‑1 (MQ=255)
                                                                                                                                                                  gtggtgTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATc                                                                                                                                                                                                                              >  4:549248/1‑163 (MQ=255)
                                                                                                                                                                      tgtgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                                                                                                                          <  2:549249/163‑1 (MQ=255)
                                                                                                                                                                           gcgcGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTa                                                                                                     >  2:514410/1‑275 (MQ=255)
                                                                                                                                                                                gggTGATGGGCCGTTTGCAGGGGCTCTTTCTGCGCGGGGAGGGCTTTAACAATGTGAAGGTATATCGCCCTTAATTTTTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGt                                                                                                <  4:514409/275‑1 (MQ=255)
                                                                                                                                                                                             tCTGCAGGCTCCCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTATCATCTGGTCAGATGCCACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCATGCCGTCATCACCAGTTCTGATGCCACCTCATGGGCTTTTGCCAGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCCGCAGCCGTACCCGCCCCCTTGCCGGCTCGCATTTTTCCCTCTGCATCCCAgacg                                                                                    >  4:219205/1‑274 (MQ=255)
                                                                                                                                                                                                gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGAcgc                                                                                >  2:528145/1‑275 (MQ=255)
                                                                                                                                                                                                                            tttAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                                                                                                                                       <  8:477063/96‑1 (MQ=255)
                                                                                                                                                                                                                            tttAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                                                                                                                                       >  7:477063/1‑96 (MQ=255)
                                                                                                                                                                                                                                   aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATccc                                                                                                                                                                                                                            >  4:540379/1‑100 (MQ=255)
                                                                                                                                                                                                                                   aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGATCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACAAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAAGTTTGTCt                                            >  2:118519/1‑275 (MQ=255)
                                                                                                                                                                                                                                       gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgcc                                                                                                                                                                                                                        <  2:540380/100‑1 (MQ=255)
                                                                                                                                                                                                                                              tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATCATTATAAATCCCTGCCGCCCTCACCAGTTGTGATTCCACTTCATGGGCTTTTTCACGCCCAACCAACCGGTCGATAATTTTCAGACCAAATTCGATAGCCGTACCCGGCCCCTGTCTGGTCATCAATTTTACACTTGCCTCCCAGACCACGGCCTTTTCCCTCCCANNNTCTGCGGGTAATGTTGCTTTCACCGTctg                                 >  3:153231/1‑273 (MQ=255)
                                                                                                                                                                                                                                              tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCCCCTCTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                                 >  2:363744/1‑275 (MQ=255)
                                                                                                                                                                                                                                              tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCACTTGGCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                                 >  2:421885/1‑275 (MQ=255)
                                                                                                                                                                                                                                                tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTTGTCAGCAATTTTACCCGTGCATCCCAGACGACGCCCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCggg                                >  2:255927/1‑274 (MQ=255)
                                                                                                                                                                                                                                                tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGa                               <  1:120553/275‑1 (MQ=255)
                                                                                                                                                                                                                                                     cGTAATTTGTGGTTTTTACGTCTCATCTGGTCAGATGCGCCGTTTGTCGCATCCGACACTACTCTTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTGTCACGCCCAACCAACAGGTCGATAAGTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAACTTTGTCTTTCAGCGTCGGGAAGCCg                          <  1:153231/275‑1 (MQ=255)
                                                                                                                                                                                                                                                     cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                                                                                                                                            >  5:22447/1‑98 (MQ=255)
                                                                                                                                                                                                                                                     cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                                                                                                                                            <  6:22447/98‑1 (MQ=255)
                                                                                                                                                                                                                                                      gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTAAGCGTCGGGAAGCCgg                         >  2:318486/1‑275 (MQ=255)
                                                                                                                                                                                                                                                          tttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCggg                                <  4:318485/264‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaa                                                                                                                                                                                                                                 >  5:636172/1‑65 (MQ=255)
                                                                                                                                                                                                                                                                 tttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaa                                                                                                                                                                                                                                 <  6:636172/65‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                                                                                                                                       >  5:172273/1‑90 (MQ=255)
                                                                                                                                                                                                                                                                  ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                                                                                                                                       <  6:172273/90‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                                                                                                                                            <  6:312245/77‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                                                                                                                                            >  5:312245/1‑77 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                                                                                                                                       <  5:386752/82‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                                                                                                                                       >  6:386752/1‑82 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacca                                                                                                                                                                       <  7:193214/114‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacca                                                                                                                                                                       >  8:193214/1‑114 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTc                                                                                                                                                      >  6:718197/1‑131 (MQ=255)
                                                                                                                                                                                                                                                                          cGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTc                                                                                                                                                      <  5:718197/131‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg                                                                                                                                                                                           >  7:1061560/1‑93 (MQ=255)
                                                                                                                                                                                                                                                                           gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg                                                                                                                                                                                           <  8:1061560/93‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ttCTGGTCAGTTGCAACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAATCGGGAAGata  <  2:459230/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                              
CGTCAATGGCTTCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAATCGGGAAGATA  >  NC_000913/442748‑443291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: