Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,920,809 | G→A | 5.4% | P27L (CCG→CTG) | atpF ← | F0 sector of membrane‑bound ATP synthase, subunit b |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,920,809 | 0 | G | A | 5.4% | 501.5 / 13.7 | 150 | P27L (CCG→CTG) | atpF | F0 sector of membrane‑bound ATP synthase, subunit b |
Reads supporting (aligned to +/- strand): ref base G (60/81); new base A (4/4); total (65/85) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-02 |
Only 100 of 158 total aligned reads displayed.
TTCACGGGCACGTTTACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCG > NC_000913/3920535‑3921076 | aCGGTCACGTGTACGCGCGGCGTCAAGTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc < 2:29586/274‑1 (MQ=255) cGGGCACGTTTACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCAGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCa < 1:54106/275‑1 (MQ=255) gCACGTTTACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCTAGGTCAAGGTCCTTATGTGTGCTCGTTTTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGGTTTTCGATGGCTGCCATTCATGGCGGCCAt > 1:126474/1‑275 (MQ=255) gCACGTTTACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATAc > 4:452316/1‑275 (MQ=255) gCACGTTTACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATAc > 2:318511/1‑275 (MQ=255) tttACGCTCGGCTTCAATTTCCGCCTGGGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt < 4:74402/275‑1 (MQ=255) tttACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt > 4:348354/1‑275 (MQ=255) tttACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt < 2:499241/275‑1 (MQ=255) tttACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt < 2:453619/275‑1 (MQ=255) tttACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt < 2:452317/275‑1 (MQ=255) gttCGTCTTCACTTTCCGCCTTGTCCTGGGCCACGATTTTTCTACTTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCNGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg < 3:125796/273‑1 (MQ=255) gCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg < 4:395964/275‑1 (MQ=255) gCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg < 1:115556/275‑1 (MQ=255) gCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg < 2:145806/275‑1 (MQ=255) gCTCGGCTTCAATGTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCGGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg < 2:199355/275‑1 (MQ=255) ggCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTCATGGCGGCCATACGTACTTCATGcagg > 1:39801/1‑274 (MQ=255) ggCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACt > 4:180046/1‑266 (MQ=255) gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGTCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcaga < 2:96569/274‑1 (MQ=255) aCGATTTTAGTACGTTCCTGTTCTGCCTCGCCTTGCGCTTCGTCCAGAGTCTGCGAGCGGCGTTTGTTCGCCTTCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGGTGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc < 2:486576/275‑1 (MQ=255) aCGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc < 4:356487/275‑1 (MQ=255) gCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAg > 2:318447/1‑275 (MQ=255) ccTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACCCGATGGCCTGGCCGAGGCTTGTTTCGtttaac > 4:19794/1‑271 (MQ=255) ccTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCACTGGCCTTTGCAAGGTCAATGTCCTTA‑‑TGTTTTCGTTCTGCGGAAACAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATTGCTGCCATTCATGGCGGCCATACGTCCTTCATGCAGCACATATCGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTTCGTTAAg > 2:544585/1‑274 (MQ=255) gTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATg > 2:363840/1‑273 (MQ=255) tCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCct < 2:33527/275‑1 (MQ=255) aTCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTAGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGTGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAttt < 2:4088/275‑1 (MQ=255) cGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTa < 2:166894/275‑1 (MQ=255) gatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc > 5:947005/1‑148 (MQ=255) tACCTGTGCTGCCGTTTTCGCTGTTTTCAGCTGGTCCGTGGCGCTGGCCTTTGCAAGTTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCTGCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAAAGAGCAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCCGATATTGCTCTTTAAATAAAAGc < 1:153380/275‑1 (MQ=255) aCCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGa > 2:56619/1‑253 (MQ=255) tCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTtact > 4:322247/1‑275 (MQ=255) tCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCCGCCATACGTACTTCa > 5:118697/1‑148 (MQ=255) ttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcaga < 8:1056960/148‑1 (MQ=255) tttttCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGCCCGCCATTAATGGCAGCCa > 7:446691/1‑125 (MQ=255) ttttCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTGTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGa < 2:407530/274‑1 (MQ=255) ttttCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTGCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGa < 2:25032/274‑1 (MQ=255) cAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATc < 2:19794/275‑1 (MQ=255) cggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACaaa > 5:761457/1‑148 (MQ=255) cggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACaaa < 6:761457/148‑1 (MQ=255) cTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAAGCTTACTACACGACAGCGAACATCACGTAcagacccaaa > 4:399704/1‑272 (MQ=255) tttGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg > 5:556093/1‑95 (MQ=255) caaggtcaaggtCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctg < 6:751216/142‑1 (MQ=255) gtcaaggtcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGatcat < 2:348355/274‑1 (MQ=255) tcaaggtcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt > 8:1086168/1‑98 (MQ=255) tcaaggtcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt < 7:1086168/98‑1 (MQ=255) ggtcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt < 4:545445/151‑1 (MQ=255) agtcCTTT‑‑TTGGCTCGTTATGCGGAAGCAAGGACGTACGCAATTTCTTTTTGACGTTGTTCGATGGCTGACATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa < 4:476465/273‑1 (MQ=255) gtcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaa > 8:341502/1‑111 (MQ=255) tcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATa < 8:264875/85‑1 (MQ=255) tcCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATa > 7:264875/1‑85 (MQ=255) tA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg > 5:1052536/1‑138 (MQ=255) tA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg > 5:721770/1‑138 (MQ=255) tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac > 8:580067/1‑94 (MQ=255) cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc < 8:907926/118‑1 (MQ=255) cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGACCCTCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAg > 2:399948/1‑275 (MQ=255) tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg > 6:479059/1‑134 (MQ=255) cGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCaa > 2:229517/1‑201 (MQ=255) cttctgAAGCTATGCTGTCAGCGTTGTTNTNTTGACTTTTTTCGTTTGGTGCCATTAATGTCGGCCATACGTAATTNATGCAGAACAGAACGAACCTGACAAACGCGTTGGCCTGGCCGGGGATTGTTGCGTTAAGATTCACCGCANAATGACTCTATTTAGTTAACGTTCGTATATTCCTGTTTAAATAAAAGCAAAGCTGACTACGCGACAGCGAACATCACGTACAGACCCCGACCCACAGCGATCATCGGGATAGCATCCACCAGACCCAt < 3:51378/270‑1 (MQ=255) cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCt < 4:229516/201‑1 (MQ=255) tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa < 7:983688/95‑1 (MQ=255) gCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATaa < 2:325071/275‑1 (MQ=255) aaGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGa < 5:1047/102‑1 (MQ=255) aaGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGa > 6:1047/1‑102 (MQ=255) aGCAAGGCCGTCAGCAATTTCTTTGTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACGTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATAGTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTAc < 1:147906/221‑1 (MQ=255) cAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa > 6:625460/1‑87 (MQ=255) aGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAtt > 5:386932/1‑148 (MQ=255) ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg < 8:239401/117‑1 (MQ=255) aTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGGCAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTc < 8:629838/148‑1 (MQ=255) ttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg < 7:1099969/96‑1 (MQ=255) ttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGca > 7:951052/1‑121 (MQ=255) cTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc < 5:302209/85‑1 (MQ=255) tttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGata < 6:947005/148‑1 (MQ=255) ttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGatat > 5:871375/1‑148 (MQ=255) ttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAt > 8:382416/1‑107 (MQ=255) ttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAt < 7:382416/107‑1 (MQ=255) cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAt < 5:256470/89‑1 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcct > 6:341583/1‑77 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgt > 7:66568/1‑90 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgt < 8:66568/90‑1 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGt < 5:248085/95‑1 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGt > 6:248085/1‑95 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTa > 6:473502/1‑128 (MQ=255) tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTa < 5:473502/128‑1 (MQ=255) tttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGCCCCATAACGATAAAGAACTGAGTACGAAGCAACGGAatcagat > 4:243643/1‑274 (MQ=255) aTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa > 6:289994/1‑113 (MQ=255) aTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa < 5:289994/113‑1 (MQ=255) ggCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAt < 7:265487/104‑1 (MQ=255) ggCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGa > 7:608226/1‑128 (MQ=255) gCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATc > 2:61295/1‑216 (MQ=255) cTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgctg < 6:274347/81‑1 (MQ=255) gCCATTAATGGCGGCCATACGTACTTCATGGAGAACAGAACGAACAGGACAAACGCGa < 7:946844/58‑1 (MQ=255) gCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGa > 8:946844/1‑58 (MQ=255) gCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTa > 6:242250/1‑111 (MQ=255) ccATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc < 4:61294/216‑1 (MQ=255) ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCctc > 7:844605/1‑101 (MQ=255) ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTg < 5:654003/125‑1 (MQ=255) ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTg > 6:654003/1‑125 (MQ=255) ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGct < 8:965494/127‑1 (MQ=255) taagtgcggcAATACGTACGTCATGCAGAACAGAACGAACAGGACAAACGCTATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg < 1:9715/270‑1 (MQ=255) gcggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTAcg > 6:1068587/1‑148 (MQ=255) | TTCACGGGCACGTTTACGCTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTA‑‑TGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCG > NC_000913/3920535‑3921076 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |