Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,963,415 | A→C | 7.7% | L272R (CTG→CGG) ‡ | gpp ← | guanosine pentaphosphatase/exopolyphosphatase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,963,415 | 0 | A | C | 7.7% | 565.6 / 34.8 | 169 | L272R (CTG→CGG) ‡ | gpp | guanosine pentaphosphatase/exopolyphosphatase |
Reads supporting (aligned to +/- strand): ref base A (85/71); new base C (6/7); total (91/78) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.78e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01 |
Only 100 of 170 total aligned reads displayed.
CTGACAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATTCATCGGCAACCGGACGTAACACTTCGCGTGCCGCTTTTTCTGCAGCATCA > NC_000913/3963150‑3963684 | cTGACAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCATGGTCATCCACTGAATATTCAGTTCGGTAAAAATGGCCATCAGGATCGCCa > 2:443656/1‑275 (MQ=255) ggACAGGCGCTGATGAGCAAATCGCGGCTTATTGTTTCAAGATGCCATTCGTTGTCCACCGGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCa < 1:15948/273‑1 (MQ=255) aCAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGa > 4:173935/1‑274 (MQ=255) aCAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGACCGCCCGGGTCATACACTGAATATTCAGTTAGGTAAAAATGGCGACCAGGATCGCCATAc > 2:40627/1‑275 (MQ=255) cAGGCGCTGATTAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGAc < 1:68046/274‑1 (MQ=255) gtctggtgggaaaaTAGCGACTTATTGGGTAAAGATGCCATTCCGTTTCCACCTAGTCGAGGAAGTTAGACGAAACTTTGGCTACTCGCTGTGCCTAATCAATATCGATCATAAATCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTc < 4:297152/264‑1 (MQ=255) cAAGATGCCATTCGTTTTCCACCTGATCGAAGCAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTATGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCATGCAGCGCACCGCCCGCCAGGGCCCTACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGAACGCCATACCACCCGGGACCACTAACCCACTTTCCACCgcac > 1:42423/1‑270 (MQ=255) ttAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTACCGCAGCGCACCGCCCGCCAGGGGCATACACGGAATATTCAGTTCGGGAAAAATGGCCAACAGGATAGCCAGACCACTCGGGAACACTAACGCCAGTTCCAGCGTAAGCCCGTCAATCCCCAGTTTCCCCAGCAGACACCaatg > 4:295739/1‑275 (MQ=255) aGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga > 2:530616/1‑274 (MQ=255) aaCTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCt < 2:315220/274‑1 (MQ=255) tGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATAGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCCATCTCCAGTTCCTCCAGCCGCCCGCAATGAATGGCTCGCTg > 2:74723/1‑274 (MQ=255) gggCCTGATCAATTTCGAACATAAAGCGGCGTTTAAGATTACGCAGCGAACGGCTGCGAATATCCGGCTCGACGGCAAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACGCTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgctgc < 4:152380/273‑1 (MQ=255) atatCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATTCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCACCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCa > 2:463752/1‑274 (MQ=255) atatCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCa > 2:4201/1‑274 (MQ=255) cGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCggg < 2:11834/173‑1 (MQ=255) gATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCACGACGGCCATATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTACAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTa < 2:60822/274‑1 (MQ=255) aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGCATGGCCCGCTGTTTCCATTGCTGCAACTTTTCCAGGGTaa > 1:131558/1‑274 (MQ=255) aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTaa > 2:30871/1‑274 (MQ=255) aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCAAGGGTaa > 2:25330/1‑274 (MQ=255) aTAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCa < 6:264466/148‑1 (MQ=255) aTAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCg < 2:262325/275‑1 (MQ=255) aaggcggcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGtt < 2:176850/272‑1 (MQ=255) aaaGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTtcctcc > 2:430375/1‑214 (MQ=255) aaaGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGTTAATGCGtt > 2:528897/1‑275 (MQ=255) ggcgTTGAATATCACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTcatcc < 2:295740/275‑1 (MQ=255) aaTATTACGCACGGTACGGCTGGGAATACCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATccc < 2:362593/273‑1 (MQ=255) atTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTc > 8:319089/1‑148 (MQ=255) ttACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCg < 7:319089/148‑1 (MQ=255) cAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGAAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCcatc < 1:87094/274‑1 (MQ=255) aGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATNGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCCCCAGTTCCTCCAGCCGCNCGCCATGACTGGCCCGCTGTTTCCATTCCTCCACCTTTTCCAGGGNANAGCCTTCATCCATccctttccctttta > 3:41414/1‑263 (MQ=255) tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCgg < 5:838692/139‑1 (MQ=255) tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACTAACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCTCTGTTTCAATTGCTGCAACTTTTCCAGGGTAGAGCGTTCCTCCATCCCCtgtcttctcaacat > 4:318428/1‑263 (MQ=255) tccGAATATCCTGCTCGACGGCCAGAGGAAACAGGCCGTAGACCAGGCCTTCAGGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGt < 2:243724/273‑1 (MQ=255) cGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTTCCTTCATGATTTCCTTTa > 4:342246/1‑274 (MQ=255) cGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTa > 2:215963/1‑274 (MQ=255) cTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTc > 6:386647/1‑148 (MQ=255) gCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGa < 5:167314/105‑1 (MQ=255) ccAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAt < 7:971912/148‑1 (MQ=255) tGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCa < 8:942454/97‑1 (MQ=255) tGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCCTGATCGCCat < 7:252253/91‑2 (MQ=255) ccGTAAACCCGCCCTTCACGCAGCGCCCCGCCCGCCAGGGTCATACCCTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGcaca < 4:4201/274‑1 (MQ=255) cGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatgaat > 4:354333/1‑163 (MQ=255) gACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCt < 2:354334/163‑1 (MQ=255) cAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCg < 4:42448/144‑1 (MQ=255) aGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGa < 8:1114821/91‑1 (MQ=255) aGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGc < 5:386647/148‑1 (MQ=255) ggCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAACAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTTCCATCATGATTTCCTGTACCCCCTCCCCGGTGCCGGAACCCCCAACGCACCCTTTCCAccc > 4:438963/1‑274 (MQ=255) gCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGt > 2:288456/1‑275 (MQ=255) ttcaccgaacacACCGCCGGCCACGGGCAACCACTTAATATCAACGTCGGAAAAAATGGCGAGCAGTATCGCCAGACCACACGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCCATTCCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTTCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGGTTTCGTGTGACGCCTGCACGGTGCCGTAAGCACCAACGCATACTTTCCCGCCGTGa < 4:74722/263‑1 (MQ=255) ttCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga < 5:638443/148‑1 (MQ=255) ttCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga > 5:322979/1‑148 (MQ=255) tCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGt < 4:455519/225‑1 (MQ=255) cGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGtt > 6:769295/1‑97 (MQ=255) gCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga > 6:520604/1‑143 (MQ=255) gCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga < 5:520604/143‑1 (MQ=255) gCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga > 8:723745/1‑143 (MQ=255) gCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga < 7:723745/143‑1 (MQ=255) ccgcccgcCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCg > 6:598236/1‑129 (MQ=255) cccgccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTg > 7:88104/1‑143 (MQ=255) gccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAAtt < 6:965100/148‑1 (MQ=255) ccAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtg < 6:322979/148‑1 (MQ=255) tACACTGAATATTCAGTTCGGTAAAAATGGTGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAg > 8:700336/1‑76 (MQ=255) tACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga > 7:603175/1‑119 (MQ=255) tACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatga < 8:603175/119‑1 (MQ=255) acacTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAtct < 6:444778/92‑1 (MQ=255) acacTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAtct > 5:444778/1‑92 (MQ=255) tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGttt < 7:526061/128‑1 (MQ=255) tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGttt > 8:526061/1‑128 (MQ=255) tGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCCCACTTTCCAGCCGTGATACCGTAATTCATCGCCACCCGGACGTAACCCTTc > 4:451590/1‑275 (MQ=255) gAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAt < 7:239548/85‑1 (MQ=255) gAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAAt > 8:239548/1‑85 (MQ=255) aaTATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAAt > 4:119761/1‑130 (MQ=255) aaTATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTACCGCCGGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATCCCTCGGCAACCGGACGTAACACTTcc > 4:360054/1‑273 (MQ=255) aaTATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATTCATCGGCAACCGGACGTAAAACTTcg > 1:122093/1‑274 (MQ=255) aaTATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGAAACCGTAATTCATCGGAAAACGGAAGTAACAATTcgc > 4:379647/1‑275 (MQ=255) atTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGa < 5:421699/47‑1 (MQ=255) atTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGa > 6:421699/1‑47 (MQ=255) atTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgc < 7:267719/130‑1 (MQ=255) ttCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgct < 2:119762/130‑1 (MQ=255) tCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCa < 5:205694/132‑1 (MQ=255) tCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCa > 6:205694/1‑132 (MQ=255) gcAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATTCATCGGCAACCGGACGTAACACTTCGCGTGcc < 2:342247/274‑1 (MQ=255) ttCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatgaat < 8:276793/105‑1 (MQ=255) ttCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGt > 5:510387/1‑148 (MQ=255) ttCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGt < 6:67789/148‑1 (MQ=255) tAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgc > 5:763346/1‑119 (MQ=255) tAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgc < 8:254337/119‑1 (MQ=255) aaaaaTGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTcatcc > 8:442556/1‑148 (MQ=255) aTGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATg > 2:96900/1‑135 (MQ=255) aTGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCg < 6:810813/137‑1 (MQ=255) aTGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCg > 5:810813/1‑137 (MQ=255) tGGCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCaatgaa > 7:664141/1‑93 (MQ=255) tGGCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGc < 8:779355/101‑1 (MQ=255) ggCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATtgc > 8:637126/1‑112 (MQ=255) gCGATCCTGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTtcct > 6:1013288/1‑73 (MQ=255) cGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGtt < 4:96899/135‑1 (MQ=255) gATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCtgt < 6:510387/148‑1 (MQ=255) gATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATTCATCGGCAACCGGACGTAACCCTTCGCGGGCCGCTTTTTCTGCAGCCTcc > 4:213200/1‑273 (MQ=255) gATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATTCATAGCCACCCGGACGTAACCCTTAGCGTGCCGCTTTTTCTGCAGCAACa > 2:381137/1‑274 (MQ=255) aTCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAg < 7:748717/50‑1 (MQ=255) | CTGACAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTTCCAGCGTCAGCCCGTCAATCTCCAGTTCCTCCAGCCGACCGCAATGAATGGCTCGCTGTTTCAATTGCTGCAACTTTTCCAGGGTAATGCGTTCATCCATCCCCTGTGCCATCATGATTTCCTGTAACGCCTGCACGGTGCCGGAAGCACCAACGCACACTTTCCAGCCGTGATACCGTAATTCATCGGCAACCGGACGTAACACTTCGCGTGCCGCTTTTTCTGCAGCATCA > NC_000913/3963150‑3963684 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |