Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 457,659 | T→A | 5.4% | V78V (GTT→GTA) | clpX → | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 457,659 | 0 | T | A | 5.4% | 456.4 / 10.6 | 127 | V78V (GTT→GTA) | clpX | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease |
Reads supporting (aligned to +/- strand): ref base T (52/67); new base A (4/3); total (57/70) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.99e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01 |
Only 100 of 129 total aligned reads displayed.
ACAAAGAACAAAGAAGAGGTTTTGACCCATGACAGATAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAA > NC_000913/457398‑457929 | agGTTTTGACCCATGACAGATAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTACCGGCCAGGAACAGGCGAAAAAAGtcat > 4:511189/1‑272 (MQ=255) agGTTTTGACCCATGACAGATAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGAAACCACCTGGCCGCTTAACTTATCGGCCAGGAACAAGCAAAAAAAGTgag > 4:251143/1‑273 (MQ=255) aCCCATGACAGATAAACGCAAAGATGGCTCAGGGAAATTGCTTTATTGCTCTTTGTGCGGCAAAAGCCAGCATGAAGTGCGCAAGTTGATTGCCCGTCCATCCGTGCATATCTGCGACGAATGTGTTGATTTATGTCCCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg < 2:511190/275‑1 (MQ=255) aCCCATGACAGATAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg < 2:221800/275‑1 (MQ=255) aCCCATGACAGATAAAACCAAATGTGGCTCAGGTAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGGCAGCATTAAGTTCGCAAGCTGATTGCCGGTCCATCCTTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcg < 1:101269/275‑1 (MQ=255) aTGACAGATAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGt > 2:190959/1‑275 (MQ=255) aGAAAAACGAAAAGATGGCTACGGCAAANTNGTGTTTTTCAGTTTTTGGGGCAAAAGCCAGCATGAAGGGCGCAAGNTGATTGCCTGTCCATCCGTGTAAATCTGCGACGAATGTGGTGATTTATGTAACGACATCAGTCGCGAAGNGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGAATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACa < 3:38072/275‑1 (MQ=255) aTAAACGCAAAGATGGCTCAGTCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAAc < 2:486026/275‑1 (MQ=255) aTAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAAc < 4:444184/275‑1 (MQ=255) aTGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGATGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGt < 2:99136/275‑1 (MQ=255) gCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCTGTCCATCCGTGTATATCTGCGACGCATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTGATCGGCCAGGAACAGGCGAAAACCGTGCTGGCCGTCGCGGTAACCAACCATTACAAAACTCTgctcca > 4:111542/1‑270 (MQ=255) tgttGTATTGGTCTTTTTGGGGCAAAAGCCAGCATGAAGGGCGCAAGCTGATTGCCGGTCCAGCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGAt < 4:527442/272‑1 (MQ=255) gTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCa > 2:288100/1‑275 (MQ=255) gTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCa > 2:360675/1‑275 (MQ=255) ctctTTTTGCGTCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTCAAGAAGTTGCACCGCCTCGTGAACGCAGTCCGCTACCAACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTAGCGGCCAGGAACAGGCGAACAAAGTGCTGCGGGTCGCGGTATACAACCATTCCAAAAATCTGCCCATCGGCGATCCCACCAATg > 4:69985/1‑275 (MQ=255) tttGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCAt > 4:394990/1‑236 (MQ=255) cGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACa < 2:394991/236‑1 (MQ=255) aaaGCAAGCATGAAGTGCGCAAGTTGATTGCCTGTCAATCCGTGTATATCTGAGACGAATGTGTTGATTTATGTAAAGACATCAGTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGc < 4:89739/275‑1 (MQ=255) aGCCAGCAGGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGGGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCaa < 4:110399/275‑1 (MQ=255) tGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATATGCGACGAATGTGTTTATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACa < 2:139387/275‑1 (MQ=255) tgaGTGCGCAAGCTGTTGGCCGGTCCATCCGTGTATATCTGGGAAGAATGTTTTGATTGTTGTAACGACATCATTCGCGAAGGGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACa < 4:154637/272‑1 (MQ=255) ggTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCg > 8:626162/1‑148 (MQ=255) ccATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTc < 4:288099/275‑1 (MQ=255) aTCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTcc < 5:158558/146‑1 (MQ=255) tgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACa < 6:369718/148‑1 (MQ=255) gtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCgg > 6:1124758/1‑138 (MQ=255) gtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCgg < 5:1124758/138‑1 (MQ=255) cTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGaaa < 7:1132759/148‑1 (MQ=255) cTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGAGTCGAGTTGGGCAAAAGTAACATTCTGCTGAACGGTCCGACCGGTTCCGGTAAAACgctgc > 2:352225/1‑275 (MQ=255) acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGt < 7:626162/148‑1 (MQ=255) aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGcc < 7:1021941/125‑1 (MQ=255) aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGcc > 8:1021941/1‑125 (MQ=255) ttGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGt > 5:652704/1‑148 (MQ=255) tGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGaaaaa < 5:933043/135‑1 (MQ=255) aTTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcgc < 6:652704/148‑1 (MQ=255) tttATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAg < 6:881717/124‑1 (MQ=255) tGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCct > 4:162892/1‑275 (MQ=255) tGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCCAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGGTCCGGTAAACCGCAGCTGGCTGAAAcctttccctttt > 4:312408/1‑263 (MQ=255) aCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGGGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACgctg < 2:312409/248‑1 (MQ=255) cGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCCTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCctgctg > 4:68663/1‑274 (MQ=255) aCATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAACCGTCTGCGCAACGGCGATACCAGCAATGGCGCCGAGTTGGGCAAAAGTAACCTTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGCCTCAAACGCTGGCGCGCccgcgg > 4:286006/1‑270 (MQ=255) tcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCTGCATGAAATTCGTAACCACCTGGACGCTCACGTTATCGGCCAGGAACAGGCGAAAGAAGTGCTGGCGGTCGCGGTATACAACCATTACAAAAGGCTGCGCAACGGCGATAACCGCAATTGCGTCGGCTTGGGCAAACGTAACATTCAGCTGACCGCTTCGACCGGTCGCGGTAAAACGCTGGCGGCTGTAACG‑TGgcgcgcgccgctgggtttt > 4:276/1‑262 (MQ=255) tcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAACGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGt > 2:41980/1‑274 (MQ=255) ttCGCGAATAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGACGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCg < 2:111543/275‑1 (MQ=255) tCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGcc < 5:708475/98‑1 (MQ=255) agaTTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCt < 5:1019656/112‑1 (MQ=255) ttAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCaa > 5:991418/1‑148 (MQ=255) aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACa > 7:601705/1‑123 (MQ=255) aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACa < 8:601705/123‑1 (MQ=255) agaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCt < 7:571818/138‑1 (MQ=255) agaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCt > 8:571818/1‑138 (MQ=255) gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcgc > 5:559113/1‑113 (MQ=255) gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAAGAACAGGCGAAAAAAGTGCTGGCGGTcgc > 7:340003/1‑113 (MQ=255) gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAAGAACAGGCGAAAAAAGTGCTGGCGGTcgc < 8:340003/113‑1 (MQ=255) aagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaa > 8:20691/1‑130 (MQ=255) aagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaa < 7:20691/130‑1 (MQ=255) ttGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAgg < 7:30473/87‑1 (MQ=255) ttGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAgg > 8:30473/1‑87 (MQ=255) ttGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGc < 7:761701/99‑1 (MQ=255) ttGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTTCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGAc > 2:216371/1‑274 (MQ=255) tggCACCGCATCGTGAAGGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGc > 8:761701/3‑99 (MQ=255) aCCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTCCAgg > 6:916964/1‑77 (MQ=37) aCCGCATCGTGAACGCAGGGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTCCAgg < 5:916964/77‑1 (MQ=37) gTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGt < 8:125640/148‑1 (MQ=255) aaCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAg < 2:69986/273‑1 (MQ=255) aCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAg < 6:991418/148‑1 (MQ=255) cGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGt > 7:1047284/1‑148 (MQ=255) gcgcTACCGGTGCCTCATGAAATTCGCCACCACCTGGCCGATTACGTTATCTGACAGTAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACCACCGTCTTCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg < 1:14644/274‑1 (MQ=255) gcgcTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg < 2:162893/274‑1 (MQ=255) aCCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGt > 7:181338/1‑148 (MQ=255) aCGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCaaa < 6:594858/141‑1 (MQ=255) atagAAATCCGGAACAACCTGTACGAGTACGTTATCGGCCAGGAACAGGAGAAAAAAGTGCTGGCGTCCGCGTGATAGAACAAATACAAACGTCTGCGCAACGGCGATACCAGCAAGTGGGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGa < 4:5474/272‑1 (MQ=255) tGAAATTCGCAACCACCTGGACGATTACGTTCTCGCCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctgctg > 5:726815/1‑148 (MQ=255) gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGa < 8:1047284/148‑1 (MQ=255) gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGa > 5:563140/1‑148 (MQ=255) aaaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCa < 6:1036291/108‑1 (MQ=255) aaaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCaa < 5:1094888/112‑1 (MQ=255) aaaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCaa > 6:1094888/1‑112 (MQ=255) aaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTgg < 1:107836/203‑1 (MQ=255) aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCa < 7:1134311/109‑1 (MQ=255) aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCa > 8:1134311/1‑109 (MQ=255) aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGt < 6:270835/121‑1 (MQ=255) gCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCCTGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCAtt > 2:66668/1‑275 (MQ=255) caccTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCg < 8:181338/148‑1 (MQ=255) ccTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACgg < 5:549501/87‑1 (MQ=255) ccTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACgg > 6:549501/1‑87 (MQ=255) cTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGtt < 6:563140/148‑1 (MQ=255) ggACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGAt < 7:1022401/88‑1 (MQ=255) ggACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGAt > 8:1022401/1‑88 (MQ=255) ggACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa < 5:999878/89‑1 (MQ=255) ggACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa > 6:999878/1‑89 (MQ=255) ggACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCTTTCACCATGGCCGACGCGACTACACTCCCCGAAACCGGTTTGGTGGGTTAAGACGTTGAAAACATCATTccaacagtg > 4:181302/1‑266 (MQ=255) aCGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTGGAGTTGGGCaaa < 8:735065/113‑1 (MQ=255) aTTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAcc > 7:926214/1‑86 (MQ=255) aTTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAcc < 8:926214/86‑1 (MQ=255) ttACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATc < 6:279250/127‑1 (MQ=255) ttACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATc > 5:279250/1‑127 (MQ=255) aCGTTATCGGCCAGGAACAGGCGAAAAAAGTCCTTGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGaaa < 2:252614/275‑1 (MQ=255) gTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACgc < 6:726815/148‑1 (MQ=255) ttATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGa < 4:383976/244‑1 (MQ=255) | ACAAAGAACAAAGAAGAGGTTTTGACCCATGACAGATAAACGCAAAGATGGCTCAGGCAAATTGCTGTATTGCTCTTTTTGCGGCAAAAGCCAGCATGAAGTGCGCAAGCTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAA > NC_000913/457398‑457929 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |