Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,227,550 G→A 12.9% intergenic (+39/‑181) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,227,5500GA12.9% 228.2 / 24.5 87intergenic (+39/‑181)metH/yjbBhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base G (32/43);  new base A (5/6);  total (38/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-02

CCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAA  >  NC_000913/4227276‑4227797
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       
ccaTCTGGAAGCTGCTGGAAGTGGAAAAACAACCTGGCATGAAACTCACAGAACCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCGGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgcg                                                                                                                                                                                                                                                         <  2:187733/275‑1 (MQ=255)
      gggAGCTGCTGGAAGGGTAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAAt                                                                                                                                                                                                                                                   <  2:142741/275‑1 (MQ=255)
        gAGGTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTTGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAGGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGa                                                                                                                                                                                                                                                 <  2:378316/275‑1 (MQ=255)
               gcgaaagagAAAAAAACACTGANNAATGANACTCAGGGAATTCTTGCCCATTAGGCCCGGGTCGTGGGTGTGGGTTTGGTACTTCAGCCACCCGGACAGCAAGTTCTACGCTGAAGCACAAATTAAGCGCGATCAGGTGGAAGGTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGcc                                                                                                                                                                                                                                            <  3:21352/267‑1 (MQ=255)
                 taaGTGGGCAAAAACCCTGGCATGAAACCCAACGAAACTTTCGCCAGGTGGGCCGGTGCATCGGTTTCGGGTTGGTCCTTCAGCCACCCGGACAGCAATTACTACGCTGTAGCACAAATTCAGAGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  4:34556/274‑1 (MQ=255)
                        aaaaaCACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATTACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCCCTCCATGAGTGACCTGACTCttaa                                                                                                                                                                                                                                 >  4:351559/1‑273 (MQ=255)
                                 cTGGCATGAAACTCACAGACTCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                                                                                                                        <  2:458449/275‑1 (MQ=255)
                                  tGGCCTTAAACTCTCAGAGTCTTTCGCCCTGGGGCCCGTTCTATCCGTTGCGGGTTGGTCCTTCAGCCGCCCGGACAGCATGTACTCAGCTATAGCACAAATTCAGCGCTATAAAGTTGAAGATTATGCCCGACGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTAGCTGGCACCGAATCTGGGATATGACGCAGACTGATTCACAAATCTGTCACTTTTACTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                                                                                                                        <  4:31226/274‑1 (MQ=255)
                                         tGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAACTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTACAGGTATGAGCGTTACCGCAGTTGAGCGCTGGCTCGCACCTAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAACCAGTCCGCTCAATGAGTGCCCTGTCTCTTAATTAATCTGaccacct                                                                                                                                                                                                                  >  1:7906/1‑269 (MQ=255)
                                                    gAATCTTTTGCCATTTGGCCCGGTGCATTGGTTTGGGGTTGGTACTTCCGCCACCCGGACAGAAAGTACTACGGTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTGCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                                                                                                                       <  2:117013/275‑1 (MQ=255)
                                                     aaTCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                                                                                                                       <  2:299483/274‑1 (MQ=255)
                                                        atttgcccAGGTGGCCCCGTGAATCGGTTGCTGGTTGGTACCTGAGCCACCCGGAAATCCAATAAGTCGCTGTAGCACAAATGCAGCGAAGTCAGGTTGAAGATTTTGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAgg                                                                                                                                                                                                   <  4:384643/269‑1 (MQ=255)
                                                          ttCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGGCACTTTCCCTTACAACAAACAGGGCGCCCAATGAGGTGCCTGTCTCTTTATTAATATGAAACACTTTTCATGGAAACAGGCt                                                                                                                                                                                                 >  4:117012/1‑275 (MQ=255)
                                                                                     tCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGGCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACCCTTATACTGGACACAGGCTGGAATAATCTTAGCCGGAAATACGGAt                                                                                                                                                                      >  2:482289/1‑275 (MQ=255)
                                                                                           tgggctttCAGCCACCCGGACAGCAAGTATTACGCTGTGGCTCAAATTCAGGGCGCTAAGGTTGAAGTTTAGGCCCGCCTTAAAGGTATGAGCGTTACCGAAGTTGCGCGCTGGCTGGCACCGAATCTTGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACGTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaa                                                                                                                                                                <  4:482288/269‑1 (MQ=255)
                                                                                                          ccgacaaGCAAGTCGGACGCTGTAGGAAAAATTAAGCGCGATCAGGTGGAAGATTATGGCCGCCGTAAAGGTATGAACGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTAACGTTTCCTTACAACAAACAAGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGAt                                                                                                                                                 <  4:492705/269‑1 (MQ=255)
                                                                                                                            gCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGCAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTTCTTACAACAAACACGGCGCTCAATGAGTTCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCGGGAATAATCTTAGCCGGCAAAACGAATGTCAAAAGGCACAGTCAAGATGCCAGTCAGCGTGTTTcc                                                                                                                               >  1:20923/1‑275 (MQ=255)
                                                                                                                            gCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTcc                                                                                                                               >  2:211415/1‑275 (MQ=255)
                                                                                                                                        aTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATg                                                                                                                                                                                                                                                  <  7:675729/148‑1 (MQ=255)
                                                                                                                                         ttCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGa                                                                                                                                                                                                                                                 <  8:876247/148‑1 (MQ=255)
                                                                                                                                               gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGccc                                                                                                                                                                                                                                           >  7:78799/1‑148 (MQ=255)
                                                                                                                                               gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGccc                                                                                                                                                                                                                                           <  5:980404/148‑1 (MQ=255)
                                                                                                                                                  aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                <  5:196748/140‑1 (MQ=255)
                                                                                                                                                  aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                >  6:196748/1‑140 (MQ=255)
                                                                                                                                                  aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  8:78799/148‑1 (MQ=255)
                                                                                                                                                    cAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGATTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    <  5:730134/134‑1 (MQ=255)
                                                                                                                                                    cAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    >  6:730134/1‑134 (MQ=255)
                                                                                                                                                        ttggagattgatgccgccgTAAGGGTATGAGCGTTACCTAAGTTGGGCGCTGGCTGGCACCAAAACTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAgg                                                                                                   <  4:211414/262‑1 (MQ=255)
                                                                                                                                                        ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  8:743664/138‑1 (MQ=255)
                                                                                                                                                        ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  7:743664/1‑138 (MQ=255)
                                                                                                                                                         tgaagaagttGCCCGCCGAAAAGGGATGAGCGTTACCGCAGTTGAGGGATGCGTGGAACCGAATCTGGGGTATGACAGGGAATGCATCAAAAAGCTGTCACTTTTCCTTACAACAAAAAGGGCGCTCAAGGAGTGACCTGGCTCATTATTAATAAGAAACACTTATACTGGAAACAGGCTGGAATAAGCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGa                                                                                                  <  4:410841/266‑1 (MQ=255)
                                                                                                                                                                  tGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaa                                                                                                                                                                 >  4:303750/1‑203 (MQ=255)
                                                                                                                                                                      cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAgg                                                                                                                                                             <  2:303751/203‑1 (MQ=255)
                                                                                                                                                                      cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  6:1006957/1‑124 (MQ=255)
                                                                                                                                                                      cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  5:1006957/124‑1 (MQ=255)
                                                                                                                                                                              aGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                <  6:535035/112‑1 (MQ=255)
                                                                                                                                                                              aGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                >  5:535035/1‑112 (MQ=255)
                                                                                                                                                                                     aGCGTTACCGAAGTTTAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACt                                                                      <  2:351560/275‑1 (MQ=255)
                                                                                                                                                                                          tACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCgtg                                                                  >  2:373777/1‑274 (MQ=255)
                                                                                                                                                                                           aCCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTAATTCCATTGCTTCAGGAACATATAACGATAAGGAAAACCTGACCTCgttt                                                                 >  4:262684/1‑272 (MQ=255)
                                                                                                                                                                                                         ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCAc                                                   >  4:75974/1‑274 (MQ=255)
                                                                                                                                                                                                                gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTg                                                                                                                                                                                                                                         <  5:467346/85‑1 (MQ=255)
                                                                                                                                                                                                                gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTg                                                                                                                                                                                                                                         >  6:467346/1‑85 (MQ=255)
                                                                                                                                                                                                                   ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTctct                                                                                                                                                                                                                                    <  6:928526/87‑1 (MQ=255)
                                                                                                                                                                                                                   ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTctct                                                                                                                                                                                                                                    >  5:928526/1‑87 (MQ=255)
                                                                                                                                                                                                                   ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  8:419172/1‑79 (MQ=39)
                                                                                                                                                                                                                   ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  7:419172/79‑1 (MQ=39)
                                                                                                                                                                                                                    cGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    <  6:526237/70‑1 (MQ=255)
                                                                                                                                                                                                                    cGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    >  5:526237/1‑70 (MQ=255)
                                                                                                                                                                                                                              gTATGACTCGGACTGTTTCACAAATCTGTCACTTTTCCTTAAAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGAACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCctg                              <  4:373776/274‑1 (MQ=255)
                                                                                                                                                                                                                                       ggACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAANGTCAAAAGGCACAGTAAAGATGACAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGATTAAGGAANATATAAAGATAAGGAGAACATGAACGAGTGTGAAAGCGACTTCAANCNCGTTCGGACGTAGACCTGCTGGtg                        >  3:32683/1‑270 (MQ=255)
                                                                                                                                                                                                                                        gACTGATTCACAAATCTGTCACTTTTCCTTACAACGAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTgggg                   <  2:407759/275‑1 (MQ=255)
                                                                                                                                                                                                                                        gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTgg                     >  2:76430/1‑273 (MQ=255)
                                                                                                                                                                                                                                         aCTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTggggg                  >  4:234095/1‑275 (MQ=255)
                                                                                                                                                                                                                                           cgATTCACAAATCTGTCACTTTTCCTTACAACAACCAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGa                 <  1:32683/273‑1 (MQ=255)
                                                                                                                                                                                                                                            gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGa                 <  4:76429/273‑1 (MQ=255)
                                                                                                                                                                                                                                                cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTaa                                                                                                                                                     <  5:435142/137‑1 (MQ=255)
                                                                                                                                                                                                                                                cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTaa                                                                                                                                                     >  6:435142/1‑137 (MQ=255)
                                                                                                                                                                                                                                                 acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                                                                                                                    <  5:1077852/121‑1 (MQ=255)
                                                                                                                                                                                                                                                 acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                                                                                                                    >  6:1077852/1‑121 (MQ=255)
                                                                                                                                                                                                                                                  caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                                                                                                                       >  5:251034/1‑69 (MQ=255)
                                                                                                                                                                                                                                                  caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                                                                                                                       <  6:251034/69‑1 (MQ=255)
                                                                                                                                                                                                                                                  caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                                                                                                                                                                                    <  6:845226/104‑1 (MQ=255)
                                                                                                                                                                                                                                                  caAATCTGTCACTGTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                                                                                                                                                                                    >  5:845226/1‑104 (MQ=255)
                                                                                                                                                                                                                                                       cTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGcc                                                                                                                                                                                                                                            >  1:30427/1‑43 (MQ=255)
                                                                                                                                                                                                                                                         gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGaa  <  2:234096/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGaa  <  2:75975/275‑1 (MQ=255)
                                                                                                                                                                                                                                                          tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                                                                                                >  2:555671/1‑164 (MQ=255)
                                                                                                                                                                                                                                                           cACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  3:30427/43‑1 (MQ=255)
                                                                                                                                                                                                                                                              ttttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAt                                                                                                            <  4:555670/164‑1 (MQ=255)
                                                                                                                                                                                                                                                               tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            >  7:435812/1‑67 (MQ=255)
                                                                                                                                                                                                                                                               tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            <  8:435812/67‑1 (MQ=255)
                                                                                                                                                                                                                                                               tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                                                                                                           >  6:486335/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                ttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGAt                                                                                      >  2:333101/1‑184 (MQ=255)
                                                                                                                                                                                                                                                                    ttACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAgg                                                                                  <  4:333100/184‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        <  5:974541/97‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        >  6:974541/1‑97 (MQ=255)
                                                                                                                                                                                                                                                                     tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                                                                                                                     <  5:486335/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                         acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaata                                                                                                                                                                                           <  6:169625/74‑1 (MQ=255)
                                                                                                                                                                                                                                                                         acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaata                                                                                                                                                                                           >  5:169625/1‑74 (MQ=255)
                                                                                                                                                                                                                                                                            aacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCTGGTCCTTTGGTCATTCa                                                                                                              >  5:852041/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                              caGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            >  7:604319/1‑52 (MQ=255)
                                                                                                                                                                                                                                                                              caGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            <  8:604319/52‑1 (MQ=255)
                                                                                                                                                                                                                                                                              caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                                                                                                                                                                          >  7:589781/1‑70 (MQ=38)
                                                                                                                                                                                                                                                                              caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                                                                                                                                                                          <  8:589781/70‑1 (MQ=38)
                                                                                                                                                                                                                                                                              caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                                            >  8:1055092/1‑132 (MQ=255)
                                                                                                                                                                                                                                                                              caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                                            <  7:1055092/132‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 ggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTg                      <  4:448973/231‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       
CCATCTGGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAA  >  NC_000913/4227276‑4227797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: