Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,227,555 A→T 12.2% intergenic (+44/‑176) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,227,5550AT12.2% 274.3 / 27.2 90intergenic (+44/‑176)metH/yjbBhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base A (35/44);  new base T (5/6);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-02

GGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAA  >  NC_000913/4227282‑4227797
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
gggAGCTGCTGGAAGGGTAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAAt                                                                                                                                                                                                                                                   <  2:142741/275‑1 (MQ=255)
  gAGGTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTTGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAGGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGa                                                                                                                                                                                                                                                 <  2:378316/275‑1 (MQ=255)
         gcgaaagagAAAAAAACACTGANNAATGANACTCAGGGAATTCTTGCCCATTAGGCCCGGGTCGTGGGTGTGGGTTTGGTACTTCAGCCACCCGGACAGCAAGTTCTACGCTGAAGCACAAATTAAGCGCGATCAGGTGGAAGGTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGcc                                                                                                                                                                                                                                            <  3:21352/267‑1 (MQ=255)
           taaGTGGGCAAAAACCCTGGCATGAAACCCAACGAAACTTTCGCCAGGTGGGCCGGTGCATCGGTTTCGGGTTGGTCCTTCAGCCACCCGGACAGCAATTACTACGCTGTAGCACAAATTCAGAGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  4:34556/274‑1 (MQ=255)
                  aaaaaCACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATTACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCCCTCCATGAGTGACCTGACTCttaa                                                                                                                                                                                                                                 >  4:351559/1‑273 (MQ=255)
                           cTGGCATGAAACTCACAGACTCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                                                                                                                        <  2:458449/275‑1 (MQ=255)
                            tGGCCTTAAACTCTCAGAGTCTTTCGCCCTGGGGCCCGTTCTATCCGTTGCGGGTTGGTCCTTCAGCCGCCCGGACAGCATGTACTCAGCTATAGCACAAATTCAGCGCTATAAAGTTGAAGATTATGCCCGACGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTAGCTGGCACCGAATCTGGGATATGACGCAGACTGATTCACAAATCTGTCACTTTTACTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                                                                                                                        <  4:31226/274‑1 (MQ=255)
                                   tGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAACTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTACAGGTATGAGCGTTACCGCAGTTGAGCGCTGGCTCGCACCTAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAACCAGTCCGCTCAATGAGTGCCCTGTCTCTTAATTAATCTGaccacct                                                                                                                                                                                                                  >  1:7906/1‑269 (MQ=255)
                                              gAATCTTTTGCCATTTGGCCCGGTGCATTGGTTTGGGGTTGGTACTTCCGCCACCCGGACAGAAAGTACTACGGTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTGCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                                                                                                                       <  2:117013/275‑1 (MQ=255)
                                               aaTCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                                                                                                                       <  2:299483/274‑1 (MQ=255)
                                                  atttgcccAGGTGGCCCCGTGAATCGGTTGCTGGTTGGTACCTGAGCCACCCGGAAATCCAATAAGTCGCTGTAGCACAAATGCAGCGAAGTCAGGTTGAAGATTTTGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAgg                                                                                                                                                                                                   <  4:384643/269‑1 (MQ=255)
                                                    ttCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGGCACTTTCCCTTACAACAAACAGGGCGCCCAATGAGGTGCCTGTCTCTTTATTAATATGAAACACTTTTCATGGAAACAGGCt                                                                                                                                                                                                 >  4:117012/1‑275 (MQ=255)
                                                                               tCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGGCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACCCTTATACTGGACACAGGCTGGAATAATCTTAGCCGGAAATACGGAt                                                                                                                                                                      >  2:482289/1‑275 (MQ=255)
                                                                                     tgggctttCAGCCACCCGGACAGCAAGTATTACGCTGTGGCTCAAATTCAGGGCGCTAAGGTTGAAGTTTAGGCCCGCCTTAAAGGTATGAGCGTTACCGAAGTTGCGCGCTGGCTGGCACCGAATCTTGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACGTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaa                                                                                                                                                                <  4:482288/269‑1 (MQ=255)
                                                                                                    ccgacaaGCAAGTCGGACGCTGTAGGAAAAATTAAGCGCGATCAGGTGGAAGATTATGGCCGCCGTAAAGGTATGAACGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTAACGTTTCCTTACAACAAACAAGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGAt                                                                                                                                                 <  4:492705/269‑1 (MQ=255)
                                                                                                                      gCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGCAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTTCTTACAACAAACACGGCGCTCAATGAGTTCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCGGGAATAATCTTAGCCGGCAAAACGAATGTCAAAAGGCACAGTCAAGATGCCAGTCAGCGTGTTTcc                                                                                                                               >  1:20923/1‑275 (MQ=255)
                                                                                                                      gCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTcc                                                                                                                               >  2:211415/1‑275 (MQ=255)
                                                                                                                                  aTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATg                                                                                                                                                                                                                                                  <  7:675729/148‑1 (MQ=255)
                                                                                                                                   ttCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGa                                                                                                                                                                                                                                                 <  8:876247/148‑1 (MQ=255)
                                                                                                                                         gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGccc                                                                                                                                                                                                                                           >  7:78799/1‑148 (MQ=255)
                                                                                                                                         gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGccc                                                                                                                                                                                                                                           <  5:980404/148‑1 (MQ=255)
                                                                                                                                            aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                <  5:196748/140‑1 (MQ=255)
                                                                                                                                            aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                >  6:196748/1‑140 (MQ=255)
                                                                                                                                            aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  8:78799/148‑1 (MQ=255)
                                                                                                                                              cAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGATTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    <  5:730134/134‑1 (MQ=255)
                                                                                                                                              cAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    >  6:730134/1‑134 (MQ=255)
                                                                                                                                                  ttggagattgatgccgccgTAAGGGTATGAGCGTTACCTAAGTTGGGCGCTGGCTGGCACCAAAACTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAgg                                                                                                   <  4:211414/262‑1 (MQ=255)
                                                                                                                                                  ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  7:743664/1‑138 (MQ=255)
                                                                                                                                                  ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  8:743664/138‑1 (MQ=255)
                                                                                                                                                   tgaagaagttGCCCGCCGAAAAGGGATGAGCGTTACCGCAGTTGAGGGATGCGTGGAACCGAATCTGGGGTATGACAGGGAATGCATCAAAAAGCTGTCACTTTTCCTTACAACAAAAAGGGCGCTCAAGGAGTGACCTGGCTCATTATTAATAAGAAACACTTATACTGGAAACAGGCTGGAATAAGCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGa                                                                                                  <  4:410841/266‑1 (MQ=255)
                                                                                                                                                            tGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaa                                                                                                                                                                 >  4:303750/1‑203 (MQ=255)
                                                                                                                                                                cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAgg                                                                                                                                                             <  2:303751/203‑1 (MQ=255)
                                                                                                                                                                cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  6:1006957/1‑124 (MQ=255)
                                                                                                                                                                cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  5:1006957/124‑1 (MQ=255)
                                                                                                                                                                        aGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                >  5:535035/1‑112 (MQ=255)
                                                                                                                                                                        aGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg                                                                                                                                                                                                                                                <  6:535035/112‑1 (MQ=255)
                                                                                                                                                                               aGCGTTACCGAAGTTTAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACt                                                                      <  2:351560/275‑1 (MQ=255)
                                                                                                                                                                                    tACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCgtg                                                                  >  2:373777/1‑274 (MQ=255)
                                                                                                                                                                                     aCCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTAATTCCATTGCTTCAGGAACATATAACGATAAGGAAAACCTGACCTCgttt                                                                 >  4:262684/1‑272 (MQ=255)
                                                                                                                                                                                                   ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCAc                                                   >  4:75974/1‑274 (MQ=255)
                                                                                                                                                                                                          gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTg                                                                                                                                                                                                                                         <  5:467346/85‑1 (MQ=255)
                                                                                                                                                                                                          gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTg                                                                                                                                                                                                                                         >  6:467346/1‑85 (MQ=255)
                                                                                                                                                                                                             ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTctct                                                                                                                                                                                                                                    >  5:928526/1‑87 (MQ=255)
                                                                                                                                                                                                             ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTctct                                                                                                                                                                                                                                    <  6:928526/87‑1 (MQ=255)
                                                                                                                                                                                                             ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  7:419172/79‑1 (MQ=39)
                                                                                                                                                                                                             ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  8:419172/1‑79 (MQ=39)
                                                                                                                                                                                                              cGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    <  6:526237/70‑1 (MQ=255)
                                                                                                                                                                                                              cGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa                                                                                                                                                                                                                                                    >  5:526237/1‑70 (MQ=255)
                                                                                                                                                                                                                        gTATGACTCGGACTGTTTCACAAATCTGTCACTTTTCCTTAAAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGAACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCctg                              <  4:373776/274‑1 (MQ=255)
                                                                                                                                                                                                                                 ggACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAANGTCAAAAGGCACAGTAAAGATGACAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGATTAAGGAANATATAAAGATAAGGAGAACATGAACGAGTGTGAAAGCGACTTCAANCNCGTTCGGACGTAGACCTGCTGGtg                        >  3:32683/1‑270 (MQ=255)
                                                                                                                                                                                                                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACGAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTgggg                   <  2:407759/275‑1 (MQ=255)
                                                                                                                                                                                                                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTgg                     >  2:76430/1‑273 (MQ=255)
                                                                                                                                                                                                                                   aCTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTggggg                  >  4:234095/1‑275 (MQ=255)
                                                                                                                                                                                                                                     cgATTCACAAATCTGTCACTTTTCCTTACAACAACCAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGa                 <  1:32683/273‑1 (MQ=255)
                                                                                                                                                                                                                                      gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGa                 <  4:76429/273‑1 (MQ=255)
                                                                                                                                                                                                                                          cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTaa                                                                                                                                                     >  6:435142/1‑137 (MQ=255)
                                                                                                                                                                                                                                          cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTaa                                                                                                                                                     <  5:435142/137‑1 (MQ=255)
                                                                                                                                                                                                                                           acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                                                                                                                    <  5:1077852/121‑1 (MQ=255)
                                                                                                                                                                                                                                           acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                                                                                                                    >  6:1077852/1‑121 (MQ=255)
                                                                                                                                                                                                                                            caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                                                                                                                       <  6:251034/69‑1 (MQ=255)
                                                                                                                                                                                                                                            caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                                                                                                                       >  5:251034/1‑69 (MQ=255)
                                                                                                                                                                                                                                            caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                                                                                                                                                                                    <  6:845226/104‑1 (MQ=255)
                                                                                                                                                                                                                                            caAATCTGTCACTGTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                                                                                                                                                                                    >  5:845226/1‑104 (MQ=255)
                                                                                                                                                                                                                                                 cTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGcc                                                                                                                                                                                                                                            >  1:30427/1‑43 (MQ=255)
                                                                                                                                                                                                                                                   gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGaa  <  2:234096/275‑1 (MQ=255)
                                                                                                                                                                                                                                                   gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGaa  <  2:75975/275‑1 (MQ=255)
                                                                                                                                                                                                                                                    tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                                                                                                >  2:555671/1‑164 (MQ=255)
                                                                                                                                                                                                                                                     cACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  3:30427/43‑1 (MQ=255)
                                                                                                                                                                                                                                                        ttttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAt                                                                                                            <  4:555670/164‑1 (MQ=255)
                                                                                                                                                                                                                                                         tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            >  7:435812/1‑67 (MQ=255)
                                                                                                                                                                                                                                                         tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            <  8:435812/67‑1 (MQ=255)
                                                                                                                                                                                                                                                         tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                                                                                                           >  6:486335/1‑148 (MQ=255)
                                                                                                                                                                                                                                                          ttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGAt                                                                                      >  2:333101/1‑184 (MQ=255)
                                                                                                                                                                                                                                                              ttACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAgg                                                                                  <  4:333100/184‑1 (MQ=255)
                                                                                                                                                                                                                                                               tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        <  5:974541/97‑1 (MQ=255)
                                                                                                                                                                                                                                                               tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        >  6:974541/1‑97 (MQ=255)
                                                                                                                                                                                                                                                               tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                                                                                                                     <  5:486335/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                   acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaata                                                                                                                                                                                           >  5:169625/1‑74 (MQ=255)
                                                                                                                                                                                                                                                                   acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaata                                                                                                                                                                                           <  6:169625/74‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCTGGTCCTTTGGTCATTCa                                                                                                              >  5:852041/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                        caGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            <  8:604319/52‑1 (MQ=255)
                                                                                                                                                                                                                                                                        caGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            >  7:604319/1‑52 (MQ=255)
                                                                                                                                                                                                                                                                        caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                                                                                                                                                                          <  8:589781/70‑1 (MQ=38)
                                                                                                                                                                                                                                                                        caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                                                                                                                                                                          >  7:589781/1‑70 (MQ=38)
                                                                                                                                                                                                                                                                        caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                                            <  7:1055092/132‑1 (MQ=255)
                                                                                                                                                                                                                                                                        caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                                            >  8:1055092/1‑132 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTg                      <  4:448973/231‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataat                                                                                                                                                                                         <  5:230960/64‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataat                                                                                                                                                                                         >  6:230960/1‑64 (MQ=255)
                                                                                                                                                                                                                                                                               cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       <  5:1129389/146‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       >  6:1129389/1‑146 (MQ=255)
                                                                                                                                                                                                                                                                               cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCa                                                                                                     >  6:324928/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                                 cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       <  6:736311/144‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       >  5:736311/1‑144 (MQ=255)
                                                                                                                                                                                                                                                                                   aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        >  7:267401/1‑77 (MQ=255)
                                                                                                                                                                                                                                                                                   aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        <  8:267401/77‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                                                                                                         <  7:564343/140‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                                                                                                         >  8:564343/1‑140 (MQ=255)
                                                                                                                                                                                                                                                                                   aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGaa                                                                                                 >  5:169043/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
GGGAGCTGCTGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAA  >  NC_000913/4227282‑4227797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: