Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,227,560 T→C 12.5% intergenic (+49/‑171) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,227,5600TC12.5% 236.4 / 21.8 88intergenic (+49/‑171)metH/yjbBhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base T (35/41);  new base C (5/6);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.19e-01

TGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAA  >  NC_000913/4227291‑4227797
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
gcgaaagagAAAAAAACACTGANNAATGANACTCAGGGAATTCTTGCCCATTAGGCCCGGGTCGTGGGTGTGGGTTTGGTACTTCAGCCACCCGGACAGCAAGTTCTACGCTGAAGCACAAATTAAGCGCGATCAGGTGGAAGGTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGcc                                                                                                                                                                                                                                            <  3:21352/267‑1 (MQ=255)
  taaGTGGGCAAAAACCCTGGCATGAAACCCAACGAAACTTTCGCCAGGTGGGCCGGTGCATCGGTTTCGGGTTGGTCCTTCAGCCACCCGGACAGCAATTACTACGCTGTAGCACAAATTCAGAGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  4:34556/274‑1 (MQ=255)
         aaaaaCACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATTACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCCCTCCATGAGTGACCTGACTCttaa                                                                                                                                                                                                                                 >  4:351559/1‑273 (MQ=255)
                  cTGGCATGAAACTCACAGACTCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                                                                                                                        <  2:458449/275‑1 (MQ=255)
                   tGGCCTTAAACTCTCAGAGTCTTTCGCCCTGGGGCCCGTTCTATCCGTTGCGGGTTGGTCCTTCAGCCGCCCGGACAGCATGTACTCAGCTATAGCACAAATTCAGCGCTATAAAGTTGAAGATTATGCCCGACGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTAGCTGGCACCGAATCTGGGATATGACGCAGACTGATTCACAAATCTGTCACTTTTACTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                                                                                                                        <  4:31226/274‑1 (MQ=255)
                          tGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAACTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTACAGGTATGAGCGTTACCGCAGTTGAGCGCTGGCTCGCACCTAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAACCAGTCCGCTCAATGAGTGCCCTGTCTCTTAATTAATCTGaccacct                                                                                                                                                                                                                  >  1:7906/1‑269 (MQ=255)
                                     gAATCTTTTGCCATTTGGCCCGGTGCATTGGTTTGGGGTTGGTACTTCCGCCACCCGGACAGAAAGTACTACGGTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTGCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                                                                                                                       <  2:117013/275‑1 (MQ=255)
                                      aaTCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                                                                                                                       <  2:299483/274‑1 (MQ=255)
                                         atttgcccAGGTGGCCCCGTGAATCGGTTGCTGGTTGGTACCTGAGCCACCCGGAAATCCAATAAGTCGCTGTAGCACAAATGCAGCGAAGTCAGGTTGAAGATTTTGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAgg                                                                                                                                                                                                   <  4:384643/269‑1 (MQ=255)
                                           ttCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGGCACTTTCCCTTACAACAAACAGGGCGCCCAATGAGGTGCCTGTCTCTTTATTAATATGAAACACTTTTCATGGAAACAGGCt                                                                                                                                                                                                 >  4:117012/1‑275 (MQ=255)
                                                                      tCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGGCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACCCTTATACTGGACACAGGCTGGAATAATCTTAGCCGGAAATACGGAt                                                                                                                                                                      >  2:482289/1‑275 (MQ=255)
                                                                            tgggctttCAGCCACCCGGACAGCAAGTATTACGCTGTGGCTCAAATTCAGGGCGCTAAGGTTGAAGTTTAGGCCCGCCTTAAAGGTATGAGCGTTACCGAAGTTGCGCGCTGGCTGGCACCGAATCTTGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACGTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaa                                                                                                                                                                <  4:482288/269‑1 (MQ=255)
                                                                                           ccgacaaGCAAGTCGGACGCTGTAGGAAAAATTAAGCGCGATCAGGTGGAAGATTATGGCCGCCGTAAAGGTATGAACGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTAACGTTTCCTTACAACAAACAAGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGAt                                                                                                                                                 <  4:492705/269‑1 (MQ=255)
                                                                                                             gCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGCAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTTCTTACAACAAACACGGCGCTCAATGAGTTCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCGGGAATAATCTTAGCCGGCAAAACGAATGTCAAAAGGCACAGTCAAGATGCCAGTCAGCGTGTTTcc                                                                                                                               >  1:20923/1‑275 (MQ=255)
                                                                                                             gCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTcc                                                                                                                               >  2:211415/1‑275 (MQ=255)
                                                                                                                                gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGccc                                                                                                                                                                                                                                           >  7:78799/1‑148 (MQ=255)
                                                                                                                                gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGccc                                                                                                                                                                                                                                           <  5:980404/148‑1 (MQ=255)
                                                                                                                                   aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  8:78799/148‑1 (MQ=255)
                                                                                                                                         ttggagattgatgccgccgTAAGGGTATGAGCGTTACCTAAGTTGGGCGCTGGCTGGCACCAAAACTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAgg                                                                                                   <  4:211414/262‑1 (MQ=255)
                                                                                                                                         ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  7:743664/1‑138 (MQ=255)
                                                                                                                                         ttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  8:743664/138‑1 (MQ=255)
                                                                                                                                          tgaagaagttGCCCGCCGAAAAGGGATGAGCGTTACCGCAGTTGAGGGATGCGTGGAACCGAATCTGGGGTATGACAGGGAATGCATCAAAAAGCTGTCACTTTTCCTTACAACAAAAAGGGCGCTCAAGGAGTGACCTGGCTCATTATTAATAAGAAACACTTATACTGGAAACAGGCTGGAATAAGCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGa                                                                                                  <  4:410841/266‑1 (MQ=255)
                                                                                                                                                   tGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaa                                                                                                                                                                 >  4:303750/1‑203 (MQ=255)
                                                                                                                                                       cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAgg                                                                                                                                                             <  2:303751/203‑1 (MQ=255)
                                                                                                                                                       cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  6:1006957/1‑124 (MQ=255)
                                                                                                                                                       cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  5:1006957/124‑1 (MQ=255)
                                                                                                                                                                      aGCGTTACCGAAGTTTAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACt                                                                      <  2:351560/275‑1 (MQ=255)
                                                                                                                                                                           tACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCgtg                                                                  >  2:373777/1‑274 (MQ=255)
                                                                                                                                                                            aCCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTAATTCCATTGCTTCAGGAACATATAACGATAAGGAAAACCTGACCTCgttt                                                                 >  4:262684/1‑272 (MQ=255)
                                                                                                                                                                                          ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCAc                                                   >  4:75974/1‑274 (MQ=255)
                                                                                                                                                                                                 gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTg                                                                                                                                                                                                                                         >  6:467346/1‑85 (MQ=255)
                                                                                                                                                                                                 gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTg                                                                                                                                                                                                                                         <  5:467346/85‑1 (MQ=255)
                                                                                                                                                                                                    ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTctct                                                                                                                                                                                                                                    >  5:928526/1‑87 (MQ=255)
                                                                                                                                                                                                    ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTctct                                                                                                                                                                                                                                    <  6:928526/87‑1 (MQ=255)
                                                                                                                                                                                                    ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            >  8:419172/1‑79 (MQ=39)
                                                                                                                                                                                                    ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                                                                                                            <  7:419172/79‑1 (MQ=39)
                                                                                                                                                                                                               gTATGACTCGGACTGTTTCACAAATCTGTCACTTTTCCTTAAAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGAACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCctg                              <  4:373776/274‑1 (MQ=255)
                                                                                                                                                                                                                        ggACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAANGTCAAAAGGCACAGTAAAGATGACAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGATTAAGGAANATATAAAGATAAGGAGAACATGAACGAGTGTGAAAGCGACTTCAANCNCGTTCGGACGTAGACCTGCTGGtg                        >  3:32683/1‑270 (MQ=255)
                                                                                                                                                                                                                         gACTGATTCACAAATCTGTCACTTTTCCTTACAACGAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTgggg                   <  2:407759/275‑1 (MQ=255)
                                                                                                                                                                                                                         gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTgg                     >  2:76430/1‑273 (MQ=255)
                                                                                                                                                                                                                          aCTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTggggg                  >  4:234095/1‑275 (MQ=255)
                                                                                                                                                                                                                            cgATTCACAAATCTGTCACTTTTCCTTACAACAACCAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGa                 <  1:32683/273‑1 (MQ=255)
                                                                                                                                                                                                                             gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGa                 <  4:76429/273‑1 (MQ=255)
                                                                                                                                                                                                                                 cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTaa                                                                                                                                                     >  6:435142/1‑137 (MQ=255)
                                                                                                                                                                                                                                 cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTaa                                                                                                                                                     <  5:435142/137‑1 (MQ=255)
                                                                                                                                                                                                                                  acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                                                                                                                    <  5:1077852/121‑1 (MQ=255)
                                                                                                                                                                                                                                  acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                                                                                                                    >  6:1077852/1‑121 (MQ=255)
                                                                                                                                                                                                                                   caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                                                                                                                       <  6:251034/69‑1 (MQ=255)
                                                                                                                                                                                                                                   caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                                                                                                                       >  5:251034/1‑69 (MQ=255)
                                                                                                                                                                                                                                   caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                                                                                                                                                                                    <  6:845226/104‑1 (MQ=255)
                                                                                                                                                                                                                                   caAATCTGTCACTGTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                                                                                                                                                                                    >  5:845226/1‑104 (MQ=255)
                                                                                                                                                                                                                                        cTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGcc                                                                                                                                                                                                                                            >  1:30427/1‑43 (MQ=255)
                                                                                                                                                                                                                                          gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGaa  <  2:234096/275‑1 (MQ=255)
                                                                                                                                                                                                                                          gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGaa  <  2:75975/275‑1 (MQ=255)
                                                                                                                                                                                                                                           tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                                                                                                >  2:555671/1‑164 (MQ=255)
                                                                                                                                                                                                                                            cACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGt                                                                                                                                                                                                                                        <  3:30427/43‑1 (MQ=255)
                                                                                                                                                                                                                                               ttttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAt                                                                                                            <  4:555670/164‑1 (MQ=255)
                                                                                                                                                                                                                                                tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            <  8:435812/67‑1 (MQ=255)
                                                                                                                                                                                                                                                tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            >  7:435812/1‑67 (MQ=255)
                                                                                                                                                                                                                                                tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                                                                                                           >  6:486335/1‑148 (MQ=255)
                                                                                                                                                                                                                                                 ttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGAt                                                                                      >  2:333101/1‑184 (MQ=255)
                                                                                                                                                                                                                                                     ttACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAgg                                                                                  <  4:333100/184‑1 (MQ=255)
                                                                                                                                                                                                                                                      tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        <  5:974541/97‑1 (MQ=255)
                                                                                                                                                                                                                                                      tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        >  6:974541/1‑97 (MQ=255)
                                                                                                                                                                                                                                                      tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                                                                                                                     <  5:486335/148‑1 (MQ=255)
                                                                                                                                                                                                                                                          acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaata                                                                                                                                                                                           <  6:169625/74‑1 (MQ=255)
                                                                                                                                                                                                                                                          acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaata                                                                                                                                                                                           >  5:169625/1‑74 (MQ=255)
                                                                                                                                                                                                                                                             aacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCTGGTCCTTTGGTCATTCa                                                                                                              >  5:852041/1‑148 (MQ=255)
                                                                                                                                                                                                                                                               caGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            >  7:604319/1‑52 (MQ=255)
                                                                                                                                                                                                                                                               caGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                                                                                                                                                                            <  8:604319/52‑1 (MQ=255)
                                                                                                                                                                                                                                                               caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                                                                                                                                                                          >  7:589781/1‑70 (MQ=38)
                                                                                                                                                                                                                                                               caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                                                                                                                                                                          <  8:589781/70‑1 (MQ=38)
                                                                                                                                                                                                                                                               caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                                            <  7:1055092/132‑1 (MQ=255)
                                                                                                                                                                                                                                                               caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGt                                                                                                                            >  8:1055092/1‑132 (MQ=255)
                                                                                                                                                                                                                                                                  ggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTg                      <  4:448973/231‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataat                                                                                                                                                                                         >  6:230960/1‑64 (MQ=255)
                                                                                                                                                                                                                                                                      cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataat                                                                                                                                                                                         <  5:230960/64‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       <  5:1129389/146‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       >  6:1129389/1‑146 (MQ=255)
                                                                                                                                                                                                                                                                      cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCa                                                                                                     >  6:324928/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                        cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       >  5:736311/1‑144 (MQ=255)
                                                                                                                                                                                                                                                                        cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt                                                                                                       <  6:736311/144‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        >  7:267401/1‑77 (MQ=255)
                                                                                                                                                                                                                                                                          aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                                                                                                                        <  8:267401/77‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                                                                                                         >  8:564343/1‑140 (MQ=255)
                                                                                                                                                                                                                                                                          aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                                                                                                         <  7:564343/140‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGaa                                                                                                 >  5:169043/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                             aGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACat                                                                                              <  6:169043/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                               tGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACatat                                                                                            >  8:700395/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
TGGAAGTGGAAAAACACACTGGCATGAAACTCACAGAATCTTTCGCCATGTGGCCCGGTGCATCGGTTTCGGGTTGGTACTTCAGCCACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGAGAACCTGAACTCGTGTTAACGCTGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAA  >  NC_000913/4227291‑4227797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: