Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,235,470 C→A 6.5% intergenic (+63/‑436) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,4700CA 6.5% 204.3 / 5.5 63intergenic (+63/‑436)pgi/yjbEglucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base C (21/37);  new base A (2/2);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-02

CCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTT  >  NC_000913/4235200‑4235713
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
ccTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGGTCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAACCAGCTGACGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAACTCAGCAGCCACGATATCCCGACCAACGGTCTGATTAACCGCTATAAACCGTGGCGCGGTTAATCCTCGTCGCTATGTCGCCCGGAAAAGGCGTTCACGCCGCATTCGGCAACCGTCGCCTAATGCGA‑cc                                                                                                                                                                                                                                                 >  4:162896/1‑274 (MQ=255)
    ggTGCGTTGATTTCGCGGTAGGGGACAAAAACCTTGACTCAGGGGGTGATCCTGAACATCTTCACCTCCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCGGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGt                                                                                                                                                                                                                                             <  4:222021/275‑1 (MQ=255)
                                aaaTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACGGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATg                                                                                                                                                                                                                 >  2:107703/1‑275 (MQ=255)
                                aaaTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGCATAAGGCGTTCACGCCGAATCCGCCAACCGATGCCTGATGCTA‑CGCGGTCGCGTCGTATCAGGCCTCCAGGTCGAt                                                                                                                                                                                                                  >  4:50944/1‑274 (MQ=255)
                                  aTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                                                                                                                                                                               <  2:142909/275‑1 (MQ=255)
                                  aTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCGTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGGCGATATGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                                                                                                                                                                               <  2:100443/275‑1 (MQ=255)
                                   tCTTTAATCAGGGCTTGATCCTGAACGTCTTCACCTTCGACCAGTGGGGCGGTGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAAACAGCAGCCACGATAGCTCGACCAATGTTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                                                                                                                                                                               <  2:86563/274‑1 (MQ=255)
                                            aGGGGGTGAGCCTGAACACATTCACCTTGGACCAGGGGGGCGTGGAACTGGGTAAACATCGTGCGAACCGTATTCTGCAAGGGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCCCGACCAATGGTCTGATGAACCGCTATAACGCGTGGCGCGGTTAATCATCGTCGATAGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                                                                                                                                                                                     <  4:270102/275‑1 (MQ=255)
                                            aGGGCGTTATCCTGAACATCTTCACCTTCGACAAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                                                                                                                                                                                     <  2:127308/275‑1 (MQ=255)
                                                       cgtaaCATCTTAACCTTCGACCAGTGGGGCGTGGAACTGGGTAAAAAGCTGGCGAACCTTATTATGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGTTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:1296/272‑1 (MQ=255)
                                                       cTGAACATCTTCACCTTCGTCTAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGTGGATAAAGAAATCAGCAGCCACGATAGCTCGACTAATGGTCTGATTAACCGCTATAAAGCGTGGCGAGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:535208/275‑1 (MQ=255)
                                                       cTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  4:136365/275‑1 (MQ=255)
                                                       cTGAACATCGTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCGATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:359051/275‑1 (MQ=255)
                                                       atGAACATATTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAGCAGCTGGCGAACCGTATGCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAACCATGGCCGATATGTAGGACGGATAAGGCGTTCACGCCGCATACGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:162897/274‑1 (MQ=39)
                                                        tGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACGACTGGCGAACCGTATTCTGCCAGAGCTGAAAGATAATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:445266/274‑1 (MQ=255)
                                                        tGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGGGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          >  2:92969/1‑274 (MQ=255)
                                                            cATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                                                                                                                                                                                      <  2:193147/274‑1 (MQ=255)
                                                              tCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCAGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAg                                                                                                                                                                                                                                <  2:485925/230‑1 (MQ=255)
                                                               cTTCCCCTTCGGCCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa                                                                                                                                                                                  <  1:41520/275‑1 (MQ=255)
                                                               cTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAACTCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGGGACCGCGG‑CGCGTCTTATCAGGCCTCCAGGTCGATGCCGGTATGTACCTCGTATGCGGGAATTCATa                                                                                                                                                                                  >  4:1296/1‑275 (MQ=255)
                                                                                    gttGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa                                                                                                                                                             <  2:24245/273‑1 (MQ=255)
                                                                                    cGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa                                                                                                                                                             >  2:204523/1‑275 (MQ=255)
                                                                                                   aCAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTAGCGGCTTATCAGGCCTNCAGGACGATGCCGATATGTACAGAGTATTACGCAATTAATACAGTACACGAGTGAATAAATACACTTAATAACaaaa                                                                                                                                              >  3:113720/1‑273 (MQ=255)
                                                                                                        tGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCt                                                                                                                                                                                                                                      >  4:126884/1‑182 (MQ=255)
                                                                                                        tGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGAc                                                                                                                                         >  2:214229/1‑275 (MQ=255)
                                                                                                            gAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATc                                                                                                                                                                                                                                  <  2:126885/182‑1 (MQ=255)
                                                                                                               ccGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                                                                                                                                                                                         >  2:342971/1‑204 (MQ=255)
                                                                                                                   aTTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                                                                                                                                                                                     <  4:342970/204‑1 (MQ=255)
                                                                                                                            gagCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑cg                                                                                                                                                                                                                                                 >  4:197231/1‑151 (MQ=255)
                                                                                                                                tGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGt                                                                                                                                                                                                                                             <  2:197232/151‑1 (MQ=255)
                                                                                                                                   aaGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGAt                                                                                                               <  4:92968/274‑1 (MQ=255)
                                                                                                                                            aaGAAATCAGCGGCCACGAGTGCTCGACCTATGGTCTGATTAACCGCTATAAAGCGTGGCTGGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcccc                                                                                                       <  4:204522/273‑1 (MQ=255)
                                                                                                                                               aaaTCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCTGCAATTAATACATAGCACGATTGATTAAATAACCTTAAGAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTc                                                                                                  <  1:113720/275‑1 (MQ=255)
                                                                                                                                                     gcagcCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                           <  5:204687/148‑1 (MQ=255)
                                                                                                                                                              aTAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACgg                                                                                     <  4:107702/273‑1 (MQ=255)
                                                                                                                                                                                  aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                                                                                    >  8:1115850/1‑142 (MQ=255)
                                                                                                                                                                                  aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGACGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACaa                                                                                                                                                                                                    <  7:1115850/142‑2 (MQ=255)
                                                                                                                                                                                    tAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGc                                                                                                                                                                                                                              >  7:37133/1‑114 (MQ=255)
                                                                                                                                                                                    tAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGc                                                                                                                                                                                                                              <  8:37133/114‑1 (MQ=255)
                                                                                                                                                                                                     ggCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                                                                                                                                                                              >  2:552124/1‑129 (MQ=255)
                                                                                                                                                                                                      gcgcGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                                                                                                                                                                                            >  8:1021192/1‑114 (MQ=255)
                                                                                                                                                                                                      gcgcGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                                                                                                                                                                                            <  7:1021192/114‑1 (MQ=255)
                                                                                                                                                                                                       cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                                                                      <  5:1000266/119‑1 (MQ=255)
                                                                                                                                                                                                       cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                                                                      >  6:1000266/1‑119 (MQ=255)
                                                                                                                                                                                                         cgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  4:552123/129‑1 (MQ=255)
                                                                                                                                                                                                             tAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                                                                                                                                                                                    >  5:183304/1‑68 (MQ=255)
                                                                                                                                                                                                             tAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                                                                                                                                                                                    <  6:183304/68‑1 (MQ=255)
                                                                                                                                                                                                             tAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑cg                                                                                                                                                                                                                                                 >  5:918039/1‑70 (MQ=255)
                                                                                                                                                                                                             tAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑cg                                                                                                                                                                                                                                                 <  6:918039/70‑1 (MQ=255)
                                                                                                                                                                                                                         atatGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                                                                                                                                                                              >  4:457272/1‑109 (MQ=255)
                                                                                                                                                                                                                           agtgaGGCCGGATAAGGGGGTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACg                      <  4:214228/271‑1 (MQ=255)
                                                                                                                                                                                                                             gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:457273/109‑1 (MQ=255)
                                                                                                                                                                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                                                                                 >  2:556136/1‑114 (MQ=255)
                                                                                                                                                                                                                                     gATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg                                                                                                                                                                             <  4:556135/114‑1 (MQ=255)
                                                                                                                                                                                                                                          ggCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                                                                                                                                                                                   <  6:126864/103‑1 (MQ=255)
                                                                                                                                                                                                                                          ggCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                                                                                                                                                                                   >  5:126864/1‑103 (MQ=255)
                                                                                                                                                                                                                                            cGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGA‑CGCGACCGCGTCGCATCAg                                                                                                                                                                                                                                <  7:185247/56‑1 (MQ=11)
                                                                                                                                                                                                                                            cGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGA‑CGCGACCGCGTCGCATCAg                                                                                                                                                                                                                                >  8:185247/1‑56 (MQ=2)
                                                                                                                                                                                                                                              ttCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTAt                                                                                                                                                                                                                                   >  6:830067/1‑51 (MQ=255)
                                                                                                                                                                                                                                              ttCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTAt                                                                                                                                                                                                                                   <  5:830067/51‑1 (MQ=255)
                                                                                                                                                                                                                                                cACGCCGCAGCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGttt  <  2:50945/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gtaggCCTGATAAGA‑CGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTta                                                                                                                                                       <  7:1040467/103‑1 (MQ=21)
                                                                                                                                                                                                                                                                  gtaggCCTGATAAGA‑CGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTta                                                                                                                                                       >  8:1040467/5‑107 (MQ=21)
                                                                                                                                                                                                                                                                              cGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc                                                                                                                                                          >  7:550911/1‑92 (MQ=255)
                                                                                                                                                                                                                                                                              cGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc                                                                                                                                                          <  8:550911/92‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
CCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTT  >  NC_000913/4235200‑4235713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑