| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,235,485 | T→G | 11.7% | intergenic (+78/‑421) | pgi → / → yjbE | glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,235,485 | 0 | T | G | 11.7% | 200.6 / 18.7 | 65 | intergenic (+78/‑421) | pgi/yjbE | glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base T (20/37); new base G (4/4); total (24/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-03 | |||||||||||
AAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTT > NC_000913/4235232‑4235713 | aaaTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACGGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATg > 2:107703/1‑275 (MQ=255)aaaTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGCATAAGGCGTTCACGCCGAATCCGCCAACCGATGCCTGATGCTA‑CGCGGTCGCGTCGTATCAGGCCTCCAGGTCGAt > 4:50944/1‑274 (MQ=255) aTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc < 2:142909/275‑1 (MQ=255) aTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCGTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGGCGATATGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc < 2:100443/275‑1 (MQ=255) tCTTTAATCAGGGCTTGATCCTGAACGTCTTCACCTTCGACCAGTGGGGCGGTGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAAACAGCAGCCACGATAGCTCGACCAATGTTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc < 2:86563/274‑1 (MQ=255) aGGGGGTGAGCCTGAACACATTCACCTTGGACCAGGGGGGCGTGGAACTGGGTAAACATCGTGCGAACCGTATTCTGCAAGGGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCCCGACCAATGGTCTGATGAACCGCTATAACGCGTGGCGCGGTTAATCATCGTCGATAGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 4:270102/275‑1 (MQ=255) aGGGCGTTATCCTGAACATCTTCACCTTCGACAAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 2:127308/275‑1 (MQ=255) cgtaaCATCTTAACCTTCGACCAGTGGGGCGTGGAACTGGGTAAAAAGCTGGCGAACCTTATTATGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGTTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 2:1296/272‑1 (MQ=255) cTGAACATCTTCACCTTCGTCTAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGTGGATAAAGAAATCAGCAGCCACGATAGCTCGACTAATGGTCTGATTAACCGCTATAAAGCGTGGCGAGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 2:535208/275‑1 (MQ=255) cTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 4:136365/275‑1 (MQ=255) cTGAACATCGTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCGATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 2:359051/275‑1 (MQ=255) atGAACATATTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAGCAGCTGGCGAACCGTATGCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAACCATGGCCGATATGTAGGACGGATAAGGCGTTCACGCCGCATACGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 2:162897/274‑1 (MQ=39) tGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACGACTGGCGAACCGTATTCTGCCAGAGCTGAAAGATAATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 2:445266/274‑1 (MQ=255) tGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGGGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc > 2:92969/1‑274 (MQ=255) cATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt < 2:193147/274‑1 (MQ=255) tCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCAGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAg < 2:485925/230‑1 (MQ=255) cTTCCCCTTCGGCCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa < 1:41520/275‑1 (MQ=255) cTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAACTCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGGGACCGCGG‑CGCGTCTTATCAGGCCTCCAGGTCGATGCCGGTATGTACCTCGTATGCGGGAATTCATa > 4:1296/1‑275 (MQ=255) gttGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa < 2:24245/273‑1 (MQ=255) cGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa > 2:204523/1‑275 (MQ=255) aCAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTAGCGGCTTATCAGGCCTNCAGGACGATGCCGATATGTACAGAGTATTACGCAATTAATACAGTACACGAGTGAATAAATACACTTAATAACaaaa > 3:113720/1‑273 (MQ=255) tGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCt > 4:126884/1‑182 (MQ=255) tGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGAc > 2:214229/1‑275 (MQ=255) gAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATc < 2:126885/182‑1 (MQ=255) ccGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg > 2:342971/1‑204 (MQ=255) aTTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 4:342970/204‑1 (MQ=255) aaGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGAt < 4:92968/274‑1 (MQ=255) aaGAAATCAGCGGCCACGAGTGCTCGACCTATGGTCTGATTAACCGCTATAAAGCGTGGCTGGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcccc < 4:204522/273‑1 (MQ=255) aaaTCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCTGCAATTAATACATAGCACGATTGATTAAATAACCTTAAGAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTc < 1:113720/275‑1 (MQ=255) gcagcCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTa < 5:204687/148‑1 (MQ=255) aTAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACgg < 4:107702/273‑1 (MQ=255) aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt > 8:1115850/1‑142 (MQ=255) aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGACGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACaa < 7:1115850/142‑2 (MQ=255) tAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGc > 7:37133/1‑114 (MQ=255) tAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGc < 8:37133/114‑1 (MQ=255) ggCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt > 2:552124/1‑129 (MQ=255) gcgcGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat > 8:1021192/1‑114 (MQ=255) gcgcGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat < 7:1021192/114‑1 (MQ=255) cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc < 5:1000266/119‑1 (MQ=255) cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc > 6:1000266/1‑119 (MQ=255) cgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 4:552123/129‑1 (MQ=255) atatGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt > 4:457272/1‑109 (MQ=255) agtgaGGCCGGATAAGGGGGTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACg < 4:214228/271‑1 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 2:457273/109‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc > 2:556136/1‑114 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg < 4:556135/114‑1 (MQ=255) ggCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt < 6:126864/103‑1 (MQ=255) ggCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt > 5:126864/1‑103 (MQ=255) cGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGA‑CGCGACCGCGTCGCATCAg > 8:185247/1‑56 (MQ=2) cGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGA‑CGCGACCGCGTCGCATCAg < 7:185247/56‑1 (MQ=11) ttCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTAt < 5:830067/51‑1 (MQ=255) ttCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTAt > 6:830067/1‑51 (MQ=255) cACGCCGCAGCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGttt < 2:50945/274‑1 (MQ=255) gtaggCCTGATAAGA‑CGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTta > 8:1040467/5‑107 (MQ=21) gtaggCCTGATAAGA‑CGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTta < 7:1040467/103‑1 (MQ=21) ataagA‑CGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc < 7:232393/91‑1 (MQ=37) aagA‑CGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattga > 5:548857/3‑83 (MQ=37) aagA‑CGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattga < 6:548857/81‑1 (MQ=38) cGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc < 8:550911/92‑1 (MQ=255) cGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc > 7:550911/1‑92 (MQ=255) cgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg > 6:886627/1‑104 (MQ=255) cgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg < 5:886627/104‑1 (MQ=255) tCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg < 6:808639/102‑1 (MQ=255) tCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg > 5:808639/1‑102 (MQ=255) cTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTa > 6:761408/1‑138 (MQ=255) cTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTa < 5:761408/138‑1 (MQ=255) | AAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA‑CGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTT > NC_000913/4235232‑4235713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |