Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,235,486 T→C 10.8% intergenic (+79/‑420) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,4860TC10.8% 203.9 / 12.7 65intergenic (+79/‑420)pgi/yjbEglucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base T (21/37);  new base C (3/4);  total (24/41)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-04

AAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTT  >  NC_000913/4235232‑4235713
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   
aaaTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACGGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATg                                                                                                                                                                                                                 >  2:107703/1‑275 (MQ=255)
aaaTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGCATAAGGCGTTCACGCCGAATCCGCCAACCGATGCCTGATGCTACGCGGTCGCGTCGTATCAGGCCTCCAGGTCGAt                                                                                                                                                                                                                  >  4:50944/1‑274 (MQ=255)
  aTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                                                                                                                                                                               <  2:142909/275‑1 (MQ=255)
  aTCTTTACTCAGGGCGTGATCCTGAACATCTTCACCGTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGGCGATATGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                                                                                                                                                                               <  2:100443/275‑1 (MQ=255)
   tCTTTAATCAGGGCTTGATCCTGAACGTCTTCACCTTCGACCAGTGGGGCGGTGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAAACAGCAGCCACGATAGCTCGACCAATGTTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                                                                                                                                                                                               <  2:86563/274‑1 (MQ=255)
            aGGGGGTGAGCCTGAACACATTCACCTTGGACCAGGGGGGCGTGGAACTGGGTAAACATCGTGCGAACCGTATTCTGCAAGGGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCCCGACCAATGGTCTGATGAACCGCTATAACGCGTGGCGCGGTTAATCATCGTCGATAGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                                                                                                                                                                                     <  4:270102/275‑1 (MQ=255)
            aGGGCGTTATCCTGAACATCTTCACCTTCGACAAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                                                                                                                                                                                     <  2:127308/275‑1 (MQ=255)
                       cgtaaCATCTTAACCTTCGACCAGTGGGGCGTGGAACTGGGTAAAAAGCTGGCGAACCTTATTATGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGTTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:1296/272‑1 (MQ=255)
                       cTGAACATCTTCACCTTCGTCTAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGTGGATAAAGAAATCAGCAGCCACGATAGCTCGACTAATGGTCTGATTAACCGCTATAAAGCGTGGCGAGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:535208/275‑1 (MQ=255)
                       cTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  4:136365/275‑1 (MQ=255)
                       cTGAACATCGTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCGATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:359051/275‑1 (MQ=255)
                       atGAACATATTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAGCAGCTGGCGAACCGTATGCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACAAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAACCATGGCCGATATGTAGGACGGATAAGGCGTTCACGCCGCATACGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:162897/274‑1 (MQ=39)
                        tGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACGACTGGCGAACCGTATTCTGCCAGAGCTGAAAGATAATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:445266/274‑1 (MQ=255)
                        tGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGGGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          >  2:92969/1‑274 (MQ=255)
                            cATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                                                                                                                                                                                      <  2:193147/274‑1 (MQ=255)
                              tCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCAGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                                                                                                                                                                                                <  2:485925/230‑1 (MQ=255)
                               cTTCCCCTTCGGCCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa                                                                                                                                                                                  <  1:41520/275‑1 (MQ=255)
                               cTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAACTCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGGGACCGCGGCGCGTCTTATCAGGCCTCCAGGTCGATGCCGGTATGTACCTCGTATGCGGGAATTCATa                                                                                                                                                                                  >  4:1296/1‑275 (MQ=255)
                                                    gttGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa                                                                                                                                                             <  2:24245/273‑1 (MQ=255)
                                                    cGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa                                                                                                                                                             >  2:204523/1‑275 (MQ=255)
                                                                   aCAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTAGCGGCTTATCAGGCCTNCAGGACGATGCCGATATGTACAGAGTATTACGCAATTAATACAGTACACGAGTGAATAAATACACTTAATAACaaaa                                                                                                                                              >  3:113720/1‑273 (MQ=255)
                                                                        tGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGAc                                                                                                                                         >  2:214229/1‑275 (MQ=255)
                                                                            gAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                                                                                  <  2:126885/182‑1 (MQ=255)
                                                                               ccGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                                                                                                                                                                                         >  2:342971/1‑204 (MQ=255)
                                                                                   aTTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                                                                                                                                                                                                     <  4:342970/204‑1 (MQ=255)
                                                                                                   aaGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGAt                                                                                                               <  4:92968/274‑1 (MQ=255)
                                                                                                            aaGAAATCAGCGGCCACGAGTGCTCGACCTATGGTCTGATTAACCGCTATAAAGCGTGGCTGGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcccc                                                                                                       <  4:204522/273‑1 (MQ=255)
                                                                                                               aaaTCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCTGCAATTAATACATAGCACGATTGATTAAATAACCTTAAGAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTc                                                                                                  <  1:113720/275‑1 (MQ=255)
                                                                                                                     gcagcCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                           <  5:204687/148‑1 (MQ=255)
                                                                                                                              aTAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACgg                                                                                     <  4:107702/273‑1 (MQ=255)
                                                                                                                                                  aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                                                                                                                                                                                                    >  8:1115850/1‑142 (MQ=255)
                                                                                                                                                  aTTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGACGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACaa                                                                                                                                                                                                    <  7:1115850/142‑2 (MQ=255)
                                                                                                                                                    tAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                                                                                                                                                                              >  7:37133/1‑114 (MQ=255)
                                                                                                                                                    tAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                                                                                                                                                                              <  8:37133/114‑1 (MQ=255)
                                                                                                                                                                     ggCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                                                                                                                                                                              >  2:552124/1‑129 (MQ=255)
                                                                                                                                                                      gcgcGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                                                                                                                                                                                            >  8:1021192/1‑114 (MQ=255)
                                                                                                                                                                      gcgcGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                                                                                                                                                                                            <  7:1021192/114‑1 (MQ=255)
                                                                                                                                                                       cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                                                                      <  5:1000266/119‑1 (MQ=255)
                                                                                                                                                                       cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                                                                      >  6:1000266/1‑119 (MQ=255)
                                                                                                                                                                         cgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  4:552123/129‑1 (MQ=255)
                                                                                                                                                                                         atatGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                                                                                                                                                                              >  4:457272/1‑109 (MQ=255)
                                                                                                                                                                                           agtgaGGCCGGATAAGGGGGTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACg                      <  4:214228/271‑1 (MQ=255)
                                                                                                                                                                                             gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                                                                          <  2:457273/109‑1 (MQ=255)
                                                                                                                                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                                                                                                                                                                                 >  2:556136/1‑114 (MQ=255)
                                                                                                                                                                                                     gATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg                                                                                                                                                                             <  4:556135/114‑1 (MQ=255)
                                                                                                                                                                                                          ggCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                                                                                                                                                                                   <  6:126864/103‑1 (MQ=255)
                                                                                                                                                                                                          ggCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                                                                                                                                                                                   >  5:126864/1‑103 (MQ=255)
                                                                                                                                                                                                            cGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAg                                                                                                                                                                                                                                >  8:185247/1‑56 (MQ=2)
                                                                                                                                                                                                            cGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAg                                                                                                                                                                                                                                <  7:185247/56‑1 (MQ=11)
                                                                                                                                                                                                              ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                                                                                                                                                                                                   <  5:830067/51‑1 (MQ=255)
                                                                                                                                                                                                              ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                                                                                                                                                                                                   >  6:830067/1‑51 (MQ=255)
                                                                                                                                                                                                                cACGCCGCAGCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGttt  <  2:50945/274‑1 (MQ=255)
                                                                                                                                                                                                                                  gtaggCCTGATAAGACGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTta                                                                                                                                                       >  8:1040467/5‑107 (MQ=21)
                                                                                                                                                                                                                                  gtaggCCTGATAAGACGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTta                                                                                                                                                       <  7:1040467/103‑1 (MQ=21)
                                                                                                                                                                                                                                           ataagACGCGACCGCGTCGCATCAGGCATACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc                                                                                                                                                          <  7:232393/91‑1 (MQ=37)
                                                                                                                                                                                                                                             aagACGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattga                                                                                                                                                                    >  5:548857/3‑83 (MQ=37)
                                                                                                                                                                                                                                             aagACGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattga                                                                                                                                                                    <  6:548857/81‑1 (MQ=38)
                                                                                                                                                                                                                                              cGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc                                                                                                                                                          <  8:550911/92‑1 (MQ=255)
                                                                                                                                                                                                                                              cGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAAcc                                                                                                                                                          >  7:550911/1‑92 (MQ=255)
                                                                                                                                                                                                                                                          cgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg                                                                                                                                   >  6:886627/1‑104 (MQ=255)
                                                                                                                                                                                                                                                          cgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg                                                                                                                                   <  5:886627/104‑1 (MQ=255)
                                                                                                                                                                                                                                                            tCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg                                                                                                                                   <  6:808639/102‑1 (MQ=255)
                                                                                                                                                                                                                                                            tCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATg                                                                                                                                   >  5:808639/1‑102 (MQ=255)
                                                                                                                                                                                                                                                             cTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTa                                                                                              >  6:761408/1‑138 (MQ=255)
                                                                                                                                                                                                                                                             cTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTa                                                                                              <  5:761408/138‑1 (MQ=255)
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AAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTT  >  NC_000913/4235232‑4235713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: