Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,262,250 | G→C | 10.2% | G194G (GGG→GGC) | dusA → | tRNA‑dihydrouridine synthase A |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,262,250 | 0 | G | C | 10.2% | 547.4 / 41.6 | 167 | G194G (GGG→GGC) | dusA | tRNA‑dihydrouridine synthase A |
Reads supporting (aligned to +/- strand): ref base G (79/70); new base C (9/8); total (89/78) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-01 |
Only 100 of 168 total aligned reads displayed.
GGATATGATGAGATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCG > NC_000913/4261978‑4262520 | aTCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGTGTCTTGGCATCCACGGCCAGGACAGCTATGAATTGCTATGCGAT‑TTCATCAATACCGTTTCCGGCCAAGGCGAGTGTGAGATGTTCATCATCCACCCACGTAAAGCCTGGCTTTCGGTGTTAATCCCgaacg > 4:320213/1‑272 (MQ=255) aTCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaag > 2:387851/1‑274 (MQ=255) cAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGAAAATGCGAGTGTGAGATGTGCATCATCCACGCACGTAAAGCATGGCTTTCGGGGTTAATCCCgaaagaa > 1:925/1‑274 (MQ=255) cAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGGGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaagaa < 2:69304/274‑1 (MQ=255) aaCCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaagaaa > 2:458279/1‑274 (MQ=255) aCCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGATTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGTTTAAGCCCGaaaaaaaa > 4:441424/1‑274 (MQ=255) tcGGCGTTTTTGGTGCGTGCCTGATGGGTAATGCGCCGTTGTTTGCCGACTACGTGAACGCGATGCGCGTTGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATGGGCATCAACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCAGCAAAGGCGAGTGTTAGATGTTCATCATCCACGAACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTAt < 1:79432/274‑1 (MQ=255) cATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGGTAAGCCCGAACGAACACCGGGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTAGCaat > 2:461155/1‑274 (MQ=255) cATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTAGTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGCGATGTTCATCATCCTCGCACGTAAAGACGAGCTTTCGGGGTTAAGCCCGAAAGAAACCCGTGAACTCCCGCCGCCCGATTATCAGCGT‑GTGTATCaa > 4:260333/1‑274 (MQ=255) agtggCTGATTGCCGATTATGTGAATGGAATGCGCGATTTGGGTGCGGCTCCGGTGACGGTGAAACGGCGAATTGCACACGATGACCAGGACACGAATGAATTTTCCTTCTAT‑TCCATCATAACCGTATCCGCCAAAGGCGATTGTGAGATGTTCATCAGCCACGCACTTACAGCCAGGCGTTCGTGGTTAAGCCCGAAAGAAAGCCGTGAGACCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATg < 4:505915/271‑1 (MQ=255) cTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGAt > 2:230633/1‑275 (MQ=255) aCTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGCT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGTAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggctg > 2:505916/1‑271 (MQ=255) gCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTa > 2:344613/1‑273 (MQ=255) tGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGaa < 2:371350/275‑1 (MQ=255) gtCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTa > 6:653262/1‑148 (MQ=255) cGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAg > 5:242414/1‑148 (MQ=255) tcggtgagggttaAAAAGCGGTTTTGCGTCGACGGAGAGGACAGGTATGAATTTTTCGGGGAG‑TTCTGTAATACGGTTACGGGCAAGGGGGGGGGTGAGATGTTCATCATCGACGGTCGTAAAGTCTGGCTTTCGGGGTTAAGCCCGGAAGAAAACCGTGAAATCCCGCAGCTCGATTATCCCCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 4:79306/273‑1 (MQ=255) ccGGTGACGGGGAAAACGGGTATTGGAATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATAAATCCCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGAACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc < 4:282568/274‑1 (MQ=255) gggtgcggtgaAAGCGCGTTATGGAATCGACAACCAGAACGGCTATGAATTTCTCTGCGGT‑TTCAACAATGCCGTTACAGGCAAAGGCGGGTGGGAGATGTTCATCAGCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCACCGCTGGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCaa < 4:448198/269‑1 (MQ=255) acggtgaAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaagaaa < 7:1045122/148‑1 (MQ=255) gaAAACGCTGATTGGCATCGTCGACCAGAAAAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGACAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATatggat < 2:133648/274‑1 (MQ=255) gaAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaa > 7:721477/1‑138 (MQ=255) aaaaCGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCg < 4:399684/146‑1 (MQ=255) cgTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGcc > 2:202968/1‑243 (MQ=255) aTTGGCATTCGCGACCAGGAATGCTATTGATTTCTCTGCGAT‑TTCAACAAAACCGTTTCCGGAAAAGGGGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGt < 4:112653/275‑1 (MQ=255) ttGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAg < 4:202967/243‑1 (MQ=255) ttGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCGGGAAGAGGCAAAAGCCACCCTGCACAATATGGATGGCGTGGTGGTc > 4:213730/1‑275 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaa > 5:355616/1‑122 (MQ=255) aTCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaa < 6:355616/122‑1 (MQ=255) aTCGACGACAAGGCCAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGgcg < 2:445838/275‑1 (MQ=255) ggacgacAAGGACATGAATGAATTTGTC‑GGGATGTGCATAAGAACCATTTCCGGCAAAGGCGGGTGGGAGATGTTCAGGAGCCACGAACGAAAACCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTGTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGcgcg < 4:345934/274‑1 (MQ=255) cgacgaCCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGcgcg < 2:255872/275‑1 (MQ=255) ggACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCgaaaga > 7:206456/1‑114 (MQ=255) gACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAg > 1:71196/1‑207 (MQ=255) cTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATc > 2:4748/1‑275 (MQ=255) tATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCg > 8:285672/1‑130 (MQ=255) tGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcgcg > 5:76151/1‑138 (MQ=255) tGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCcgcg < 6:76151/138‑1 (MQ=255) gAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCga < 7:1003564/100‑1 (MQ=255) ctGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaa < 6:376412/93‑1 (MQ=255) gCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGa > 5:334677/1‑148 (MQ=255) aT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCccgccg < 8:864928/110‑1 (MQ=255) aT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCccgccg > 7:864928/1‑110 (MQ=255) atcaATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGCTTAACCCCgaa < 5:409640/83‑1 (MQ=255) tcaATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCgt‑gtg > 6:170926/1‑122 (MQ=255) tcaATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCgt‑gtg < 5:170926/122‑1 (MQ=255) tcaATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCa > 8:1002562/1‑148 (MQ=255) caATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTAt < 5:501438/124‑1 (MQ=255) caATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTAt > 6:501438/1‑124 (MQ=255) caAGACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCAt > 8:553486/1‑148 (MQ=255) aactacaccGGTAAAGGTGGGTGGAACACGTTCGTCAGCGACGTATGTAAAGCCTGGCTTTGGGGGTTAATCCCGAAAGTAAACCGTGAAATCCCCGATCTCGAATTTCGGTGG‑GGGTAGCAAATGGAGCGGTACTTTCCGCATCTTACAAGGTCCATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCAAACCTGCGACATAGGGATGGCGTGATGGTCGGGCGGGAGGCGTATCAGAATCCGGGGATCCTTGCGGCGGTAGACCGGga < 4:534349/268‑1 (MQ=255) tttCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCTTGACTTTCCGCATCTTACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCCTGATGTCCGGGCGCGAGGCGTCTCAGAATCCGGGTATTCTGGCGGCGGTAGTCCgggtgc > 1:109223/1‑270 (MQ=255) tCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTTCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACAGGGAGTTCt > 2:112223/1‑274 (MQ=255) tCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTAACAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTGGGGCGAGAGGCGTATACGAATCCGGGTATGAGGGCGGCGGTGGACCg > 1:8798/1‑266 (MQ=255) ggCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGa < 6:334677/148‑1 (MQ=255) gCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGc > 6:1045826/1‑120 (MQ=255) aGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTACCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACg > 8:359264/1‑144 (MQ=255) aGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTACCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACg < 7:359264/144‑1 (MQ=255) gagaTGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTAt < 6:522541/146‑1 (MQ=255) gagaTGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCa > 5:1011612/1‑148 (MQ=255) gaTGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCgt‑g < 8:1049943/89‑1 (MQ=255) ttCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCg < 5:710733/126‑1 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggt < 5:712638/133‑1 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACggt > 6:712638/1‑133 (MQ=255) catcatCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGATCGAATATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTCTTCTGGCGGGTGTAGACCGGGAGTACTTTGGTTTCTCGGTTAcggtagacggta > 4:432250/1‑264 (MQ=255) atcatcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGt < 5:571212/121‑1 (MQ=255) atcatcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTgg > 7:951389/1‑148 (MQ=255) catcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTAt < 4:138087/205‑1 (MQ=255) catcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGCTCTTTGGTTCCTCGGATACCGATGCCGATACgg > 2:536908/1‑275 (MQ=255) tcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAaga > 7:1011578/1‑148 (MQ=255) tcCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAaga > 6:828711/1‑148 (MQ=255) cgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCa < 8:693061/131‑1 (MQ=255) cgcacgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCt > 6:190923/1‑138 (MQ=255) acgTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCaaa < 8:951389/148‑1 (MQ=255) ccgaaaGCCAGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATg < 8:120189/105‑1 (MQ=255) gTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCAGCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTg < 2:525476/275‑1 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTg > 8:1068668/1‑86 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTg < 7:1068668/86‑1 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACtt < 5:429132/90‑1 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTc > 6:119746/1‑92 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTc < 5:119746/92‑1 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAaga > 6:971889/1‑138 (MQ=255) tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAaga < 5:971889/138‑1 (MQ=255) aaaGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCaa < 6:1088648/143‑1 (MQ=255) aaaGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCaa > 5:1088648/1‑143 (MQ=255) aaaGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTAGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTgcg < 2:42536/275‑1 (MQ=255) gCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACAcc < 6:423694/148‑1 (MQ=255) tGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGc > 7:996521/1‑148 (MQ=255) tGGCTTTCGGGGAAAAGCCAGAAAGAAAACAGTGAAAACCCGCCGCTAGAGTGTCCGCGTAGTG‑ATCAACGGAAGCGTGACTTTCCGCATCTGAGAATGTCGAATAACGGTGGTATCAAGTCGCGGGAAGAGGCCAAAGCACACCTGCAACATAGGGATGGCGGGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGGATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCa < 4:384392/274‑1 (MQ=255) ttcTTTCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCa > 8:70824/3‑148 (MQ=255) gtcTTGCGGGGTTAAGCCACAAAAAAACCCGGGAAACCCGGCCGCTAGATTTGCCGCGT‑TTGTAGCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATAAACGGTGGGATCAAGTCGCTGGAGGAGGCCAAAGCACACCGGCAGCATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATg < 4:458278/273‑1 (MQ=255) ggCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCa > 5:313683/1‑148 (MQ=255) gCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCacac > 1:111704/1‑142 (MQ=255) ttCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTaa < 7:555199/102‑1 (MQ=255) ttCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAAcgt < 8:1011578/148‑3 (MQ=255) ttCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACat > 5:836124/1‑148 (MQ=255) ttCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGAt > 7:1072604/1‑46 (MQ=37) ttCGGGCTTAACCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGa > 7:4638/1‑123 (MQ=255) cGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACatat < 5:828711/148‑1 (MQ=255) ggggTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCAGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCg < 2:290795/274‑1 (MQ=255) | GGATATGATGAGATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTTCTCTGCGAT‑TTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGT‑GTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCG > NC_000913/4261978‑4262520 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |