| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,388,496 | T→A | 7.8% | Q292L (CAG→CTG) | mscM ← | mechanosensitive channel protein, miniconductance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,388,496 | 0 | T | A | 7.8% | 403.6 / 20.2 | 121 | Q292L (CAG→CTG) | mscM | mechanosensitive channel protein, miniconductance |
| Reads supporting (aligned to +/- strand): ref base T (56/52); new base A (4/5); total (62/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-03 | |||||||||||
Only 100 of 134 total aligned reads displayed.
AGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAG > NC_000913/4388235‑4388756 | aGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTCCCCCCGCAGCGCTTCGCCGACCAGCTTGGCAGATCCCCACCATTGCGACTGTTCACGCACCGTATTCCAAGCCCGCCGGACCGGTAACGTCCGGCCGTCACCCt > 4:482386/1‑274 (MQ=255) gTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTggt > 2:153706/1‑273 (MQ=255) gCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAg < 2:181968/275‑1 (MQ=255) gggattcaggtgCTGACGGTCGGCCTGAGGAGTTTTCGGGAGGAGCGGCCTTTTATTAAGACAAACCTGATAACGTACCCGGGGAACAGGCAACTGGGCCATGGCGGAATCATGCGGGGGTGGTGTCGGAATGTCCGGCCGCCGTGCCACCTGCGCCCGAAGCGCTTCGCCGAGCAGATTGGACGATCCCGGAAATTGCGACTGTTCACGCAGCGTATTCAACGCCGGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGGGAGGGaa < 4:242518/264‑1 (MQ=255) cGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgcg < 2:140542/273‑1 (MQ=255) cTGATGAATTTGCCGTAGCATCGGCTGTTTATTAAGCAGCTCCTCATAACGTAACCGTGGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCCGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTGCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGt < 1:39906/274‑1 (MQ=255) tttATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTGGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGt < 2:524497/275‑1 (MQ=255) gttattaAGTAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGt < 2:32167/274‑1 (MQ=255) aTCCTCATAACGTAATCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTGGATTCAAAGCCGCCGAACGTTCGCGGTTTACTTTGAAtt > 2:92758/1‑275 (MQ=255) aTCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa > 2:142714/1‑252 (MQ=255) aTCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGACGCAACCAGATCCATCCGCTGCCCCTGTTGATTCCAAGCCGCCGATAGTTcg > 4:32166/1‑259 (MQ=255) tCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTct < 4:560442/178‑1 (MQ=255) gacagaCGGAACCTGTGCACACGCAACTGCGCCCTTTCGTTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgcg < 1:1596/269‑1 (MQ=255) cGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGATGCACCGAGATCCATCCGCTGCGCCTGTTCATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGCATTGCGcgacga > 1:98243/1‑274 (MQ=255) tAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTACTTTTGAATTGCGCGACGatat > 2:258957/1‑275 (MQ=255) aCCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 4:150253/237‑1 (MQ=255) aCCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGCTGTGGTTTCGGCATTTCAGGCAGCCGTGCCACCCGTCCCCGCAGCGCTTCGCCGAGCAGATTGCACGATCCCAGCCATTGAGACTGTTCACGCAGCGTACTCAACGCCTGCCGGACCTGTAACGTCCGGCTGGCCACCTGAGTCGGCTGCTAGGCACCGGGACCCCCCCGCCGCCGCCGGTGGTTCAAGACCGCCACTAGTTCCGGGTACATGTTGGATTgcccacccataga > 4:27855/1‑263 (MQ=255) tttCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGCCGGTATCTTTCGGCCAATCGG‑CGCTGGTTTCt > 2:148626/1‑275 (MQ=255) ggTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTctggc > 6:445398/1‑143 (MQ=255) gtttCAAGCTGTTGTGGTTTCGGCATGTCCGGCAGCCGGGCCACCTGGGCCCCCCGGGCTTCGCCGGCCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAg < 4:103914/272‑1 (MQ=255) gTATCAAGCTGGTATGGTTTCGCCACTTCCGGCAGCCGTGCCCACTGCGCCCGGAGCGCTACGCCGAGCAGTGTGGACGATCCCAGCCATTGCGACTGTTCACGCCGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAg < 4:367104/275‑1 (MQ=255) aaGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa < 6:502440/148‑1 (MQ=255) aGCTGTTGTGGTTTCGGCATTTCTGGCAGCCGTGCCACCTGCGCCCGCCGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCCGTAACGTCTGGCTGNCAGCCTTCCGCTGTTGCCAGGCCACGAGATCCATCCGCTGCGCCTCTTGTTTCCAAGCCCCCGCTATTTCGCGGTTAATTNGGAATTTCGCGCCGACACCTTTCGGNAAAGACG‑CGCTCTTTTCTTccccccatt > 3:91835/1‑267 (MQ=255) gttgtGGTTTCGGCGTTTCCGGCCGCCGTGCCACCTGCGCCCGTCGCGTTTCGCCGGGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCGGCGGATTCAACGCCTGCCGGACCTGTAACGCCTGGCTGGCAGCCTGGCGCTGTTGCGAGGCAACGGGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTa < 4:191015/275‑1 (MQ=255) gttgtGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAGCGTCAGGCTGCCAGCCAGACgtta < 6:578788/148‑4 (MQ=255) ggTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGt > 5:365055/1‑144 (MQ=255) tttCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTctg > 7:277638/1‑123 (MQ=255) ttCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCt > 4:41005/1‑245 (MQ=255) tCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTTATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAg > 4:484855/1‑274 (MQ=255) tCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAg > 4:479574/1‑274 (MQ=255) ggCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCTGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCCACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATCGTTCGCGGTTAATTTTGAATTTCGCGCCGCTATCTTTCGGCACATCGG‑CGCTGTTTTCTGCCCGCCATTCTGTACTTTCCCgc > 1:124884/1‑273 (MQ=255) ggCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAgcgc > 2:153216/1‑275 (MQ=255) gCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGttt < 2:41006/245‑1 (MQ=255) gCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGccag < 5:368413/121‑3 (MQ=255) gCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGccag > 6:368413/1‑119 (MQ=255) cAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTctggc < 5:494165/110‑1 (MQ=255) tGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc > 6:328914/1‑148 (MQ=255) cACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGcc < 7:144739/148‑1 (MQ=255) tGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccat < 7:8744/134‑1 (MQ=255) tGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccat > 8:8744/1‑134 (MQ=255) cGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGa > 8:213776/1‑102 (MQ=255) gcgcTTCGCCGTGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTaa < 1:87926/274‑1 (MQ=255) gcgcTTCCGCGGGCAGATGGGGCAGTCCCAGCGACGGCGACGGTTCACGAAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCGGACGCTGTTGCGAGGCAACGCGATCCATCCGCGGCGCCGGTTGATTCAAAGGCGCCGATAGTTCGCGGTTAATTTTGAATTGCGAGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCGGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAAt < 4:302952/275‑1 (MQ=255) cgcTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa > 7:164073/1‑97 (MQ=255) cgcTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa < 8:164073/97‑1 (MQ=255) cGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAg > 6:729300/1‑148 (MQ=255) aGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTg > 6:1013556/1‑86 (MQ=255) aGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccat < 6:320368/113‑1 (MQ=255) aGCAGATTGGACGATCCCAGCCATTGCGACTGGTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccat > 5:320368/1‑113 (MQ=255) ttGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGt < 5:8815/87‑1 (MQ=255) ttGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCg > 6:200048/1‑91 (MQ=255) ggACGCTCCCCGCCTTTGCGACTGGTCACGCAGCGTATTCAACGCCCGCCGGACCTGTAACTTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCa < 4:153705/275‑1 (MQ=255) ggACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTctgg > 5:1072872/1‑67 (MQ=255) ggACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgag > 7:480727/1‑99 (MQ=255) ggACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgag < 8:480727/99‑1 (MQ=255) aCGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTctgg > 8:749178/1‑65 (MQ=255) aTCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa < 5:1075000/90‑1 (MQ=255) aTCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa > 6:1075000/1‑90 (MQ=255) aTCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg < 6:822362/123‑1 (MQ=255) cccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaa > 6:446196/1‑119 (MQ=255) cccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaa < 5:446196/119‑1 (MQ=255) cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGccaga > 7:192004/1‑67 (MQ=255) cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa < 8:192004/70‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 5:61984/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 8:795593/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 5:53892/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga > 8:489190/1‑90 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga > 7:795593/1‑90 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga > 6:53892/1‑90 (MQ=255) gCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTg < 5:729300/148‑1 (MQ=255) tGCGACTGTTCTCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGAAAAGAGCGCCCTCCGCTGCGCCTGTTGATTCAAAGCCGCCGCTAGTTCGCGGTTAATTTTGCAGTGCGCGACGATAGCTTACGGCAAAGCGGACGGT‑TTTTCTTGCAGCAATACGGTACTTTCCAGCGCCCGCTCAGACCCAAGCTGACGTTGTCTGTTTAATTGATTGCGTATGGCCGGCAAATAAGCATCCCGtt > 4:326227/1‑274 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcga < 7:773806/148‑1 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAAt > 2:248948/1‑240 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTg > 2:362347/1‑275 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTg > 2:396942/1‑275 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTg > 4:53903/1‑275 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTggatg > 2:485141/1‑271 (MQ=255) ttCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAATCAAAGCCGCCGATAGTTCGCGGTTCATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTCCTTTCCAGACCCCGCTCCGCCTCAAGCCGACGTTGGCTGTTTAATTGATTACCGAAGGCCTGCAAAACCGAATCCAGTTGCTGGctc > 4:268399/1‑274 (MQ=255) tCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 6:75184/109‑1 (MQ=255) tCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg > 5:75184/1‑109 (MQ=255) cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGctct > 2:197619/1‑273 (MQ=255) cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGNGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCCCTCCGCNCCCAGCGGACGTTGGCTGTTTTATTGATTAAGCAAGGCCTCCCAANANCCCTCCCATTTCTTGCTCCCttttttct > 3:50565/1‑272 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGTGGGCAGCCTGACGCGGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGct < 2:327934/275‑1 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGct < 4:396941/275‑1 (MQ=255) aaccGCCGGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGATGTCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGct < 2:56930/272‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTg > 8:618612/1‑79 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTg < 7:618612/79‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGt < 6:1054092/98‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGt > 5:1054092/1‑98 (MQ=255) cGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgc > 5:241549/1‑118 (MQ=255) cGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgc < 6:241549/118‑1 (MQ=255) cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGCTTACGCAAGGCCTGCAAATACGCCTCCCATTGCTGGCTCCTTTTTTccgcccgcctctaac > 4:515113/1‑268 (MQ=255) ggACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAg < 1:91835/275‑1 (MQ=255) tGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAtt > 6:35825/1‑68 (MQ=255) tGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAtt < 5:35825/68‑1 (MQ=255) aaCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt < 8:342589/116‑1 (MQ=255) aaCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt > 7:342589/1‑116 (MQ=255) cGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCgg > 8:692989/1‑125 (MQ=255) cGTCTGGCTGGCAGCCTGACGCTGTCGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCgg < 7:692989/125‑1 (MQ=255) ctggctggCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAAt > 7:546375/1‑142 (MQ=255) | AGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAG > NC_000913/4388235‑4388756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |