| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,388,508 | T→A | 5.4% | Q288L (CAG→CTG) | mscM ← | mechanosensitive channel protein, miniconductance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,388,508 | 0 | T | A | 5.4% | 446.2 / 12.6 | 132 | Q288L (CAG→CTG) | mscM | mechanosensitive channel protein, miniconductance |
| Reads supporting (aligned to +/- strand): ref base T (63/60); new base A (2/5); total (65/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
AGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTAT > NC_000913/4388235‑4388778 | aGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTCCCCCCGCAGCGCTTCGCCGACCAGCTTGGCAGATCCCCACCATTGCGACTGTTCACGCACCGTATTCCAAGCCCGCCGGACCGGTAACGTCCGGCCGTCACCCt > 4:482386/1‑274 (MQ=255) tGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCCGCCTGACGCTGTTGCGa > 2:291213/1‑275 (MQ=255) gCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAg < 2:181968/275‑1 (MQ=255) gggattcaggtgCTGACGGTCGGCCTGAGGAGTTTTCGGGAGGAGCGGCCTTTTATTAAGACAAACCTGATAACGTACCCGGGGAACAGGCAACTGGGCCATGGCGGAATCATGCGGGGGTGGTGTCGGAATGTCCGGCCGCCGTGCCACCTGCGCCCGAAGCGCTTCGCCGAGCAGATTGGACGATCCCGGAAATTGCGACTGTTCACGCAGCGTATTCAACGCCGGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGGGAGGGaa < 4:242518/264‑1 (MQ=255) cGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgcg < 2:140542/273‑1 (MQ=255) caggtgtaTTTGACGGAGGAGGGGCTGTTGTTTAAGCATATCCTCATGATGTAACGGTGTCACACGCAAGTGGGCGATTGCGTGCTCAAGCTTTTGTGGTTGCGGCATTGCCGGCAGCCGTGCAACCTGCGCCCGCAGCGCGTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGt < 4:290354/267‑1 (MQ=255) cTGATGAATTTGCCGTAGCATCGGCTGTTTATTAAGCAGCTCCTCATAACGTAACCGTGGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCCGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTGCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGt < 1:39906/274‑1 (MQ=255) atgaATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAt > 2:191016/1‑275 (MQ=255) agagacagtttttaAACAGATCCCTATAACGTACCCGTTGCACACGGAACTGCGCCATTTCGGTATCAGGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGGGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGt < 4:142713/264‑1 (MQ=255) tttATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTGGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGt < 2:524497/275‑1 (MQ=255) aTCCTCATAACGTAATCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTGGATTCAAAGCCGCCGAACGTTCGCGGTTTACTTTGAAtt > 2:92758/1‑275 (MQ=255) gacagaCGGAACCTGTGCACACGCAACTGCGCCCTTTCGTTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgcg < 1:1596/269‑1 (MQ=255) cGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGATGCACCGAGATCCATCCGCTGCGCCTGTTCATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGCATTGCGcgacga > 1:98243/1‑274 (MQ=255) cGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgcc > 2:150254/1‑237 (MQ=255) tAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTACTTTTGAATTGCGCGACGatat > 2:258957/1‑275 (MQ=255) aCCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 4:150253/237‑1 (MQ=255) aCCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGCTGTGGTTTCGGCATTTCAGGCAGCCGTGCCACCCGTCCCCGCAGCGCTTCGCCGAGCAGATTGCACGATCCCAGCCATTGAGACTGTTCACGCAGCGTACTCAACGCCTGCCGGACCTGTAACGTCCGGCTGGCCACCTGAGTCGGCTGCTAGGCACCGGGACCCCCCCGCCGCCGCCGGTGGTTCAAGACCGCCACTAGTTCCGGGTACATGTTGGATTgcccacccataga > 4:27855/1‑263 (MQ=255) aactcggaaCTGTGCCATGTCGGTATCAAGCTGTTGTGGTGTCGGCAGTTCCGGCAGCCTTGCCACCTGAGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGTGACTGTTCACGCAGCGTAGTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCa < 2:487194/268‑1 (MQ=255) tttCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGCCGGTATCTTTCGGCCAATCGG‑CGCTGGTTTCt > 2:148626/1‑275 (MQ=255) gtttCAAGCTGTTGTGGTTTCGGCATGTCCGGCAGCCGGGCCACCTGGGCCCCCCGGGCTTCGCCGGCCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAg < 4:103914/272‑1 (MQ=255) aaGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa < 6:502440/148‑1 (MQ=255) gttgtGGTTTCGGCGTTTCCGGCCGCCGTGCCACCTGCGCCCGTCGCGTTTCGCCGGGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCGGCGGATTCAACGCCTGCCGGACCTGTAACGCCTGGCTGGCAGCCTGGCGCTGTTGCGAGGCAACGGGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTa < 4:191015/275‑1 (MQ=255) gttgtGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAGCGTCAGGCTGCCAGCCAGACgtta < 6:578788/148‑4 (MQ=255) ggTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGt < 6:365055/144‑1 (MQ=255) ggTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGt > 5:365055/1‑144 (MQ=255) ttCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCt > 4:41005/1‑245 (MQ=255) tCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTTATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAg > 4:484855/1‑274 (MQ=255) tCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAg > 4:479574/1‑274 (MQ=255) ggCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCTGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCCACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATCGTTCGCGGTTAATTTTGAATTTCGCGCCGCTATCTTTCGGCACATCGG‑CGCTGTTTTCTGCCCGCCATTCTGTACTTTCCCgc > 1:124884/1‑273 (MQ=255) ggCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAgcgc > 2:153216/1‑275 (MQ=255) gCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGttt < 2:41006/245‑1 (MQ=255) tGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc > 6:328914/1‑148 (MQ=255) tGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccat > 8:8744/1‑134 (MQ=255) cgcCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCAATTGCGACTGTTCACGCAGCGTATTCAAGGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTgg < 1:75324/274‑1 (MQ=255) cGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGa < 7:213776/102‑1 (MQ=255) gcgcTTCGCCGTGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTaa < 1:87926/274‑1 (MQ=255) gcgcTTCCGCGGGCAGATGGGGCAGTCCCAGCGACGGCGACGGTTCACGAAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCGGACGCTGTTGCGAGGCAACGCGATCCATCCGCGGCGCCGGTTGATTCAAAGGCGCCGATAGTTCGCGGTTAATTTTGAATTGCGAGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCGGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAAt < 4:302952/275‑1 (MQ=255) cgcTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa < 8:164073/97‑1 (MQ=255) cgcTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa > 7:164073/1‑97 (MQ=255) cGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAg > 6:729300/1‑148 (MQ=255) aGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTg < 5:1013556/86‑1 (MQ=255) aGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTg > 6:1013556/1‑86 (MQ=255) aGCAGATTGGACGATCCCAGCCATTGCGACTGGTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatccat > 5:320368/1‑113 (MQ=255) ttGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGt > 6:8815/1‑87 (MQ=255) ttGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCg < 5:200048/91‑1 (MQ=255) ggACGATCCTAGCAATGGGGCCGGTTACCGCGGCGGATTAAACGCCTGCCGGACCTGTAACGTCGGGCTTGCAGCTTGACGCGGTTGCGAGGAAACGGGAGCCATCCGCTGCGCCGGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCa < 4:363501/275‑1 (MQ=255) ggACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgag < 8:480727/99‑1 (MQ=255) aTCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa < 5:1075000/90‑1 (MQ=255) aTCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa > 6:1075000/1‑90 (MQ=255) aTCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAg > 5:822362/1‑123 (MQ=255) cccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaa < 5:446196/119‑1 (MQ=255) cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGa < 8:192004/70‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 5:61984/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 8:795593/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga > 6:53892/1‑90 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga > 7:795593/1‑90 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 7:489190/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga < 5:53892/90‑1 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACgaga > 8:489190/1‑90 (MQ=255) aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAAtt > 6:254114/1‑148 (MQ=255) ccATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGc < 7:89816/97‑1 (MQ=255) tGCGACTGTTCTCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGAAAAGAGCGCCCTCCGCTGCGCCTGTTGATTCAAAGCCGCCGCTAGTTCGCGGTTAATTTTGCAGTGCGCGACGATAGCTTACGGCAAAGCGGACGGT‑TTTTCTTGCAGCAATACGGTACTTTCCAGCGCCCGCTCAGACCCAAGCTGACGTTGTCTGTTTAATTGATTGCGTATGGCCGGCAAATAAGCATCCCGtt > 4:326227/1‑274 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcga < 7:773806/148‑1 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAAt > 2:248948/1‑240 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTg > 2:362347/1‑275 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTg > 2:396942/1‑275 (MQ=255) tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTg > 4:53903/1‑275 (MQ=255) tCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg > 5:75184/1‑109 (MQ=255) tCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGccgccg < 6:75184/109‑1 (MQ=255) cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGctct > 2:197619/1‑273 (MQ=255) cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGNGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCCCTCCGCNCCCAGCGGACGTTGGCTGTTTTATTGATTAAGCAAGGCCTCCCAANANCCCTCCCATTTCTTGCTCCCttttttct > 3:50565/1‑272 (MQ=255) cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCg > 2:103619/1‑275 (MQ=255) gTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCACATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGCAGTTGGCTGTTTAATTGATTACCCAAGGCCTGCACATCCGCATCCAGTTCCTTGCCCTCTTTTTCCGc > 4:218138/1‑275 (MQ=255) ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCgg < 5:328914/148‑1 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGTGGGCAGCCTGACGCGGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGct < 2:327934/275‑1 (MQ=255) cAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGct < 4:396941/275‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTg < 7:618612/79‑1 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTg > 8:618612/1‑79 (MQ=255) aCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGt < 6:1054092/98‑1 (MQ=255) cGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgc < 6:241549/118‑1 (MQ=255) cTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGCTTACGCAAGGCCTGCAAATACGCCTCCCATTGCTGGCTCCTTTTTTccgcccgcctctaac > 4:515113/1‑268 (MQ=255) ggaccgtgaaCGTCTGGGTGGCAGCCGTCCGCTGTTGCGAGGCAACGGGATCCATCCTCTGCGCCTGTTGATTCAAANCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAg < 3:124884/267‑1 (MQ=255) tGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAtt > 6:35825/1‑68 (MQ=255) aaCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt > 7:342589/1‑116 (MQ=255) aaCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt < 8:342589/116‑1 (MQ=255) cGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCgg > 8:692989/1‑125 (MQ=255) tcaggctgccAGCCAGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccg > 6:66800/4‑69 (MQ=38) tcaggctgccAGCCAGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccg < 5:66800/66‑1 (MQ=38) ctggctggCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAAt > 7:546375/1‑142 (MQ=255) ggctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAttt < 6:872044/87‑1 (MQ=255) ggctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAttt > 5:872044/1‑87 (MQ=255) gctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTg > 6:20289/1‑93 (MQ=255) gctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATACGCGGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTg < 5:20289/93‑1 (MQ=255) ggcggcCTGACCCTGTTGCGCGGTAACGAGATCAATCCGCTGGCCCTTTTGATTCAAAGCCGCCGTGAGTTCGCGGGTAATTTTGAATTGCGCGCCGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGt < 4:367855/275‑1 (MQ=255) cAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAg < 5:254114/148‑1 (MQ=255) cAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCTGCCAGCTCTGAGCGTACGCGCGCTACTTCCTGGTGGtt > 1:145513/1‑274 (MQ=255) cAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGtt > 2:439594/1‑274 (MQ=255) aGCCTGACGCGGTTGCGAGGCAACGAGTTCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTAt < 2:488983/275‑1 (MQ=255) tGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt > 7:75626/1‑98 (MQ=255) tGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt < 8:75626/98‑1 (MQ=255) | AGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGG‑CGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTAT > NC_000913/4388235‑4388778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |