Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,470,958 | G→T | 6.5% | intergenic (‑45/+28) | ridA ← / ← pyrI | enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,470,958 | 0 | G | T | 6.5% | 344.8 / 11.3 | 107 | intergenic (‑45/+28) | ridA/pyrI | enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit |
Reads supporting (aligned to +/- strand): ref base G (47/53); new base T (3/4); total (50/57) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-02 |
Only 100 of 110 total aligned reads displayed.
AACACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGT > NC_000913/4470684‑4471229 | aaCACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg > 2:218660/1‑275 (MQ=255) ggTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACCGCGGTAAAAAGTCTGGCTCATTTTAGGGAGcc > 4:102365/1‑275 (MQ=255) tAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGANCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGCTAGCTGCCGGTGCATTTTACGCCGAGATAGTTTTGCTCATGANTTCTCCTTTATTACACCGGTAAAAANTCTTGATCATTATATGGAgtcgacctttt > 3:92508/1‑265 (MQ=255) gATGTCGCCCACTTGCAGGCCAGGGGGTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatta < 4:370243/275‑1 (MQ=255) tGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc < 2:66211/275‑1 (MQ=255) gTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCCCATTTGTGGGAGCCAGCCTTTTTTTACCa > 4:217496/1‑275 (MQ=255) aaGGCCATCGGGTTCGACGATCGCTTTTACGTTAGCCAGCGACGGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCaattaa < 2:149912/273‑1 (MQ=255) ccAGCGGCTTCTACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGc < 2:50908/275‑1 (MQ=255) ggggtctCGACGATAGTTTTTGCGTTATCCAACGACGTACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAATGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAg < 4:469786/269‑1 (MQ=255) gCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTTCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCNACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATATGTTTTCTCATGCTTTCTNCTTTATTACAGCGGTAAACAGTCTTGCTACTTATATGGAGCCAGANTNTT‑TTATCAATCCGCaattaatttaccat > 3:37675/1‑269 (MQ=255) gCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACATCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAg > 2:95349/1‑275 (MQ=255) gCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGcac < 2:46375/275‑1 (MQ=255) gCTTCGACGAGCGATTTTACGTTATCCCGCGACGGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGcac < 1:17172/275‑1 (MQ=255) cTTTTACCCTATCCAGCGACTGCCCTTGCTGTGCGTCTACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCTTGATCTGACCTGAAGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGTTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGaa < 1:157627/275‑1 (MQ=255) ttACGTTATTCAGCGACTGAATGGCTCGGGCAGCCACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGaaaa < 2:294815/274‑1 (MQ=255) tCCGGCGACTGCAGGGGCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGGTTTCGGATTTACCGGGATCTGACCGGAGGGGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCttttt < 4:502622/274‑1 (MQ=255) tCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGCTCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCGGTCGCGATAGTTTTGCGCAGGCTTTCTCCTTTCTTCCAGCGTTCAACAGTCTAGCTCCTTATCTGGAGCCAGACTTTTATTAACAA‑CCGAACTTAATTGGCCGACACCCCACTAGGGGCAAACATTTTTTc > 4:89254/1‑275 (MQ=255) tCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAATTCTGGCTCATTATAGGGATCCAGACTTTTATTACCAA‑CCGCAATTAATTGGACAGGCCCACATTATGGGAAAACTCTTTTTc > 4:72303/1‑275 (MQ=255) ccAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca < 1:92508/275‑1 (MQ=255) ccAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGGTTTCGGATTTACCGGGATCTTACCGAAGGTGATGATCATATTGCCTAGATCAACGCCCTGTACGTAAGGACCGATATCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca < 2:16371/275‑1 (MQ=255) gACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCa > 4:337841/1‑237 (MQ=255) tGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGcac < 2:337842/237‑1 (MQ=255) tgCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGT‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAg < 2:311046/272‑1 (MQ=255) ctgCGACGTCGGCGGGTACGTCGCCCGGTTTCGGATTTACCGGGTTCTGACCGGAGGGGATGATCATATTGCCCAGAGCAACGCCCTGTACGTAAGGACCGATAGCGGCCGGTGCATTTTCCGTAGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 4:430710/272‑1 (MQ=255) tCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTCCAGCGGTAAAAAGTCTGGCTCATTCACGGGGGTCTGACTTTTATTCCCAA‑CCGGACTTAATTGGCCAGCCCCAATATAAGGGAAAAATTTTTTTCCAAGTATTTGCATTTGCGCCTGATATcct > 4:486674/1‑272 (MQ=255) ggATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAg < 5:543853/148‑1 (MQ=255) cggggggTGATGTTCAGATTTCCCTGTTCACTGCCCTGTCCGTAAGAGCCGATGTGTGCCGGTTCATTTTCCGTCCGGATAGTTTTGCTCATGATTTCTCCTTTATTACAGAGGTAAAAAGTCTGGCTAATTATAGGGAGCCAGGCTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc < 4:455164/270‑1 (MQ=255) ggAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAt < 2:217497/275‑1 (MQ=255) gAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTgg > 4:126981/1‑169 (MQ=255) gAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa > 2:137958/1‑273 (MQ=255) tgatgaTCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAg < 2:126982/169‑1 (MQ=255) atgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTACGTCGCGCTAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCGTTATAGGGGGCCAGACTTTTATTACCAA‑ACGCCATTAATTCGCCAACACCACATTATGGTAAAACTCTTTTTCACAGTATTTGCCTTTGAACGGGATACCCTTGGGCCGTTTTCACACGGACAAGCTGGATAAAACCGGTTCGGCCTGGCCGac > 4:447204/1‑274 (MQ=255) cATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGACAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGTAAACTCTTTTTCTCAGTATTTGCATTTGAGCGCGATATCATTGTCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAGGGCTGATAccg > 1:58904/1‑272 (MQ=255) tatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACCGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGCCTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCAACTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAACGCGATATCATTGGCCCTTTTTTGCACGGCCACCCTGGATGAAACCGTTTCGGCATGGcctatagcgtt > 4:349445/1‑264 (MQ=255) ttgcAACGCCCGGTACGAAAGGACCGCTAGGTGCCGGTGCATTTTCCGTCGCGATAGTTTTGTTCATGATTTTTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACCTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGGGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCggg < 4:31894/272‑1 (MQ=255) aTCAACGACCTTTACGTAAGGACCGTTAGCTTCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATGTCTCCTTGATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCggg < 2:486675/274‑1 (MQ=255) cGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACttt > 8:629064/1‑118 (MQ=255) cGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACttt < 7:629064/118‑1 (MQ=255) tGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt > 6:240981/1‑111 (MQ=38) tGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt < 5:240981/111‑1 (MQ=38) gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt > 7:325521/1‑101 (MQ=38) gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACt < 8:325521/101‑1 (MQ=38) aTAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGcc > 3:82908/1‑123 (MQ=255) tAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCaattaa < 8:502544/116‑1 (MQ=255) cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGca < 1:82908/123‑1 (MQ=255) tttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGcc < 5:490487/75‑1 (MQ=37) tttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGcc > 6:490487/1‑75 (MQ=37) tttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGc < 5:1104253/74‑1 (MQ=37) ccGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGcgcg < 1:37675/273‑1 (MQ=255) gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCCCATTGTCGATGCGCTCCGGCAGACTTGGGCGCGAttt > 2:358443/1‑275 (MQ=255) tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta < 5:977226/78‑1 (MQ=255) tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta > 6:977226/1‑78 (MQ=255) tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTCGCTCATTATAGGGAGCCAGa > 7:645509/1‑72 (MQ=255) tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTCGCTCATTATAGGGAGCCAGa < 8:645509/72‑1 (MQ=255) tagtttttGCTCCTGATTTATCCTTTATTACATGGGCAAAAAGGCTTGATCATTTGAAGGGCCCAGACTTTTATTACNAA‑CCGAAATTAATTGGCCAGCCCCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccac < 3:123892/271‑1 (MQ=255) tAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccac < 2:349446/274‑1 (MQ=255) cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt > 7:1045738/1‑58 (MQ=255) cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt < 8:1045738/58‑1 (MQ=255) tGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACt < 2:196885/266‑1 (MQ=255) tGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGtt > 1:67317/1‑274 (MQ=255) gATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGAACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTAc > 1:123892/1‑257 (MQ=255) ctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCttt > 3:29857/1‑103 (MQ=255) tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca < 1:29857/103‑1 (MQ=255) tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt < 8:518309/142‑1 (MQ=255) tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt > 7:518309/1‑142 (MQ=255) ttattaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCg > 4:554140/1‑171 (MQ=255) taCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAt < 2:554141/171‑1 (MQ=255) gCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTAc > 2:471028/1‑239 (MQ=255) gTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATATTTGTCGATACGGTTAACCGTGGCtt > 4:219157/1‑275 (MQ=255) tAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACccac < 4:471027/239‑1 (MQ=255) aggTCTGGTTCATTATAGGGAGCCAGACGTTTATGACCAA‑CCGCAATGAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGTTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcggc < 2:89255/273‑1 (MQ=255) aaGTCTGGCTCATTATAGGGAGCCAGACTTTTAGTACCAA‑CCTAAATTAATTGGCCAGCACCACATTATGGGAAAACTCTGTTTCACAGTATTTGCATTTGAGCGGGATATCTTTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAGGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcggc < 1:6132/275‑1 (MQ=255) atgcTGGCTCATTATAGGGAGCCAGACTGTTATCAACAA‑ACGTGAGTAAGTGTCCAGCACCTCATGATGTGAAAACTCTGTGTAACAGAATTTGCATTTGAGCGTCCTACCATTGGGGCGTTTTCGGACGGAAAAGCTGGATGAAACCTGTCCGGCATGGCGGATACGGTTGCTGTTCGGCCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCTCCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTgcggcg < 4:202246/272‑1 (MQ=255) cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTAt > 7:807352/1‑65 (MQ=255) cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTAt < 8:807352/65‑1 (MQ=255) tGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCAACCACTCCATAGTTGTCGAAACGGTTAACCGTGGCTTGgcgcgccaa > 4:483701/1‑272 (MQ=255) ggCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAt > 7:230650/1‑92 (MQ=255) ggCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATa > 4:45092/1‑274 (MQ=255) cTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCaattaat > 6:342980/1‑43 (MQ=255) cTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCaattaat < 5:342980/43‑1 (MQ=255) cTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg > 6:356216/1‑133 (MQ=255) cTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg < 5:356216/133‑1 (MQ=255) cATTATAGGGATCCAGCCTTTTATAATCAA‑CCACAATTAATTGGCCTGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGAGTTTTCGCACGGCTAAGCTGGATGAAACCGGTTCGGCa < 5:282913/148‑1 (MQ=255) cATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt < 7:779151/73‑1 (MQ=255) cATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt > 8:779151/1‑73 (MQ=255) cATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc < 8:672848/86‑1 (MQ=255) cATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa > 6:9969/1‑148 (MQ=255) cATTATAGGGAGCCAGACATTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc > 7:672848/1‑86 (MQ=255) ttATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGat < 7:94214/97‑1 (MQ=255) ttATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg > 7:733519/1‑148 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc > 7:731830/1‑113 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc < 8:731830/113‑1 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACg > 4:47233/1‑118 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg > 8:399845/1‑138 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg < 7:399845/138‑1 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg > 5:1003661/1‑148 (MQ=255) tataGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTTCGGCGCTTACAATGCCa > 2:508713/1‑275 (MQ=255) taGGGGGCCAAACTTTAATTACCAA‑CCGCAATTAATGTGCCCGCACCACATTATGGGAAAACTCTTTTTCACATTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGt < 4:137957/275‑1 (MQ=255) gggAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaa < 2:47234/118‑1 (MQ=255) ggAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTCCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGAt > 7:279512/1‑148 (MQ=255) | AACACGGTAGTTTTAACGATGTCGCCCACTTTCAGGCCAGCGGCTTCGACGATCGCTTTTACGTTATCCAGCGACTGACGTGCCTGTGCAGCGACGTCTGCCGGTACTTCGCCCGTTTTCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAA‑CCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTGTTCGGGCAGACCAGCACATTGTCGATGCGCTCCGGCAGACTTGGGCGCGATTTACCCACCACTTCATAGTTGTCGATACGGTTAACCGTGGCTTGCGGCGCATACAATGCCAGT > NC_000913/4470684‑4471229 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |