Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,621,622 G→A 5.2% intergenic (+20/‑147) deoD → / → yjjJ purine nucleoside phosphorylase 1; nicotinamide 1‑beta‑D‑riboside phosphorylase/putative protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,621,6220GA 5.2% 313.6 / 4.0 93intergenic (+20/‑147)deoD/yjjJpurine nucleoside phosphorylase 1; nicotinamide 1‑beta‑D‑riboside phosphorylase/putative protein kinase
Reads supporting (aligned to +/- strand):  ref base G (35/53);  new base A (2/3);  total (37/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

TTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCT  >  NC_000913/4621360‑4621892
                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                              
ttCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGa                                                                                                                                                                                                                                                                    >  2:61715/1‑275 (MQ=255)
   tACGCTCCGGACGGGGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGc                                                                                                                                                                                                                                                                 <  2:438805/275‑1 (MQ=255)
     ctctCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGcc                                                                                                                                                                                                                                                                >  4:430056/1‑274 (MQ=255)
     ctctCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCg                                                                                                                                                                                                                                                               <  2:34867/275‑1 (MQ=255)
     cgctcCGGACGGCGAAATGTTCGACGGGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGACTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCATGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCATTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCg                                                                                                                                                                                                                                                               <  2:114448/273‑1 (MQ=255)
          cGGAGGGCGAAATGTTCGAGTTGTTGGAAAAAACCGGGAGTCACGGCGTGGAAATGGATACGGCTGGTATCTACGGCGGCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCg                                                                                                                                                                                                                                                          <  4:270306/275‑1 (MQ=255)
               ggCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGgaga                                                                                                                                                                                                                                                     <  2:221902/275‑1 (MQ=255)
                      tggtctaCGTGATGGAAAAATACGGCATGCTAGGCGTGGAAATGGAAGCGGCGGGTATCTACGGCGTCGCTGCAGAATGTGGCGCGAAAGCCATGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTc                                                                                                                                                                                                                                               <  4:340095/268‑1 (MQ=255)
                                   gggaaaaTACGGCATTCTCGGGGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCGTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGt                                                                                                                                                                                                                                   <  2:478411/270‑1 (MQ=255)
                                       aaaTACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTAt                                                                                                                                                                                                                             <  2:430057/275‑1 (MQ=255)
                                          tACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCa                                                                                                                                                                                                                          <  4:431466/275‑1 (MQ=255)
                                                aTTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCAttttt                                                                                                                                                                                                                     <  2:439534/274‑1 (MQ=255)
                                                   ctcGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTtctct                                                                                                                                                                                                                 <  4:61714/275‑1 (MQ=255)
                                                                tGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAatat                                                                                                                                                                                                    <  2:473538/275‑1 (MQ=255)
                                                                                 tccGGCGTGGCTGCCGAATTGGGGCGGAAAGCCCGGACCACATGCCCCGTAGCTGCCCACACCCCAACTCACGAGCAGACCACTTCCGCTGGGCGTCAGACTACCTTCAACGCCATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAATGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTc                                                                                                                                                                                    <  4:453247/272‑1 (MQ=255)
                                                                                       gtcgtctcAGAATTTGGCGCGAAAGCCGTTACCGTCTGCACCGTATCTGACCACATCCGGACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCGTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGaa                                                                                                                                                                              <  4:230256/267‑1 (MQ=255)
                                                                                                    ttGGCCCGCAAGCCCTGACCTCCGTCCCCGGATCTGACCCCATCCGCACCCACGAGCAGACCACTGCTGCTGGCCGTCAGACTACCTTGAACGACATGATCAAAATCTCACTGGAATCCGTTCTGCGTGGCGATAACGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCGCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAAt                                                                                                                                                                <  4:296030/275‑1 (MQ=255)
                                                                                                                   tGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCg                                                                                                                                                                                                                                                                                >  8:378402/1‑148 (MQ=255)
                                                                                                                     aCCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGAt                                                                                                                                                                                                                                                                              <  7:378402/148‑1 (MQ=255)
                                                                                                                      ccATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGAtt                                                                                                                                                                                                                                                                             >  5:743342/1‑148 (MQ=255)
                                                                                                                       cATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGAt                                                                                                                                                                                                                                                                              >  5:426896/1‑146 (MQ=255)
                                                                                                                       cATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGAt                                                                                                                                                                                                                                                                              <  6:426896/146‑1 (MQ=255)
                                                                                                                        aTCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCa                                                                                                                                             <  2:182235/274‑1 (MQ=255)
                                                                                                                           tGCACCGAATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCtt                                                                                                                                                                                                                                                                        <  7:980822/148‑1 (MQ=255)
                                                                                                                            gCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAGATCGCACTGGAATCCGTTCAGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAAt                                                                                                                                        <  1:43186/275‑1 (MQ=255)
                                                                                                                            gCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       <  6:743342/148‑1 (MQ=255)
                                                                                                                                          cacaTCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       >  5:876072/1‑134 (MQ=255)
                                                                                                                                          cacaTCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       <  6:876072/134‑1 (MQ=255)
                                                                                                                                           acttCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCt                                                                                                                                                                                                                                                                         >  8:971985/4‑131 (MQ=255)
                                                                                                                                           acaTCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCt                                                                                                                                                                                                                                                                         <  7:971985/131‑1 (MQ=255)
                                                                                                                                            caTCCCCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATgag                                                                                                                         <  1:49272/274‑1 (MQ=255)
                                                                                                                                              tCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGc                                                                                                                                                                                                                                                                           <  7:160314/126‑1 (MQ=255)
                                                                                                                                              tCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGc                                                                                                                                                                                                                                                                           >  8:160314/1‑126 (MQ=255)
                                                                                                                                                cGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGa                                                                                                                                                                                                                                                             <  6:1093829/138‑1 (MQ=255)
                                                                                                                                                cGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGa                                                                                                                                                                                                                                                             >  5:1093829/1‑138 (MQ=255)
                                                                                                                                                cGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGgag                                                                                                                                                                                                                                                      >  6:370242/1‑145 (MQ=255)
                                                                                                                                                cGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGgag                                                                                                                                                                                                                                                      <  5:370242/145‑1 (MQ=255)
                                                                                                                                                            cAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       <  7:233761/116‑1 (MQ=255)
                                                                                                                                                            cAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       >  8:233761/1‑116 (MQ=255)
                                                                                                                                                                   cTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGcc                                                                                                                                                                                                                                                                          >  8:350607/1‑106 (MQ=255)
                                                                                                                                                                   cTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGcc                                                                                                                                                                                                                                                                          <  7:350607/106‑1 (MQ=255)
                                                                                                                                                                     gCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGc                                                                                                                                                                                                                                                 >  5:619182/1‑129 (MQ=255)
                                                                                                                                                                     gCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGc                                                                                                                                                                                                                                                 <  6:619182/129‑1 (MQ=255)
                                                                                                                                                                           gAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTta                                                                                                                                                                      >  4:260725/1‑198 (MQ=255)
                                                                                                                                                                               gTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTtaataa                                                                                                                                                                  <  2:260726/198‑1 (MQ=255)
                                                                                                                                                                                   gACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCtt                                                                                                                                                                                                                                                                        >  5:276427/1‑92 (MQ=255)
                                                                                                                                                                                   gACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCtt                                                                                                                                                                                                                                                                        <  6:276427/92‑1 (MQ=255)
                                                                                                                                                                                   gACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCt                                                                                                                                                                                                                                                                         <  5:401863/91‑1 (MQ=255)
                                                                                                                                                                                   gACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCt                                                                                                                                                                                                                                                                         >  6:401863/1‑91 (MQ=255)
                                                                                                                                                                                       aCCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAg                                                                                                                                                                                                                                                                  <  7:837470/94‑1 (MQ=255)
                                                                                                                                                                                       aCCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAg                                                                                                                                                                                                                                                                  >  8:837470/1‑94 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtgt                                                                                                                                                                                                                                                                      >  5:1058308/1‑87 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtgt                                                                                                                                                                                                                                                                      <  6:1058308/87‑1 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       <  5:945784/86‑1 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       >  7:305544/1‑86 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       <  8:305544/86‑1 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTtg                                                                                                                                                                                                                                                                       >  6:945784/1‑86 (MQ=255)
                                                                                                                                                                                          ttCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTg                                                                                                                                                                                                         >  6:1070721/1‑148 (MQ=255)
                                                                                                                                                                                           tCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCAATCGCCTtgc                                                                                                                                                                                                                                                                      <  5:574100/86‑2 (MQ=255)
                                                                                                                                                                                           tCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCAATCGCCTtgc                                                                                                                                                                                                                                                                      >  6:574100/1‑85 (MQ=255)
                                                                                                                                                                                               cGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCggg                                                                                                                                                                                                                                                        <  5:47449/96‑1 (MQ=255)
                                                                                                                                                                                               cGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCggg                                                                                                                                                                                                                                                        >  6:47449/1‑96 (MQ=255)
                                                                                                                                                                                                      aTCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTc                                                              >  2:54163/1‑275 (MQ=255)
                                                                                                                                                                                                       tCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCttt                                                                                                                                                                                                                                        <  7:359477/104‑1 (MQ=255)
                                                                                                                                                                                                       tCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCttt                                                                                                                                                                                                                                        >  8:359477/1‑104 (MQ=255)
                                                                                                                                                                                                           aaTCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCt                                                                                                                                                                                                                                                                         >  8:269085/1‑67 (MQ=255)
                                                                                                                                                                                                           aaTCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCt                                                                                                                                                                                                                                                                         <  7:269085/67‑1 (MQ=255)
                                                                                                                                                                                                            aTCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACtt                                                                                                                                                                                       >  5:402916/1‑148 (MQ=255)
                                                                                                                                                                                                                cACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCtttt                                                                                                                                                                                                                                       >  4:563739/1‑96 (MQ=255)
                                                                                                                                                                                                                 aCTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAg                                                                                                                                                                                  <  6:402916/148‑1 (MQ=255)
                                                                                                                                                                                                                  cTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCggg                                                                                                                                                                                                                                                        <  6:289565/77‑1 (MQ=255)
                                                                                                                                                                                                                  cTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCggg                                                                                                                                                                                                                                                        >  5:289565/1‑77 (MQ=255)
                                                                                                                                                                                                                    ggAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAg                                                                                                                                                                                                                                                            <  6:896185/71‑1 (MQ=255)
                                                                                                                                                                                                                    ggAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAg                                                                                                                                                                                                                                                            >  5:896185/1‑71 (MQ=255)
                                                                                                                                                                                                                    ggAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGt                                                                                                                                                                                                                                   <  2:563740/96‑1 (MQ=255)
                                                                                                                                                                                                                     gAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGaa                                                                                                                                                                              <  5:1070721/148‑1 (MQ=255)
                                                                                                                                                                                                                      aaTCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCa                                                                                                                                                                                                                          <  5:269574/103‑1 (MQ=255)
                                                                                                                                                                                                                      aaTCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCa                                                                                                                                                                                                                          >  6:269574/1‑103 (MQ=255)
                                                                                                                                                                                                                      aaTCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCAATCGc                                                                                                                                                                                                                                                                           <  5:896848/54‑1 (MQ=255)
                                                                                                                                                                                                                        tCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCttttt                                                                                                                                                                                                                                      >  7:543161/1‑89 (MQ=255)
                                                                                                                                                                                                                        tCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCttttt                                                                                                                                                                                                                                      <  8:543161/89‑1 (MQ=255)
                                                                                                                                                                                                                          cGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCt                                                                                                                                                                                                                                          >  8:128783/1‑83 (MQ=255)
                                                                                                                                                                                                                          cGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCt                                                                                                                                                                                                                                          <  5:75995/83‑1 (MQ=255)
                                                                                                                                                                                                                          cGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCt                                                                                                                                                                                                                                          >  6:75995/1‑83 (MQ=255)
                                                                                                                                                                                                                          cGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCt                                                                                                                                                                                                                                          <  7:128783/83‑1 (MQ=255)
                                                                                                                                                                                                                                   gggCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATc                                                                                                                                                                                                                                >  8:94539/1‑84 (MQ=255)
                                                                                                                                                                                                                                   gggCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATc                                                                                                                                                                                                                                <  7:94539/84‑1 (MQ=255)
                                                                                                                                                                                                                                                   ttGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTtaat                                                                                                                                                                    >  3:140021/1‑128 (MQ=255)
                                                                                                                                                                                                                                                       gtTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAAt                                                                                                                                                                <  1:140021/128‑1 (MQ=255)
                                                                                                                                                                                                                                                        tttCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGctga                                                                                                                 >  3:59699/1‑174 (MQ=255)
                                                                                                                                                                                                                                                            gCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGctgactga                                                                                                             <  1:59699/174‑1 (MQ=255)
                                                                                                                                                                                                                                                             tcacAAGGCAATCGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTc                                                                                                                                                                                    <  6:508266/99‑1 (MQ=255)
                                                                                                                                                                                                                                                             tcacAAGGCAATCGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTc                                                                                                                                                                                    >  5:508266/4‑102 (MQ=255)
                                                                                                                                                                                                                                                                  aGGGGATTGCCTTGGGAAGCCGGAGGGGGGGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTGTCTCCAGCTTGAATATTTTCGCTAGACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTAGCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCt  <  4:67539/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                              
TTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGCTCCGGCTTTTTAGTATCTATTCATTTTTCTCTCCAGCTTGAATATTTTCGCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCT  >  NC_000913/4621360‑4621892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: