Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 585,653 G→A 12.9% intergenic (‑20/‑404) ompT ← / → pauD DLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913585,6530GA12.9% 195.8 / 19.3 70intergenic (‑20/‑404)ompT/pauDDLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base A (5/4);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

GGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCA  >  NC_000913/585388‑585857
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                            
gggCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGa                                                                                                                                                                                                     <  1:122056/275‑1 (MQ=255)
 ggCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCTGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGAt                                                                                                                                                                                                    <  2:218394/275‑1 (MQ=255)
                                                                                                               tGTTTTTCCGCTCAGAGTGCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGGCGCGGGAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGGTCTCCATTCAATCGTTTTAATGAGTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATGTTGTTTAGTATTGAATGAGTATCACAACGCGCTATCTGTTTTTGGt                                                                                       <  1:44052/274‑1 (MQ=255)
                                                                                                                             gagTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTa                                                                                                                                                                                                         <  6:336861/146‑1 (MQ=255)
                                                                                                                             gagTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTa                                                                                                                                                                                                         >  5:336861/1‑146 (MQ=255)
                                                                                                                                    aaGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGtt                                                                                                                                                                                                            >  5:387698/1‑136 (MQ=255)
                                                                                                                                    aaGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGtt                                                                                                                                                                                                            <  6:387698/136‑1 (MQ=255)
                                                                                                                                                  cATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGAatg                                                                                                                                                                                              >  6:29811/1‑135 (MQ=255)
                                                                                                                                                  cATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGAatg                                                                                                                                                                                              <  5:29811/136‑2 (MQ=255)
                                                                                                                                                    tttATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGAat                                                                                                                                                                                               <  7:929354/133‑1 (MQ=255)
                                                                                                                                                    tttATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGAat                                                                                                                                                                                               >  8:929354/1‑133 (MQ=255)
                                                                                                                                                           tgtCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGAt                                                                                                                                                                                                    <  7:455786/121‑1 (MQ=255)
                                                                                                                                                           tgtCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGAt                                                                                                                                                                                                    >  8:455786/1‑121 (MQ=255)
                                                                                                                                                                     aaaCGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGaa                                                                                                                                                                                                >  7:812569/1‑115 (MQ=255)
                                                                                                                                                                     aaaCGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGaa                                                                                                                                                                                                <  8:812569/115‑1 (MQ=255)
                                                                                                                                                                        cGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGAt                                                                                                                                                                                                    >  8:209216/1‑108 (MQ=255)
                                                                                                                                                                        cGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGAt                                                                                                                                                                                                    <  7:209216/108‑1 (MQ=255)
                                                                                                                                                                          aTAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGt                                                                                                                                                                                                             <  5:362042/97‑1 (MQ=255)
                                                                                                                                                                          aTAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGt                                                                                                                                                                                                             >  6:362042/1‑97 (MQ=255)
                                                                                                                                                                          aTAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGa                                                                                                                                                                                                 <  8:914780/109‑1 (MQ=255)
                                                                                                                                                                          aTAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGa                                                                                                                                                                                                 >  7:914780/1‑109 (MQ=255)
                                                                                                                                                                           tAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAata                                                                                                                                                                                              <  5:726336/111‑1 (MQ=255)
                                                                                                                                                                           tAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAata                                                                                                                                                                                              >  6:726336/1‑111 (MQ=255)
                                                                                                                                                                            aaaGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGaa                                                                                                                                                                                                >  6:572714/1‑108 (MQ=255)
                                                                                                                                                                             aaGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGaa                                                                                                                                                                                                <  5:482296/107‑1 (MQ=255)
                                                                                                                                                                             aaGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCATTAAAACGATTGaa                                                                                                                                                                                                >  6:482296/1‑107 (MQ=255)
                                                                                                                                                                                tctcGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATg                                                                                                                                                                     >  7:724363/1‑131 (MQ=255)
                                                                                                                                                                                tctcGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATg                                                                                                                                                                     <  8:724363/131‑1 (MQ=255)
                                                                                                                                                                                          aGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATc                                                                                                              >  2:277049/1‑176 (MQ=255)
                                                                                                                                                                                              aaaGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACaa                                                                                                          <  4:277048/176‑1 (MQ=255)
                                                                                                                                                                                               aaGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTaa                                                                                                                                                                                                        <  8:669640/81‑1 (MQ=255)
                                                                                                                                                                                               aaGAGCTGATAGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTaa                                                                                                                                                                                                        >  7:669640/1‑81 (MQ=255)
                                                                                                                                                                                                      gATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttttt                                                                                                                                                                                    <  6:712531/94‑1 (MQ=255)
                                                                                                                                                                                                      gATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttttt                                                                                                                                                                                    >  5:712531/1‑94 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGaa                                                                                                                                                                                                <  8:440257/80‑1 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGaa                                                                                                                                                                                                >  7:440257/1‑80 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttttt                                                                                                                                                                                    >  8:56216/1‑92 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttttt                                                                                                                                                                                    <  7:56216/92‑1 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttt                                                                                                                                                                                      >  8:1125122/1‑90 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttt                                                                                                                                                                                      <  7:1125122/90‑1 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCACCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGa                                                                                                                                                                    >  8:993858/1‑108 (MQ=255)
                                                                                                                                                                                                        tCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCACCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGa                                                                                                                                                                    <  7:993858/108‑1 (MQ=255)
                                                                                                                                                                                                           cAATCGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTaa                                                                                                                                                                       <  6:925818/102‑1 (MQ=255)
                                                                                                                                                                                                           cAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTaa                                                                                                                                                                       >  5:925818/1‑102 (MQ=255)
                                                                                                                                                                                                               aGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGt                                                                                                                                                                                                             >  8:7561/1‑60 (MQ=255)
                                                                                                                                                                                                               aGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGt                                                                                                                                                                                                             <  7:7561/60‑1 (MQ=255)
                                                                                                                                                                                                                       tCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttttt                                                                                                                                                                                    >  7:57033/1‑77 (MQ=255)
                                                                                                                                                                                                                       tCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTAtttttt                                                                                                                                                                                    <  8:57033/77‑1 (MQ=255)
                                                                                                                                                                                                                        cAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTa                                                                                                                                                                        >  8:896807/1‑88 (MQ=255)
                                                                                                                                                                                                                        cAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTa                                                                                                                                                                        <  7:896807/88‑1 (MQ=255)
                                                                                                                                                                                                                              tATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGaa                                                                                                                                                                                                <  6:147691/58‑1 (MQ=255)
                                                                                                                                                                                                                              tATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGaa                                                                                                                                                                                                >  5:147691/1‑58 (MQ=255)
                                                                                                                                                                                                                               aTTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGa                                                                                                                                                                                                 >  6:699151/1‑56 (MQ=255)
                                                                                                                                                                                                                               aTTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGa                                                                                                                                                                                                 <  5:699151/56‑1 (MQ=255)
                                                                                                                                                                                                                                 tCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGTTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGt      >  4:330088/1‑241 (MQ=255)
                                                                                                                                                                                                                                    cAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGt                                                                                                                                 >  6:237271/1‑115 (MQ=255)
                                                                                                                                                                                                                                    cAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGt                                                                                                                                 <  5:237271/115‑1 (MQ=255)
                                                                                                                                                                                                                                     acaaGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATAttt                                                                                                                                    <  8:84359/109‑1 (MQ=255)
                                                                                                                                                                                                                                     aGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATAttt                                                                                                                                    >  7:84359/1‑111 (MQ=255)
                                                                                                                                                                                                                                     aGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGTTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCa  <  2:330089/241‑1 (MQ=255)
                                                                                                                                                                                                                                      gAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCaa                                                                                                                                                               >  8:527100/1‑83 (MQ=255)
                                                                                                                                                                                                                                      gAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCaa                                                                                                                                                               <  7:527100/83‑1 (MQ=255)
                                                                                                                                                                                                                                                ccGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTa                                                                                    >  6:385189/1‑148 (MQ=255)
                                                                                                                                                                                                                                                     tAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGttt                                                                                                                               >  6:1012380/1‑100 (MQ=255)
                                                                                                                                                                                                                                                     tAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGttt                                                                                                                               <  5:1012380/100‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaaaGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATc                                                                              <  5:385189/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGt                                                                                                                                 >  8:771533/1‑81 (MQ=255)
                                                                                                                                                                                                                                                                      aTCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGt                                                                                                                                 <  7:771533/81‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGt                                                                                                                            >  7:1071841/1‑86 (MQ=255)
                                                                                                                                                                                                                                                                      aTCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGt                                                                                                                            <  8:1071841/86‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGt                                                                                                                                 <  7:545725/78‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGt                                                                                                                                 >  8:545725/1‑78 (MQ=255)
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                            
GGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTTCTCCATTCAATCGTTTTAATGATTGAATATGTATTTTTTATATCTAACTTAATGAGTCAATTACATATTGCTCCACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCA  >  NC_000913/585388‑585857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑