| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 665,094 | G→C | 5.1% | P33A (CCT→GCT) | rlpA ← | septal ring protein, suppressor of prc, minor lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 665,094 | 0 | G | C | 5.1% | 502.9 / 13.5 | 149 | P33A (CCT→GCT) | rlpA | septal ring protein, suppressor of prc, minor lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (61/78); new base C (4/4); total (65/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
TTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTA > NC_000913/664823‑665355 | ttggTGATTCTGGCGTAGCTGGGGATCGGAGGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTg < 2:122587/272‑1 (MQ=255)ttAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGTGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTg < 2:102927/275‑1 (MQ=255) tAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTg > 2:350274/1‑274 (MQ=255) gaggtGGGGATCGGAAGCGTTGGGTGGGGGGCCGTAAGGGTTGTAGGGTCGAATGGTTCGCCAGAGGCCGGCAGGTTACTGCCGGGTTAGGCATCATAGATTGCCGCCAGGCCCGGCTGGCTAAATCGAGACGGATCCGGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGt < 4:65020/271‑1 (MQ=255) aGCGTTGGATGGGCTGCCGTCAGCGTTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCCCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTAGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcatc < 1:1569/275‑1 (MQ=255) gTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcatcat < 2:321403/274‑1 (MQ=255) ggATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt > 2:265537/1‑275 (MQ=255) ggtGGGCGGCCGTCAGCTGTGTCGGATCGAATGCTAGGCCAGAGACCGTCAGGTTACTGCCGGGTTCGGAATCATAGATTGCCGCCGGTCCCGCATGGATAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGAGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt < 2:314165/272‑1 (MQ=255) gCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTTTAGGGCCGTTACATACCGCCGGCTGCGGTACCCTTACCGTCTGTTGCTGACCATCCTCGCTTGTAcc > 4:403742/1‑274 (MQ=255) cGTAAGCTGTGTCGGACCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGGCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGcc < 2:533290/274‑1 (MQ=255) ggCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc > 2:326915/1‑275 (MQ=255) gCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCGGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCa < 2:309198/275‑1 (MQ=255) gCCGTCAGGGTACTGCCGGGTTCGGCATCATCGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc < 2:9979/274‑1 (MQ=255) gTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTAc < 2:330731/275‑1 (MQ=255) cTGCCGGGTTCGGCATCATGAATTCCCGCCAGTCCCGCCTGGCTAAATCGGGACGGATCCTGCACGAGTTTGTAGCTTTTTCCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCa < 4:529764/274‑1 (MQ=255) cTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGAACCCTGAACGATTTTTTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCa < 2:129787/274‑1 (MQ=255) gTTCGGCATAATTGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCAc < 2:269878/275‑1 (MQ=255) tcagAGATTGCCGCCAGTCCCGCATGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTaca < 1:64558/275‑1 (MQ=255) aTTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTCCGCCTTGCGCACCCCttcccagcct > 4:365032/1‑266 (MQ=255) ctccatccccGCCGGCCGAAATGGAGCCGGTGCCTGCACTATTTGGTTGCTGTTACCGGCGCGCTGGTAGTCCGGAGTTGCCGTAGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGTCTGCGGTACAATTACCGTCTGTTGGTGACCATCGTCGCGTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATGCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa < 4:317076/267‑1 (MQ=255) cgccATTCCCGCCTGGCTAAATCGAGACGGTTCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa < 2:234114/274‑1 (MQ=255) ccAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCttt > 2:320168/1‑268 (MQ=255) ccAGTCCCGCCTGGCTAAAGCGGTACGGATCCTGCACGATTTTGTAGCGTTGACCGTCGCGCTGGTAAGCCTTATTTGCCGGCGCGTTCAGGGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCGGCGGTACACTTACCGGCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGaca < 4:62853/274‑1 (MQ=255) tCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt < 2:446759/186‑1 (MQ=255) ccTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGCCTGCgg < 8:49100/148‑1 (MQ=255) cttgCTAATTCGTGCCGGGTCCTGCACGATTTTTTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtg < 4:265536/272‑1 (MQ=255) tAAATCGAGACGTGTCCTGCACGTTTTGTTAGCGTTTACCGTCGCGCTGGTAGTCCTGATTGGCCGTCGCGTTAAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACGTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgt < 4:296962/275‑1 (MQ=255) aTCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGCCTGCGGt > 8:800205/1‑140 (MQ=255) ttCCGGCACGGTTTTTTGGCTTTGACCGCCGCGCTGGTAATCCTGATTTGCCGTGGCGTTCAATGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACAt < 4:321424/273‑1 (MQ=255) aTCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACCTGCCGCGAGCATTCCTGCCGCGATGCAGATACCGAGCCACTGCTTACGCATTGCGCACATCTTACACCCTTTTCGACAACATTTTCCTGTGGGTATGGATTGACAtt > 4:193889/1‑275 (MQ=255) gtttGCACGAGTTGGTGGGTTTTACCGTGGCGAGGGTAATACGGATTTGCGGTCGCGTTCATGGGTTCCAAACGCGGGTCCGCCCCGCTAATTGAAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGCCGGTTGCTGACCATCAGCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa < 4:141014/272‑1 (MQ=255) cTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACtt < 8:574415/133‑1 (MQ=255) cACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGt > 4:211946/1‑139 (MQ=255) aCGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAgg < 7:582396/116‑1 (MQ=255) aCGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAgg > 8:582396/1‑116 (MQ=255) ttttGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGTTTGACATTACAATCCCGa > 1:117218/1‑274 (MQ=255) tttGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCg < 7:134563/148‑1 (MQ=255) tAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTg > 7:7510/1‑134 (MQ=255) tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt < 7:359613/141‑1 (MQ=255) tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt > 8:359613/1‑141 (MQ=255) tttACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACAttt > 4:2474/1‑231 (MQ=255) ccGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt < 2:2474/231‑1 (MQ=255) gcgggTATTCCTGATTTGGCGTCGGGTTCAGTGTGTGCAAACGCGGGTCCGCCCCGCAAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTCCACTTCCCTTCTGTTGCTGACCATCATCGCTGGTACATGCCGCGGGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAg < 4:101433/272‑1 (MQ=255) cTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTg < 8:17945/111‑1 (MQ=255) aaTCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACAGGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa < 1:37712/275‑1 (MQ=255) tCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGAtc < 4:326914/275‑1 (MQ=255) ccTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGa > 6:827641/1‑135 (MQ=255) ccTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGa < 5:827641/135‑1 (MQ=255) cTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcat > 7:672593/1‑113 (MQ=255) tttGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGc > 2:37919/1‑246 (MQ=255) gCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAAc < 2:193890/275‑1 (MQ=255) ccGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATc < 4:37918/246‑1 (MQ=255) gTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACtt < 8:730562/84‑1 (MQ=255) tCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGa > 6:210586/1‑138 (MQ=255) tCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGa < 5:210586/138‑1 (MQ=255) tCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGc > 6:96617/1‑89 (MQ=255) tCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGc < 5:96617/89‑1 (MQ=255) gTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTg > 8:36202/1‑148 (MQ=255) ggTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGa > 5:571475/1‑127 (MQ=255) ggTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGa < 6:571475/127‑1 (MQ=255) gTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCNCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGa < 4:545230/206‑1 (MQ=255) ttCGAACCGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGTCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGa < 2:56765/275‑1 (MQ=255) ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGc > 8:732958/1‑82 (MQ=255) ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGc < 7:732958/82‑1 (MQ=255) ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTg < 8:1121660/98‑1 (MQ=255) ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGaa > 4:42913/1‑220 (MQ=255) tCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTAc < 7:550686/71‑1 (MQ=255) tCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTAc > 7:166078/1‑148 (MQ=255) cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCTGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCg < 8:454447/134‑1 (MQ=255) cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCg > 7:454447/1‑134 (MQ=255) aaaCGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa < 2:42914/220‑1 (MQ=255) aaaCGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTGACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGa < 1:142875/243‑1 (MQ=255) cgcgGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTg < 8:166078/148‑1 (MQ=255) cgGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTgcg > 5:309899/1‑148 (MQ=255) cccGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcatcat < 7:298530/74‑1 (MQ=255) cccGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACcatcat > 8:298530/1‑74 (MQ=255) cctgtAATTTAAACTAAAGGGCCGTGAAATAACGCAGGATGCGGGACACTTACCTGTTGTTGCTGACCATCATCGATTGTTCAGGCCGCGAGCATTCCGGCCGCGAGGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGaa < 4:309842/271‑1 (MQ=255) cGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCt < 5:534618/57‑1 (MQ=255) cGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCt > 6:534618/1‑57 (MQ=255) gCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt < 6:749989/75‑1 (MQ=255) gCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCt > 5:749989/1‑75 (MQ=255) tAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGc > 6:227211/1‑148 (MQ=255) taaaCTATAGGGCCGTTACATACCGCAGGCGGCGGTACACTTACCGGCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATAACAATCCCGAACCCAGCCATCAGTACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCtt < 2:110736/272‑1 (MQ=255) cAACTATAGTGCCTTTACATCCCGCAGGCTGCGGTACACTTACCGTCTGTTTCTTACCATCATCGCGTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACCACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTtac < 1:158292/275‑1 (MQ=255) cAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGa < 7:988324/96‑1 (MQ=255) cTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGaca < 6:309899/148‑1 (MQ=255) ggCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATcc < 5:832399/96‑1 (MQ=255) ggCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATcc > 6:832399/1‑96 (MQ=255) ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtg > 7:182842/1‑148 (MQ=255) ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACCATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCCGAATACCGCTTACCATACCAATATTTa > 2:19830/1‑275 (MQ=255) tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAg > 5:63397/1‑90 (MQ=255) tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAg < 6:63397/90‑1 (MQ=255) tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACct > 6:301947/1‑114 (MQ=255) tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACct < 5:301947/114‑1 (MQ=255) tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTggg > 6:850734/1‑147 (MQ=255) tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTggg < 5:850734/147‑1 (MQ=255) tACCGCAGCCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCg < 8:290901/88‑1 (MQ=255) tACCGCAGCCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCg > 7:290901/1‑88 (MQ=255) cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGtgtg < 7:36202/148‑1 (MQ=255) gCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATa < 4:111390/240‑1 (MQ=255) | TTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCCGCCTGGCTAAATCGAGACGGATCCTGCACGATTTTGTAGCTTTTACCGTCGCGCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTA > NC_000913/664823‑665355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |