Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 106,831 T→A 7.4% I92N (ATT→AAT)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913106,8310TA 7.4% 778.2 / 39.4 225I92N (ATT→AAT) lpxCUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (114/94);  new base A (9/8);  total (123/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.22e-01

Only 100 of 242 total aligned reads displayed.

ATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGC  >  NC_000913/106560‑107098
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                           
atCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTg                                                                                                                                                                                                                                                                           >  2:344962/1‑274 (MQ=255)
                          cGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATcc                                                                                                                                                                                                                                                >  2:211259/1‑275 (MQ=255)
                                       ggTGTCGGGTTACTAACCGGCAAGAAAGTCACCCTGACGTTACGCCCTCCGCCGGCCAACACCTGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACgg                                                                                                                                                                                                                                   <  4:187503/275‑1 (MQ=255)
                                                   cacacagccAAGAAACGCACCTGGGCTTTACACCTGGCCCCGGTCAACACAGAGGTAATGTATCGTAGCACCGACTGTAATCCACCGGGATATTTCCCGTCCGAGGCCAAATCTGTGCGCTATACCATGCTCTGGACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAGCCGTAGAGCACCTCAATGCTTCTCTCGCGGGCTTGGGCAGCGAGAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc                                                                                                                                                                                                                        <  4:515904/266‑1 (MQ=255)
                                                    ctaacGGCAAGAAACTCCTCCTGGCGTTATGGCCGGGGCCGTCGTACACCTGGGCCAGCTTGCGTCGCACGGACTTGAATCGACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGGGATACCATGCTCTGTACGTTTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAAGGCTGCTCTCGCGGGCGTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTcc                                                                                                                                                                                                                       <  4:99728/270‑1 (MQ=255)
                                                    aTACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTcc                                                                                                                                                                                                                       <  4:211258/274‑1 (MQ=255)
                                                                           tcGTTACCCCCTGCGCCGGCCAAACCCCGTGTCATCTATCGTCGCACCGACTTGAATCCACCGTTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCCCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGc                                                                                                                                                                                               <  4:161063/274‑1 (MQ=255)
                                                                                             gCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCCCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGaa                                                                                                                                                                             >  2:520139/1‑275 (MQ=255)
                                                                                                                 tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGCCGAGTTGAACTGCGCCAAAAAATTTg                                                                                                                                                            >  1:37766/1‑272 (MQ=255)
                                                                                                                        aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCaa                                                                                                                                                  >  4:33767/1‑275 (MQ=255)
                                                                                                                         ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCCGCCACGCTCCGGTTGTATACCTGCCGCTAGACGCCGGTATCGACGAGGTGAACTGCGCCAAAAAAATGGTTCGGATCaag                                                                                                                                                 >  4:458857/1‑274 (MQ=255)
                                                                                                                            aCTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAt                                                                                                                                                                                                                                                                             <  8:20121/148‑1 (MQ=255)
                                                                                                                            aCTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAt                                                                                                                                                                                                                                                                             <  7:847491/148‑1 (MQ=255)
                                                                                                                             cTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAtt                                                                                                                                                                                                                                                                            >  7:1126784/1‑148 (MQ=255)
                                                                                                                               tGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGt                                                                                                                                                                                                                                                                          <  7:1083356/148‑1 (MQ=255)
                                                                                                                               tGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGt                                                                                                                                                                                                                                                                          >  8:1083356/1‑148 (MQ=255)
                                                                                                                                 aaTCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTa                                                                                                                                                                                                                                                                        >  7:363105/1‑148 (MQ=255)
                                                                                                                                   tCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATc                                                                                                                                                                                                                                                                      >  8:428689/1‑148 (MQ=255)
                                                                                                                                            aGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAt                                                                                                                                                                                                                                                                             <  5:505613/132‑1 (MQ=255)
                                                                                                                                            aGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAt                                                                                                                                                                                                                                                                             >  6:505613/1‑132 (MQ=255)
                                                                                                                                                    cGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGa                                                                                                                                                                                                                                                     >  6:746261/1‑148 (MQ=255)
                                                                                                                                                        cGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGaaa                                                                                                                                                                                                                                                   <  8:775292/146‑1 (MQ=255)
                                                                                                                                                        cGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGaaa                                                                                                                                                                                                                                                   >  7:775292/1‑146 (MQ=255)
                                                                                                                                                              cAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGcgccg                                                                                                                                                                                                                           <  4:171611/164‑1 (MQ=255)
                                                                                                                                                               aaaTCTGTGAGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGATCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCa                                                                                                                                                                                                                                          <  5:726794/148‑1 (MQ=255)
                                                                                                                                                                  tCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATgg                                                                                                                                                                                                                                       >  6:931692/1‑148 (MQ=255)
                                                                                                                                                                      tgtgTGATTCAACGCTCAGAAAGTGTTGGGTCAAAGTGCCGGATGTCCGGATTTCAACCGTGGAGAACCCGAATGCTGTGCTCGCGAGCTAGGGCATCGATAAGATTGTTATCGAAGTTAACGCGCCAGAAATGCCGCTCATGGACGGGAGGTTCGCTCCGTTTGTATACCTGCTGCTTGACGCCGATATCGACGAGTTAAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGGCTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAg                                                                                                    <  4:36153/272‑1 (MQ=255)
                                                                                                                                                                           gATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGa                                                                                                                                                                                                                                                                    <  6:204558/110‑1 (MQ=255)
                                                                                                                                                                           gATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGa                                                                                                                                                                                                                                                                    >  5:204558/1‑110 (MQ=255)
                                                                                                                                                                                cATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTAt                                                                                                                                                                                                                                                                       >  6:722136/1‑102 (MQ=255)
                                                                                                                                                                                cATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGa                                                                                                                                                                                                                                             <  7:317256/128‑1 (MQ=255)
                                                                                                                                                                                cATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCt                                                                                                                                                                                                                         <  5:931692/148‑1 (MQ=255)
                                                                                                                                                                                 aTGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAtt                                                                                                                                                                                                                                                                            <  8:399545/96‑1 (MQ=255)
                                                                                                                                                                                 aTGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTc                                                                                                                                                                                                                        >  7:404198/1‑148 (MQ=255)
                                                                                                                                                                                        gTACGTGTCTGGTCAACGAGCATGATGTACTGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAAcgcg                                                                                                                                                                                                                                                          <  6:805689/107‑1 (MQ=255)
                                                                                                                                                                                        gTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAAcgcg                                                                                                                                                                                                                                                          >  5:805689/1‑107 (MQ=255)
                                                                                                                                                                                         tACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGc                                                                                 >  4:277118/1‑275 (MQ=255)
                                                                                                                                                                                          aCGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTg                                                                                                                                                                                                                  <  5:477265/145‑1 (MQ=255)
                                                                                                                                                                                             tgtCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGaa                                                                                                                                                                                                                                                                   <  6:363972/93‑1 (MQ=255)
                                                                                                                                                                                             tgtCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGaa                                                                                                                                                                                                                                                                   >  5:363972/1‑93 (MQ=255)
                                                                                                                                                                                             tgtCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAgcg                                                                                                                                                                                                                              <  5:98154/130‑1 (MQ=255)
                                                                                                                                                                                              gtCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTTTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTgg                                                                              >  2:471891/1‑273 (MQ=255)
                                                                                                                                                                                                cTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTaa                                                                                                                                                                                                                                                              >  7:941793/1‑95 (MQ=255)
                                                                                                                                                                                                   gTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTg                                                                                                                                                                                                                                                                           <  2:96420/79‑1 (MQ=255)
                                                                                                                                                                                                   gTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGTACGGCAGCGCCGCTCCGTTTGTANACCTGCTGCTTTACGCCGGTATCGACGAGTTGCACTTCGACAAAAAATTTGTTCGCATCAAAGAGACTGTTCTGNTCGCAGATGGCGTCAAGTGGTCTGACTTCANNCCTTACAAAGGTTTTTTGCTGTCTTTc                                                                         >  3:70170/1‑273 (MQ=255)
                                                                                                                                                                                                   gTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTc                                                                         >  2:24330/1‑273 (MQ=255)
                                                                                                                                                                                                   gTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCa                                                                        >  2:149039/1‑274 (MQ=255)
                                                                                                                                                                                                    tCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACctg                                                                                                                                                                                                          <  7:1035433/143‑1 (MQ=255)
                                                                                                                                                                                                    tCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACctg                                                                                                                                                                                                          >  8:1035433/1‑143 (MQ=255)
                                                                                                                                                                                                     cAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCa                                                                                                                                                                                                                                          <  6:119522/110‑1 (MQ=255)
                                                                                                                                                                                                      aaCGAGGATGATGTACGGCTTTCAACCGTAGAGCACCTCAAGGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCAt                                                                    <  1:7315/275‑1 (MQ=255)
                                                                                                                                                                                                      aaCGAGCATGATGTCCGGATTTCAACCGTAGAGAACCTCAATGCGTCTCTCGCGGGCTTGGGCTTCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCa                                                                     <  4:24330/274‑1 (MQ=255)
                                                                                                                                                                                                        cGAGCATGATGTACGGATTTCAACCGTAGAGCACCGCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCTGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAAGTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTCCATTGGGTTTTAGCTGGATTTCACCATCg                                                                  >  4:50167/1‑275 (MQ=255)
                                                                                                                                                                                                          aGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGtt                                                                                                                                                                                                                                                                         >  6:1083265/1‑74 (MQ=255)
                                                                                                                                                                                                          aGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGtt                                                                                                                                                                                                                                                                         <  5:1083265/74‑1 (MQ=255)
                                                                                                                                                                                                                   tACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATc                                                                                                                                                                                                                                           <  7:527824/95‑1 (MQ=255)
                                                                                                                                                                                                                   tACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATc                                                                                                                                                                                                                                           >  8:527824/1‑95 (MQ=255)
                                                                                                                                                                                                                   tACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTAAAATGGTTTTTGCCTGGATTTCACCATGGATTTTAACCa                                                        >  4:431346/1‑274 (MQ=255)
                                                                                                                                                                                                                    aCGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcgac                                                                                                                                                                                     >  7:490135/1‑148 (MQ=255)
                                                                                                                                                                                                                    aCGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcgac                                                                                                                                                                                     >  6:1034452/1‑148 (MQ=255)
                                                                                                                                                                                                                      ggATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGa                                                                                                                                                                                                  >  7:482528/1‑133 (MQ=255)
                                                                                                                                                                                                                      ggATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCAATTTTAACCATcc                                                     >  2:168706/1‑274 (MQ=255)
                                                                                                                                                                                                                       gATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATcg                                                                                                                                                                                       <  6:88526/143‑1 (MQ=255)
                                                                                                                                                                                                                       gATTTCAACCGGAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAAcgcg                                                                                                                                                                                                                                                          <  8:573561/76‑1 (MQ=255)
                                                                                                                                                                                                                         tttCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATTATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGtt                                                                                                                                                                                >  5:402594/1‑148 (MQ=255)
                                                                                                                                                                                                                           tCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTa                                                                                                                                                                                                                                                               >  8:277497/1‑67 (MQ=255)
                                                                                                                                                                                                                              aCCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAAc                                                                                                                                                                            >  5:891071/1‑147 (MQ=255)
                                                                                                                                                                                                                              aCCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAATGTTATCGa                                                                                                                                                                                                                                                                    >  6:1121848/1‑59 (MQ=255)
                                                                                                                                                                                                                              aCCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACAATGTTATCGa                                                                                                                                                                                                                                                                    <  5:1121848/59‑1 (MQ=255)
                                                                                                                                                                                                                                   agggaaCCTCAATGCGGCTGTCGCGGGCTTGGGCACCGATAACATTGTTAGCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCa                                       <  4:67663/270‑1 (MQ=255)
                                                                                                                                                                                                                                   agagCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGt                                                                                                                                                                                                                 <  8:720912/105‑1 (MQ=255)
                                                                                                                                                                                                                                   agagCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGt                                                                                                                                                                                                                 >  7:720912/1‑105 (MQ=255)
                                                                                                                                                                                                                                           tCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAAtt                                                                                                                                                              >  6:822436/1‑148 (MQ=255)
                                                                                                                                                                                                                                            cAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAATATGGCGATAAGTGGGCTGAATTTAAGCCGTACACTGGTTTTTCGCTGGATTTCCCCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCa                                >  1:115338/1‑273 (MQ=255)
                                                                                                                                                                                                                                             aaTGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgcg                             <  2:310260/275‑1 (MQ=255)
                                                                                                                                                                                                                                              aTGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACt                                                                                                                                                                           <  8:330539/132‑1 (MQ=255)
                                                                                                                                                                                                                                               tgctgcTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGACTTTCAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCCACCAgcg                             >  2:377807/1‑273 (MQ=255)
                                                                                                                                                                                                                                                 ctgctCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGtt                                                                                                                                                                                                                    <  8:874628/88‑1 (MQ=255)
                                                                                                                                                                                                                                                    ctctCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATg                        >  2:196821/1‑273 (MQ=255)
                                                                                                                                                                                                                                                    ctctCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATg                        >  2:292131/1‑273 (MQ=255)
                                                                                                                                                                                                                                                      ctcGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCg                                                                                                                                                        >  5:64296/1‑143 (MQ=255)
                                                                                                                                                                                                                                                      ctcGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCa                                                                                                                                                   <  6:244642/148‑1 (MQ=255)
                                                                                                                                                                                                                                                        cgcgGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGtata                                                                                                                                                                                                              >  5:136048/1‑87 (MQ=255)
                                                                                                                                                                                                                                                           gggCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATTATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAagag                                                                                                                                              <  6:402594/148‑1 (MQ=255)
                                                                                                                                                                                                                                                           gggCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAagag                                                                                                                                              <  5:822436/148‑1 (MQ=255)
                                                                                                                                                                                                                                                             gCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGAc                                                                                                                                            <  7:656733/148‑1 (MQ=255)
                                                                                                                                                                                                                                                              cTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGtt                                                                                                                                                                                                                    >  8:369056/1‑75 (MQ=255)
                                                                                                                                                                                                                                                                tGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGa                                                                                                                                                                              <  6:598811/111‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTgctgct                                                                                                                                                                                                     <  6:44738/84‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACctgc                                                                                                                                                                                                         >  8:623426/1‑78 (MQ=255)
                                                                                                                                                                                                                                                                      tCGATAACAATGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTAt                                                                                                                                                                                         <  5:1064475/94‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aTAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGtata                                                                                                                                                                                                              <  7:611982/70‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aTAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGc  >  2:347273/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                          tAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGa                                                                                                                                                                                                                                      >  8:488242/1‑45 (MQ=255)
                                                                                                                                                                                                                                                                          tAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGaa                                                                                                                               >  7:393347/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                             cATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGa                                                                                                                                                                              <  5:268626/98‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ttGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAAt                                                                                                                                                               <  6:94916/111‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ttGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGt                                                                                                                                                           <  8:687261/115‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ttGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGt                                                                                                                                                           >  7:687261/1‑115 (MQ=255)
                                                                                                                                                                                                                                                                               ttGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAag                                                                                                                                                <  5:78881/126‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                           
ATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCGCCGGCCAACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAGAGCACCTCAATGCTGCTCTCGCGGGCTTGGGCATCGATAACATTGTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGC  >  NC_000913/106560‑107098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: