Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 765,132 G→A 12.5% intergenic (+83/+21) sucD → / ← mngR succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit/transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913765,1320GA12.5% 108.6 / 8.7 40intergenic (+83/+21)sucD/mngRsuccinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit/transcriptional repressor for the mannosyl‑D‑glycerate catabolic operon
Reads supporting (aligned to +/- strand):  ref base G (19/16);  new base A (3/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-02

ATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAAT  >  NC_000913/764912‑765400
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                            
aTGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTa                                                                                                                                                                                                                        >  4:79516/1‑275 (MQ=255)
       aCGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATCAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACGTGGTTGCCCATCGTATGATGGCCTTTTTTGTTCTTATCGCGATTGTTTTCGCTGCGCTATCAGGGTAAATTtat                                                                                                                                                                                                                 >  4:337828/1‑275 (MQ=255)
        cgcgGGTGCCACCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGCAAACTGTTCTGAAATAAATATCTGTAATACGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTTATTATCGTGCTGATTTTCGCTGCGCTTTCAGGGTAAAttct                                                                                                                                                                                                                  >  1:55232/1‑271 (MQ=255)
        ccgggggaccATCATGGCCGGTGGGAAAGGGACTGCGAATGAGAAATTTGCTGCTCTGGAAACTGCAGGCGTGAAGACCGTTCGCAGCGTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTtata                                                                                                                                                                                                                <  1:67325/267‑1 (MQ=255)
               gcaatatttGCGGGGGGGAAAGGGGNGNGNGAGGAGAATTTGGCTGCTAGGGAAGCCGCAGGCGTGAAAACCGTTNGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCNCCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATc                                                                                                                                                                                                          <  3:54603/267‑1 (MQ=255)
                               ggAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTGTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTg                                                                                                                                                                                          <  2:104360/274‑1 (MQ=255)
                                                                         cAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATACAAACACCCGACCATCAACTAAATATCCAcgc                                                                                                                                                <  2:79517/274‑1 (MQ=255)
                                                                                      cGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGTTATCAGGGTAAATTTATAGTCCTCGGTATTAAACGCGTTGCGGCTATATTCAAACACCCGACCACCAACTAAATAACCAAGCCAGCCttttta                                                                                                                                     >  2:527619/1‑271 (MQ=255)
                                                                                              gCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt                                                                                                                          <  2:543722/275‑1 (MQ=255)
                                                                                                  ggCGGATATCGGCGAAGCACTGAAAACTGTTCTGAAATAAAAATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCGCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGt                                                                                                                      <  4:204906/275‑1 (MQ=255)
                                                                                                                                  tGAAATAAATCTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGTAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatgat                                                                                                                                                                                                                                                                        >  7:374053/1‑97 (MQ=255)
                                                                                                                                  tGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGTCTGTCGGTTTCGACATGGTTGGCCATCGTatgat                                                                                                                                                                                                                                                                        <  8:374053/97‑1 (MQ=255)
                                                                                                                                       taaataTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatga                                                                                                                                                                                                                                                                         <  7:1059151/91‑1 (MQ=255)
                                                                                                                                       taaataTCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTatga                                                                                                                                                                                                                                                                         >  8:1059151/1‑91 (MQ=255)
                                                                                                                                                     aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAGTGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCATACGATGGc                                                                                                                                                                                                                                                                     <  6:145405/81‑1 (MQ=255)
                                                                                                                                                     aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAGTGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCATACGATGGc                                                                                                                                                                                                                                                                     >  5:145405/1‑81 (MQ=255)
                                                                                                                                                     aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTa                                                                                                                                                                                                                                                                            >  7:855440/1‑74 (MQ=255)
                                                                                                                                                     aaGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTa                                                                                                                                                                                                                                                                            <  8:855440/74‑1 (MQ=255)
                                                                                                                                                         aaTAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATCTTCAAACACCCGACCATCAACTAAATCTCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGAAGACGGCTAATCTTTTCGGTTGGCATCAGAGGAATGAtt                                                               >  1:103786/1‑275 (MQ=255)
                                                                                                                                                           tAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTgcg                                                                                                                                                                                                                                   >  4:17319/1‑109 (MQ=255)
                                                                                                                                                           tAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGAttt                                                              >  2:321847/1‑274 (MQ=255)
                                                                                                                                                               ccTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTAt                                                                                                                                                                                                                               <  2:17319/109‑1 (MQ=255)
                                                                                                                                                                        ttCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgtg                                                                                                                                                                                                                                                           >  7:88230/1‑72 (MQ=255)
                                                                                                                                                                        ttCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTtgtg                                                                                                                                                                                                                                                           <  8:88230/72‑1 (MQ=255)
                                                                                                                                                                        ttCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAg                                                                                                    >  2:206124/1‑223 (MQ=255)
                                                                                                                                                                            ctctACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACgg                                                                                                <  4:206123/223‑1 (MQ=255)
                                                                                                                                                                                         gCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTttctt                               >  2:317223/1‑275 (MQ=255)
                                                                                                                                                                                                   gTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGAc                                                                                                                                                                    >  7:131546/1‑132 (MQ=255)
                                                                                                                                                                                                   gTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGAc                                                                                                                                                                    <  8:131546/132‑1 (MQ=255)
                                                                                                                                                                                                      ggTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTtat                                                                                                                                                                                                                 <  6:586759/84‑1 (MQ=255)
                                                                                                                                                                                                      ggTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTtat                                                                                                                                                                                                                 >  5:586759/1‑84 (MQ=255)
                                                                                                                                                                                                        tttCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTgcgc                                                                                                                                                                                                                                  <  7:10902/65‑1 (MQ=255)
                                                                                                                                                                                                        tttCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTgcgc                                                                                                                                                                                                                                  >  8:10902/1‑65 (MQ=255)
                                                                                                                                                                                                         ttCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGCCTCATCTCTTCGGTTGGCATCCAAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAg               >  2:319381/1‑275 (MQ=255)
                                                                                                                                                                                                                       ccATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCCACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCCGAGGAATGATTTTCTGTTCGCTTCGATCGAATACCATTTTCTTCCCTTCTTCACTAAAGTGCTTTTTCGAAt  >  4:294397/1‑274 (MQ=255)
                                                                                                                                                                                                                       ccATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGc                                                                                                                                                                                      <  6:113980/94‑1 (MQ=255)
                                                                                                                                                                                                                       ccATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGc                                                                                                                                                                                      >  5:113980/1‑94 (MQ=255)
                                                                                                                                                                                                                       ccATCATACGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGaa                                                                                                                                >  5:908163/1‑148 (MQ=255)
                                                                                                                                                                                                                        cATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAcg                                                                                                                                                 >  8:703307/1‑130 (MQ=255)
                                                                                                                                                                                                                        cATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCAcg                                                                                                                                                 <  7:703307/130‑1 (MQ=255)
                                                                                                                                                                                                                            gTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt                                                                                                                          >  4:551340/1‑149 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                            
ATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGATTTCCTGTTCGCTACGATCGATAACCATTTTCTTCACTTCTTCGATAAAGTGATATTTCGAAT  >  NC_000913/764912‑765400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑