Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,729,413 Δ1 bp 6.5% intergenic (‑42/+30) xylB ← / ← xylA xylulokinase/D‑xylose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,729,4130A. 6.5% 672.7 / 38.3 184intergenic (‑42/+30)xylB/xylAxylulokinase/D‑xylose isomerase
Reads supporting (aligned to +/- strand):  ref base A (89/83);  new base . (7/5);  total (96/88)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 189 total aligned reads displayed.

GGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACAT  >  NC_000913/3729266‑3729559
                                                                                                                                                    |                                                                                                                                                  
ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATa                                                                                                                                                    >  2:52895/1‑148 (MQ=255)
    cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCg                                                                                                                                                <  1:52895/148‑1 (MQ=255)
               gCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCg                                                                                                                                                >  4:1053698/1‑137 (MQ=255)
                  tttCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGc                                                                                                                                  <  4:384887/148‑1 (MQ=255)
                        tttGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATaccg                                                                                                                                                 >  2:336683/1‑125 (MQ=255)
                        tttGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATaccg                                                                                                                                                 <  1:336683/128‑4 (MQ=255)
                                   accaccGCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAttt                                                                                                                 <  3:165674/148‑1 (MQ=255)
                                    ccaccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTg                                                                                                                >  4:179888/1‑148 (MQ=255)
                                         tCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGc                                                                                                                                             <  3:1074966/114‑1 (MQ=255)
                                                                    tttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGa                                                                                                                                      >  4:729582/1‑94 (MQ=255)
                                                                    tttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACt                                                                                                                                    <  2:532705/96‑1 (MQ=255)
                                                                    tttACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACt                                                                                                                                    >  1:532705/1‑96 (MQ=255)
                                                                      tACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGa                                                                                                                                      <  1:428590/92‑1 (MQ=255)
                                                                      tACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGa                                                                                                                                      >  2:428590/1‑92 (MQ=255)
                                                                          cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGcaca                                                                                                                               <  1:1075610/95‑1 (MQ=255)
                                                                          cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAtttt                                                                                    >  3:1040795/1‑138 (MQ=255)
                                                                          cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTc                                                                                   >  1:741747/1‑139 (MQ=255)
                                                                          cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTc                                                                                   <  2:741747/139‑1 (MQ=255)
                                                                                    cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAAcc                                                                                                                                                 >  2:16160/1‑67 (MQ=255)
                                                                                    cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAAcc                                                                                                                                                 <  1:16160/67‑1 (MQ=255)
                                                                                    cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCaa                                                                                                                                          <  1:698043/74‑1 (MQ=255)
                                                                                    cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCaa                                                                                                                                          >  2:698043/1‑74 (MQ=255)
                                                                                    cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                                                                                  >  2:1053443/1‑98 (MQ=255)
                                                                                    cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGa                                                                   >  3:708755/1‑145 (MQ=255)
                                                                                      aGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGtt                                                                                                                     >  4:74609/1‑93 (MQ=255)
                                                                                      aGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGtt                                                                                                                     <  3:74609/93‑1 (MQ=255)
                                                                                      aGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                                                                               >  3:923314/1‑115 (MQ=255)
                                                                                      aGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                                                                               <  4:923314/115‑1 (MQ=255)
                                                                                           tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAAt                                                                                                   >  1:294196/1‑106 (MQ=255)
                                                                                           tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAAt                                                                                                   <  2:294196/106‑1 (MQ=255)
                                                                                             tCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                                                                                  >  2:840158/1‑89 (MQ=255)
                                                                                             tCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGa                                                                                                            >  3:514685/1‑95 (MQ=255)
                                                                                             tCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGa                                                                                                            <  4:514685/95‑1 (MQ=255)
                                                                                              cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                                                                                                        >  3:479328/1‑98 (MQ=255)
                                                                                              cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCa                                                      >  1:656428/1‑148 (MQ=255)
                                                                                               ccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGa                                                                                        <  4:477200/113‑1 (MQ=255)
                                                                                                    ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgag                                                <  2:656428/148‑1 (MQ=255)
                                                                                                      aCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                                                                                                        <  4:1101304/90‑1 (MQ=255)
                                                                                                      aCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTc                                                                          >  4:781936/1‑120 (MQ=255)
                                                                                                         tatCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                           >  1:861965/1‑148 (MQ=255)
                                                                                                           tcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGtt                                                                                                                     >  3:326779/1‑72 (MQ=255)
                                                                                                           tcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGt                                                                                                <  3:1023182/93‑1 (MQ=255)
                                                                                                           tcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTa                                                                                             >  1:666413/1‑96 (MQ=255)
                                                                                                           tcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTa                                                                                             <  2:666413/96‑1 (MQ=255)
                                                                                                            cgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                                                                           <  3:802352/97‑1 (MQ=255)
                                                                                                            cgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg                                                           >  1:724913/1‑129 (MQ=255)
                                                                                                            cgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg                                                           <  2:724913/129‑1 (MQ=255)
                                                                                                              atcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAg                                                                                         <  1:240467/97‑1 (MQ=255)
                                                                                                              atcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAg                                                                                         >  2:240467/1‑97 (MQ=255)
                                                                                                              atcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTc                                                                                   <  3:197215/103‑1 (MQ=255)
                                                                                                               tcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTa                                                                                             <  1:407804/92‑1 (MQ=255)
                                                                                                               tcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTa                                                                                             >  2:407804/1‑92 (MQ=255)
                                                                                                               tcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCgg                                                  <  2:215593/135‑1 (MQ=255)
                                                                                                               tcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCgg                                                  >  1:215593/1‑135 (MQ=255)
                                                                                                                   tCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGcaca                                                                                                                               >  4:339043/1‑54 (MQ=255)
                                                                                                                   tCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                           >  3:549109/1‑138 (MQ=255)
                                                                                                                   tCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                           <  4:549109/138‑1 (MQ=255)
                                                                                                                      ttAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCg                                                                    <  4:484187/110‑1 (MQ=255)
                                                                                                                       tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaa                                            >  4:244146/1‑133 (MQ=255)
                                                                                                                       tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGCCGAACAGATAATGGTTTACCAGAtttt                                                                                    >  2:576396/1‑93 (MQ=255)
                                                                                                                       tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGCCGAACAGATAATGGTTTACCAGAtttt                                                                                    <  1:576396/93‑1 (MQ=255)
                                                                                                                       tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCAAACTTAGCCGTTATTTGTCTAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGgaga                                               <  2:808424/130‑1 (MQ=255)
                                                                                                                          aaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGt                                                                                                               >  4:494484/1‑63 (MQ=255)
                                                                                                                          aaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGt                                                                                                               <  3:494484/63‑1 (MQ=255)
                                                                                                                          aaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCAc                                                               <  3:1061620/111‑1 (MQ=255)
                                                                                                                          aaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCgg                                                  >  3:784370/1‑124 (MQ=255)
                                                                                                                           aaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTa                                                                                                                    >  3:244544/1‑57 (MQ=255)
                                                                                                                           aaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTg                                                                                                                >  2:1058969/1‑61 (MQ=255)
                                                                                                                           aaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATg                                                                                                  >  2:831510/1‑75 (MQ=255)
                                                                                                                            aaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGa                                                                   >  1:301274/1‑105 (MQ=255)
                                                                                                                             aaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                           <  4:1072859/128‑1 (MQ=255)
                                                                                                                              aTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTg                      <  3:432069/148‑1 (MQ=255)
                                                                                                                                  ccGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTa                                                                                                                           <  1:320049/43‑1 (MQ=255)
                                                                                                                                   cggttATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGa                                                          >  1:835467/5‑107 (MQ=255)
                                                                                                                                   cggttATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa                 >  3:730918/5‑148 (MQ=255)
                                                                                                                                   cggttATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa                 >  2:1014503/5‑148 (MQ=255)
                                                                                                                                   cggttATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAACGATGTTCCTGAGCATATTTGGCTaa                 >  1:808492/5‑148 (MQ=255)
                                                                                                                                   cGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTcc                                                                                  <  1:10556/83‑1 (MQ=255)
                                                                                                                                   cGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCActct                                                            >  4:1037514/1‑105 (MQ=255)
                                                                                                                                    ggTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTgg                                                                      <  2:304394/94‑1 (MQ=255)
                                                                                                                                       tATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGc                                                       <  1:1044560/107‑1 (MQ=255)
                                                                                                                                       tATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTgg                     <  4:1017351/141‑1 (MQ=255)
                                                                                                                                       tATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTgg                     >  3:1017351/1‑141 (MQ=255)
                                                                                                                                        aTCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTg            <  4:79387/148‑1 (MQ=255)
                                                                                                                                          cGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATAtt                        >  4:401084/1‑135 (MQ=255)
                                                                                                                                            cTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAg                                                                                         >  2:945318/1‑68 (MQ=255)
                                                                                                                                             tACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTcc                                                                                  <  2:1041321/74‑1 (MQ=255)
                                                                                                                                             tACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTcc                                                                                  >  1:1041321/1‑74 (MQ=255)
                                                                                                                                             tACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTcc                                  <  3:778743/122‑1 (MQ=255)
                                                                                                                                              aCCGATAACCGGGCCAACGGACCGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGt       >  4:824905/1‑148 (MQ=255)
                                                                                                                                               ccGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatg                                         <  2:1159/113‑1 (MQ=255)
                                                                                                                                               ccGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatg                                         >  1:1159/1‑113 (MQ=255)
                                                                                                                                                cGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACaaa                                           >  4:319233/1‑110 (MQ=255)
                                                                                                                                                 gATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCa                                                      >  3:342744/1‑98 (MQ=255)
                                                                                                                                                 gATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGAc    >  2:1105367/1‑148 (MQ=255)
                                                                                                                                                  aTAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTg                      >  1:152010/1‑129 (MQ=255)
                                                                                                                                                  aTAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTg                      <  2:152010/129‑1 (MQ=255)
                                                                                                                                                   tAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatgat                                       <  1:789337/111‑1 (MQ=255)
                                                                                                                                                   tAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAg        >  3:398320/1‑142 (MQ=255)
                                                                                                                                                   tAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACAt  >  4:157882/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACAT  >  NC_000913/3729266‑3729559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: