| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,463,481 | Δ1 bp | 13.8% | intergenic (+43/‑58) | paaK → / → paaX | phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,463,481 | 0 | G | . | 13.8% | 207.8 / 30.5 | 65 | intergenic (+43/‑58) | paaK/paaX | phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer |
| Reads supporting (aligned to +/- strand): ref base G (27/29); new base . (4/5); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
TCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGTATGGTGATTCG > NC_000913/1463341‑1463628 | tctcTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatac < 2:973960‑M2/148‑7 (MQ=255) tctACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGa < 1:1008694/148‑1 (MQ=255) atCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTc < 1:973960/148‑1 (MQ=255) ggCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATg < 4:496530/132‑1 (MQ=255) ggCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATg > 3:496530/1‑132 (MQ=255) cAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCAcaacaa > 1:409625/1‑148 (MQ=255) aGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAAt > 1:1043294/1‑148 (MQ=255) aaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATg < 2:1043294/148‑1 (MQ=255) tgtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTaaa < 2:409625/148‑1 (MQ=255) cGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAg < 1:171501/79‑1 (MQ=255) cGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAg > 2:171501/1‑79 (MQ=255) gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAg > 2:447631/1‑110 (MQ=255) gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAg < 1:447631/110‑1 (MQ=255) tGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggta < 2:396354‑M2/148‑102 (MQ=255) gACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggata > 3:343372‑M2/1‑46 (MQ=255) gACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggata < 4:343372‑M2/117‑72 (MQ=255) aCGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa > 2:1061698/1‑101 (MQ=255) aCGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa < 1:1061698/101‑1 (MQ=255) tCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa < 3:1130786/77‑1 (MQ=255) tCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa < 3:1056002/77‑1 (MQ=255) tCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa > 4:1130786/1‑77 (MQ=255) tCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa > 4:1056002/1‑77 (MQ=255) cTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 1:159422/110‑1 (MQ=255) cTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 2:159422/1‑110 (MQ=255) tGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcgcatcggtagagatccccaccatc < 2:1002090‑M2/148‑116 (MQ=255) ggCCCTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaa < 3:532854‑M2/115‑84 (MQ=255) ggCCCTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaa > 4:532854‑M2/1‑32 (MQ=255) gCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAc < 4:523393/106‑1 (MQ=255) gCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAc > 3:523393/1‑106 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatccccaccatcga > 1:396354‑M2/1‑30 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 1:974691/104‑1 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 2:974691/1‑104 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATc > 1:657789/1‑148 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatcc > 1:962068‑M2/1‑28 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatcc < 2:962068‑M2/135‑108 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaa > 1:468484‑M2/1‑28 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaa < 2:468484‑M2/63‑36 (MQ=255) tggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa < 2:690522/123‑1 (MQ=255) tggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa > 1:690522/1‑123 (MQ=255) ggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCa < 4:702487/110‑1 (MQ=255) ggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCa > 3:702487/1‑110 (MQ=255) ggtggGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatccccaccatcgacttaa < 4:187228‑M2/148‑123 (MQ=255) gtggGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatccccaccatcgacttaat > 3:187228‑M2/1‑25 (MQ=255) ggggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGa < 2:657789/148‑1 (MQ=255) gTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTcc > 2:949017/1‑125 (MQ=255) gTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTcc < 1:949017/125‑1 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACt < 2:474432/88‑1 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACt > 1:474432/1‑88 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg < 4:961670/130‑1 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg > 3:961670/1‑130 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAtctc > 1:291769/1‑148 (MQ=255) aaaGCGCCAGGGCTAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa > 3:77455/1‑114 (MQ=255) aaaGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa < 4:77455/114‑1 (MQ=255) aGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGa < 4:112128/69‑1 (MQ=255) aGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGa > 3:112128/1‑69 (MQ=255) gcgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACCGACTGAAGCGAATGAGTAAACTTg < 2:417160/86‑1 (MQ=255) gcgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTg > 1:417160/1‑86 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 2:302089/90‑1 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 4:355096/90‑1 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 1:302089/1‑90 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 3:355096/1‑90 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTa < 3:788131/94‑1 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTa > 4:788131/1‑94 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaa > 2:108851/1‑109 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaa < 1:108851/109‑1 (MQ=255) gAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 4:1118803/1‑79 (MQ=255) gAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 3:1118803/79‑1 (MQ=255) gTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGTATGGTGATTCg < 2:291769/148‑1 (MQ=255) gTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGTATGGTGATTCg > 4:465667/1‑148 (MQ=255) | TCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGTATGGTGATTCG > NC_000913/1463341‑1463628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |