Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,555 Δ1 bp 5.4% coding (1126/1452 nt) uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,5550G. 5.4% 474.1 / 19.5 130coding (1126/1452 nt)uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (62/61);  new base . (4/3);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 135 total aligned reads displayed.

GAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAG  >  NC_000913/1609409‑1609696
                                                                                                                                                  |                                                                                                                                              
      cTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgt                                                                                                                                        >  2:78818/1‑148 (MQ=255)
         aTAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                                                                     >  3:611215/1‑148 (MQ=255)
         aTAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                                                                     >  2:705883/1‑148 (MQ=255)
         aTAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                                                                     >  1:445078/1‑148 (MQ=255)
            aCGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAgg                                                                                                                                  >  4:727212/1‑148 (MQ=255)
               ttCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATg                                                                                                                               >  1:546851/1‑148 (MQ=255)
                tCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGc                                                                                                                              >  4:275235/1‑148 (MQ=255)
                tCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAg                                                                                                                                   <  3:598045/143‑1 (MQ=255)
                tCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAg                                                                                                                                   >  4:598045/1‑143 (MQ=255)
                 ccagccagGGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCg                                                                                                                             <  1:705883/148‑1 (MQ=255)
                  cagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCgg                                                                                                                            <  2:546851/148‑1 (MQ=255)
                    gccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                                                          >  1:420697/1‑133 (MQ=255)
                    gccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                                                          >  3:16966/1‑133 (MQ=255)
                     ccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTg                                                                                                                         >  4:35876/1‑148 (MQ=255)
                      cagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGc                                                                                                                        >  1:143858/1‑148 (MQ=255)
                         tgtgCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                                                                     >  2:298344/1‑132 (MQ=255)
                             cATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                                                                 <  1:377277/148‑1 (MQ=255)
                             cATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                                                                 <  3:275235/148‑1 (MQ=255)
                              aTCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTg                                                                                                                >  4:1035901/1‑148 (MQ=255)
                                   ccTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                                                            >  2:38960/1‑116 (MQ=255)
                                   ccTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                                                            <  1:38960/116‑1 (MQ=255)
                                    cTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATa                                                                                                          >  1:467113/1‑148 (MQ=255)
                                        aCCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACt                                                                                                                  >  4:55608/1‑136 (MQ=255)
                                        aCCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACt                                                                                                                  <  3:55608/136‑1 (MQ=255)
                                          cGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                                                                    <  2:445078/148‑1 (MQ=255)
                                             atatGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGtt                                                                                                     <  4:783341/144‑1 (MQ=255)
                                             atatGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGt                                                                                                      <  1:733031/143‑1 (MQ=255)
                                             atatGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGt                                                                                                      >  2:733031/1‑143 (MQ=255)
                                              tatGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGt                                                                                                      <  1:197910/142‑1 (MQ=255)
                                              tatGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGt                                                                                                      >  2:197910/1‑142 (MQ=255)
                                               atGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagcag                                                                                                                                             <  3:321743/103‑1 (MQ=255)
                                               atGTTTCGCCGTTAAGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagcag                                                                                                                                             >  4:321743/1‑103 (MQ=255)
                                                tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                                                                    <  1:231859/142‑1 (MQ=255)
                                                tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                                                                    <  1:674979/142‑1 (MQ=255)
                                                tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                                                              <  3:727212/148‑1 (MQ=255)
                                                tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                                                              <  2:143858/148‑1 (MQ=255)
                                                 gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa                                                                                             >  4:149645/1‑148 (MQ=255)
                                                 gTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa                                                                                             <  1:398433/148‑1 (MQ=255)
                                                  tttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                                                            <  2:901233/148‑1 (MQ=255)
                                                   ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                                                              >  3:1145513/1‑145 (MQ=255)
                                                   ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                                                              <  4:1145513/145‑1 (MQ=255)
                                                   ttCGCCGCTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATa                                                                                                          >  2:664308/1‑133 (MQ=255)
                                                     cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                                                            >  1:924115/1‑145 (MQ=255)
                                                     cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                                                            <  2:924115/145‑1 (MQ=255)
                                                       ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                                                               >  4:909840/1‑124 (MQ=255)
                                                       ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                                                                 >  4:1091617/1‑105 (MQ=255)
                                                       ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                                                                 <  3:1091617/106‑2 (MQ=255)
                                                       ccGTGACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                                                               <  3:909840/124‑1 (MQ=255)
                                                          ttACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                                                              <  3:281889/122‑1 (MQ=255)
                                                                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCa                                                                                                                                    <  1:484867/89‑1 (MQ=255)
                                                                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCa                                                                                                                                    >  2:484867/1‑89 (MQ=255)
                                                                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTg                                                                                                                <  2:392010/109‑1 (MQ=255)
                                                                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                                                              <  1:913832/111‑1 (MQ=255)
                                                                         tAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCAt                                                                                                           <  1:404949/110‑1 (MQ=255)
                                                                         tAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCAt                                                                                                           >  2:404949/1‑110 (MQ=255)
                                                                           gAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTg                                                                                                                <  3:45476/103‑1 (MQ=255)
                                                                           gAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTg                                                                                                                >  4:45476/1‑103 (MQ=255)
                                                                                  atcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa                                                                                             <  3:1133323/115‑1 (MQ=255)
                                                                                  atcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa                                                                                             >  4:1133323/1‑115 (MQ=255)
                                                                                  atcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTAc                                                            >  1:844658/1‑148 (MQ=255)
                                                                                   tcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                                                                 <  2:412323/94‑1 (MQ=255)
                                                                                   tcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                                                           >  2:456931/1‑148 (MQ=255)
                                                                                    caatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAg                                                                                                                                   >  3:379778/1‑75 (MQ=255)
                                                                                           gCTAATTCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                                                              >  2:608157/1‑89 (MQ=255)
                                                                                           gCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                                                              <  1:608157/89‑1 (MQ=255)
                                                                                             tAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc                                                   >  1:899124/1‑146 (MQ=255)
                                                                                             tAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc                                                   <  2:899124/146‑1 (MQ=255)
                                                                                             tAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc                                                   >  1:826470/1‑146 (MQ=255)
                                                                                              aaTGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                                                             >  1:1116656/1‑119 (MQ=255)
                                                                                                  cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                                                           >  4:473833/1‑117 (MQ=255)
                                                                                                    aaaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTa                                                                         >  1:285105/1‑117 (MQ=255)
                                                                                                    aaaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTa                                                                         <  2:285105/117‑1 (MQ=255)
                                                                                                     aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTa                                                                    >  3:179076/1‑121 (MQ=255)
                                                                                                     aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTa                                                                    <  4:179076/121‑1 (MQ=255)
                                                                                                         aaGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc                                       >  4:909722/1‑146 (MQ=255)
                                                                                                         aaGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc                                       <  3:909722/146‑1 (MQ=255)
                                                                                                                 ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAc                                                                                        >  3:873607/1‑89 (MQ=255)
                                                                                                                 ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGa                             <  2:844658/148‑1 (MQ=255)
                                                                                                                    gAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagcag                                                                                   <  4:280658/91‑1 (MQ=255)
                                                                                                                       gtgtGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAg                       <  3:1035901/148‑1 (MQ=255)
                                                                                                                             cGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGaa                                <  3:347647/133‑1 (MQ=255)
                                                                                                                             cGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGaa                                >  4:347647/1‑133 (MQ=255)
                                                                                                                                ttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagc                                                                                     >  1:39238/1‑77 (MQ=255)
                                                                                                                                ttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagc                                                                                     <  2:39238/77‑1 (MQ=255)
                                                                                                                                ttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACg                                                                  >  2:436342/1‑96 (MQ=255)
                                                                                                                                ttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACg                                                                  <  1:436342/96‑1 (MQ=255)
                                                                                                                                ttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg                                                >  4:572019/1‑114 (MQ=255)
                                                                                                                                      tCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACg              >  1:1062048/1‑142 (MQ=255)
                                                                                                                                      tCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACg              <  2:1062048/142‑1 (MQ=255)
                                                                                                                                          tcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATcc    >  3:44138/2‑148 (MQ=255)
                                                                                                                                           cctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTcc                         >  3:534613/1‑126 (MQ=255)
                                                                                                                                           cctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGaa                                >  4:504748/1‑119 (MQ=255)
                                                                                                                                           cctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGaa                                <  3:504748/119‑1 (MQ=255)
                                                                                                                                            ctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg  >  4:868008/1‑148 (MQ=255)
                                                                                                                                            ctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg  >  2:527601/1‑148 (MQ=255)
                                                                                                                                             tgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAAt      <  1:501377/143‑1 (MQ=255)
                                                                                                                                             tgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAAt      >  2:501377/1‑143 (MQ=255)
                                                                                                                                              gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                                                           >  2:147561/1‑73 (MQ=255)
                                                                                                                                              gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGt                      >  1:313511/1‑126 (MQ=255)
                                                                                                                                              gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGt                      <  2:313511/126‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAG  >  NC_000913/1609409‑1609696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: