Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,092,779 Δ1 bp 5.2% coding (902/951 nt) gshB → glutathione synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,092,7790T. 5.2% 557.9 / 21.4 153coding (902/951 nt)gshBglutathione synthetase
Reads supporting (aligned to +/- strand):  ref base T (73/72);  new base . (4/4);  total (77/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

AAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAG  >  NC_000913/3092634‑3092925
                                                                                                                                                  |                                                                                                                                                  
aaaaTCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCa                                                                                                                                                  >  3:992178/1‑148 (MQ=255)
  aaTCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCAcc                                                                                                                                                <  1:605710/148‑1 (MQ=255)
                tCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGa                                                                                                                                  <  4:992178/148‑1 (MQ=255)
                           cTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCa                                                                                                                                                  >  1:76715/1‑121 (MQ=255)
                                agaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGt                                                                                                                  >  4:1085470/1‑148 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATc                                                                                                                                                   <  2:661334/114‑1 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTa                                                                                                                                       <  2:590338/126‑1 (MQ=255)
                                         gCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGCGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGaa                                                                                                                                            <  1:230481/113‑1 (MQ=255)
                                         gCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGaa                                                                                                                                            >  2:230481/1‑113 (MQ=255)
                                          cTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCGGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                                        >  1:394304/1‑148 (MQ=255)
                                          cTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTa                                                                                                               >  1:835871/1‑141 (MQ=255)
                                           tGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                                       >  1:1043443/1‑148 (MQ=255)
                                            gATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGa                                                                                                                                  <  1:675509/120‑1 (MQ=255)
                                                     tgGTCTGGATATCATCAGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGa                                                                                                                                             >  4:1074456/1‑100 (MQ=255)
                                                     tgGTCTGGATATCATCAGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGa                                                                                                                                             <  3:1074456/100‑1 (MQ=255)
                                                        tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAAcc                                                                                                 >  2:759334/1‑141 (MQ=255)
                                                        tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTa                                                                                          >  2:1094218/1‑148 (MQ=255)
                                                         cTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGa                                                                                                                         <  2:785273/116‑1 (MQ=255)
                                                         cTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                                                     >  2:744801/1‑120 (MQ=255)
                                                         cTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                                                     <  1:744801/120‑1 (MQ=255)
                                                           ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                                                      <  4:150651/101‑1 (MQ=255)
                                                           ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                                                      >  3:150651/1‑101 (MQ=255)
                                                            gATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga                                                                                      >  2:1062012/1‑148 (MQ=255)
                                                               atcatcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                                                   >  4:652422/1‑148 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                                                     >  4:323362/1‑110 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                                                     >  2:811605/1‑110 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                                                     <  1:811605/110‑1 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                                                     <  3:323362/110‑1 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                                        >  4:890185/1‑123 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAt                                                                               >  1:902654/1‑148 (MQ=255)
                                                                    cGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATTGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtc                                                                              >  1:194078/1‑148 (MQ=255)
                                                                       cGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagc                                                                                                           >  3:780116/1‑116 (MQ=255)
                                                                       cGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagc                                                                                                           <  4:780116/116‑1 (MQ=255)
                                                                        gACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGTAGGATCTCGt                                                                          <  3:652422/148‑1 (MQ=255)
                                                                         aCCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                                                      >  3:739417/1‑87 (MQ=255)
                                                                         aCCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTa                                                                                                               <  4:534772/110‑1 (MQ=255)
                                                                         aCCGTCTGACTGAAATTAACGCCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCAGAATGTTaa                                                                                                                                      <  4:739417/87‑1 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGt                                                                                                                  <  2:1045062/102‑1 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                                        <  3:1088729/112‑1 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCtt                                                                                           >  3:1063224/1‑125 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagca                                                                                                          >  4:336553/1‑104 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                                                   >  3:10054/1‑127 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                                                   <  4:10054/127‑1 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                                          <  1:217267/136‑1 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTg                                                                        >  4:21283/1‑138 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTg                                                                        <  3:21283/138‑1 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAg                                                              >  2:495791/1‑148 (MQ=255)
                                                                                      aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                                         <  2:384644/119‑1 (MQ=255)
                                                                                      aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                                         >  1:384644/1‑119 (MQ=255)
                                                                                        ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                          <  1:495791/148‑1 (MQ=255)
                                                                                         tAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAc                                                                                                              >  3:843717/1‑95 (MQ=255)
                                                                                         tAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAc                                                                                                              <  2:233326/95‑1 (MQ=255)
                                                                                         tAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCAACGAAGCACGTTTAc                                                                                                              <  4:843717/95‑1 (MQ=255)
                                                                                            cGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                                >  4:1055971/1‑122 (MQ=255)
                                                                                            cGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAgcg                                                      <  1:1062012/148‑1 (MQ=255)
                                                                                                aCCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                               >  1:875847/1‑135 (MQ=255)
                                                                                                aCCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                               <  2:875847/135‑1 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGc                                                                                                                               <  4:548891/70‑1 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGc                                                                                                                               >  3:548891/1‑70 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                                >  1:244535/1‑117 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                               <  4:703150/134‑1 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                               >  3:703150/1‑134 (MQ=255)
                                                                                                  cAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                                                      <  3:82063/62‑1 (MQ=255)
                                                                                                  cAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAg                                                              <  2:315541/134‑1 (MQ=255)
                                                                                                   aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCtt                                                                                           <  4:417926/104‑1 (MQ=255)
                                                                                                      ccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                                                   >  1:1083056/1‑109 (MQ=255)
                                                                                                        aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                                          >  4:1095136/1‑116 (MQ=255)
                                                                                                        aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                                          <  3:1095136/116‑1 (MQ=255)
                                                                                                            tGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTg                                                                        <  1:559421/114‑1 (MQ=255)
                                                                                                               aTTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                                          <  2:285339/141‑1 (MQ=255)
                                                                                                               aTTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                                          >  1:285339/1‑141 (MQ=255)
                                                                                                                 tCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTa                                                                                                    <  1:325952/81‑1 (MQ=255)
                                                                                                                         ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAAccc                                                                                                <  1:989162/77‑1 (MQ=255)
                                                                                                                          tGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGGGTGACAGCGCCCTTCTTTCCACGCATACTg                                 >  2:970984/1‑139 (MQ=255)
                                                                                                                               cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                                                   <  3:63996/84‑1 (MQ=255)
                                                                                                                               cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                                                   >  4:63996/1‑84 (MQ=255)
                                                                                                                               cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                               <  1:191159/104‑1 (MQ=255)
                                                                                                                               cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                               >  2:191159/1‑104 (MQ=255)
                                                                                                                                taacattCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt                         >  4:19341/6‑141 (MQ=255)
                                                                                                                                taacattCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt                         <  3:19341/136‑1 (MQ=255)
                                                                                                                                agagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                                         >  4:791386/1‑77 (MQ=255)
                                                                                                                                agagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTg                                                            <  2:350809/106‑1 (MQ=255)
                                                                                                                                   gTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATAc                                   >  4:280412/1‑128 (MQ=255)
                                                                                                                                   gTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATAc                                   <  3:280412/128‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                                          >  1:632680/1‑87 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                                          <  2:632680/87‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg                                 <  2:395377/128‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg                                 <  3:975966/128‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgcgc                            <  3:36070/133‑1 (MQ=255)
                                                                                                                                      tCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                                          <  2:859119/86‑1 (MQ=255)
                                                                                                                                      tCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTg                                                                <  2:204039/96‑1 (MQ=255)
                                                                                                                                      tCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt                         <  3:537708/135‑1 (MQ=255)
                                                                                                                                           tgtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                          <  4:411801/97‑1 (MQ=255)
                                                                                                                                           tgtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                          >  3:411801/1‑97 (MQ=255)
                                                                                                                                            gtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg                                 >  4:468203/1‑121 (MQ=255)
                                                                                                                                            gtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg                                 <  3:468203/121‑1 (MQ=255)
                                                                                                                                                 aTCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgcgc                            >  2:986156/1‑122 (MQ=255)
                                                                                                                                                 aTCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgcgc                            <  1:986156/122‑1 (MQ=255)
                                                                                                                                                 aTCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg  >  1:1011908/1‑148 (MQ=255)
                                                                                                                                                  tCACCCGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCcttc                                                <  3:454266/101‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAG  >  NC_000913/3092634‑3092925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: