Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,100,126:1 +A 5.9% coding (487/555 nt) ycdY → redox enzyme maturation protein (REMP) chaperone for YcdX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,100,1261.A 5.9% 752.5 / 41.3 169coding (487/555 nt)ycdYredox enzyme maturation protein (REMP) chaperone for YcdX
Reads supporting (aligned to +/- strand):  ref base . (77/82);  new base A (5/5);  total (82/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCG  >  NC_000913/1099986‑1100256
                                                                                                                                             |                                                                                                                                  
        tggctggAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAccc                                                                                                                     >  1:677267/1‑148 (MQ=255)
        tagcagaaaacgcggccatgaatgagatcgagcacacattttatgcaacaacaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACCATGGCACCGCTAAccc                                                                                                                     <  1:145924‑M1/21‑1 (MQ=255)
         ggctggAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCcg                                                                                                                    >  4:562754/1‑148 (MQ=255)
               aaGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGc                                                                                                              >  2:602403/1‑148 (MQ=255)
               aaGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGc                                                                                                              >  3:557585/1‑148 (MQ=255)
                aGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGcc                                                                                                             <  1:1077363/148‑1 (MQ=255)
                  aTCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAt                                                                                                           >  4:960116/1‑148 (MQ=255)
                   tCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                                                          <  3:760361/148‑1 (MQ=255)
                   tCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                                                          <  3:960116/148‑1 (MQ=255)
                      gTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCa                                                                                                                                     <  3:305883/119‑1 (MQ=255)
                        cAACGGAAGATGAGAGCGAAGCACTGGAAACAGTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGc                                                                                                     >  3:721171/1‑148 (MQ=255)
                        cAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAg                                                                                                        <  2:282828/145‑1 (MQ=255)
                        cAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAg                                                                                                        >  1:282828/1‑145 (MQ=255)
                           cGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAAt                                                                                                  >  1:230922/1‑148 (MQ=255)
                                  atcgagcacacattttatgcaacaacaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTAATGGCACCGCTAACCCGCGATg                                                                                                               <  1:668743‑M1/28‑1 (MQ=255)
                                   gagaGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTggg                                                                                             <  1:398412/145‑1 (MQ=255)
                                   gagaGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTggg                                                                                             >  2:398412/1‑145 (MQ=255)
                                    agagCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGa                                                                                         >  1:395004/1‑148 (MQ=255)
                                          cacattttatgcaacaacaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTAATGGCACCGCTAACCCGCGATGCCATTAg                                                                                                        >  4:1129154‑M1/94‑128 (MQ=255)
                                          cacattttatgcaacaacaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTAATGGCACCGCTAACCCGCGATGCCATTAg                                                                                                        <  3:1129154‑M1/35‑1 (MQ=255)
                                              ttttatgcaacaacaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACCATGGCACCGCTAACCCGCGATGc                                                                                                              >  3:351795‑M1/90‑117 (MQ=255)
                                               tttatgcaacaacaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTAATGGCACCGCTAACCCGCGATg                                                                                                               <  4:676895‑M1/28‑1 (MQ=255)
                                                  gAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCa                                                                                                                               >  3:244235/1‑96 (MQ=255)
                                                   aaaCACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGga                                                                                   >  4:299041/1‑139 (MQ=255)
                                                   aaaCACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGga                                                                                   <  3:299041/139‑1 (MQ=255)
                                                   aaaCACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTc                                                                          >  1:115877/1‑148 (MQ=255)
                                                     acacTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAt                                                                            >  1:792569/1‑144 (MQ=255)
                                                          acaaattatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTAATGGCACCGCTAACCCGCGATGCCAt                                                                                                           >  2:785351‑M1/78‑109 (MQ=255)
                                                            tCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAAtgtg                                                                                               <  1:703132/118‑1 (MQ=255)
                                                            tCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAAtgtg                                                                                               >  2:703132/1‑118 (MQ=255)
                                                              aGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAg                                                               <  2:395004/148‑1 (MQ=255)
                                                               gTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgt                                                              >  2:1065663/1‑148 (MQ=255)
                                                               gTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgt                                                              >  4:847740/1‑148 (MQ=255)
                                                                tatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACCATGGCACCGCTAACCCGCGATg                                                                                                               >  2:191821‑M1/72‑98 (MQ=255)
                                                                tatccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACCATGGCACCGCTAACCCGCGATg                                                                                                               <  1:191821‑M1/27‑1 (MQ=255)
                                                                tGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                                                          >  2:979456/1‑103 (MQ=255)
                                                                tGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                                                          <  1:979456/103‑1 (MQ=255)
                                                                  aGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgtg                                                             <  1:520510/146‑1 (MQ=255)
                                                                  aGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgtg                                                             >  2:520510/1‑146 (MQ=255)
                                                                  aGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGtgtg                                                           <  3:847740/148‑1 (MQ=255)
                                                                   ccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTATGGCACCGCTAACCCGCGAt                                                                                                                <  1:1031902‑M1/26‑1 (MQ=255)
                                                                   ccgttgcagttatgagtgacgtagatcacacttattcttcagaatcttcttccagctcgtcccacattGCACTATGGCACCGCTAACCCGCGAt                                                                                                                >  2:1031902‑M1/69‑94 (MQ=255)
                                                                     aTCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       <  1:1061176/133‑1 (MQ=255)
                                                                     aTCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       >  2:1061176/1‑133 (MQ=255)
                                                                     aTCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       <  3:884002/133‑1 (MQ=255)
                                                                     aTCTGTTACCCTGGTGTGGTGCGTTCCATGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTgg                                                                                    <  1:323318/120‑1 (MQ=255)
                                                                       cTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTa                                                      >  3:552320/1‑148 (MQ=255)
                                                                         gTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCcgcg                                                                                                                  <  2:188973/86‑1 (MQ=255)
                                                                         gTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCGATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTg                                                                        >  3:606763/1‑128 (MQ=255)
                                                                         gTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaagaa                                                                               <  4:453820/121‑1 (MQ=255)
                                                                          ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                                                          >  1:561126/1‑93 (MQ=255)
                                                                          ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGga                                                                                   >  2:315557/1‑116 (MQ=255)
                                                                          ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGga                                                                                   <  1:315557/116‑1 (MQ=255)
                                                                          ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       >  2:87729/1‑128 (MQ=255)
                                                                          ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       <  1:87729/128‑1 (MQ=255)
                                                                           tACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTa                                                                                                         <  3:1051564/93‑1 (MQ=255)
                                                                           tACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTa                                                      >  2:719965/1‑144 (MQ=255)
                                                                               cTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAAtg                                                                                                 <  4:233833/97‑1 (MQ=255)
                                                                                tggtgtggtgCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                                                          <  3:6622/87‑1 (MQ=255)
                                                                                tggtgtggtgCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTc                                                                          <  2:673163/119‑1 (MQ=255)
                                                                                tggtgtggtgCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       <  1:1010941/122‑1 (MQ=255)
                                                                                 ggtgtggtgCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGATGAtt                                                                           >  4:741284/1‑117 (MQ=255)
                                                                                 ggtgtggtgCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATa                                                                 <  4:1049619/127‑1 (MQ=255)
                                                                                 ggtgtggagCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATa                                                                 >  3:1049619/1‑127 (MQ=255)
                                                                                         gCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCaa                                    <  3:965223/148‑1 (MQ=255)
                                                                                            ttCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACgg                                 >  3:900899/1‑148 (MQ=255)
                                                                                                ttGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       <  2:118844/106‑1 (MQ=255)
                                                                                                   gCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAAtt                           >  3:291203/1‑147 (MQ=255)
                                                                                                     aaaGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGc                                                                                       <  2:953388/85‑1 (MQ=255)
                                                                                                     aaaGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGc                                                                                       >  1:953388/1‑85 (MQ=255)
                                                                                                     aaaGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgt                        <  4:552320/148‑1 (MQ=255)
                                                                                                      aaGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGa                                <  2:33999/139‑1 (MQ=255)
                                                                                                      aaGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgtt                       <  2:850421/148‑1 (MQ=255)
                                                                                                       aGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACTGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTg                                                                        <  1:727717/98‑1 (MQ=255)
                                                                                                               cccATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgt                        >  4:126799/1‑138 (MQ=255)
                                                                                                                 cATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGc                                                                                       >  1:55677/1‑73 (MQ=255)
                                                                                                                 cATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGc                                                                                       <  2:55677/73‑1 (MQ=255)
                                                                                                                 cATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttg                      <  2:261260/138‑1 (MQ=255)
                                                                                                                    gCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAAtgt                                                                                                <  1:249542/61‑1 (MQ=255)
                                                                                                                    gCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgtg                                                             <  4:336475/96‑1 (MQ=255)
                                                                                                                    gCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTc                                                  >  1:479630/1‑107 (MQ=255)
                                                                                                                    gCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTc                                                  <  2:479630/107‑1 (MQ=255)
                                                                                                                        ccACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGa                                                          >  3:858687/1‑95 (MQ=255)
                                                                                                                        ccACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGa                                                          <  4:858687/95‑1 (MQ=255)
                                                                                                                           cGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTg                                                                        >  4:616595/1‑78 (MQ=255)
                                                                                                                           cGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCa                                     >  1:995506/1‑113 (MQ=255)
                                                                                                                           cGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCa                                     <  2:995506/113‑1 (MQ=255)
                                                                                                                           cGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttgt                     <  4:296119/129‑1 (MQ=255)
                                                                                                                           cGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCg  <  2:232968/148‑1 (MQ=255)
                                                                                                                             ccTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATa                              >  2:883667/1‑118 (MQ=255)
                                                                                                                             ccTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttgttg                   >  1:359927/1‑129 (MQ=255)
                                                                                                                              cTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTga                                                                       >  1:877432/1‑76 (MQ=255)
                                                                                                                               tttCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCa                 >  3:174873/1‑129 (MQ=255)
                                                                                                                                ttCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATaaa             >  4:677509/1‑132 (MQ=255)
                                                                                                                                ttCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATaaa             <  3:677509/132‑1 (MQ=255)
                                                                                                                                        CACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgt                        <  4:274918‑M1/113‑1 (MQ=255)
                                                                                                                                        CACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgt                        >  3:274918‑M1/1‑113 (MQ=255)
                                                                                                                                         ACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttg                      >  4:900892‑M1/1‑114 (MQ=255)
                                                                                                                                          CCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttgt                     >  2:986275‑M1/1‑114 (MQ=255)
                                                                                                                                          CCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttgt                     <  1:986275‑M1/114‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                  
CAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCG  >  NC_000913/1099986‑1100256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: