Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,295:1 +C 7.3% coding (228/1773 nt) aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,2951.C 7.3% 239.0 / 13.8 55coding (228/1773 nt)aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (26/25);  new base C (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

TTCAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGAAGATCAGGCTACCAAGAT  >  NC_000913/1950155‑1950437
                                                                                                                                             |                                                                                                                                              
ttCAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACgg                                                                                                                                         >  3:17124/1‑148 (MQ=255)
  cAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTg                                                                                                                                       <  3:890017/148‑1 (MQ=255)
      cgcgCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                                                                   <  4:17124/148‑1 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 <  1:450256/148‑1 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 >  2:908515/1‑148 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 >  2:450256/1‑148 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 <  1:908515/148‑1 (MQ=255)
            tcttcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACg                                                                                                                                          <  1:362107/135‑1 (MQ=255)
            tcttcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACg                                                                                                                                          >  2:362107/1‑135 (MQ=255)
               tcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 <  1:227920/141‑1 (MQ=255)
               tcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 >  2:227920/1‑141 (MQ=255)
                             gTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             <  2:958277/147‑1 (MQ=255)
                             gTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             >  1:958277/1‑147 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTa                                                                                                                                            <  1:118705/114‑1 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTa                                                                                                                                            >  2:118705/1‑114 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGc                                                                                                                       >  2:650133/1‑135 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGc                                                                                                                       <  1:650133/135‑1 (MQ=255)
                                                    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGaa                                                                                                                          <  1:480160/111‑1 (MQ=255)
                                                    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGaa                                                                                                                          >  2:480160/1‑111 (MQ=255)
                                                               atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             <  1:35180/113‑1 (MQ=255)
                                                               atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             >  2:35180/1‑113 (MQ=255)
                                                                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                                                                   <  4:569453/83‑1 (MQ=255)
                                                                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                                                                   >  3:569453/1‑83 (MQ=255)
                                                                        aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                                                                 >  3:1092208/1‑148 (MQ=255)
                                                                        aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                                                                 >  3:769980/1‑148 (MQ=255)
                                                                                 gCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                                                        <  4:1092208/148‑1 (MQ=255)
                                                                                    aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 <  2:452456/72‑1 (MQ=255)
                                                                                    aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                                                 >  1:452456/1‑72 (MQ=255)
                                                                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTa                                                                                                                                            <  2:636766/56‑1 (MQ=255)
                                                                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTa                                                                                                                                            >  1:636766/1‑56 (MQ=255)
                                                                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCag                                                                                                  >  3:185188/1‑97 (MQ=255)
                                                                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCag                                                                                                  <  4:185188/97‑1 (MQ=255)
                                                                                           cGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGAt                                              >  1:224685/1‑148 (MQ=255)
                                                                                               ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAcc                                                                                                                              >  3:357628/1‑64 (MQ=255)
                                                                                               ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAcc                                                                                                                              <  4:357628/64‑1 (MQ=255)
                                                                                                  accGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTcg                                       >  4:870165/2‑148 (MQ=255)
                                                                                                      gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                                                                 >  1:1017066/1‑102 (MQ=255)
                                                                                                      gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                                                                 <  2:1017066/102‑1 (MQ=255)
                                                                                                             tatCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCat                            <  2:224685/148‑1 (MQ=255)
                                                                                                                cgcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                                                                     >  2:676551/1‑88 (MQ=255)
                                                                                                                cgcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                                                                     <  1:676551/88‑1 (MQ=255)
                                                                                                                      tAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGaa                   <  4:769980/148‑1 (MQ=255)
                                                                                                                            ttttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                                                        <  2:29244/105‑1 (MQ=255)
                                                                                                                            ttttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                                                        >  1:29244/1‑105 (MQ=255)
                                                                                                                                    tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATc                         <  4:889016/128‑1 (MQ=255)
                                                                                                                                    tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATc                         >  3:889016/1‑128 (MQ=255)
                                                                                                                                     cACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATc                                                                      >  3:717135/1‑82 (MQ=37)
                                                                                                                                     cACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATc                                                                      <  4:717135/82‑1 (MQ=37)
                                                                                                                                     cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCATAGGCCAGCTTTa                                                                                      <  4:736468/66‑1 (MQ=255)
                                                                                                                                     cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                                                                      >  3:736468/1‑66 (MQ=255)
                                                                                                                                     cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAATTACCTGCACg                                                <  3:668339/104‑1 (MQ=255)
                                                                                                                                     cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                                                      <  4:400306/98‑1 (MQ=255)
                                                                                                                                     cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                                                      >  3:400306/1‑98 (MQ=255)
                                                                                                                                     cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACg                                                >  4:668339/1‑104 (MQ=255)
                                                                                                                                       cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGAAGATCAGGCTACCAAGAt  >  2:377113/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TTCAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGAAGATCAGGCTACCAAGAT  >  NC_000913/1950155‑1950437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑