Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,092,774:1 +A 5.5% coding (897/951 nt) gshB → glutathione synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,092,7741.A 5.5% 658.7 / 31.1 146coding (897/951 nt)gshBglutathione synthetase
Reads supporting (aligned to +/- strand):  ref base . (69/69);  new base A (4/4);  total (73/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

AAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGT  >  NC_000913/3092634‑3092902
                                                                                                                                             |                                                                                                                                
  aaTCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCAcc                                                                                                                         <  1:605710/148‑1 (MQ=255)
                tCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGa                                                                                                           <  4:992178/148‑1 (MQ=255)
                           cTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCa                                                                                                                           >  1:76715/1‑121 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGCTATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAAt                                                                                                              <  3:693635/128‑1 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATc                                                                                                                            <  2:661334/114‑1 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATc                                                                                                                            >  1:661334/1‑114 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTa                                                                                                                <  2:590338/126‑1 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTa                                                                                                                >  1:590338/1‑126 (MQ=255)
                                 gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAAt                                                                                                              >  4:693635/1‑128 (MQ=255)
                                    aaaGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAc                                                                                       <  3:1085470/148‑1 (MQ=255)
                                         gCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGCGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGaa                                                                                                                     <  1:230481/113‑1 (MQ=255)
                                         gCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGaa                                                                                                                     >  2:230481/1‑113 (MQ=255)
                                          cTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTa                                                                                        >  1:835871/1‑141 (MQ=255)
                                           tGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                >  1:1043443/1‑148 (MQ=255)
                                            gATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGa                                                                                                           <  1:675509/120‑1 (MQ=255)
                                             aTTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                >  4:200047/1‑146 (MQ=255)
                                             aTTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                <  3:200047/146‑1 (MQ=255)
                                               ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGt                                                                                           <  3:99535/133‑1 (MQ=255)
                                                     tgGTCTGGATATCATCAGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGa                                                                                                                      >  4:1074456/1‑100 (MQ=255)
                                                        tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCAc                                                                                                                          <  4:603779/93‑1 (MQ=255)
                                                        tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCAc                                                                                                                          >  3:603779/1‑93 (MQ=255)
                                                        tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAAcc                                                                          <  1:759334/141‑1 (MQ=255)
                                                        tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAAcc                                                                          >  2:759334/1‑141 (MQ=255)
                                                         cTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGa                                                                                                  <  2:785273/116‑1 (MQ=255)
                                                         cTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                              >  2:744801/1‑120 (MQ=255)
                                                           ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                               >  3:150651/1‑101 (MQ=255)
                                                           ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                               <  4:150651/101‑1 (MQ=255)
                                                           ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCg                                                                <  2:1043443/148‑1 (MQ=255)
                                                            gATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga                                                               >  2:1062012/1‑148 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                              <  3:323362/110‑1 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                              >  2:811605/1‑110 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                              >  4:323362/1‑110 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCa                                                                                              <  1:811605/110‑1 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                 <  3:890185/123‑1 (MQ=255)
                                                                   tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAt                                                        >  1:902654/1‑148 (MQ=255)
                                                                    cGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATTGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtc                                                       >  1:194078/1‑148 (MQ=255)
                                                                       cGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagc                                                                                    <  4:780116/116‑1 (MQ=255)
                                                                        gACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGTAGGATCTCGt                                                   <  3:652422/148‑1 (MQ=255)
                                                                        gACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                   <  2:394304/148‑1 (MQ=255)
                                                                         aCCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                               >  3:739417/1‑87 (MQ=255)
                                                                         aCCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTa                                                                                        <  4:534772/110‑1 (MQ=255)
                                                                         aCCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTa                                                                                        >  3:534772/1‑110 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGt                                                                                           >  1:1045062/1‑102 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                 >  4:1088729/1‑112 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                 <  3:1088729/112‑1 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCtt                                                                    <  4:1063224/125‑1 (MQ=255)
                                                                              ctgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCtt                                                                    >  3:1063224/1‑125 (MQ=255)
                                                                                gactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGActc                                           <  1:1094218/148‑1 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagca                                                                                   >  4:336553/1‑104 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                            <  4:10054/127‑1 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                   >  2:217267/1‑136 (MQ=255)
                                                                                    gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTg                                                 <  3:21283/138‑1 (MQ=255)
                                                                                      aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                  >  1:384644/1‑119 (MQ=255)
                                                                                        ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                   <  1:495791/148‑1 (MQ=255)
                                                                                         tAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAc                                                                                       >  1:233326/1‑95 (MQ=255)
                                                                                         tAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCAACGAAGCACGTTTAc                                                                                       <  4:843717/95‑1 (MQ=255)
                                                                                            cGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                         <  3:1055971/122‑1 (MQ=255)
                                                                                            cGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTg                                         <  3:293520/138‑1 (MQ=255)
                                                                                               cACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGa                                                                                                                      >  4:740168/1‑58 (MQ=255)
                                                                                               cACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGa                                                                                                                      <  3:740168/58‑1 (MQ=255)
                                                                                                aCCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                        <  2:875847/135‑1 (MQ=255)
                                                                                                aCCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                        >  1:875847/1‑135 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGc                                                                                                        >  3:548891/1‑70 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                         <  2:244535/117‑1 (MQ=255)
                                                                                                 ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                        <  4:703150/134‑1 (MQ=255)
                                                                                                  cAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                               >  4:82063/1‑62 (MQ=255)
                                                                                                  cAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTaa                                                                                                               <  3:82063/62‑1 (MQ=255)
                                                                                                  cAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAt                                                        >  3:1120263/1‑117 (MQ=255)
                                                                                                  cAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAg                                       >  1:315541/1‑134 (MQ=255)
                                                                                                   aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCtt                                                                    <  4:417926/104‑1 (MQ=255)
                                                                                                      ccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                            >  1:1083056/1‑109 (MQ=255)
                                                                                                      ccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                            <  2:1083056/109‑1 (MQ=255)
                                                                                                        aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                   >  4:1095136/1‑116 (MQ=255)
                                                                                                            tGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTg                                                 >  2:559421/1‑114 (MQ=255)
                                                                                                               aTTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                   >  1:285339/1‑141 (MQ=255)
                                                                                                               aTTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                   <  2:285339/141‑1 (MQ=255)
                                                                                                                 tCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTa                                                                             >  2:325952/1‑81 (MQ=255)
                                                                                                                         ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAAccc                                                                         <  1:989162/77‑1 (MQ=255)
                                                                                                                         ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAAccc                                                                         >  2:989162/1‑77 (MQ=255)
                                                                                                                         ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt  <  2:194078/148‑1 (MQ=255)
                                                                                                                          tGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGGGTGACAGCGCCCTTCTTTCCACGCATACTg          >  2:970984/1‑139 (MQ=255)
                                                                                                                               cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGaa                                                            >  4:63996/1‑84 (MQ=255)
                                                                                                                               cAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                        >  2:191159/1‑104 (MQ=255)
                                                                                                                                taacattCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt  >  4:19341/6‑141 (MQ=255)
                                                                                                                                agagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                  <  3:791386/77‑1 (MQ=255)
                                                                                                                                agagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTg                                     >  1:350809/1‑106 (MQ=255)
                                                                                                                                agagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTg                                     <  2:350809/106‑1 (MQ=255)
                                                                                                                                   gTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATAc            >  4:280412/1‑128 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                   <  2:632680/87‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg          >  4:975966/1‑128 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg          >  1:395377/1‑128 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg          <  3:975966/128‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg          <  2:395377/128‑1 (MQ=255)
                                                                                                                                     ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgcgc     <  3:36070/133‑1 (MQ=255)
                                                                                                                                      tCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGt                                                   <  2:859119/86‑1 (MQ=255)
                                                                                                                                      tCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTg                                         >  1:204039/1‑96 (MQ=255)
                                                                                                                                      tCCGGTGATCGACACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt  >  4:537708/1‑135 (MQ=255)
                                                                                                                                           tgtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                   <  4:411801/97‑1 (MQ=255)
                                                                                                                                           tgtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                   >  3:411801/1‑97 (MQ=255)
                                                                                                                                            gtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg          <  3:468203/121‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                
AAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGT  >  NC_000913/3092634‑3092902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: