Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 328,379 G→T 12.0% L119M (CTG→ATG) ‡ betB ← betaine aldehyde dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913328,3790GT12.0% 271.0 / 21.1 83L119M (CTG→ATG) ‡betBbetaine aldehyde dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base T (6/4);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGC  >  NC_000913/328240‑328511
                                                                                                                                           |                                                                                                                                    
ggggCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCgt                                                                                                                              <  3:717104/148‑1 (MQ=255)
         ttCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCgg                                                                                                                                 >  1:1016240/1‑136 (MQ=255)
         ttCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCgg                                                                                                                                 <  2:1016240/136‑1 (MQ=255)
             acaGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGcc                                                                                                                                    <  3:14161/129‑1 (MQ=255)
             acaGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGcc                                                                                                                                    >  4:14161/1‑129 (MQ=255)
                     aTCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGGGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCCCGTCCGCAc                                                                                                         >  4:826913/1‑148 (MQ=255)
                           aTCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCgtagta                                                                                                                          >  4:769571/1‑125 (MQ=255)
                           aTCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCgtagta                                                                                                                          <  3:769571/125‑1 (MQ=255)
                                 tAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCGGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGatat                                                                                             >  4:731707/1‑148 (MQ=255)
                                       cATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGCTGATCCCgg                                                                                                                                 >  2:393730/1‑106 (MQ=255)
                                       cATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGCTGATCCCgg                                                                                                                                 <  3:695611/106‑1 (MQ=255)
                                       cATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGCTGATCCCgg                                                                                                                                 >  4:695611/1‑106 (MQ=255)
                                        aTGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGt                                                                                      >  4:1004309/1‑148 (MQ=255)
                                           cgcCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGa                                                                                   <  3:826913/148‑1 (MQ=255)
                                             ccAATCCCTGCCCCTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACg                                                                                                                <  3:96922/117‑1 (MQ=255)
                                             ccAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACg                                                                                                                >  4:96922/1‑117 (MQ=255)
                                                aTCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGttt                                                                              <  3:1004309/148‑1 (MQ=255)
                                                          cTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGc                                                                    <  3:731707/148‑1 (MQ=255)
                                                             cGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTc                                                                             <  1:105023/136‑1 (MQ=255)
                                                             cGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTc                                                                             >  2:105023/1‑136 (MQ=255)
                                                                  aGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTaa                                                                                                   >  1:762797/1‑109 (MQ=255)
                                                                  aGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTaa                                                                                                   <  2:762797/109‑1 (MQ=255)
                                                                   gCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTGGTACTCCAGCACGTCCGCACCGGTAACGata                                                                                              <  4:188907/113‑1 (MQ=255)
                                                                   gCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTGGTACTCCAGCACGTCCGCACCGGTAACGata                                                                                              >  3:188907/1‑113 (MQ=255)
                                                                      gTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACgg                                                                                       <  3:20657/117‑1 (MQ=255)
                                                                      gTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACgg                                                                                       >  4:20657/1‑117 (MQ=255)
                                                                        tCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGatat                                                                                             >  3:151739/1‑109 (MQ=255)
                                                                        tCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGatat                                                                                             <  4:151739/109‑1 (MQ=255)
                                                                           cggcggGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat                                                                                               <  3:321041/104‑1 (MQ=255)
                                                                           cggcggGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat                                                                                               >  4:321041/1‑104 (MQ=255)
                                                                                   ataCACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat                                                                                               <  4:1075338/96‑1 (MQ=255)
                                                                                   ataCACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGat                                                                                               >  3:1075338/1‑96 (MQ=255)
                                                                                   ataCACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGt                                           >  3:901711/1‑148 (MQ=255)
                                                                                     acacAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTAGTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAg                                                     >  3:229712/1‑136 (MQ=255)
                                                                                     acacAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTAGTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAg                                                     <  4:229712/136‑1 (MQ=255)
                                                                                      cacaAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGa                                                                                          >  4:166144/1‑98 (MQ=255)
                                                                                      cacaAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGa                                                                                          <  3:166144/98‑1 (MQ=255)
                                                                                       acaAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTg                                       <  4:901711/148‑1 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACt                                                                                                                        <  1:576271/66‑1 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACt                                                                                                                        >  2:576271/1‑66 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAg                                                                                                                    >  1:545243/1‑70 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAg                                                                                                                    <  2:545243/70‑1 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACg                                                                                        >  4:174319/1‑98 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACg                                                                                        <  3:174319/98‑1 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg                                                    >  2:554398/1‑134 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg                                                    <  1:554398/134‑1 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTc                                               >  1:1095342/1‑139 (MQ=255)
                                                                                        caAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTc                                               <  2:1095342/139‑1 (MQ=255)
                                                                                               cGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAatat                                                                      >  2:704659/1‑109 (MQ=255)
                                                                                               cGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAatat                                                                      <  1:704659/109‑1 (MQ=255)
                                                                                                                 cTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTg                                                                                    <  1:91387/77‑1 (MQ=255)
                                                                                                                 cTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTg                                                                                    >  2:91387/1‑77 (MQ=255)
                                                                                                                     cTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt                                                                  <  3:618219/91‑1 (MQ=255)
                                                                                                                     cTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt                                                                  >  2:604845/1‑91 (MQ=255)
                                                                                                                     cTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt                                                                  <  1:604845/91‑1 (MQ=255)
                                                                                                                     cTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCtt                                                                  >  4:618219/1‑91 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGt                                                                                <  3:281882/71‑1 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGt                                                                                >  4:281882/1‑71 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTcc                                                                            >  3:671129/1‑75 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTcc                                                                            <  4:671129/75‑1 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGt                                                  >  3:124038/1‑101 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGt                                                  <  4:124038/101‑1 (MQ=255)
                                                                                                                           tCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACgg   >  4:830062/1‑148 (MQ=255)
                                                                                                                            ccAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACg    <  4:616173/146‑1 (MQ=255)
                                                                                                                            ccAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACg    >  3:616173/1‑146 (MQ=255)
                                                                                                                            ccAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGc  <  3:830062/148‑1 (MQ=255)
                                                                                                                               acgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg                                                    >  4:399940/2‑95 (MQ=255)
                                                                                                                               acgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAgg                                                    <  3:399940/94‑1 (MQ=255)
                                                                                                                               acgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTg                                       >  3:876817/2‑108 (MQ=255)
                                                                                                                               acgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTg                                       <  4:876817/107‑1 (MQ=255)
                                                                                                                               acgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt                           >  1:162621/2‑120 (MQ=255)
                                                                                                                               acgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt                           <  2:162621/119‑1 (MQ=255)
                                                                                                                                cgcCGGGCTGATCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGttt                                                >  3:116179/1‑98 (MQ=255)
                                                                                                                                  ccGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAat                                                                        >  2:79495/1‑72 (MQ=255)
                                                                                                                                  ccGGCATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAat                                                                        <  1:79495/72‑1 (MQ=255)
                                                                                                                                    gggATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGa                                                      >  1:794005/1‑88 (MQ=255)
                                                                                                                                    gggATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGa                                                      <  2:794005/88‑1 (MQ=255)
                                                                                                                                      gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCgg                                                            >  1:293060/1‑80 (MQ=255)
                                                                                                                                      gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCgg                                                            <  2:293060/80‑1 (MQ=255)
                                                                                                                                      gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt                           <  1:602856/113‑1 (MQ=255)
                                                                                                                                      gATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCAt                           >  2:602856/1‑113 (MQ=255)
                                                                                                                                         cAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTg                         <  4:1139329/112‑1 (MQ=255)
                                                                                                                                         cAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTg                         >  3:1139329/1‑112 (MQ=255)
                                                                                                                                           |                                                                                                                                    
GGGGCGGATTTCCACAGGGCAATCTGGATCGGGTAGTTCCATGCGCCAATCCCTGCCACTACGCCCAGCGGTTCGCGGCGGGTATACACAAAGGACGTTTCACGCAACGGGATCTGGCTGCCTTCCAGCGCCGGGATCAGCCCGGCGTAGTACTCCAGCACGTCCGCACCGGTAACGATATCGACGGTTGAGGTTTCCGAATATGCTTTTCCGGTGTCGAGGGTTTCCAGTTTTGCGAGTTCGTCATTGCGTTCACGCAGAATATCAACGGC  >  NC_000913/328240‑328511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑